New low-viscosity overlay medium for viral plaque assays View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-12

AUTHORS

Mikhail Matrosovich, Tatyana Matrosovich, Wolfgang Garten, Hans-Dieter Klenk

ABSTRACT

BACKGROUND: Plaque assays in cell culture monolayers under solid or semisolid overlay media are commonly used for quantification of viruses and antiviral substances. To overcome the pitfalls of known overlays, we tested suspensions of microcrystalline cellulose Avicel RC/CLtrade mark as overlay media in the plaque and plaque-inhibition assay of influenza viruses. RESULTS: Significantly larger plaques were formed under Avicel-containing media, as compared to agar and methylcellulose (MC) overlay media. The plaque size increased with decreasing Avicel concentration, but even very diluted Avicel overlays (0.3%) ensured formation of localized plaques. Due to their low viscosity, Avicel overlays were easier to use than methylcellulose overlays, especially in the 96-well culture plates. Furthermore, Avicel overlay could be applied without prior removal of the virus inoculum thus facilitating the assay and reducing chances of cross-contamination. Using neuraminidase inhibitor oseltamivir carboxylate, we demonstrated applicability of the Avicel-based plaque reduction assay for testing of antiviral substances. CONCLUSION: Plaque assay under Avicel-containing overlay media is easier, faster and more sensitive than assays under agar- and methylcellulose overlays. The assay can be readily performed in a 96-well plate format and seems particularly suitable for high-throughput virus titrations, serological studies and experiments on viral drug sensitivity. It may also facilitate work with highly pathogenic agents performed under hampered conditions of bio-safety labs. More... »

PAGES

63

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1743-422x-3-63

DOI

http://dx.doi.org/10.1186/1743-422x-3-63

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000911842

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16945126


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Agar", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cellulose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dogs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Influenza A virus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Methylcellulose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viral Plaque Assay", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viscosity", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Philipp University of Marburg", 
          "id": "https://www.grid.ac/institutes/grid.10253.35", 
          "name": [
            "Institute of Virology, Philipps University, Hans-Meerwein-Str. 3, 35043, Marburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matrosovich", 
        "givenName": "Mikhail", 
        "id": "sg:person.01343477751.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343477751.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Philipp University of Marburg", 
          "id": "https://www.grid.ac/institutes/grid.10253.35", 
          "name": [
            "Institute of Virology, Philipps University, Hans-Meerwein-Str. 3, 35043, Marburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matrosovich", 
        "givenName": "Tatyana", 
        "id": "sg:person.01123740174.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123740174.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Philipp University of Marburg", 
          "id": "https://www.grid.ac/institutes/grid.10253.35", 
          "name": [
            "Institute of Virology, Philipps University, Hans-Meerwein-Str. 3, 35043, Marburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Garten", 
        "givenName": "Wolfgang", 
        "id": "sg:person.01153160064.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153160064.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Philipp University of Marburg", 
          "id": "https://www.grid.ac/institutes/grid.10253.35", 
          "name": [
            "Institute of Virology, Philipps University, Hans-Meerwein-Str. 3, 35043, Marburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Klenk", 
        "givenName": "Hans-Dieter", 
        "id": "sg:person.0610701656.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610701656.34"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1128/jvi.77.15.8418-8425.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009892760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-1-4832-3219-5.50012-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024080848"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/viro.1998.9274", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041327681"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-12", 
    "datePublishedReg": "2006-12-01", 
    "description": "BACKGROUND: Plaque assays in cell culture monolayers under solid or semisolid overlay media are commonly used for quantification of viruses and antiviral substances. To overcome the pitfalls of known overlays, we tested suspensions of microcrystalline cellulose Avicel RC/CLtrade mark as overlay media in the plaque and plaque-inhibition assay of influenza viruses.\nRESULTS: Significantly larger plaques were formed under Avicel-containing media, as compared to agar and methylcellulose (MC) overlay media. The plaque size increased with decreasing Avicel concentration, but even very diluted Avicel overlays (0.3%) ensured formation of localized plaques. Due to their low viscosity, Avicel overlays were easier to use than methylcellulose overlays, especially in the 96-well culture plates. Furthermore, Avicel overlay could be applied without prior removal of the virus inoculum thus facilitating the assay and reducing chances of cross-contamination. Using neuraminidase inhibitor oseltamivir carboxylate, we demonstrated applicability of the Avicel-based plaque reduction assay for testing of antiviral substances.\nCONCLUSION: Plaque assay under Avicel-containing overlay media is easier, faster and more sensitive than assays under agar- and methylcellulose overlays. The assay can be readily performed in a 96-well plate format and seems particularly suitable for high-throughput virus titrations, serological studies and experiments on viral drug sensitivity. It may also facilitate work with highly pathogenic agents performed under hampered conditions of bio-safety labs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1743-422x-3-63", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1034449", 
        "issn": [
          "1743-422X"
        ], 
        "name": "Virology Journal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "3"
      }
    ], 
    "name": "New low-viscosity overlay medium for viral plaque assays", 
    "pagination": "63", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "0457edf7b503906d5b00bd507d77047a877e636f917ee63558b4d15681c95336"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16945126"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101231645"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1743-422x-3-63"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1000911842"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1743-422x-3-63", 
      "https://app.dimensions.ai/details/publication/pub.1000911842"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T01:06", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000509.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1743-422X-3-63"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-63'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-63'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-63'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-63'


 

This table displays all metadata directly associated to this object as RDF triples.

142 TRIPLES      21 PREDICATES      43 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1743-422x-3-63 schema:about N12f43e02c82d42918a187ff070e3a7a9
2 N4ced9613e866456ca139d388f70c09a5
3 N53f0a1da333743e7bfcb101f96e2d2d1
4 N5d744d9d77424c0fafee41aeb34c7ecc
5 N62517e56aaac46429588f3ecc0ae9eb4
6 N784eff4b46164bcc8ff31edd06c12054
7 N97886fdc578541ada1c00131e839d34d
8 Nd16df151143c4baf8472258074360363
9 Neb635927e203403e8f46889174d64a04
10 Nf6d5af8dc1224dbd800a9b2d740c4911
11 Nffe99e1ee5e0455aab875474eaad52ac
12 anzsrc-for:11
13 anzsrc-for:1108
14 schema:author Nb40a972f6483401392fa528c252f5b75
15 schema:citation https://doi.org/10.1006/viro.1998.9274
16 https://doi.org/10.1016/b978-1-4832-3219-5.50012-3
17 https://doi.org/10.1128/jvi.77.15.8418-8425.2003
18 schema:datePublished 2006-12
19 schema:datePublishedReg 2006-12-01
20 schema:description BACKGROUND: Plaque assays in cell culture monolayers under solid or semisolid overlay media are commonly used for quantification of viruses and antiviral substances. To overcome the pitfalls of known overlays, we tested suspensions of microcrystalline cellulose Avicel RC/CLtrade mark as overlay media in the plaque and plaque-inhibition assay of influenza viruses. RESULTS: Significantly larger plaques were formed under Avicel-containing media, as compared to agar and methylcellulose (MC) overlay media. The plaque size increased with decreasing Avicel concentration, but even very diluted Avicel overlays (0.3%) ensured formation of localized plaques. Due to their low viscosity, Avicel overlays were easier to use than methylcellulose overlays, especially in the 96-well culture plates. Furthermore, Avicel overlay could be applied without prior removal of the virus inoculum thus facilitating the assay and reducing chances of cross-contamination. Using neuraminidase inhibitor oseltamivir carboxylate, we demonstrated applicability of the Avicel-based plaque reduction assay for testing of antiviral substances. CONCLUSION: Plaque assay under Avicel-containing overlay media is easier, faster and more sensitive than assays under agar- and methylcellulose overlays. The assay can be readily performed in a 96-well plate format and seems particularly suitable for high-throughput virus titrations, serological studies and experiments on viral drug sensitivity. It may also facilitate work with highly pathogenic agents performed under hampered conditions of bio-safety labs.
21 schema:genre research_article
22 schema:inLanguage en
23 schema:isAccessibleForFree true
24 schema:isPartOf N0f6e549506bc4779a4edf1c671b61512
25 N5cf8f2f0b745430593187d7e32d2e8d5
26 sg:journal.1034449
27 schema:name New low-viscosity overlay medium for viral plaque assays
28 schema:pagination 63
29 schema:productId N4b63862b1583470583c873bfe2b72093
30 N6780d59ba442495c8a6bf32c1a2ce556
31 N6800b4a2143c40c29ba1ba9c3ea01aad
32 Nc05e4e40811043beb02f1a627a658c5d
33 Nf9410b54c5ed4f5ca4f3f3dce5c827dc
34 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000911842
35 https://doi.org/10.1186/1743-422x-3-63
36 schema:sdDatePublished 2019-04-11T01:06
37 schema:sdLicense https://scigraph.springernature.com/explorer/license/
38 schema:sdPublisher N21743dddc47e407e89e02caddf08f32f
39 schema:url http://link.springer.com/10.1186%2F1743-422X-3-63
40 sgo:license sg:explorer/license/
41 sgo:sdDataset articles
42 rdf:type schema:ScholarlyArticle
43 N0a2e5149445241d88d559fd86e7e3436 rdf:first sg:person.01153160064.05
44 rdf:rest N23ff4899007c4cef9f5edb0e64627033
45 N0f6e549506bc4779a4edf1c671b61512 schema:volumeNumber 3
46 rdf:type schema:PublicationVolume
47 N12f43e02c82d42918a187ff070e3a7a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Cellulose
49 rdf:type schema:DefinedTerm
50 N21743dddc47e407e89e02caddf08f32f schema:name Springer Nature - SN SciGraph project
51 rdf:type schema:Organization
52 N23ff4899007c4cef9f5edb0e64627033 rdf:first sg:person.0610701656.34
53 rdf:rest rdf:nil
54 N4b63862b1583470583c873bfe2b72093 schema:name readcube_id
55 schema:value 0457edf7b503906d5b00bd507d77047a877e636f917ee63558b4d15681c95336
56 rdf:type schema:PropertyValue
57 N4ced9613e866456ca139d388f70c09a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
58 schema:name Cell Line
59 rdf:type schema:DefinedTerm
60 N53f0a1da333743e7bfcb101f96e2d2d1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
61 schema:name Influenza A virus
62 rdf:type schema:DefinedTerm
63 N5cf8f2f0b745430593187d7e32d2e8d5 schema:issueNumber 1
64 rdf:type schema:PublicationIssue
65 N5d744d9d77424c0fafee41aeb34c7ecc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Culture Media
67 rdf:type schema:DefinedTerm
68 N62517e56aaac46429588f3ecc0ae9eb4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Viscosity
70 rdf:type schema:DefinedTerm
71 N666cc5952aea487798a06d1c457af129 rdf:first sg:person.01123740174.26
72 rdf:rest N0a2e5149445241d88d559fd86e7e3436
73 N6780d59ba442495c8a6bf32c1a2ce556 schema:name nlm_unique_id
74 schema:value 101231645
75 rdf:type schema:PropertyValue
76 N6800b4a2143c40c29ba1ba9c3ea01aad schema:name dimensions_id
77 schema:value pub.1000911842
78 rdf:type schema:PropertyValue
79 N784eff4b46164bcc8ff31edd06c12054 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Dogs
81 rdf:type schema:DefinedTerm
82 N97886fdc578541ada1c00131e839d34d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Methylcellulose
84 rdf:type schema:DefinedTerm
85 Nb40a972f6483401392fa528c252f5b75 rdf:first sg:person.01343477751.83
86 rdf:rest N666cc5952aea487798a06d1c457af129
87 Nc05e4e40811043beb02f1a627a658c5d schema:name pubmed_id
88 schema:value 16945126
89 rdf:type schema:PropertyValue
90 Nd16df151143c4baf8472258074360363 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Agar
92 rdf:type schema:DefinedTerm
93 Neb635927e203403e8f46889174d64a04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Humans
95 rdf:type schema:DefinedTerm
96 Nf6d5af8dc1224dbd800a9b2d740c4911 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Viral Plaque Assay
98 rdf:type schema:DefinedTerm
99 Nf9410b54c5ed4f5ca4f3f3dce5c827dc schema:name doi
100 schema:value 10.1186/1743-422x-3-63
101 rdf:type schema:PropertyValue
102 Nffe99e1ee5e0455aab875474eaad52ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Animals
104 rdf:type schema:DefinedTerm
105 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
106 schema:name Medical and Health Sciences
107 rdf:type schema:DefinedTerm
108 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
109 schema:name Medical Microbiology
110 rdf:type schema:DefinedTerm
111 sg:journal.1034449 schema:issn 1743-422X
112 schema:name Virology Journal
113 rdf:type schema:Periodical
114 sg:person.01123740174.26 schema:affiliation https://www.grid.ac/institutes/grid.10253.35
115 schema:familyName Matrosovich
116 schema:givenName Tatyana
117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123740174.26
118 rdf:type schema:Person
119 sg:person.01153160064.05 schema:affiliation https://www.grid.ac/institutes/grid.10253.35
120 schema:familyName Garten
121 schema:givenName Wolfgang
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153160064.05
123 rdf:type schema:Person
124 sg:person.01343477751.83 schema:affiliation https://www.grid.ac/institutes/grid.10253.35
125 schema:familyName Matrosovich
126 schema:givenName Mikhail
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343477751.83
128 rdf:type schema:Person
129 sg:person.0610701656.34 schema:affiliation https://www.grid.ac/institutes/grid.10253.35
130 schema:familyName Klenk
131 schema:givenName Hans-Dieter
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610701656.34
133 rdf:type schema:Person
134 https://doi.org/10.1006/viro.1998.9274 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041327681
135 rdf:type schema:CreativeWork
136 https://doi.org/10.1016/b978-1-4832-3219-5.50012-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024080848
137 rdf:type schema:CreativeWork
138 https://doi.org/10.1128/jvi.77.15.8418-8425.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009892760
139 rdf:type schema:CreativeWork
140 https://www.grid.ac/institutes/grid.10253.35 schema:alternateName Philipp University of Marburg
141 schema:name Institute of Virology, Philipps University, Hans-Meerwein-Str. 3, 35043, Marburg, Germany
142 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...