Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-04-22

AUTHORS

Michael P Boyle, Raymond A Enke, Jeffrey B Reynolds, Peter J Mogayzel, William B Guggino, Pamela L Zeitlin

ABSTRACT

BACKGROUND: Adeno-associated virus type 2 (AAV-2) attachment and internalization is thought to be mediated by host cell membrane-associated heparan sulfate proteoglycans (HSPG). Lack of HSPG on the apical membrane of respiratory epithelial cells has been identified as a reason for inefficient rAAV-2 infection in pulmonary applications in-vivo. The aim of this investigation was to determine the necessity of cell membrane HSPG for efficient infection by rAAV-2. RESULTS: Rates of transduction with rAAV2-CMV-EGFP3 in several different immortalized airway epithelial cell lines were determined at different multiplicities of infection (MOI) before and after removal of membrane HSPG by heparinase III. Removal of HSPG decreased the efficacy of infection with rAAV2 by only 30-35% at MOI < or = 100 for all of respiratory cell lines tested, and had even less effect at an MOI of 1000. Studies in mutant Chinese Hamster Ovary cell lines known to be completely deficient in surface HSPG also demonstrated only moderate effect of absence of HSPG on rAAV-2 infection efficacy. However, mutant CHO cells lacking all membrane proteoglycans demonstrated dramatic reduction in susceptibility to rAAV-2 infection, suggesting a role of membrane glycosaminoglycans other than HSPG in mediating rAAV-2 infection. CONCLUSION: Lack of cell membrane HSPG in pulmonary epithelia and other cell lines results in only moderate decrease in susceptibility to rAAV-2 infection, and this decrease may be less important at high MOIs. Other cell membrane glycosaminoglycans can play a role in permitting attachment and subsequent rAAV-2 internalization. Targeting alternative membrane glycosaminoglycans may aid in improving the efficacy of rAAV-2 for pulmonary applications. More... »

PAGES

29-29

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1743-422x-3-29

DOI

http://dx.doi.org/10.1186/1743-422x-3-29

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036064733

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16630361


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bronchi", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CHO Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chondroitin Sulfates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cricetinae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dependovirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Heparan Sulfate Proteoglycans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polysaccharide-Lyases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trachea", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA", 
          "id": "http://www.grid.ac/institutes/grid.21107.35", 
          "name": [
            "Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Boyle", 
        "givenName": "Michael P", 
        "id": "sg:person.0727074547.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727074547.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA", 
          "id": "http://www.grid.ac/institutes/grid.21107.35", 
          "name": [
            "Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Enke", 
        "givenName": "Raymond A", 
        "id": "sg:person.01035511146.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035511146.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA", 
          "id": "http://www.grid.ac/institutes/grid.21107.35", 
          "name": [
            "Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Reynolds", 
        "givenName": "Jeffrey B", 
        "id": "sg:person.0624532143.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624532143.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA", 
          "id": "http://www.grid.ac/institutes/grid.21107.35", 
          "name": [
            "Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mogayzel", 
        "givenName": "Peter J", 
        "id": "sg:person.01343027161.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343027161.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Physiology, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA", 
          "id": "http://www.grid.ac/institutes/grid.21107.35", 
          "name": [
            "Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA", 
            "Department of Physiology, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guggino", 
        "givenName": "William B", 
        "id": "sg:person.010645404217.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010645404217.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA", 
          "id": "http://www.grid.ac/institutes/grid.21107.35", 
          "name": [
            "Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zeitlin", 
        "givenName": "Pamela L", 
        "id": "sg:person.01335436266.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335436266.11"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/4758", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002093284", 
          "https://doi.org/10.1038/4758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/8526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016772957", 
          "https://doi.org/10.1038/8526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/4768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043860340", 
          "https://doi.org/10.1038/4768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053075732", 
          "https://doi.org/10.1038/sj.gt.3301109"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-04-22", 
    "datePublishedReg": "2006-04-22", 
    "description": "BACKGROUND: Adeno-associated virus type 2 (AAV-2) attachment and internalization is thought to be mediated by host cell membrane-associated heparan sulfate proteoglycans (HSPG). Lack of HSPG on the apical membrane of respiratory epithelial cells has been identified as a reason for inefficient rAAV-2 infection in pulmonary applications in-vivo. The aim of this investigation was to determine the necessity of cell membrane HSPG for efficient infection by rAAV-2.\nRESULTS: Rates of transduction with rAAV2-CMV-EGFP3 in several different immortalized airway epithelial cell lines were determined at different multiplicities of infection (MOI) before and after removal of membrane HSPG by heparinase III. Removal of HSPG decreased the efficacy of infection with rAAV2 by only 30-35% at MOI < or = 100 for all of respiratory cell lines tested, and had even less effect at an MOI of 1000. Studies in mutant Chinese Hamster Ovary cell lines known to be completely deficient in surface HSPG also demonstrated only moderate effect of absence of HSPG on rAAV-2 infection efficacy. However, mutant CHO cells lacking all membrane proteoglycans demonstrated dramatic reduction in susceptibility to rAAV-2 infection, suggesting a role of membrane glycosaminoglycans other than HSPG in mediating rAAV-2 infection.\nCONCLUSION: Lack of cell membrane HSPG in pulmonary epithelia and other cell lines results in only moderate decrease in susceptibility to rAAV-2 infection, and this decrease may be less important at high MOIs. Other cell membrane glycosaminoglycans can play a role in permitting attachment and subsequent rAAV-2 internalization. Targeting alternative membrane glycosaminoglycans may aid in improving the efficacy of rAAV-2 for pulmonary applications.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/1743-422x-3-29", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2436875", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1034449", 
        "issn": [
          "1743-422X"
        ], 
        "name": "Virology Journal", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "3"
      }
    ], 
    "keywords": [
      "cell lines", 
      "membrane glycosaminoglycans", 
      "respiratory cell lines", 
      "airway epithelial cell line", 
      "human respiratory epithelium", 
      "rAAV-2", 
      "respiratory epithelial cells", 
      "efficacy of infection", 
      "pulmonary application", 
      "epithelial cell line", 
      "respiratory epithelium", 
      "rate of transduction", 
      "pulmonary epithelium", 
      "infection efficacy", 
      "rAAV2-CMV", 
      "infection", 
      "only moderate decreases", 
      "epithelial cells", 
      "heparan sulfate proteoglycan", 
      "high MOI", 
      "efficient infection", 
      "efficacy", 
      "sulfate proteoglycan", 
      "moderate decrease", 
      "heparinase III", 
      "epithelium", 
      "heparan sulfate", 
      "membrane-associated heparan sulfate proteoglycan", 
      "hamster ovary cell line", 
      "Chinese hamster ovary cell line", 
      "moderate effect", 
      "ovary cell line", 
      "glycosaminoglycans", 
      "apical membrane", 
      "CHO cells", 
      "mutant CHO cells", 
      "cells", 
      "proteoglycans", 
      "dramatic reduction", 
      "less effect", 
      "MOI", 
      "mutant Chinese hamster ovary cell line", 
      "susceptibility", 
      "membrane proteoglycan", 
      "internalization", 
      "decrease", 
      "different multiplicities", 
      "vivo", 
      "role", 
      "adeno", 
      "rAAV2", 
      "lack", 
      "effect", 
      "aim", 
      "lines", 
      "membrane", 
      "absence", 
      "removal", 
      "study", 
      "rate", 
      "transduction", 
      "reduction", 
      "attachment", 
      "sulfate", 
      "reasons", 
      "investigation", 
      "necessity", 
      "multiplicity", 
      "applications", 
      "virus type 2 (AAV-2) attachment", 
      "type 2 (AAV-2) attachment", 
      "HSPG", 
      "cell membrane-associated heparan sulfate proteoglycans", 
      "Lack of HSPG", 
      "inefficient rAAV-2 infection", 
      "rAAV-2 infection", 
      "cell membrane HSPG", 
      "membrane HSPG", 
      "EGFP3", 
      "different immortalized airway epithelial cell lines", 
      "immortalized airway epithelial cell lines", 
      "Removal of HSPG", 
      "surface HSPG", 
      "rAAV-2 infection efficacy", 
      "cell membrane glycosaminoglycans", 
      "subsequent rAAV-2 internalization", 
      "rAAV-2 internalization", 
      "alternative membrane glycosaminoglycans"
    ], 
    "name": "Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia", 
    "pagination": "29-29", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036064733"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1743-422x-3-29"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16630361"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1743-422x-3-29", 
      "https://app.dimensions.ai/details/publication/pub.1036064733"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:15", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_416.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/1743-422x-3-29"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-29'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-29'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-29'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-29'


 

This table displays all metadata directly associated to this object as RDF triples.

254 TRIPLES      22 PREDICATES      130 URIs      118 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1743-422x-3-29 schema:about N0f4633d8ec0d447bbccebbc3faf2dd73
2 N2c696ab2b15b436ebc193fc3b8f9845a
3 N482829d7b65f4e51966201ef628719e2
4 N49047168a3a4406798782c173b24f824
5 N515c37825e4d4f48bd605fdaae64e397
6 N80779817e365439ebf3c946147303799
7 N80c2c2d25cfd44889a2abcb5bfe9b8e1
8 N9324fabcc9014ab897939a816c344e52
9 Nc27d11b130da47318e70164e51fc060c
10 Nc50a5310fccc488983f84bb7ecb1112e
11 Ndc676ab46f7545b7936db14e4f095a38
12 Nfc7e6ac0fe5f4eb0a7a083530381f0c9
13 anzsrc-for:11
14 anzsrc-for:1108
15 schema:author Nf7a624664c394abe9d28183596cf97e8
16 schema:citation sg:pub.10.1038/4758
17 sg:pub.10.1038/4768
18 sg:pub.10.1038/8526
19 sg:pub.10.1038/sj.gt.3301109
20 schema:datePublished 2006-04-22
21 schema:datePublishedReg 2006-04-22
22 schema:description BACKGROUND: Adeno-associated virus type 2 (AAV-2) attachment and internalization is thought to be mediated by host cell membrane-associated heparan sulfate proteoglycans (HSPG). Lack of HSPG on the apical membrane of respiratory epithelial cells has been identified as a reason for inefficient rAAV-2 infection in pulmonary applications in-vivo. The aim of this investigation was to determine the necessity of cell membrane HSPG for efficient infection by rAAV-2. RESULTS: Rates of transduction with rAAV2-CMV-EGFP3 in several different immortalized airway epithelial cell lines were determined at different multiplicities of infection (MOI) before and after removal of membrane HSPG by heparinase III. Removal of HSPG decreased the efficacy of infection with rAAV2 by only 30-35% at MOI < or = 100 for all of respiratory cell lines tested, and had even less effect at an MOI of 1000. Studies in mutant Chinese Hamster Ovary cell lines known to be completely deficient in surface HSPG also demonstrated only moderate effect of absence of HSPG on rAAV-2 infection efficacy. However, mutant CHO cells lacking all membrane proteoglycans demonstrated dramatic reduction in susceptibility to rAAV-2 infection, suggesting a role of membrane glycosaminoglycans other than HSPG in mediating rAAV-2 infection. CONCLUSION: Lack of cell membrane HSPG in pulmonary epithelia and other cell lines results in only moderate decrease in susceptibility to rAAV-2 infection, and this decrease may be less important at high MOIs. Other cell membrane glycosaminoglycans can play a role in permitting attachment and subsequent rAAV-2 internalization. Targeting alternative membrane glycosaminoglycans may aid in improving the efficacy of rAAV-2 for pulmonary applications.
23 schema:genre article
24 schema:inLanguage en
25 schema:isAccessibleForFree true
26 schema:isPartOf Nb7b3fadb428f4a3ea59c75b2855e4f61
27 Nfbd5b10e01ee4b51b0b77efb5a100a91
28 sg:journal.1034449
29 schema:keywords CHO cells
30 Chinese hamster ovary cell line
31 EGFP3
32 HSPG
33 Lack of HSPG
34 MOI
35 Removal of HSPG
36 absence
37 adeno
38 aim
39 airway epithelial cell line
40 alternative membrane glycosaminoglycans
41 apical membrane
42 applications
43 attachment
44 cell lines
45 cell membrane HSPG
46 cell membrane glycosaminoglycans
47 cell membrane-associated heparan sulfate proteoglycans
48 cells
49 decrease
50 different immortalized airway epithelial cell lines
51 different multiplicities
52 dramatic reduction
53 effect
54 efficacy
55 efficacy of infection
56 efficient infection
57 epithelial cell line
58 epithelial cells
59 epithelium
60 glycosaminoglycans
61 hamster ovary cell line
62 heparan sulfate
63 heparan sulfate proteoglycan
64 heparinase III
65 high MOI
66 human respiratory epithelium
67 immortalized airway epithelial cell lines
68 inefficient rAAV-2 infection
69 infection
70 infection efficacy
71 internalization
72 investigation
73 lack
74 less effect
75 lines
76 membrane
77 membrane HSPG
78 membrane glycosaminoglycans
79 membrane proteoglycan
80 membrane-associated heparan sulfate proteoglycan
81 moderate decrease
82 moderate effect
83 multiplicity
84 mutant CHO cells
85 mutant Chinese hamster ovary cell line
86 necessity
87 only moderate decreases
88 ovary cell line
89 proteoglycans
90 pulmonary application
91 pulmonary epithelium
92 rAAV-2
93 rAAV-2 infection
94 rAAV-2 infection efficacy
95 rAAV-2 internalization
96 rAAV2
97 rAAV2-CMV
98 rate
99 rate of transduction
100 reasons
101 reduction
102 removal
103 respiratory cell lines
104 respiratory epithelial cells
105 respiratory epithelium
106 role
107 study
108 subsequent rAAV-2 internalization
109 sulfate
110 sulfate proteoglycan
111 surface HSPG
112 susceptibility
113 transduction
114 type 2 (AAV-2) attachment
115 virus type 2 (AAV-2) attachment
116 vivo
117 schema:name Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia
118 schema:pagination 29-29
119 schema:productId N1d14b1f58066495e9614c919ac376ac5
120 Na91f43485dac48b5a0276969444cbf7c
121 Ne1b2198ce7bb4b2bbe8cb017d609f0a7
122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036064733
123 https://doi.org/10.1186/1743-422x-3-29
124 schema:sdDatePublished 2022-01-01T18:15
125 schema:sdLicense https://scigraph.springernature.com/explorer/license/
126 schema:sdPublisher N389c38cb1f144ad895aa49fd9a2071f5
127 schema:url https://doi.org/10.1186/1743-422x-3-29
128 sgo:license sg:explorer/license/
129 sgo:sdDataset articles
130 rdf:type schema:ScholarlyArticle
131 N0f4633d8ec0d447bbccebbc3faf2dd73 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Dependovirus
133 rdf:type schema:DefinedTerm
134 N16e704d4b1074b988def07a86a9a2436 rdf:first sg:person.01035511146.16
135 rdf:rest N8af2fcef8a9c4a34bef82a856853b4f2
136 N1d14b1f58066495e9614c919ac376ac5 schema:name doi
137 schema:value 10.1186/1743-422x-3-29
138 rdf:type schema:PropertyValue
139 N2601094b1b924a12acaf61c6f678e41d rdf:first sg:person.010645404217.56
140 rdf:rest N413193718f1f452ea132e7f406e4aee0
141 N2c696ab2b15b436ebc193fc3b8f9845a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Cell Line
143 rdf:type schema:DefinedTerm
144 N389c38cb1f144ad895aa49fd9a2071f5 schema:name Springer Nature - SN SciGraph project
145 rdf:type schema:Organization
146 N413193718f1f452ea132e7f406e4aee0 rdf:first sg:person.01335436266.11
147 rdf:rest rdf:nil
148 N482829d7b65f4e51966201ef628719e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Chondroitin Sulfates
150 rdf:type schema:DefinedTerm
151 N49047168a3a4406798782c173b24f824 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Membrane Proteins
153 rdf:type schema:DefinedTerm
154 N496f372810ac45d8b4b170a1ea3d4123 rdf:first sg:person.01343027161.45
155 rdf:rest N2601094b1b924a12acaf61c6f678e41d
156 N515c37825e4d4f48bd605fdaae64e397 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name CHO Cells
158 rdf:type schema:DefinedTerm
159 N80779817e365439ebf3c946147303799 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Humans
161 rdf:type schema:DefinedTerm
162 N80c2c2d25cfd44889a2abcb5bfe9b8e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Animals
164 rdf:type schema:DefinedTerm
165 N8af2fcef8a9c4a34bef82a856853b4f2 rdf:first sg:person.0624532143.70
166 rdf:rest N496f372810ac45d8b4b170a1ea3d4123
167 N9324fabcc9014ab897939a816c344e52 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Cricetinae
169 rdf:type schema:DefinedTerm
170 Na91f43485dac48b5a0276969444cbf7c schema:name pubmed_id
171 schema:value 16630361
172 rdf:type schema:PropertyValue
173 Nb7b3fadb428f4a3ea59c75b2855e4f61 schema:issueNumber 1
174 rdf:type schema:PublicationIssue
175 Nc27d11b130da47318e70164e51fc060c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Polysaccharide-Lyases
177 rdf:type schema:DefinedTerm
178 Nc50a5310fccc488983f84bb7ecb1112e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Trachea
180 rdf:type schema:DefinedTerm
181 Ndc676ab46f7545b7936db14e4f095a38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Heparan Sulfate Proteoglycans
183 rdf:type schema:DefinedTerm
184 Ne1b2198ce7bb4b2bbe8cb017d609f0a7 schema:name dimensions_id
185 schema:value pub.1036064733
186 rdf:type schema:PropertyValue
187 Nf7a624664c394abe9d28183596cf97e8 rdf:first sg:person.0727074547.04
188 rdf:rest N16e704d4b1074b988def07a86a9a2436
189 Nfbd5b10e01ee4b51b0b77efb5a100a91 schema:volumeNumber 3
190 rdf:type schema:PublicationVolume
191 Nfc7e6ac0fe5f4eb0a7a083530381f0c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Bronchi
193 rdf:type schema:DefinedTerm
194 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
195 schema:name Medical and Health Sciences
196 rdf:type schema:DefinedTerm
197 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
198 schema:name Medical Microbiology
199 rdf:type schema:DefinedTerm
200 sg:grant.2436875 http://pending.schema.org/fundedItem sg:pub.10.1186/1743-422x-3-29
201 rdf:type schema:MonetaryGrant
202 sg:journal.1034449 schema:issn 1743-422X
203 schema:name Virology Journal
204 schema:publisher Springer Nature
205 rdf:type schema:Periodical
206 sg:person.01035511146.16 schema:affiliation grid-institutes:grid.21107.35
207 schema:familyName Enke
208 schema:givenName Raymond A
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035511146.16
210 rdf:type schema:Person
211 sg:person.010645404217.56 schema:affiliation grid-institutes:grid.21107.35
212 schema:familyName Guggino
213 schema:givenName William B
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010645404217.56
215 rdf:type schema:Person
216 sg:person.01335436266.11 schema:affiliation grid-institutes:grid.21107.35
217 schema:familyName Zeitlin
218 schema:givenName Pamela L
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335436266.11
220 rdf:type schema:Person
221 sg:person.01343027161.45 schema:affiliation grid-institutes:grid.21107.35
222 schema:familyName Mogayzel
223 schema:givenName Peter J
224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343027161.45
225 rdf:type schema:Person
226 sg:person.0624532143.70 schema:affiliation grid-institutes:grid.21107.35
227 schema:familyName Reynolds
228 schema:givenName Jeffrey B
229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624532143.70
230 rdf:type schema:Person
231 sg:person.0727074547.04 schema:affiliation grid-institutes:grid.21107.35
232 schema:familyName Boyle
233 schema:givenName Michael P
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727074547.04
235 rdf:type schema:Person
236 sg:pub.10.1038/4758 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002093284
237 https://doi.org/10.1038/4758
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/4768 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043860340
240 https://doi.org/10.1038/4768
241 rdf:type schema:CreativeWork
242 sg:pub.10.1038/8526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016772957
243 https://doi.org/10.1038/8526
244 rdf:type schema:CreativeWork
245 sg:pub.10.1038/sj.gt.3301109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053075732
246 https://doi.org/10.1038/sj.gt.3301109
247 rdf:type schema:CreativeWork
248 grid-institutes:grid.21107.35 schema:alternateName Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA
249 Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA
250 Department of Physiology, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA
251 schema:name Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA
252 Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA
253 Department of Physiology, The Johns Hopkins University School of Medicine, Baltimore MD 21205, USA
254 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...