Mutagenesis of tyrosine and di-leucine motifs in the HIV-1 envelope cytoplasmic domain results in a loss of Env-mediated fusion and ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-05-14

AUTHORS

Sushma J Bhakta, Liang Shang, Jessica L Prince, Daniel T Claiborne, Eric Hunter

ABSTRACT

BACKGROUND: The gp41 component of the Human Immunodeficiency Virus (HIV) envelope glycoprotein (Env) contains a long cytoplasmic domain (CD) with multiple highly conserved tyrosine (Y) and dileucine (LL) motifs. Studies suggest that the motifs distal to major endocytosis motif (Y712HRL), located at residues 712-715 of Env, may contribute to Env functionality in the viral life cycle. In order to examine the biological contribution of these motifs in the biosynthesis, transport, and function of Env, we constructed two panels of mutants in which the conserved Y- and LL-motifs were sequentially substituted by alternative residues, either in the presence or absence of Y712. Additional mutants targeting individual motifs were then constructed. RESULTS: All mutant Envs, when expressed in the absence of other viral proteins, maintained at least WT levels of Env surface staining by multiple antibodies. The Y712 mutation (Y712C) contributed to at least a 4-fold increase in surface expression for all mutants containing this change. Sequential mutagenesis of the Y- and LL-motifs resulted in a generally progressive decrease in Env fusogenicity. However, additive mutation of dileucine and tyrosine motifs beyond the tyrosine at residue 768 resulted in the most dramatic effects on Env incorporation into virions, viral infectivity, and virus fusion with target cells. CONCLUSIONS: From the studies reported here, we show that mutations of the Y- and LL-motifs, which effectively eliminate the amphipathic nature of the lytic peptide 2 (LLP2) domain or disrupt YW and LL motifs in a region spanning residues 795-803 (YWWNLLQYW), just C-terminal of LLP2, can dramatically interfere with biological functions of HIV-1 Env and abrogate virus replication. Because these mutant proteins are expressed at the cell surface, we conclude that tyrosine and di-leucine residues within the cytoplasmic domain of gp41 play critical roles in HIV-1 replication that are distinct from that of targeting the plasma membrane. More... »

PAGES

37-37

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1742-4690-8-37

DOI

http://dx.doi.org/10.1186/1742-4690-8-37

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028880841

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21569545


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Motifs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Substitution", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chlorocebus aethiops", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Mutational Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HIV-1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leucine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis, Site-Directed", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation, Missense", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tyrosine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viral Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virulence Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Internalization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "env Gene Products, Human Immunodeficiency Virus", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA", 
          "id": "http://www.grid.ac/institutes/grid.189967.8", 
          "name": [
            "Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bhakta", 
        "givenName": "Sushma J", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA", 
          "id": "http://www.grid.ac/institutes/grid.189967.8", 
          "name": [
            "Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shang", 
        "givenName": "Liang", 
        "id": "sg:person.01115202713.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115202713.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA", 
          "id": "http://www.grid.ac/institutes/grid.189967.8", 
          "name": [
            "Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Prince", 
        "givenName": "Jessica L", 
        "id": "sg:person.0652070552.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652070552.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA", 
          "id": "http://www.grid.ac/institutes/grid.189967.8", 
          "name": [
            "Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Claiborne", 
        "givenName": "Daniel T", 
        "id": "sg:person.01047475567.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047475567.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA", 
          "id": "http://www.grid.ac/institutes/grid.189967.8", 
          "name": [
            "Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hunter", 
        "givenName": "Eric", 
        "id": "sg:person.0731773046.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731773046.09"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/978-3-642-75218-6_7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033789163", 
          "https://doi.org/10.1007/978-3-642-75218-6_7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt745", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018667257", 
          "https://doi.org/10.1038/nbt745"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature06998", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037721396", 
          "https://doi.org/10.1038/nature06998"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-79657-9_14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006960547", 
          "https://doi.org/10.1007/978-3-642-79657-9_14"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-05-14", 
    "datePublishedReg": "2011-05-14", 
    "description": "BACKGROUND: The gp41 component of the Human Immunodeficiency Virus (HIV) envelope glycoprotein (Env) contains a long cytoplasmic domain (CD) with multiple highly conserved tyrosine (Y) and dileucine (LL) motifs. Studies suggest that the motifs distal to major endocytosis motif (Y712HRL), located at residues 712-715 of Env, may contribute to Env functionality in the viral life cycle. In order to examine the biological contribution of these motifs in the biosynthesis, transport, and function of Env, we constructed two panels of mutants in which the conserved Y- and LL-motifs were sequentially substituted by alternative residues, either in the presence or absence of Y712. Additional mutants targeting individual motifs were then constructed.\nRESULTS: All mutant Envs, when expressed in the absence of other viral proteins, maintained at least WT levels of Env surface staining by multiple antibodies. The Y712 mutation (Y712C) contributed to at least a 4-fold increase in surface expression for all mutants containing this change. Sequential mutagenesis of the Y- and LL-motifs resulted in a generally progressive decrease in Env fusogenicity. However, additive mutation of dileucine and tyrosine motifs beyond the tyrosine at residue 768 resulted in the most dramatic effects on Env incorporation into virions, viral infectivity, and virus fusion with target cells.\nCONCLUSIONS: From the studies reported here, we show that mutations of the Y- and LL-motifs, which effectively eliminate the amphipathic nature of the lytic peptide 2 (LLP2) domain or disrupt YW and LL motifs in a region spanning residues 795-803 (YWWNLLQYW), just C-terminal of LLP2, can dramatically interfere with biological functions of HIV-1 Env and abrogate virus replication. Because these mutant proteins are expressed at the cell surface, we conclude that tyrosine and di-leucine residues within the cytoplasmic domain of gp41 play critical roles in HIV-1 replication that are distinct from that of targeting the plasma membrane.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/1742-4690-8-37", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2438706", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2452575", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1033800", 
        "issn": [
          "1742-4690"
        ], 
        "name": "Retrovirology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "keywords": [
      "function of Env", 
      "human immunodeficiency virus envelope glycoprotein", 
      "HIV-1 replication", 
      "HIV-1 Env", 
      "Env-mediated fusion", 
      "viral life cycle", 
      "virus envelope glycoprotein", 
      "virus replication", 
      "multiple antibodies", 
      "viral infectivity", 
      "envelope glycoprotein", 
      "Env surface", 
      "target cells", 
      "Env incorporation", 
      "Env", 
      "surface expression", 
      "Env fusogenicity", 
      "progressive decrease", 
      "long cytoplasmic domain", 
      "mutant Env", 
      "viral proteins", 
      "WT levels", 
      "cytoplasmic domain", 
      "virus fusion", 
      "infectivity", 
      "critical role", 
      "cell surface", 
      "mutations", 
      "LL motif", 
      "antibodies", 
      "biological functions", 
      "tyrosine", 
      "gp41", 
      "absence", 
      "replication", 
      "study", 
      "protein", 
      "panel of mutants", 
      "additive mutations", 
      "di-leucine motif", 
      "plasma membrane", 
      "glycoprotein", 
      "cells", 
      "virions", 
      "biological contributions", 
      "expression", 
      "tyrosine motif", 
      "dileucine motif", 
      "region spanning", 
      "endocytosis motif", 
      "dramatic effect", 
      "function", 
      "levels", 
      "decrease", 
      "fusion", 
      "role", 
      "increase", 
      "mutant proteins", 
      "fusogenicity", 
      "loss", 
      "effect", 
      "panel", 
      "terminals", 
      "presence", 
      "LLP2", 
      "additional mutants", 
      "changes", 
      "mutants", 
      "alternative residues", 
      "amphipathic nature", 
      "membrane", 
      "mutagenesis", 
      "life cycle", 
      "biosynthesis", 
      "domain", 
      "cycle", 
      "incorporation", 
      "motif", 
      "components", 
      "residues", 
      "dileucine", 
      "YW", 
      "transport", 
      "contribution", 
      "nature", 
      "sequential mutagenesis", 
      "order", 
      "surface", 
      "spanning", 
      "individual motives", 
      "functionality", 
      "gp41 component", 
      "Immunodeficiency Virus (HIV) envelope glycoprotein", 
      "major endocytosis motif", 
      "residues 712", 
      "Env functionality", 
      "absence of Y712", 
      "Y712", 
      "least WT levels", 
      "Y712 mutation", 
      "residue 768", 
      "lytic peptide 2 (LLP2) domain", 
      "peptide 2 (LLP2) domain", 
      "terminal of LLP2", 
      "di-leucine residues", 
      "Mutagenesis of tyrosine", 
      "HIV-1 envelope cytoplasmic domain", 
      "envelope cytoplasmic domain"
    ], 
    "name": "Mutagenesis of tyrosine and di-leucine motifs in the HIV-1 envelope cytoplasmic domain results in a loss of Env-mediated fusion and infectivity", 
    "pagination": "37-37", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1028880841"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1742-4690-8-37"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21569545"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1742-4690-8-37", 
      "https://app.dimensions.ai/details/publication/pub.1028880841"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:24", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_530.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/1742-4690-8-37"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1742-4690-8-37'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1742-4690-8-37'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1742-4690-8-37'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1742-4690-8-37'


 

This table displays all metadata directly associated to this object as RDF triples.

288 TRIPLES      22 PREDICATES      156 URIs      144 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1742-4690-8-37 schema:about N01c81194da22495cb3a2e9f3608c417c
2 N1068d25dcb07452cac7291122ca2c56d
3 N14095d71c39e4802a984ba7f7e556f3d
4 N2cc2c82d081748bca9dc4e72206b04bc
5 N4108bad06e2a45e1b3ca91cda5372854
6 N4a34c68905934d8193c60ed5b9b89c7e
7 N549207976b644b748d19874e44cb519e
8 N6036341bcc2747efb8080b93d0606790
9 N608719c33fbc43caa2d9677510733fb5
10 N711a7fb1b7f048588a4ef7bec7b580f8
11 N8763891cc1224ce3be93b268079bae0f
12 N96df086921e04da79e51d239464e0882
13 N9c9082f32ead4aa5bd889870db08b493
14 N9dd87c5190874df2aa096d5bd4646de9
15 Na185e2a1d9ec44ff86df9b0ab920d9bf
16 Ne9ece415b7684c448f23158a1b54871a
17 Nfec05a841ea744048c419920fb2f427c
18 Nffaf3236bcee4857b85ae506aecdb444
19 anzsrc-for:11
20 anzsrc-for:1108
21 schema:author N0ecad0d03a804d0f8c329343410437a2
22 schema:citation sg:pub.10.1007/978-3-642-75218-6_7
23 sg:pub.10.1007/978-3-642-79657-9_14
24 sg:pub.10.1038/nature06998
25 sg:pub.10.1038/nbt745
26 schema:datePublished 2011-05-14
27 schema:datePublishedReg 2011-05-14
28 schema:description BACKGROUND: The gp41 component of the Human Immunodeficiency Virus (HIV) envelope glycoprotein (Env) contains a long cytoplasmic domain (CD) with multiple highly conserved tyrosine (Y) and dileucine (LL) motifs. Studies suggest that the motifs distal to major endocytosis motif (Y712HRL), located at residues 712-715 of Env, may contribute to Env functionality in the viral life cycle. In order to examine the biological contribution of these motifs in the biosynthesis, transport, and function of Env, we constructed two panels of mutants in which the conserved Y- and LL-motifs were sequentially substituted by alternative residues, either in the presence or absence of Y712. Additional mutants targeting individual motifs were then constructed. RESULTS: All mutant Envs, when expressed in the absence of other viral proteins, maintained at least WT levels of Env surface staining by multiple antibodies. The Y712 mutation (Y712C) contributed to at least a 4-fold increase in surface expression for all mutants containing this change. Sequential mutagenesis of the Y- and LL-motifs resulted in a generally progressive decrease in Env fusogenicity. However, additive mutation of dileucine and tyrosine motifs beyond the tyrosine at residue 768 resulted in the most dramatic effects on Env incorporation into virions, viral infectivity, and virus fusion with target cells. CONCLUSIONS: From the studies reported here, we show that mutations of the Y- and LL-motifs, which effectively eliminate the amphipathic nature of the lytic peptide 2 (LLP2) domain or disrupt YW and LL motifs in a region spanning residues 795-803 (YWWNLLQYW), just C-terminal of LLP2, can dramatically interfere with biological functions of HIV-1 Env and abrogate virus replication. Because these mutant proteins are expressed at the cell surface, we conclude that tyrosine and di-leucine residues within the cytoplasmic domain of gp41 play critical roles in HIV-1 replication that are distinct from that of targeting the plasma membrane.
29 schema:genre article
30 schema:inLanguage en
31 schema:isAccessibleForFree true
32 schema:isPartOf Nb11281983d3d4cdaacefe9bae40bf10e
33 Nc177871736614377938d76fe2688f721
34 sg:journal.1033800
35 schema:keywords Env
36 Env functionality
37 Env fusogenicity
38 Env incorporation
39 Env surface
40 Env-mediated fusion
41 HIV-1 Env
42 HIV-1 envelope cytoplasmic domain
43 HIV-1 replication
44 Immunodeficiency Virus (HIV) envelope glycoprotein
45 LL motif
46 LLP2
47 Mutagenesis of tyrosine
48 WT levels
49 Y712
50 Y712 mutation
51 YW
52 absence
53 absence of Y712
54 additional mutants
55 additive mutations
56 alternative residues
57 amphipathic nature
58 antibodies
59 biological contributions
60 biological functions
61 biosynthesis
62 cell surface
63 cells
64 changes
65 components
66 contribution
67 critical role
68 cycle
69 cytoplasmic domain
70 decrease
71 di-leucine motif
72 di-leucine residues
73 dileucine
74 dileucine motif
75 domain
76 dramatic effect
77 effect
78 endocytosis motif
79 envelope cytoplasmic domain
80 envelope glycoprotein
81 expression
82 function
83 function of Env
84 functionality
85 fusion
86 fusogenicity
87 glycoprotein
88 gp41
89 gp41 component
90 human immunodeficiency virus envelope glycoprotein
91 incorporation
92 increase
93 individual motives
94 infectivity
95 least WT levels
96 levels
97 life cycle
98 long cytoplasmic domain
99 loss
100 lytic peptide 2 (LLP2) domain
101 major endocytosis motif
102 membrane
103 motif
104 multiple antibodies
105 mutagenesis
106 mutant Env
107 mutant proteins
108 mutants
109 mutations
110 nature
111 order
112 panel
113 panel of mutants
114 peptide 2 (LLP2) domain
115 plasma membrane
116 presence
117 progressive decrease
118 protein
119 region spanning
120 replication
121 residue 768
122 residues
123 residues 712
124 role
125 sequential mutagenesis
126 spanning
127 study
128 surface
129 surface expression
130 target cells
131 terminal of LLP2
132 terminals
133 transport
134 tyrosine
135 tyrosine motif
136 viral infectivity
137 viral life cycle
138 viral proteins
139 virions
140 virus envelope glycoprotein
141 virus fusion
142 virus replication
143 schema:name Mutagenesis of tyrosine and di-leucine motifs in the HIV-1 envelope cytoplasmic domain results in a loss of Env-mediated fusion and infectivity
144 schema:pagination 37-37
145 schema:productId N17740045b1bf4ccd8deb715d755e4859
146 N580fccd8d7dc4d64afa1fb95131ac4f5
147 Nc6dcf950a0a24e74bd8ee6385556039e
148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028880841
149 https://doi.org/10.1186/1742-4690-8-37
150 schema:sdDatePublished 2021-12-01T19:24
151 schema:sdLicense https://scigraph.springernature.com/explorer/license/
152 schema:sdPublisher N6557d4fe0a794e8d95f42892e1bd7e98
153 schema:url https://doi.org/10.1186/1742-4690-8-37
154 sgo:license sg:explorer/license/
155 sgo:sdDataset articles
156 rdf:type schema:ScholarlyArticle
157 N01c81194da22495cb3a2e9f3608c417c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Mutation, Missense
159 rdf:type schema:DefinedTerm
160 N0ecad0d03a804d0f8c329343410437a2 rdf:first N349d5eaf62dc43c4ae8174c5e3f243df
161 rdf:rest N606f895f621246cf9110e9bd4fdbc6f6
162 N1068d25dcb07452cac7291122ca2c56d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Molecular Sequence Data
164 rdf:type schema:DefinedTerm
165 N14095d71c39e4802a984ba7f7e556f3d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Tyrosine
167 rdf:type schema:DefinedTerm
168 N17740045b1bf4ccd8deb715d755e4859 schema:name dimensions_id
169 schema:value pub.1028880841
170 rdf:type schema:PropertyValue
171 N2cc2c82d081748bca9dc4e72206b04bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name HIV-1
173 rdf:type schema:DefinedTerm
174 N30877fecf7e6414fa31e3fa155c17cc3 rdf:first sg:person.0652070552.20
175 rdf:rest Nd928bc091b374469b9c55018749d05bc
176 N349d5eaf62dc43c4ae8174c5e3f243df schema:affiliation grid-institutes:grid.189967.8
177 schema:familyName Bhakta
178 schema:givenName Sushma J
179 rdf:type schema:Person
180 N4108bad06e2a45e1b3ca91cda5372854 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Virulence Factors
182 rdf:type schema:DefinedTerm
183 N4a34c68905934d8193c60ed5b9b89c7e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Amino Acid Motifs
185 rdf:type schema:DefinedTerm
186 N549207976b644b748d19874e44cb519e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name Animals
188 rdf:type schema:DefinedTerm
189 N580fccd8d7dc4d64afa1fb95131ac4f5 schema:name doi
190 schema:value 10.1186/1742-4690-8-37
191 rdf:type schema:PropertyValue
192 N6036341bcc2747efb8080b93d0606790 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
193 schema:name Amino Acid Sequence
194 rdf:type schema:DefinedTerm
195 N606f895f621246cf9110e9bd4fdbc6f6 rdf:first sg:person.01115202713.29
196 rdf:rest N30877fecf7e6414fa31e3fa155c17cc3
197 N608719c33fbc43caa2d9677510733fb5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name Virus Internalization
199 rdf:type schema:DefinedTerm
200 N6557d4fe0a794e8d95f42892e1bd7e98 schema:name Springer Nature - SN SciGraph project
201 rdf:type schema:Organization
202 N711a7fb1b7f048588a4ef7bec7b580f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
203 schema:name env Gene Products, Human Immunodeficiency Virus
204 rdf:type schema:DefinedTerm
205 N8763891cc1224ce3be93b268079bae0f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
206 schema:name Viral Fusion Proteins
207 rdf:type schema:DefinedTerm
208 N96df086921e04da79e51d239464e0882 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
209 schema:name Amino Acid Substitution
210 rdf:type schema:DefinedTerm
211 N9c9082f32ead4aa5bd889870db08b493 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
212 schema:name Leucine
213 rdf:type schema:DefinedTerm
214 N9dd87c5190874df2aa096d5bd4646de9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
215 schema:name DNA Mutational Analysis
216 rdf:type schema:DefinedTerm
217 Na185e2a1d9ec44ff86df9b0ab920d9bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
218 schema:name Mutagenesis, Site-Directed
219 rdf:type schema:DefinedTerm
220 Nb11281983d3d4cdaacefe9bae40bf10e schema:issueNumber 1
221 rdf:type schema:PublicationIssue
222 Nc04ab392cf5441d7a1c218bd18da1463 rdf:first sg:person.0731773046.09
223 rdf:rest rdf:nil
224 Nc177871736614377938d76fe2688f721 schema:volumeNumber 8
225 rdf:type schema:PublicationVolume
226 Nc6dcf950a0a24e74bd8ee6385556039e schema:name pubmed_id
227 schema:value 21569545
228 rdf:type schema:PropertyValue
229 Nd928bc091b374469b9c55018749d05bc rdf:first sg:person.01047475567.62
230 rdf:rest Nc04ab392cf5441d7a1c218bd18da1463
231 Ne9ece415b7684c448f23158a1b54871a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
232 schema:name Humans
233 rdf:type schema:DefinedTerm
234 Nfec05a841ea744048c419920fb2f427c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
235 schema:name Cell Line
236 rdf:type schema:DefinedTerm
237 Nffaf3236bcee4857b85ae506aecdb444 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
238 schema:name Chlorocebus aethiops
239 rdf:type schema:DefinedTerm
240 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
241 schema:name Medical and Health Sciences
242 rdf:type schema:DefinedTerm
243 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
244 schema:name Medical Microbiology
245 rdf:type schema:DefinedTerm
246 sg:grant.2438706 http://pending.schema.org/fundedItem sg:pub.10.1186/1742-4690-8-37
247 rdf:type schema:MonetaryGrant
248 sg:grant.2452575 http://pending.schema.org/fundedItem sg:pub.10.1186/1742-4690-8-37
249 rdf:type schema:MonetaryGrant
250 sg:journal.1033800 schema:issn 1742-4690
251 schema:name Retrovirology
252 schema:publisher Springer Nature
253 rdf:type schema:Periodical
254 sg:person.01047475567.62 schema:affiliation grid-institutes:grid.189967.8
255 schema:familyName Claiborne
256 schema:givenName Daniel T
257 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047475567.62
258 rdf:type schema:Person
259 sg:person.01115202713.29 schema:affiliation grid-institutes:grid.189967.8
260 schema:familyName Shang
261 schema:givenName Liang
262 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115202713.29
263 rdf:type schema:Person
264 sg:person.0652070552.20 schema:affiliation grid-institutes:grid.189967.8
265 schema:familyName Prince
266 schema:givenName Jessica L
267 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652070552.20
268 rdf:type schema:Person
269 sg:person.0731773046.09 schema:affiliation grid-institutes:grid.189967.8
270 schema:familyName Hunter
271 schema:givenName Eric
272 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731773046.09
273 rdf:type schema:Person
274 sg:pub.10.1007/978-3-642-75218-6_7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033789163
275 https://doi.org/10.1007/978-3-642-75218-6_7
276 rdf:type schema:CreativeWork
277 sg:pub.10.1007/978-3-642-79657-9_14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006960547
278 https://doi.org/10.1007/978-3-642-79657-9_14
279 rdf:type schema:CreativeWork
280 sg:pub.10.1038/nature06998 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037721396
281 https://doi.org/10.1038/nature06998
282 rdf:type schema:CreativeWork
283 sg:pub.10.1038/nbt745 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018667257
284 https://doi.org/10.1038/nbt745
285 rdf:type schema:CreativeWork
286 grid-institutes:grid.189967.8 schema:alternateName Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA
287 schema:name Emory Vaccine Center at the Yerkes National Primate Research Center and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia 30329, USA
288 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...