L-leucine, beta-hydroxy-beta-methylbutyric acid (HMB) and creatine monohydrate prevent myostatin-induced Akirin-1/Mighty mRNA down-regulation and myotube atrophy View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-08-13

AUTHORS

Christopher Brooks Mobley, Carlton D Fox, Brian S Ferguson, Rajesh H Amin, Vincent J Dalbo, Shawn Baier, John A Rathmacher, Jacob M Wilson, Michael D Roberts

ABSTRACT

BACKGROUND: The purpose of this study was to examine if L-leucine (Leu), β-hydroxy-β-methylbutyrate (HMB), or creatine monohydrate (Crea) prevented potential atrophic effects of myostatin (MSTN) on differentiated C2C12 myotubes. METHODS: After four days of differentiation, myotubes were treated with MSTN (10 ng/ml) for two additional days and four treatment groups were studied: 1) 3x per day 10 mM Leu, 2) 3x per day 10 mM HMB, 3) 3x per day 10 mM Crea, 4) DM only. Myotubes treated with DM without MSTN were analyzed as the control condition (DM/CTL). Following treatment, cells were analyzed for total protein, DNA content, RNA content, muscle protein synthesis (MPS, SUnSET method), and fiber diameter. Separate batch treatments were analyzed for mRNA expression patterns of myostatin-related genes (Akirin-1/Mighty, Notch-1, Ski, MyoD) as well as atrogenes (MuRF-1, and MAFbx/Atrogin-1). RESULTS: MSTN decreased fiber diameter approximately 30% compared to DM/CTL myotubes (p < 0.001). Leu, HMB and Crea prevented MSTN-induced atrophy. MSTN did not decrease MPS levels compared to DM/CTL myotubes, but MSTN treatment decreased the mRNA expression of Akirin-1/Mighty by 27% (p < 0.001) and MyoD by 26% (p < 0.01) compared to DM/CTL myotubes. shRNA experiments confirmed that Mighty mRNA knockdown reduced myotube size, linking MSTN treatment to atrophy independent of MPS. Remarkably, MSTN + Leu and MSTN + HMB myotubes had similar Akirin-1/Mighty and MyoD mRNA levels compared to DM/CTL myotubes. Furthermore, MSTN + Crea myotubes exhibited a 36% (p < 0.05) and 86% (p < 0.001) increase in Akirin-1/Mighty mRNA compared to DM/CTL and MSTN-only treated myotubes, respectively. CONCLUSIONS: Leu, HMB and Crea may reduce MSTN-induced muscle fiber atrophy by influencing Akirin-1/Mighty mRNA expression patterns. Future studies are needed to examine if Leu, HMB and Crea independently or synergistically affect Akirin-1/Mighty expression, and how Akirin-1/Mighty expression mechanistically relates to skeletal muscle hypertrophy in vivo. More... »

PAGES

38-38

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1550-2783-11-38

DOI

http://dx.doi.org/10.1186/1550-2783-11-38

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001591525

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25132809


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849, USA", 
          "id": "http://www.grid.ac/institutes/grid.252546.2", 
          "name": [
            "School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mobley", 
        "givenName": "Christopher Brooks", 
        "id": "sg:person.01154064651.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154064651.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849, USA", 
          "id": "http://www.grid.ac/institutes/grid.252546.2", 
          "name": [
            "School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fox", 
        "givenName": "Carlton D", 
        "id": "sg:person.01222200051.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01222200051.87"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849, USA", 
          "id": "http://www.grid.ac/institutes/grid.252546.2", 
          "name": [
            "School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ferguson", 
        "givenName": "Brian S", 
        "id": "sg:person.01150235765.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150235765.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Harrison School of Pharmacy, Auburn University, Auburn, AL, USA", 
          "id": "http://www.grid.ac/institutes/grid.252546.2", 
          "name": [
            "Harrison School of Pharmacy, Auburn University, Auburn, AL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Amin", 
        "givenName": "Rajesh H", 
        "id": "sg:person.01156430113.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156430113.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD, Australia", 
          "id": "http://www.grid.ac/institutes/grid.1023.0", 
          "name": [
            "School of Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dalbo", 
        "givenName": "Vincent J", 
        "id": "sg:person.0702073101.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702073101.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Metabolic Technologies, Inc, Ames, IA, USA", 
          "id": "http://www.grid.ac/institutes/grid.421284.9", 
          "name": [
            "Metabolic Technologies, Inc, Ames, IA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Baier", 
        "givenName": "Shawn", 
        "id": "sg:person.0623604304.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623604304.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Metabolic Technologies, Inc, Ames, IA, USA", 
          "id": "http://www.grid.ac/institutes/grid.421284.9", 
          "name": [
            "Metabolic Technologies, Inc, Ames, IA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rathmacher", 
        "givenName": "John A", 
        "id": "sg:person.01233306320.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233306320.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Health Sciences and Human Performance, University of Tampa, Tampa, FL, USA", 
          "id": "http://www.grid.ac/institutes/grid.267280.9", 
          "name": [
            "Department of Health Sciences and Human Performance, University of Tampa, Tampa, FL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wilson", 
        "givenName": "Jacob M", 
        "id": "sg:person.01164646604.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164646604.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849, USA", 
          "id": "http://www.grid.ac/institutes/grid.252546.2", 
          "name": [
            "School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Roberts", 
        "givenName": "Michael D", 
        "id": "sg:person.01366257433.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366257433.24"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/1550-2783-6-16", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035390763", 
          "https://doi.org/10.1186/1550-2783-6-16"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s004210050575", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005288674", 
          "https://doi.org/10.1007/s004210050575"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1743-7075-5-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008975244", 
          "https://doi.org/10.1186/1743-7075-5-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00394-012-0349-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041885736", 
          "https://doi.org/10.1007/s00394-012-0349-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1743-7075-8-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022571105", 
          "https://doi.org/10.1186/1743-7075-8-11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1022465203458", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035589145", 
          "https://doi.org/10.1023/a:1022465203458"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1016366409691", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040410166", 
          "https://doi.org/10.1023/a:1016366409691"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.2165/00007256-200030020-00004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022775338", 
          "https://doi.org/10.2165/00007256-200030020-00004"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-08-13", 
    "datePublishedReg": "2014-08-13", 
    "description": "BACKGROUND: The purpose of this study was to examine if L-leucine (Leu), \u03b2-hydroxy-\u03b2-methylbutyrate (HMB), or creatine monohydrate (Crea) prevented potential atrophic effects of myostatin (MSTN) on differentiated C2C12 myotubes.\nMETHODS: After four days of differentiation, myotubes were treated with MSTN (10\u00a0ng/ml) for two additional days and four treatment groups were studied: 1) 3x per day 10\u00a0mM Leu, 2) 3x per day 10\u00a0mM HMB, 3) 3x per day 10\u00a0mM Crea, 4) DM only. Myotubes treated with DM without MSTN were analyzed as the control condition (DM/CTL). Following treatment, cells were analyzed for total protein, DNA content, RNA content, muscle protein synthesis (MPS, SUnSET method), and fiber diameter. Separate batch treatments were analyzed for mRNA expression patterns of myostatin-related genes (Akirin-1/Mighty, Notch-1, Ski, MyoD) as well as atrogenes (MuRF-1, and MAFbx/Atrogin-1).\nRESULTS: MSTN decreased fiber diameter approximately 30% compared to DM/CTL myotubes (p\u2009<\u20090.001). Leu, HMB and Crea prevented MSTN-induced atrophy. MSTN did not decrease MPS levels compared to DM/CTL myotubes, but MSTN treatment decreased the mRNA expression of Akirin-1/Mighty by 27% (p\u2009<\u20090.001) and MyoD by 26% (p\u2009<\u20090.01) compared to DM/CTL myotubes. shRNA experiments confirmed that Mighty mRNA knockdown reduced myotube size, linking MSTN treatment to atrophy independent of MPS. Remarkably, MSTN\u2009+\u2009Leu and MSTN\u2009+\u2009HMB myotubes had similar Akirin-1/Mighty and MyoD mRNA levels compared to DM/CTL myotubes. Furthermore, MSTN\u2009+\u2009Crea myotubes exhibited a 36% (p\u2009<\u20090.05) and 86% (p\u2009<\u20090.001) increase in Akirin-1/Mighty mRNA compared to DM/CTL and MSTN-only treated myotubes, respectively.\nCONCLUSIONS: Leu, HMB and Crea may reduce MSTN-induced muscle fiber atrophy by influencing Akirin-1/Mighty mRNA expression patterns. Future studies are needed to examine if Leu, HMB and Crea independently or synergistically affect Akirin-1/Mighty expression, and how Akirin-1/Mighty expression mechanistically relates to skeletal muscle hypertrophy in vivo.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/1550-2783-11-38", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1034609", 
        "issn": [
          "1550-2783"
        ], 
        "name": "Journal of the International Society of Sports Nutrition", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "11"
      }
    ], 
    "keywords": [
      "day 10", 
      "creatine monohydrate", 
      "MSTN treatment", 
      "mRNA expression patterns", 
      "muscle protein synthesis", 
      "muscle fiber atrophy", 
      "skeletal muscle hypertrophy", 
      "atrophic effect", 
      "fiber atrophy", 
      "myotube atrophy", 
      "treatment groups", 
      "muscle hypertrophy", 
      "mRNA expression", 
      "atrophy", 
      "myotube size", 
      "expression patterns", 
      "C2C12 myotubes", 
      "additional days", 
      "mRNA levels", 
      "days of differentiation", 
      "MPS levels", 
      "treatment", 
      "total protein", 
      "myostatin", 
      "HMB", 
      "future studies", 
      "control condition", 
      "RNA content", 
      "myotubes", 
      "mRNA knockdown", 
      "DNA content", 
      "days", 
      "expression", 
      "protein synthesis", 
      "mRNA", 
      "hypertrophy", 
      "shRNA experiments", 
      "atrogenes", 
      "CTL", 
      "methylbutyrate", 
      "leucine", 
      "mM Leu", 
      "levels", 
      "MyoD mRNA levels", 
      "vivo", 
      "study", 
      "knockdown", 
      "Leu", 
      "cells", 
      "group", 
      "CreA", 
      "differentiation", 
      "patterns", 
      "DM", 
      "fiber diameter", 
      "protein", 
      "MyoD", 
      "genes", 
      "increase", 
      "diameter", 
      "effect", 
      "acid", 
      "MPS", 
      "purpose", 
      "hydroxy", 
      "content", 
      "monohydrate", 
      "conditions", 
      "size", 
      "synthesis", 
      "experiments", 
      "Mighty", 
      "batch treatment", 
      "potential atrophic effects", 
      "mM Crea", 
      "Separate batch treatments", 
      "myostatin-related genes", 
      "DM/CTL myotubes", 
      "CTL myotubes", 
      "Akirin-1/", 
      "Mighty mRNA knockdown", 
      "HMB myotubes", 
      "similar Akirin-1/Mighty", 
      "Akirin-1/Mighty", 
      "Crea myotubes", 
      "Akirin-1/Mighty mRNA", 
      "Mighty mRNA", 
      "DM/CTL", 
      "MSTN-induced muscle fiber atrophy", 
      "Akirin-1/Mighty mRNA expression patterns", 
      "Mighty mRNA expression patterns", 
      "Akirin-1/Mighty expression", 
      "Mighty expression", 
      "beta-methylbutyric acid"
    ], 
    "name": "L-leucine, beta-hydroxy-beta-methylbutyric acid (HMB) and creatine monohydrate prevent myostatin-induced Akirin-1/Mighty mRNA down-regulation and myotube atrophy", 
    "pagination": "38-38", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1001591525"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1550-2783-11-38"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25132809"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1550-2783-11-38", 
      "https://app.dimensions.ai/details/publication/pub.1001591525"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-11-01T18:23", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_642.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/1550-2783-11-38"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1550-2783-11-38'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1550-2783-11-38'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1550-2783-11-38'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1550-2783-11-38'


 

This table displays all metadata directly associated to this object as RDF triples.

254 TRIPLES      22 PREDICATES      128 URIs      112 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1550-2783-11-38 schema:about anzsrc-for:11
2 anzsrc-for:1103
3 schema:author Nadd5a7e386e04bca9d8f56c943f8ee8a
4 schema:citation sg:pub.10.1007/s00394-012-0349-x
5 sg:pub.10.1007/s004210050575
6 sg:pub.10.1023/a:1016366409691
7 sg:pub.10.1023/a:1022465203458
8 sg:pub.10.1186/1550-2783-6-16
9 sg:pub.10.1186/1743-7075-5-1
10 sg:pub.10.1186/1743-7075-8-11
11 sg:pub.10.2165/00007256-200030020-00004
12 schema:datePublished 2014-08-13
13 schema:datePublishedReg 2014-08-13
14 schema:description BACKGROUND: The purpose of this study was to examine if L-leucine (Leu), β-hydroxy-β-methylbutyrate (HMB), or creatine monohydrate (Crea) prevented potential atrophic effects of myostatin (MSTN) on differentiated C2C12 myotubes. METHODS: After four days of differentiation, myotubes were treated with MSTN (10 ng/ml) for two additional days and four treatment groups were studied: 1) 3x per day 10 mM Leu, 2) 3x per day 10 mM HMB, 3) 3x per day 10 mM Crea, 4) DM only. Myotubes treated with DM without MSTN were analyzed as the control condition (DM/CTL). Following treatment, cells were analyzed for total protein, DNA content, RNA content, muscle protein synthesis (MPS, SUnSET method), and fiber diameter. Separate batch treatments were analyzed for mRNA expression patterns of myostatin-related genes (Akirin-1/Mighty, Notch-1, Ski, MyoD) as well as atrogenes (MuRF-1, and MAFbx/Atrogin-1). RESULTS: MSTN decreased fiber diameter approximately 30% compared to DM/CTL myotubes (p < 0.001). Leu, HMB and Crea prevented MSTN-induced atrophy. MSTN did not decrease MPS levels compared to DM/CTL myotubes, but MSTN treatment decreased the mRNA expression of Akirin-1/Mighty by 27% (p < 0.001) and MyoD by 26% (p < 0.01) compared to DM/CTL myotubes. shRNA experiments confirmed that Mighty mRNA knockdown reduced myotube size, linking MSTN treatment to atrophy independent of MPS. Remarkably, MSTN + Leu and MSTN + HMB myotubes had similar Akirin-1/Mighty and MyoD mRNA levels compared to DM/CTL myotubes. Furthermore, MSTN + Crea myotubes exhibited a 36% (p < 0.05) and 86% (p < 0.001) increase in Akirin-1/Mighty mRNA compared to DM/CTL and MSTN-only treated myotubes, respectively. CONCLUSIONS: Leu, HMB and Crea may reduce MSTN-induced muscle fiber atrophy by influencing Akirin-1/Mighty mRNA expression patterns. Future studies are needed to examine if Leu, HMB and Crea independently or synergistically affect Akirin-1/Mighty expression, and how Akirin-1/Mighty expression mechanistically relates to skeletal muscle hypertrophy in vivo.
15 schema:genre article
16 schema:inLanguage en
17 schema:isAccessibleForFree true
18 schema:isPartOf N6a2cad235ddd43428d610f9ca0913913
19 N8626af7710e24234b29be045a3b34659
20 sg:journal.1034609
21 schema:keywords Akirin-1/
22 Akirin-1/Mighty
23 Akirin-1/Mighty expression
24 Akirin-1/Mighty mRNA
25 Akirin-1/Mighty mRNA expression patterns
26 C2C12 myotubes
27 CTL
28 CTL myotubes
29 CreA
30 Crea myotubes
31 DM
32 DM/CTL
33 DM/CTL myotubes
34 DNA content
35 HMB
36 HMB myotubes
37 Leu
38 MPS
39 MPS levels
40 MSTN treatment
41 MSTN-induced muscle fiber atrophy
42 Mighty
43 Mighty expression
44 Mighty mRNA
45 Mighty mRNA expression patterns
46 Mighty mRNA knockdown
47 MyoD
48 MyoD mRNA levels
49 RNA content
50 Separate batch treatments
51 acid
52 additional days
53 atrogenes
54 atrophic effect
55 atrophy
56 batch treatment
57 beta-methylbutyric acid
58 cells
59 conditions
60 content
61 control condition
62 creatine monohydrate
63 day 10
64 days
65 days of differentiation
66 diameter
67 differentiation
68 effect
69 experiments
70 expression
71 expression patterns
72 fiber atrophy
73 fiber diameter
74 future studies
75 genes
76 group
77 hydroxy
78 hypertrophy
79 increase
80 knockdown
81 leucine
82 levels
83 mM Crea
84 mM Leu
85 mRNA
86 mRNA expression
87 mRNA expression patterns
88 mRNA knockdown
89 mRNA levels
90 methylbutyrate
91 monohydrate
92 muscle fiber atrophy
93 muscle hypertrophy
94 muscle protein synthesis
95 myostatin
96 myostatin-related genes
97 myotube atrophy
98 myotube size
99 myotubes
100 patterns
101 potential atrophic effects
102 protein
103 protein synthesis
104 purpose
105 shRNA experiments
106 similar Akirin-1/Mighty
107 size
108 skeletal muscle hypertrophy
109 study
110 synthesis
111 total protein
112 treatment
113 treatment groups
114 vivo
115 schema:name L-leucine, beta-hydroxy-beta-methylbutyric acid (HMB) and creatine monohydrate prevent myostatin-induced Akirin-1/Mighty mRNA down-regulation and myotube atrophy
116 schema:pagination 38-38
117 schema:productId N3773c78da29947b3b04fa1218b4a2ce5
118 N4bf544dd19ac4bc9978bd19f378c7699
119 Nece9dc1c678c4a3f82937452e59af2c8
120 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001591525
121 https://doi.org/10.1186/1550-2783-11-38
122 schema:sdDatePublished 2021-11-01T18:23
123 schema:sdLicense https://scigraph.springernature.com/explorer/license/
124 schema:sdPublisher N100a04b9e9a94e3392ddee1bdd860251
125 schema:url https://doi.org/10.1186/1550-2783-11-38
126 sgo:license sg:explorer/license/
127 sgo:sdDataset articles
128 rdf:type schema:ScholarlyArticle
129 N100a04b9e9a94e3392ddee1bdd860251 schema:name Springer Nature - SN SciGraph project
130 rdf:type schema:Organization
131 N336ce94fef3c45bba1777b7dc64f8e7a rdf:first sg:person.01222200051.87
132 rdf:rest Na8de2843549c4b3980bb9f3eaed22126
133 N33a44317c85b48c3b80a98035027e7d3 rdf:first sg:person.01233306320.98
134 rdf:rest Ndf7c582ca4624e93b45c2334d2c8e0c2
135 N3773c78da29947b3b04fa1218b4a2ce5 schema:name dimensions_id
136 schema:value pub.1001591525
137 rdf:type schema:PropertyValue
138 N4bf544dd19ac4bc9978bd19f378c7699 schema:name doi
139 schema:value 10.1186/1550-2783-11-38
140 rdf:type schema:PropertyValue
141 N6a2cad235ddd43428d610f9ca0913913 schema:issueNumber 1
142 rdf:type schema:PublicationIssue
143 N74cd5cb11a0247c6a4e6b2d501d68fc0 rdf:first sg:person.01156430113.42
144 rdf:rest N9d5ecbc7b8d248ddabf6474a8e351830
145 N8626af7710e24234b29be045a3b34659 schema:volumeNumber 11
146 rdf:type schema:PublicationVolume
147 N8d7f184466fc4c689ef6dce7ce42768f rdf:first sg:person.01366257433.24
148 rdf:rest rdf:nil
149 N9d5ecbc7b8d248ddabf6474a8e351830 rdf:first sg:person.0702073101.48
150 rdf:rest Na6f0f8aaf95b4b818a9e158d5061ad42
151 Na6f0f8aaf95b4b818a9e158d5061ad42 rdf:first sg:person.0623604304.79
152 rdf:rest N33a44317c85b48c3b80a98035027e7d3
153 Na8de2843549c4b3980bb9f3eaed22126 rdf:first sg:person.01150235765.47
154 rdf:rest N74cd5cb11a0247c6a4e6b2d501d68fc0
155 Nadd5a7e386e04bca9d8f56c943f8ee8a rdf:first sg:person.01154064651.37
156 rdf:rest N336ce94fef3c45bba1777b7dc64f8e7a
157 Ndf7c582ca4624e93b45c2334d2c8e0c2 rdf:first sg:person.01164646604.47
158 rdf:rest N8d7f184466fc4c689ef6dce7ce42768f
159 Nece9dc1c678c4a3f82937452e59af2c8 schema:name pubmed_id
160 schema:value 25132809
161 rdf:type schema:PropertyValue
162 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
163 schema:name Medical and Health Sciences
164 rdf:type schema:DefinedTerm
165 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
166 schema:name Clinical Sciences
167 rdf:type schema:DefinedTerm
168 sg:journal.1034609 schema:issn 1550-2783
169 schema:name Journal of the International Society of Sports Nutrition
170 schema:publisher Springer Nature
171 rdf:type schema:Periodical
172 sg:person.01150235765.47 schema:affiliation grid-institutes:grid.252546.2
173 schema:familyName Ferguson
174 schema:givenName Brian S
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150235765.47
176 rdf:type schema:Person
177 sg:person.01154064651.37 schema:affiliation grid-institutes:grid.252546.2
178 schema:familyName Mobley
179 schema:givenName Christopher Brooks
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154064651.37
181 rdf:type schema:Person
182 sg:person.01156430113.42 schema:affiliation grid-institutes:grid.252546.2
183 schema:familyName Amin
184 schema:givenName Rajesh H
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156430113.42
186 rdf:type schema:Person
187 sg:person.01164646604.47 schema:affiliation grid-institutes:grid.267280.9
188 schema:familyName Wilson
189 schema:givenName Jacob M
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164646604.47
191 rdf:type schema:Person
192 sg:person.01222200051.87 schema:affiliation grid-institutes:grid.252546.2
193 schema:familyName Fox
194 schema:givenName Carlton D
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01222200051.87
196 rdf:type schema:Person
197 sg:person.01233306320.98 schema:affiliation grid-institutes:grid.421284.9
198 schema:familyName Rathmacher
199 schema:givenName John A
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233306320.98
201 rdf:type schema:Person
202 sg:person.01366257433.24 schema:affiliation grid-institutes:grid.252546.2
203 schema:familyName Roberts
204 schema:givenName Michael D
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366257433.24
206 rdf:type schema:Person
207 sg:person.0623604304.79 schema:affiliation grid-institutes:grid.421284.9
208 schema:familyName Baier
209 schema:givenName Shawn
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623604304.79
211 rdf:type schema:Person
212 sg:person.0702073101.48 schema:affiliation grid-institutes:grid.1023.0
213 schema:familyName Dalbo
214 schema:givenName Vincent J
215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702073101.48
216 rdf:type schema:Person
217 sg:pub.10.1007/s00394-012-0349-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041885736
218 https://doi.org/10.1007/s00394-012-0349-x
219 rdf:type schema:CreativeWork
220 sg:pub.10.1007/s004210050575 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005288674
221 https://doi.org/10.1007/s004210050575
222 rdf:type schema:CreativeWork
223 sg:pub.10.1023/a:1016366409691 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040410166
224 https://doi.org/10.1023/a:1016366409691
225 rdf:type schema:CreativeWork
226 sg:pub.10.1023/a:1022465203458 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035589145
227 https://doi.org/10.1023/a:1022465203458
228 rdf:type schema:CreativeWork
229 sg:pub.10.1186/1550-2783-6-16 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035390763
230 https://doi.org/10.1186/1550-2783-6-16
231 rdf:type schema:CreativeWork
232 sg:pub.10.1186/1743-7075-5-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008975244
233 https://doi.org/10.1186/1743-7075-5-1
234 rdf:type schema:CreativeWork
235 sg:pub.10.1186/1743-7075-8-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022571105
236 https://doi.org/10.1186/1743-7075-8-11
237 rdf:type schema:CreativeWork
238 sg:pub.10.2165/00007256-200030020-00004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022775338
239 https://doi.org/10.2165/00007256-200030020-00004
240 rdf:type schema:CreativeWork
241 grid-institutes:grid.1023.0 schema:alternateName School of Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD, Australia
242 schema:name School of Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD, Australia
243 rdf:type schema:Organization
244 grid-institutes:grid.252546.2 schema:alternateName Harrison School of Pharmacy, Auburn University, Auburn, AL, USA
245 School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849, USA
246 schema:name Harrison School of Pharmacy, Auburn University, Auburn, AL, USA
247 School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849, USA
248 rdf:type schema:Organization
249 grid-institutes:grid.267280.9 schema:alternateName Department of Health Sciences and Human Performance, University of Tampa, Tampa, FL, USA
250 schema:name Department of Health Sciences and Human Performance, University of Tampa, Tampa, FL, USA
251 rdf:type schema:Organization
252 grid-institutes:grid.421284.9 schema:alternateName Metabolic Technologies, Inc, Ames, IA, USA
253 schema:name Metabolic Technologies, Inc, Ames, IA, USA
254 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...