Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banks View Full Text


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Article Info

DATE

2012-05-16

AUTHORS

Bianca Polchow, Kati Kebbel, Gerno Schmiedeknecht, Anne Reichardt, Wolfgang Henrich, Roland Hetzer, Cora Lueders

ABSTRACT

BackgroundIn vitro fabricated tissue engineered vascular constructs could provide an alternative to conventional substitutes. A crucial factor for tissue engineering of vascular constructs is an appropriate cell source. Vascular cells from the human umbilical cord can be directly isolated and cryopreserved until needed. Currently no cell bank for human vascular cells is available. Therefore, the establishment of a future human vascular cell bank conforming to good manufacturing practice (GMP) conditions is desirable for therapeutic applications such as tissue engineered cardiovascular constructs.Materials and methodsA fundamental step was the adaption of conventional research and development starting materials to GMP compliant starting materials. Human umbilical cord artery derived cells (HUCAC) and human umbilical vein endothelial cells (HUVEC) were isolated, cultivated, cryopreserved (short- and long-term) directly after primary culture and recultivated subsequently. Cell viability, expression of cellular markers and proliferation potential of fresh and cryopreserved cells were studied using trypan blue staining, flow cytometry analysis, immunofluorescence staining and proliferation assays. Statistical analyses were performed using Student’s t-test.ResultsSufficient numbers of isolated cells with acceptable viabilities and homogenous expression of cellular markers confirmed that the isolation procedure was successful using GMP compliant starting materials. The influence of cryopreservation was marginal, because cryopreserved cells mostly maintain phenotypic and functional characteristics similar to those of fresh cells. Phenotypic studies revealed that fresh cultivated and cryopreserved HUCAC were positive for alpha smooth muscle actin, CD90, CD105, CD73, CD29, CD44, CD166 and negative for smoothelin. HUVEC expressed CD31, CD146, CD105 and CD144 but not alpha smooth muscle actin. Functional analysis demonstrated acceptable viability and sufficient proliferation properties of cryopreserved HUCAC and HUVEC.ConclusionAdaptation of cell isolation, cultivation and cryopreservation to GMP compliant starting materials was successful. Cryopreservation did not influence cell properties with lasting impact, confirming that the application of vascular cells from the human umbilical cord is feasible for cell banking. A specific cellular marker expression profile was established for HUCAC and HUVEC using flow cytometry analysis, applicable as a GMP compliant quality control. Use of these cells for the future fabrication of advanced therapy medicinal products GMP conditions are required by the regulatory authority. More... »

PAGES

98

References to SciGraph publications

  • 2009-04-24. Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels in JOURNAL OF TRANSLATIONAL MEDICINE
  • 2006-10-12. Heart Valve Tissue Engineering: Concepts, Approaches, Progress, and Challenges in ANNALS OF BIOMEDICAL ENGINEERING
  • 2003-10. Anwendung von frisch isolierten und kryokonservierten Nabelschnurzellen für das Tissue Engineering von kardiovaskulären Geweben in ZEITSCHRIFT FÜR HERZ-,THORAX- UND GEFÄßCHIRURGIE
  • 2006-06. Umbilical cord cells as a source of cardiovascular tissue engineering in STEM CELL REVIEWS AND REPORTS
  • 2009-10-05. Cord blood banking for clinical transplantation in BONE MARROW TRANSPLANTATION
  • 2002. SIMULTANEOUS ISOLATION OF ENDOTHELIAL AND SMOOTH MUSCLE CELLS FROM HUMAN UMBILICAL ARTERY OR VEIN AND THEIR GROWTH RESPONSE TO LOW-DENSITY LIPOPROTEINS in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2010-08-06. Intestinal Mesenchymal Cells in CURRENT GASTROENTEROLOGY REPORTS
  • 2011-06-22. The advanced therapy classification procedure in BUNDESGESUNDHEITSBLATT - GESUNDHEITSFORSCHUNG - GESUNDHEITSSCHUTZ
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1186/1479-5876-10-98

    DOI

    http://dx.doi.org/10.1186/1479-5876-10-98

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015477055

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22591741


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    27 schema:description BackgroundIn vitro fabricated tissue engineered vascular constructs could provide an alternative to conventional substitutes. A crucial factor for tissue engineering of vascular constructs is an appropriate cell source. Vascular cells from the human umbilical cord can be directly isolated and cryopreserved until needed. Currently no cell bank for human vascular cells is available. Therefore, the establishment of a future human vascular cell bank conforming to good manufacturing practice (GMP) conditions is desirable for therapeutic applications such as tissue engineered cardiovascular constructs.Materials and methodsA fundamental step was the adaption of conventional research and development starting materials to GMP compliant starting materials. Human umbilical cord artery derived cells (HUCAC) and human umbilical vein endothelial cells (HUVEC) were isolated, cultivated, cryopreserved (short- and long-term) directly after primary culture and recultivated subsequently. Cell viability, expression of cellular markers and proliferation potential of fresh and cryopreserved cells were studied using trypan blue staining, flow cytometry analysis, immunofluorescence staining and proliferation assays. Statistical analyses were performed using Student’s t-test.ResultsSufficient numbers of isolated cells with acceptable viabilities and homogenous expression of cellular markers confirmed that the isolation procedure was successful using GMP compliant starting materials. The influence of cryopreservation was marginal, because cryopreserved cells mostly maintain phenotypic and functional characteristics similar to those of fresh cells. Phenotypic studies revealed that fresh cultivated and cryopreserved HUCAC were positive for alpha smooth muscle actin, CD90, CD105, CD73, CD29, CD44, CD166 and negative for smoothelin. HUVEC expressed CD31, CD146, CD105 and CD144 but not alpha smooth muscle actin. Functional analysis demonstrated acceptable viability and sufficient proliferation properties of cryopreserved HUCAC and HUVEC.ConclusionAdaptation of cell isolation, cultivation and cryopreservation to GMP compliant starting materials was successful. Cryopreservation did not influence cell properties with lasting impact, confirming that the application of vascular cells from the human umbilical cord is feasible for cell banking. A specific cellular marker expression profile was established for HUCAC and HUVEC using flow cytometry analysis, applicable as a GMP compliant quality control. Use of these cells for the future fabrication of advanced therapy medicinal products GMP conditions are required by the regulatory authority.
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    91 factors
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    93 fresh cells
    94 functional analysis
    95 functional characteristics
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