JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Proliferation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colony-Forming Units Assay", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cryopreservation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Extracellular Matrix Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fetal Blood", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Guideline Adherence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Human Umbilical Vein Endothelial Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mesenchymal Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tissue Banks", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Umbilical Arteries", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "German Heart Institute Berlin, Department of Cardiothoracic and Vascular Surgery, Laboratory for Tissue Engineering, Augustenburger Platz 1, 13353, Berlin, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418209.6", 
          "name": [
            "German Heart Institute Berlin, Department of Cardiothoracic and Vascular Surgery, Laboratory for Tissue Engineering, Augustenburger Platz 1, 13353, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Polchow", 
        "givenName": "Bianca", 
        "id": "sg:person.0672345603.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672345603.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Fraunhofer Institute for Cell Therapy and Immunology IZI, Department of Cell Engineering/GMP, Perlickstr. 1, 04103, Leipzig, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418008.5", 
          "name": [
            "Fraunhofer Institute for Cell Therapy and Immunology IZI, Department of Cell Engineering/GMP, Perlickstr. 1, 04103, Leipzig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kebbel", 
        "givenName": "Kati", 
        "id": "sg:person.0740461003.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740461003.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Fraunhofer Institute for Cell Therapy and Immunology IZI, Department of Cell Engineering/GMP, Perlickstr. 1, 04103, Leipzig, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418008.5", 
          "name": [
            "Fraunhofer Institute for Cell Therapy and Immunology IZI, Department of Cell Engineering/GMP, Perlickstr. 1, 04103, Leipzig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schmiedeknecht", 
        "givenName": "Gerno", 
        "id": "sg:person.014062165040.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014062165040.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "German Heart Institute Berlin, Department of Cardiothoracic and Vascular Surgery, Laboratory for Tissue Engineering, Augustenburger Platz 1, 13353, Berlin, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418209.6", 
          "name": [
            "German Heart Institute Berlin, Department of Cardiothoracic and Vascular Surgery, Laboratory for Tissue Engineering, Augustenburger Platz 1, 13353, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Reichardt", 
        "givenName": "Anne", 
        "id": "sg:person.01237251203.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237251203.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charit\u00e9-Universit\u00e4tsmedizin Berlin, Department of Obstetrics, Augustenburger Platz 1, 13353, Berlin, Germany", 
          "id": "http://www.grid.ac/institutes/grid.6363.0", 
          "name": [
            "Charit\u00e9-Universit\u00e4tsmedizin Berlin, Department of Obstetrics, Augustenburger Platz 1, 13353, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Henrich", 
        "givenName": "Wolfgang", 
        "id": "sg:person.0747560146.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0747560146.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "German Heart Institute Berlin, Department of Cardiothoracic and Vascular Surgery, Laboratory for Tissue Engineering, Augustenburger Platz 1, 13353, Berlin, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418209.6", 
          "name": [
            "German Heart Institute Berlin, Department of Cardiothoracic and Vascular Surgery, Laboratory for Tissue Engineering, Augustenburger Platz 1, 13353, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hetzer", 
        "givenName": "Roland", 
        "id": "sg:person.01365311760.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01365311760.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory for Tissue Engineering German Heart Institute Berlin, Augustenburger Platz 1, D-13353, Berlin, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418209.6", 
          "name": [
            "German Heart Institute Berlin, Department of Cardiothoracic and Vascular Surgery, Laboratory for Tissue Engineering, Augustenburger Platz 1, 13353, Berlin, Germany", 
            "Laboratory for Tissue Engineering German Heart Institute Berlin, Augustenburger Platz 1, D-13353, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lueders", 
        "givenName": "Cora", 
        "id": "sg:person.01227606277.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227606277.17"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s12015-006-0014-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040745936", 
          "https://doi.org/10.1007/s12015-006-0014-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11894-010-0135-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005397169", 
          "https://doi.org/10.1007/s11894-010-0135-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10439-006-9163-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051925160", 
          "https://doi.org/10.1007/s10439-006-9163-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00103-011-1309-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025386064", 
          "https://doi.org/10.1007/s00103-011-1309-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1479-5876-7-30", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046545595", 
          "https://doi.org/10.1186/1479-5876-7-30"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/bmt.2009.281", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030491311", 
          "https://doi.org/10.1038/bmt.2009.281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1290/1071-2690(2002)038<0265:sioeas>2.0.co;2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020503182", 
          "https://doi.org/10.1290/1071-2690(2002)038<0265:sioeas>2.0.co;2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00398-003-0410-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045071732", 
          "https://doi.org/10.1007/s00398-003-0410-4"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-05-16", 
    "datePublishedReg": "2012-05-16", 
    "description": "BackgroundIn vitro fabricated tissue engineered vascular constructs could provide an alternative to conventional substitutes. A crucial factor for tissue engineering of vascular constructs is an appropriate cell source. Vascular cells from the human umbilical cord can be directly isolated and cryopreserved until needed. Currently no cell bank for human vascular cells is available. Therefore, the establishment of a future human vascular cell bank conforming to good manufacturing practice (GMP) conditions is desirable for therapeutic applications such as tissue engineered cardiovascular constructs.Materials and methodsA fundamental step was the adaption of conventional research and development starting materials to GMP compliant starting materials. Human umbilical cord artery derived cells (HUCAC) and human umbilical vein endothelial cells (HUVEC) were isolated, cultivated, cryopreserved (short- and long-term) directly after primary culture and recultivated subsequently. Cell viability, expression of cellular markers and proliferation potential of fresh and cryopreserved cells were studied using trypan blue staining, flow cytometry analysis, immunofluorescence staining and proliferation assays. Statistical analyses were performed using Student\u2019s\u2009t-test.ResultsSufficient numbers of isolated cells with acceptable viabilities and homogenous expression of cellular markers confirmed that the isolation procedure was successful using GMP compliant starting materials. The influence of cryopreservation was marginal, because cryopreserved cells mostly maintain phenotypic and functional characteristics similar to those of fresh cells. Phenotypic studies revealed that fresh cultivated and cryopreserved HUCAC were positive for alpha smooth muscle actin, CD90, CD105, CD73, CD29, CD44, CD166 and negative for smoothelin. HUVEC expressed CD31, CD146, CD105 and CD144 but not alpha smooth muscle actin. Functional analysis demonstrated acceptable viability and sufficient proliferation properties of cryopreserved HUCAC and HUVEC.ConclusionAdaptation of cell isolation, cultivation and cryopreservation to GMP compliant starting materials was successful. Cryopreservation did not influence cell properties with lasting impact, confirming that the application of vascular cells from the human umbilical cord is feasible for cell banking. A specific cellular marker expression profile was established for HUCAC and HUVEC using flow cytometry analysis, applicable as a GMP compliant quality control. Use of these cells for the future fabrication of advanced therapy medicinal products GMP conditions are required by the regulatory authority.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/1479-5876-10-98", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1032886", 
        "issn": [
          "1479-5876"
        ], 
        "name": "Journal of Translational Medicine", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "keywords": [
      "human umbilical vein endothelial cells", 
      "vascular cells", 
      "flow cytometry analysis", 
      "alpha-smooth muscle actin", 
      "human umbilical cord", 
      "cytometry analysis", 
      "cellular markers", 
      "cell banks", 
      "human vascular cells", 
      "umbilical vein endothelial cells", 
      "functional analysis", 
      "expression profiles", 
      "appropriate cell source", 
      "smooth muscle actin", 
      "umbilical cord cells", 
      "vein endothelial cells", 
      "phenotypic studies", 
      "muscle actin", 
      "marker expression profiles", 
      "vascular constructs", 
      "human umbilical cord arteries", 
      "trypan blue staining", 
      "homogenous expression", 
      "proliferation potential", 
      "cell properties", 
      "cell viability", 
      "actin", 
      "proliferation assays", 
      "cell source", 
      "cells", 
      "endothelial cells", 
      "functional characteristics", 
      "primary cultures", 
      "influence of cryopreservation", 
      "GMP", 
      "conventional substitutes", 
      "cell isolation", 
      "proliferation properties", 
      "cardiovascular constructs", 
      "therapeutic applications", 
      "isolation procedure", 
      "expression", 
      "acceptable viability", 
      "blue staining", 
      "viability", 
      "CD105", 
      "cryopreserved cells", 
      "tissue engineering", 
      "cord cells", 
      "markers", 
      "tissue", 
      "umbilical cord artery", 
      "cryopreservation", 
      "umbilical cord", 
      "constructs", 
      "CD166", 
      "fresh cells", 
      "smoothelin", 
      "fundamental step", 
      "CD90", 
      "cell banking", 
      "staining", 
      "CD146", 
      "CD29", 
      "quality control", 
      "assays", 
      "CD44", 
      "cultivation", 
      "CD144", 
      "CD73", 
      "isolation", 
      "establishment", 
      "GMP conditions", 
      "analysis", 
      "crucial factor", 
      "adaption", 
      "culture", 
      "conditions", 
      "cord artery", 
      "CD31", 
      "banks", 
      "development", 
      "factors", 
      "potential", 
      "profile", 
      "engineering", 
      "good manufacturing practice conditions", 
      "step", 
      "number", 
      "control", 
      "source", 
      "cord", 
      "study", 
      "impact", 
      "statistical analysis", 
      "properties", 
      "applications", 
      "influence", 
      "characteristics", 
      "use", 
      "research", 
      "future fabrication", 
      "substitute", 
      "alternative", 
      "materials", 
      "regulatory authorities", 
      "BackgroundIn", 
      "conventional research", 
      "fabrication", 
      "practice conditions", 
      "procedure", 
      "t-test", 
      "artery", 
      "banking", 
      "authorities", 
      "students"
    ], 
    "name": "Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banks", 
    "pagination": "98", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1015477055"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1479-5876-10-98"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22591741"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1479-5876-10-98", 
      "https://app.dimensions.ai/details/publication/pub.1015477055"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-10T10:06", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220509/entities/gbq_results/article/article_561.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/1479-5876-10-98"
  }
]

Download the RDF metadata as:  json-ld nt turtle xml License info

This table displays all metadata directly associated to this object as RDF triples.

311 TRIPLES      22 PREDICATES      163 URIs      148 LITERALS      21 BLANK NODES