ARTD10 substrate identification on protein microarrays: regulation of GSK3β by mono-ADP-ribosylation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-12

AUTHORS

Karla LH Feijs, Henning Kleine, Anne Braczynski, Alexandra H Forst, Nicolas Herzog, Patricia Verheugd, Ulrike Linzen, Elisabeth Kremmer, Bernhard Lüscher

ABSTRACT

BACKGROUND: Although ADP-ribosylation has been described five decades ago, only recently a distinction has been made between eukaryotic intracellular poly- and mono-ADP-ribosylating enzymes. Poly-ADP-ribosylation by ARTD1 (formerly PARP1) is best known for its role in DNA damage repair. Other polymer forming enzymes are ARTD2 (formerly PARP2), ARTD3 (formerly PARP3) and ARTD5/6 (formerly Tankyrase 1/2), the latter being involved in Wnt signaling and regulation of 3BP2. Thus several different functions of poly-ADP-ribosylation have been well described whereas intracellular mono-ADP-ribosylation is currently largely undefined. It is for example not known which proteins function as substrate for the different mono-ARTDs. This is partially due to lack of suitable reagents to study mono-ADP-ribosylation, which limits the current understanding of this post-translational modification. RESULTS: We have optimized a novel screening method employing protein microarrays, ProtoArrays®, applied here for the identification of substrates of ARTD10 (formerly PARP10) and ARTD8 (formerly PARP14). The results of this substrate screen were validated using in vitro ADP-ribosylation assays with recombinant proteins. Further analysis of the novel ARTD10 substrate GSK3β revealed mono-ADP-ribosylation as a regulatory mechanism of kinase activity by non-competitive inhibition in vitro. Additionally, manipulation of the ARTD10 levels in cells accordingly influenced GSK3β activity. Together these data provide the first evidence for a role of endogenous mono-ADP-ribosylation in intracellular signaling. CONCLUSIONS: Our findings indicate that substrates of ADP-ribosyltransferases can be identified using protein microarrays. The discovered substrates of ARTD10 and ARTD8 provide the first sets of proteins that are modified by mono-ADP-ribosyltransferases in vitro. By studying one of the ARTD10 substrates more closely, the kinase GSK3β, we identified mono-ADP-ribosylation as a negative regulator of kinase activity. More... »

PAGES

5

References to SciGraph publications

  • 2011-09. The structure and catalytic mechanism of a poly(ADP-ribose) glycohydrolase in NATURE
  • 2001-10. The renaissance of GSK3 in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2011. Identification of Distinct Amino Acids as ADP-Ribose Acceptor Sites by Mass Spectrometry in POLY(ADP-RIBOSE) POLYMERASE
  • 2012-12. Dynamic subcellular localization of the mono-ADP-ribosyltransferase ARTD10 and interaction with the ubiquitin receptor p62 in CELL COMMUNICATION AND SIGNALING
  • 2010-08. GSK3 signalling in neural development in NATURE REVIEWS NEUROSCIENCE
  • 2006-07. Poly(ADP-ribose): novel functions for an old molecule in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2008-01. Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins in NATURE
  • 2008-12. Protein kinase substrate identification on functional protein arrays in BMC BIOTECHNOLOGY
  • 1995-04. Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos in NATURE
  • 2012-07. New insights into the molecular and cellular functions of poly(ADP-ribose) and PARPs in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2009-10. Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling in NATURE
  • 2009-09. A macrodomain-containing histone rearranges chromatin upon sensing PARP1 activation in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2005-03. PARP-10, a novel Myc-interacting protein with poly(ADP-ribose) polymerase activity, inhibits transformation in ONCOGENE
  • 2012-01. Structure and mechanism of a canonical poly(ADP-ribose) glycohydrolase in NATURE COMMUNICATIONS
  • 2011-05. RNF146 is a poly(ADP-ribose)-directed E3 ligase that regulates axin degradation and Wnt signalling in NATURE CELL BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1478-811x-11-5

    DOI

    http://dx.doi.org/10.1186/1478-811x-11-5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1018884977

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23332125


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