α-Linolenic acid prevents endoplasmic reticulum stress-mediated apoptosis of stearic acid lipotoxicity on primary rat hepatocytes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-12

AUTHORS

Yong Zhang, Lei Dong, Xia Yang, Hongyang Shi, Li Zhang

ABSTRACT

AIMS: Lipid accumulation in non-adipose tissues leads to cell dysfunction and apoptosis, a phenomenon known as lipotoxicity. Unsaturated fatty acids may offset the lipotoxicity associated with saturated fatty acids. Stearic acid induced endoplasmic reticulum (ER) stress and caused apoptotic and necrotic cell death in the primary rat hepatocytes. METHODS: Cell viability was investigated using MTT assay, and apoptosis was evaluated with Hoechst 33342 staining. Western blot analysis was used to examine the changes in the expression levels of glucose regulated protein 78 (GRP78), glucose regulated protein 94 (GRP94), and C/EBP homologous protein (CHOP). Caspase-3 activity was evaluated using a Caspase-3 substrate kit. RESULTS: We have studied the ability of α-linolenic acid to prevent endoplasmic reticulum stress-mediated apoptosis of rat hepatocytes elicited by stearic acid and thapsigargin. Incubation of primary rat hepatocytes for 16 h with stearic acid produced a significant increase in cell death. Stearic acid also increased levels of three indicators of ER stress -- GRP78, CHOP, and GRP94. α-Linolenic acid distinctly reduced cell death and levels of all three indicators of ER stress brought about by stearic acid. Thapsigargin, which induces ER stress produced similar effects to those obtained using stearic acid; its effects were partly reversed by α-linolenic acid. CONCLUSION: These results suggest that α-linolenic acid prevents ER stress-mediated apoptosis of stearic acid lipotoxicity on primary rat hepatocytes might become a target to develop new antiapoptotic compounds in nonalcoholic fatty liver disease (NAFLD). More... »

PAGES

81

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1476-511x-10-81

DOI

http://dx.doi.org/10.1186/1476-511x-10-81

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002364320

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21592363


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endoplasmic Reticulum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepatocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protective Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats, Sprague-Dawley", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stearic Acids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stress, Physiological", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thapsigargin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "alpha-Linolenic Acid", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Xi'an Jiaotong University", 
          "id": "https://www.grid.ac/institutes/grid.452672.0", 
          "name": [
            "Department of Gastroenterology, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Yong", 
        "id": "sg:person.01131633214.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131633214.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Xi'an Jiaotong University", 
          "id": "https://www.grid.ac/institutes/grid.452672.0", 
          "name": [
            "Department of Gastroenterology, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dong", 
        "givenName": "Lei", 
        "id": "sg:person.01122713516.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122713516.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Xi'an Jiaotong University", 
          "id": "https://www.grid.ac/institutes/grid.452672.0", 
          "name": [
            "Department of Respiration, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Xia", 
        "id": "sg:person.01246061614.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01246061614.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Xi'an Jiaotong University", 
          "id": "https://www.grid.ac/institutes/grid.452672.0", 
          "name": [
            "Department of Respiration, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shi", 
        "givenName": "Hongyang", 
        "id": "sg:person.01317420371.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317420371.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Xi'an Jiaotong University", 
          "id": "https://www.grid.ac/institutes/grid.452672.0", 
          "name": [
            "Department of Gastroenterology, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Li", 
        "id": "sg:person.01234403427.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234403427.16"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1006/jsre.2000.5959", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000528624"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cbf.1667", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004420059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cbf.1667", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004420059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0027-5107(03)00134-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007123807"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0027-5107(03)00134-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007123807"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1388-1981(02)00342-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008295160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1388-1981(02)00342-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008295160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpendo.00478.2007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009556678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m510660200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009663644"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0630588100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017297859"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0091-679x(08)61797-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018711343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci23621", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021475977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00125-005-1861-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022928175", 
          "https://doi.org/10.1007/s00125-005-1861-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00125-005-1861-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022928175", 
          "https://doi.org/10.1007/s00125-005-1861-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00125-005-1861-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022928175", 
          "https://doi.org/10.1007/s00125-005-1861-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpendo.00644.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024855324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpheart.00980.2009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025091789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1103160", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025886111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003246-198907000-00020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026048686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003246-198907000-00020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026048686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3700603", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026270369", 
          "https://doi.org/10.1038/labinvest.3700603"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/j.gastro.2007.10.039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028004328"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2337/diabetes.54.10.2917", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028160301"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.str.32.2.553", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028161169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1012338828418", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030513189", 
          "https://doi.org/10.1023/a:1012338828418"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/en.2005-0570", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039961136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.7.2466", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042342506"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1194/jlr.m600299-jlr200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044491353"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.freeradbiomed.2007.05.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045817381"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1001/jama.289.22.3000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045878964"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0165-6147(98)01184-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047599244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.43.3.506", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048426100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpendo.00642.2009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053139134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuint.2010.05.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053372336"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1055/s-0028-1091980", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057110876"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1055/s-2007-978865", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057508305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci14550", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063415279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci32752", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063417015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/en.2004-0478", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064246816"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074735194", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpendo.2002.282.1.e170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074962395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079518059", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079675410", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079981956", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-12", 
    "datePublishedReg": "2011-12-01", 
    "description": "AIMS: Lipid accumulation in non-adipose tissues leads to cell dysfunction and apoptosis, a phenomenon known as lipotoxicity. Unsaturated fatty acids may offset the lipotoxicity associated with saturated fatty acids. Stearic acid induced endoplasmic reticulum (ER) stress and caused apoptotic and necrotic cell death in the primary rat hepatocytes.\nMETHODS: Cell viability was investigated using MTT assay, and apoptosis was evaluated with Hoechst 33342 staining. Western blot analysis was used to examine the changes in the expression levels of glucose regulated protein 78 (GRP78), glucose regulated protein 94 (GRP94), and C/EBP homologous protein (CHOP). Caspase-3 activity was evaluated using a Caspase-3 substrate kit.\nRESULTS: We have studied the ability of \u03b1-linolenic acid to prevent endoplasmic reticulum stress-mediated apoptosis of rat hepatocytes elicited by stearic acid and thapsigargin. Incubation of primary rat hepatocytes for 16 h with stearic acid produced a significant increase in cell death. Stearic acid also increased levels of three indicators of ER stress -- GRP78, CHOP, and GRP94. \u03b1-Linolenic acid distinctly reduced cell death and levels of all three indicators of ER stress brought about by stearic acid. Thapsigargin, which induces ER stress produced similar effects to those obtained using stearic acid; its effects were partly reversed by \u03b1-linolenic acid.\nCONCLUSION: These results suggest that \u03b1-linolenic acid prevents ER stress-mediated apoptosis of stearic acid lipotoxicity on primary rat hepatocytes might become a target to develop new antiapoptotic compounds in nonalcoholic fatty liver disease (NAFLD).", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1476-511x-10-81", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1031029", 
        "issn": [
          "1476-511X"
        ], 
        "name": "Lipids in Health and Disease", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "\u03b1-Linolenic acid prevents endoplasmic reticulum stress-mediated apoptosis of stearic acid lipotoxicity on primary rat hepatocytes", 
    "pagination": "81", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "0b41d55f4ede58bf6a569f78307be8f1080b8028ceafd6ec63c571ed94cd4c77"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21592363"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101147696"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1476-511x-10-81"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1002364320"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1476-511x-10-81", 
      "https://app.dimensions.ai/details/publication/pub.1002364320"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000509.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1476-511X-10-81"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1476-511x-10-81'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1476-511x-10-81'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1476-511x-10-81'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1476-511x-10-81'


 

This table displays all metadata directly associated to this object as RDF triples.

258 TRIPLES      21 PREDICATES      79 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1476-511x-10-81 schema:about N2f66ecbbaaeb467eb1da8bef477da5a4
2 N73da25fb48a149759b835b542a35da31
3 N8241a8e704dc4b37beb446f1ece9b514
4 N86eab279e9ef4c46ad365cd8a458e25e
5 N90b12d276086483e9b194f44f3a204be
6 N935259e585634510908971a98e58a1a5
7 N9891bb8e072b405eb84f5f0e5d329517
8 Na86a9c94c31d46a883c6dc4bcd891f56
9 Naf1581fdf6004c3e9e527e351f6f7921
10 Nc3e09ef8ff5d42e98365c808ed91c81e
11 Nc72d50fc9a944aa380c60de2edcd0c84
12 Ncf34e35099234594bf8caa0c21241125
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N823f05f9a16d47bea320bf99ef8e5eab
16 schema:citation sg:pub.10.1007/s00125-005-1861-9
17 sg:pub.10.1023/a:1012338828418
18 sg:pub.10.1038/labinvest.3700603
19 https://app.dimensions.ai/details/publication/pub.1074735194
20 https://app.dimensions.ai/details/publication/pub.1079518059
21 https://app.dimensions.ai/details/publication/pub.1079675410
22 https://app.dimensions.ai/details/publication/pub.1079981956
23 https://doi.org/10.1001/jama.289.22.3000
24 https://doi.org/10.1002/cbf.1667
25 https://doi.org/10.1006/jsre.2000.5959
26 https://doi.org/10.1016/j.freeradbiomed.2007.05.014
27 https://doi.org/10.1016/j.neuint.2010.05.006
28 https://doi.org/10.1016/s0027-5107(03)00134-9
29 https://doi.org/10.1016/s0091-679x(08)61797-5
30 https://doi.org/10.1016/s0165-6147(98)01184-5
31 https://doi.org/10.1016/s1388-1981(02)00342-6
32 https://doi.org/10.1053/j.gastro.2007.10.039
33 https://doi.org/10.1055/s-0028-1091980
34 https://doi.org/10.1055/s-2007-978865
35 https://doi.org/10.1073/pnas.0630588100
36 https://doi.org/10.1073/pnas.87.7.2466
37 https://doi.org/10.1074/jbc.m510660200
38 https://doi.org/10.1083/jcb.43.3.506
39 https://doi.org/10.1097/00003246-198907000-00020
40 https://doi.org/10.1126/science.1103160
41 https://doi.org/10.1152/ajpendo.00478.2007
42 https://doi.org/10.1152/ajpendo.00642.2009
43 https://doi.org/10.1152/ajpendo.00644.2005
44 https://doi.org/10.1152/ajpendo.2002.282.1.e170
45 https://doi.org/10.1152/ajpheart.00980.2009
46 https://doi.org/10.1161/01.str.32.2.553
47 https://doi.org/10.1172/jci14550
48 https://doi.org/10.1172/jci23621
49 https://doi.org/10.1172/jci32752
50 https://doi.org/10.1194/jlr.m600299-jlr200
51 https://doi.org/10.1210/en.2004-0478
52 https://doi.org/10.1210/en.2005-0570
53 https://doi.org/10.2337/diabetes.54.10.2917
54 schema:datePublished 2011-12
55 schema:datePublishedReg 2011-12-01
56 schema:description AIMS: Lipid accumulation in non-adipose tissues leads to cell dysfunction and apoptosis, a phenomenon known as lipotoxicity. Unsaturated fatty acids may offset the lipotoxicity associated with saturated fatty acids. Stearic acid induced endoplasmic reticulum (ER) stress and caused apoptotic and necrotic cell death in the primary rat hepatocytes. METHODS: Cell viability was investigated using MTT assay, and apoptosis was evaluated with Hoechst 33342 staining. Western blot analysis was used to examine the changes in the expression levels of glucose regulated protein 78 (GRP78), glucose regulated protein 94 (GRP94), and C/EBP homologous protein (CHOP). Caspase-3 activity was evaluated using a Caspase-3 substrate kit. RESULTS: We have studied the ability of α-linolenic acid to prevent endoplasmic reticulum stress-mediated apoptosis of rat hepatocytes elicited by stearic acid and thapsigargin. Incubation of primary rat hepatocytes for 16 h with stearic acid produced a significant increase in cell death. Stearic acid also increased levels of three indicators of ER stress -- GRP78, CHOP, and GRP94. α-Linolenic acid distinctly reduced cell death and levels of all three indicators of ER stress brought about by stearic acid. Thapsigargin, which induces ER stress produced similar effects to those obtained using stearic acid; its effects were partly reversed by α-linolenic acid. CONCLUSION: These results suggest that α-linolenic acid prevents ER stress-mediated apoptosis of stearic acid lipotoxicity on primary rat hepatocytes might become a target to develop new antiapoptotic compounds in nonalcoholic fatty liver disease (NAFLD).
57 schema:genre research_article
58 schema:inLanguage en
59 schema:isAccessibleForFree true
60 schema:isPartOf N79adfa25f8734c3e9ef508e0be1aeab8
61 N88c87c5c30914c34850111298b9ccc02
62 sg:journal.1031029
63 schema:name α-Linolenic acid prevents endoplasmic reticulum stress-mediated apoptosis of stearic acid lipotoxicity on primary rat hepatocytes
64 schema:pagination 81
65 schema:productId N7815cff7ac4d4937a0b163fdcf544052
66 N929218301baa43b3976ce531bafcac3c
67 Nbb904fc704c04c5a8241b2db80b1f09f
68 Nbc5241a25f184540860fb5325bfcd76f
69 Nf24432784df24aefb649c5330e2b327e
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002364320
71 https://doi.org/10.1186/1476-511x-10-81
72 schema:sdDatePublished 2019-04-10T15:00
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher Nd284507846af4bf697d4814454208edf
75 schema:url http://link.springer.com/10.1186%2F1476-511X-10-81
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N2f66ecbbaaeb467eb1da8bef477da5a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Cells, Cultured
81 rdf:type schema:DefinedTerm
82 N73da25fb48a149759b835b542a35da31 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Stearic Acids
84 rdf:type schema:DefinedTerm
85 N7815cff7ac4d4937a0b163fdcf544052 schema:name nlm_unique_id
86 schema:value 101147696
87 rdf:type schema:PropertyValue
88 N79adfa25f8734c3e9ef508e0be1aeab8 schema:issueNumber 1
89 rdf:type schema:PublicationIssue
90 N800aade5b2fe49b99d9b9087ddb843b3 rdf:first sg:person.01234403427.16
91 rdf:rest rdf:nil
92 N823f05f9a16d47bea320bf99ef8e5eab rdf:first sg:person.01131633214.12
93 rdf:rest Nad07d9560a8f413e8bac94b9588ab7dc
94 N8241a8e704dc4b37beb446f1ece9b514 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Apoptosis
96 rdf:type schema:DefinedTerm
97 N86eab279e9ef4c46ad365cd8a458e25e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Endoplasmic Reticulum
99 rdf:type schema:DefinedTerm
100 N88c87c5c30914c34850111298b9ccc02 schema:volumeNumber 10
101 rdf:type schema:PublicationVolume
102 N8a8f324d7cde4a82ba5162f9ecad2adc rdf:first sg:person.01317420371.28
103 rdf:rest N800aade5b2fe49b99d9b9087ddb843b3
104 N90b12d276086483e9b194f44f3a204be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Animals
106 rdf:type schema:DefinedTerm
107 N929218301baa43b3976ce531bafcac3c schema:name readcube_id
108 schema:value 0b41d55f4ede58bf6a569f78307be8f1080b8028ceafd6ec63c571ed94cd4c77
109 rdf:type schema:PropertyValue
110 N935259e585634510908971a98e58a1a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Rats
112 rdf:type schema:DefinedTerm
113 N9891bb8e072b405eb84f5f0e5d329517 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Rats, Sprague-Dawley
115 rdf:type schema:DefinedTerm
116 Na86a9c94c31d46a883c6dc4bcd891f56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Thapsigargin
118 rdf:type schema:DefinedTerm
119 Nad07d9560a8f413e8bac94b9588ab7dc rdf:first sg:person.01122713516.35
120 rdf:rest Nc565291d71e7491697ea0d432c076590
121 Naf1581fdf6004c3e9e527e351f6f7921 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Hepatocytes
123 rdf:type schema:DefinedTerm
124 Nbb904fc704c04c5a8241b2db80b1f09f schema:name pubmed_id
125 schema:value 21592363
126 rdf:type schema:PropertyValue
127 Nbc5241a25f184540860fb5325bfcd76f schema:name dimensions_id
128 schema:value pub.1002364320
129 rdf:type schema:PropertyValue
130 Nc3e09ef8ff5d42e98365c808ed91c81e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Protective Agents
132 rdf:type schema:DefinedTerm
133 Nc565291d71e7491697ea0d432c076590 rdf:first sg:person.01246061614.06
134 rdf:rest N8a8f324d7cde4a82ba5162f9ecad2adc
135 Nc72d50fc9a944aa380c60de2edcd0c84 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Stress, Physiological
137 rdf:type schema:DefinedTerm
138 Ncf34e35099234594bf8caa0c21241125 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name alpha-Linolenic Acid
140 rdf:type schema:DefinedTerm
141 Nd284507846af4bf697d4814454208edf schema:name Springer Nature - SN SciGraph project
142 rdf:type schema:Organization
143 Nf24432784df24aefb649c5330e2b327e schema:name doi
144 schema:value 10.1186/1476-511x-10-81
145 rdf:type schema:PropertyValue
146 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
147 schema:name Biological Sciences
148 rdf:type schema:DefinedTerm
149 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
150 schema:name Biochemistry and Cell Biology
151 rdf:type schema:DefinedTerm
152 sg:journal.1031029 schema:issn 1476-511X
153 schema:name Lipids in Health and Disease
154 rdf:type schema:Periodical
155 sg:person.01122713516.35 schema:affiliation https://www.grid.ac/institutes/grid.452672.0
156 schema:familyName Dong
157 schema:givenName Lei
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122713516.35
159 rdf:type schema:Person
160 sg:person.01131633214.12 schema:affiliation https://www.grid.ac/institutes/grid.452672.0
161 schema:familyName Zhang
162 schema:givenName Yong
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131633214.12
164 rdf:type schema:Person
165 sg:person.01234403427.16 schema:affiliation https://www.grid.ac/institutes/grid.452672.0
166 schema:familyName Zhang
167 schema:givenName Li
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234403427.16
169 rdf:type schema:Person
170 sg:person.01246061614.06 schema:affiliation https://www.grid.ac/institutes/grid.452672.0
171 schema:familyName Yang
172 schema:givenName Xia
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01246061614.06
174 rdf:type schema:Person
175 sg:person.01317420371.28 schema:affiliation https://www.grid.ac/institutes/grid.452672.0
176 schema:familyName Shi
177 schema:givenName Hongyang
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317420371.28
179 rdf:type schema:Person
180 sg:pub.10.1007/s00125-005-1861-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022928175
181 https://doi.org/10.1007/s00125-005-1861-9
182 rdf:type schema:CreativeWork
183 sg:pub.10.1023/a:1012338828418 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030513189
184 https://doi.org/10.1023/a:1012338828418
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/labinvest.3700603 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026270369
187 https://doi.org/10.1038/labinvest.3700603
188 rdf:type schema:CreativeWork
189 https://app.dimensions.ai/details/publication/pub.1074735194 schema:CreativeWork
190 https://app.dimensions.ai/details/publication/pub.1079518059 schema:CreativeWork
191 https://app.dimensions.ai/details/publication/pub.1079675410 schema:CreativeWork
192 https://app.dimensions.ai/details/publication/pub.1079981956 schema:CreativeWork
193 https://doi.org/10.1001/jama.289.22.3000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045878964
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1002/cbf.1667 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004420059
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1006/jsre.2000.5959 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000528624
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/j.freeradbiomed.2007.05.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045817381
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/j.neuint.2010.05.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053372336
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/s0027-5107(03)00134-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007123807
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/s0091-679x(08)61797-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018711343
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/s0165-6147(98)01184-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047599244
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/s1388-1981(02)00342-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008295160
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1053/j.gastro.2007.10.039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028004328
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1055/s-0028-1091980 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057110876
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1055/s-2007-978865 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057508305
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1073/pnas.0630588100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017297859
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1073/pnas.87.7.2466 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042342506
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1074/jbc.m510660200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009663644
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1083/jcb.43.3.506 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048426100
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1097/00003246-198907000-00020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026048686
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1126/science.1103160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025886111
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1152/ajpendo.00478.2007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009556678
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1152/ajpendo.00642.2009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053139134
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1152/ajpendo.00644.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024855324
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1152/ajpendo.2002.282.1.e170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074962395
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1152/ajpheart.00980.2009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025091789
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1161/01.str.32.2.553 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028161169
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1172/jci14550 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063415279
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1172/jci23621 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021475977
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1172/jci32752 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063417015
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1194/jlr.m600299-jlr200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044491353
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1210/en.2004-0478 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064246816
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1210/en.2005-0570 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039961136
252 rdf:type schema:CreativeWork
253 https://doi.org/10.2337/diabetes.54.10.2917 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028160301
254 rdf:type schema:CreativeWork
255 https://www.grid.ac/institutes/grid.452672.0 schema:alternateName Second Affiliated Hospital of Xi'an Jiaotong University
256 schema:name Department of Gastroenterology, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China
257 Department of Respiration, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China
258 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...