Regulation of the stem cell marker CD133 is independent of promoter hypermethylation in human epithelial differentiation and cancer View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-12

AUTHORS

Davide Pellacani, Richard J Packer, Fiona M Frame, Emma E Oldridge, Paul A Berry, Marie-Christine Labarthe, Michael J Stower, Matthew S Simms, Anne T Collins, Norman J Maitland

ABSTRACT

BACKGROUND: Epigenetic control is essential for maintenance of tissue hierarchy and correct differentiation. In cancer, this hierarchical structure is altered and epigenetic control deregulated, but the relationship between these two phenomena is still unclear. CD133 is a marker for adult stem cells in various tissues and tumour types. Stem cell specificity is maintained by tight regulation of CD133 expression at both transcriptional and post-translational levels. In this study we investigated the role of epigenetic regulation of CD133 in epithelial differentiation and cancer. METHODS: DNA methylation analysis of the CD133 promoter was done by pyrosequencing and methylation specific PCR; qRT-PCR was used to measure CD133 expression and chromatin structure was determined by ChIP. Cells were treated with DNA demethylating agents and HDAC inhibitors. All the experiments were carried out in both cell lines and primary samples. RESULTS: We found that CD133 expression is repressed by DNA methylation in the majority of prostate epithelial cell lines examined, where the promoter is heavily CpG hypermethylated, whereas in primary prostate cancer and benign prostatic hyperplasia, low levels of DNA methylation, accompanied by low levels of mRNA, were found. Moreover, differential methylation of CD133 was absent from both benign or malignant CD133+/α2β1integrinhi prostate (stem) cells, when compared to CD133-/α2β1integrinhi (transit amplifying) cells or CD133-/α2β1integrinlow (basal committed) cells, selected from primary epithelial cultures. Condensed chromatin was associated with CD133 downregulation in all of the cell lines, and treatment with HDAC inhibitors resulted in CD133 re-expression in both cell lines and primary samples. CONCLUSIONS: CD133 is tightly regulated by DNA methylation only in cell lines, where promoter methylation and gene expression inversely correlate. This highlights the crucial choice of cell model systems when studying epigenetic control in cancer biology and stem cell biology. Significantly, in both benign and malignant prostate primary tissues, regulation of CD133 is independent of DNA methylation, but is under the dynamic control of chromatin condensation. This indicates that CD133 expression is not altered in prostate cancer and it is consistent with an important role for CD133 in the maintenance of the hierarchical cell differentiation patterns in cancer. More... »

PAGES

94

References to SciGraph publications

  • 2010-03. Consolidation of the cancer genome into domains of repressive chromatin by long-range epigenetic silencing (LRES) reduces transcriptional plasticity in NATURE CELL BIOLOGY
  • 2008-06. Gene silencing in cancer by histone H3 lysine 27 trimethylation independent of promoter DNA methylation in NATURE GENETICS
  • 2006-12. Analysis of gene expression and chemoresistance of CD133+ cancer stem cells in glioblastoma in MOLECULAR CANCER
  • 2007-01. Identification and expansion of human colon-cancer-initiating cells in NATURE
  • 2008-05. Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions in GENOME BIOLOGY
  • 2005-01. Prominin-1/CD133, a neural and hematopoietic stem cell marker, is expressed in adult human differentiated cells and certain types of kidney cancer in CELL AND TISSUE RESEARCH
  • 2008-08. Genome-scale DNA methylation maps of pluripotent and differentiated cells in NATURE
  • 2003-04-17. Prostate Epithelial Stem Cell Isolation and Culture in PROSTATE CANCER METHODS AND PROTOCOLS
  • 2007-01. A human colon cancer cell capable of initiating tumour growth in immunodeficient mice in NATURE
  • 2006-01. The epigenetic progenitor origin of human cancer in NATURE REVIEWS GENETICS
  • 2004-11. Identification of human brain tumour initiating cells in NATURE
  • 2008-12-11. Generation of a prostate from a single adult stem cell in NATURE
  • 2006-12. Glioma stem cells promote radioresistance by preferential activation of the DNA damage response in NATURE
  • 2011-01. CD133 suppresses neuroblastoma cell differentiation via signal pathway modification in ONCOGENE
  • 2009-11. DNA methylation protects hematopoietic stem cell multipotency from myeloerythroid restriction in NATURE GENETICS
  • 2006-02. Epigenetic gene silencing in cancer – a mechanism for early oncogenic pathway addiction? in NATURE REVIEWS CANCER
  • 2005-04. Tumour stem cells and drug resistance in NATURE REVIEWS CANCER
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1476-4598-10-94

    DOI

    http://dx.doi.org/10.1186/1476-4598-10-94

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1052268947

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21801380


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