Anti-erythropoietin antibody levels and its association with anaemia in different strains of semi-immune mice infected with Plasmodium berghei ANKA View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-12

AUTHORS

Gideon Kofi Helegbe, Nguyen Tien Huy, Tetsuo Yanagi, Mohammed Nasir Shuaibu, Mihoko Kikuchi, Mahamoud Sama Cherif, Kenji Hirayama

ABSTRACT

BACKGROUND: Malaria anaemia is still a major public health problem and its pathogenesis still unclear. Interestingly, the progression of anaemia is at relatively low parasitaemia with some mortality in the semi-immune individuals in the endemic areas despite adequate erythropoietin (EPO) synthesis. A recent study has shown that treatment with exogenous anti-erythropoietin (anti-EPO) antibodies (Ab) of infected mice gives protection against malaria infection, suggesting an important role for anti-EPO Ab in malaria. The objective of the study was to evaluate anti-EPO antibody levels in anaemic condition of different strains of semi-immune mice with malaria. METHODOLOGY: Semi-immune status was attained in four mice strains (Balb/c, B6, CBA and NZW) by repeated infections with 10⁴Plasmodium berghei ANKA, and treatment with chloroquine/pyrimethamine. ELISA was used to measure anti-EPO Ab, transferrin and EPO while inflammatory cytokines measurement was done using bead-based multiplex assay kit. RESULTS: The mean anti-EPO Ab levels in the mice strains [Optical Density (OD) values at 450 nm: Balb/c (2.1); B6 (1.3); CBA (1.4) and NZW (1.7)] differed (p = 0.045), and were significantly higher when compared with uninfected controls, p < 0.0001, and mean anti-EPO Ab levels in the mice strains at recovery [OD values at 450 nm: Balb/c (1.8); B6 (1.1); CBA (1.5) and NZW (1.0) also differed (p = 0.0004). Interestingly, EPO levels were significantly high in NZW and low in Balb/c mice (p < 0.05), with those of B6 and CBA of intermediary values. Again, NZW were highly parasitaemic (20.7%) and the other strains (Balb/c, B6 and CBA) ranged between 2.2-2.8% (p = 0.015). Anti-EPO Ab correlated positively with extent of Hb loss (r = 0.5861; p = 0.003). Correlation of anti-EPO antibody with EPO was significant only in Balb/c mice (r = -0.83; p = 0.01). Significant levels of IL6 and IFNγ (p < 0.0001), both known to be associated with erythropoiesis suppression were observed in the Balb/c. Transferrin was significantly lower in Balb/c (p < 0.0001) when compared with the other mice strains (B6, CBA and NZW). CONCLUSION: This is the first ever report in estimating endogenous anti-EPO antibodies in malaria anaemia. The data presented here suggest that anti-EPO Ab is produced at infection and is associated with Hb loss. Host factors appear to influence anti-EPO antibody levels in the different strains of mice. More... »

PAGES

296

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1475-2875-12-296

DOI

http://dx.doi.org/10.1186/1475-2875-12-296

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045421480

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23978045


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anemia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Autoantibodies", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Erythropoietin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malaria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmodium berghei", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University for Development Studies", 
          "id": "https://www.grid.ac/institutes/grid.442305.4", 
          "name": [
            "Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan", 
            "Department of Biochemistry and Molecular Medicine, School of Medicine and Health Sciences, University for Development Studies, Tamale, Ghana"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Helegbe", 
        "givenName": "Gideon Kofi", 
        "id": "sg:person.01205321044.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205321044.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagasaki University", 
          "id": "https://www.grid.ac/institutes/grid.174567.6", 
          "name": [
            "Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huy", 
        "givenName": "Nguyen Tien", 
        "id": "sg:person.01123645113.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123645113.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagasaki University", 
          "id": "https://www.grid.ac/institutes/grid.174567.6", 
          "name": [
            "Animal Research Centre for Tropical Infections, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yanagi", 
        "givenName": "Tetsuo", 
        "id": "sg:person.013641516521.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013641516521.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagasaki University", 
          "id": "https://www.grid.ac/institutes/grid.174567.6", 
          "name": [
            "Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan", 
            "Global Center of Excellence, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shuaibu", 
        "givenName": "Mohammed Nasir", 
        "id": "sg:person.0706652043.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706652043.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagasaki University", 
          "id": "https://www.grid.ac/institutes/grid.174567.6", 
          "name": [
            "Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kikuchi", 
        "givenName": "Mihoko", 
        "id": "sg:person.01147347114.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147347114.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagasaki University", 
          "id": "https://www.grid.ac/institutes/grid.174567.6", 
          "name": [
            "Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cherif", 
        "givenName": "Mahamoud Sama", 
        "id": "sg:person.01370107542.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370107542.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagasaki University", 
          "id": "https://www.grid.ac/institutes/grid.174567.6", 
          "name": [
            "Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan", 
            "Global Center of Excellence, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hirayama", 
        "givenName": "Kenji", 
        "id": "sg:person.0761010404.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761010404.23"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1371/journal.pone.0018909", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002616102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.97.19.10526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003173167"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa011931", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003176759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/cid/cis010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004071620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbagen.2011.09.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005377267"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/j.1440-1711.2005.01385.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006347394", 
          "https://doi.org/10.1111/j.1440-1711.2005.01385.x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4894(92)90219-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006734758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-1759(02)00012-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007058944"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/381458", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016749756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1503/cmaj.071131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017778994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1503/cmaj.071131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017778994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2141.1997.82654.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024697610"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2141.1997.82654.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024697610"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2141.2009.07664.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026197661"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2141.2009.07664.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026197661"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/cid/cis020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027074181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0169-4758(00)01774-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027360958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2875-8-91", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031580310", 
          "https://doi.org/10.1186/1475-2875-8-91"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.1780401216", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033947019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm199603073341004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034897047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2007.04976.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035478124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jinf.2009.12.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036596661"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cr.2007.35", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039463723", 
          "https://doi.org/10.1038/cr.2007.35"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2875-7-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041614093", 
          "https://doi.org/10.1186/1475-2875-7-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/314548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042796533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molimm.2008.07.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049682059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/ndt/gfi177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050025074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/ndt/gfi177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050025074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2005-08-3460", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050090576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1939-165x.2012.00432.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052370684"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00042560-199809010-00004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053528564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00042560-199809010-00004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053528564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/187152808784165162", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069222573"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074600733", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077788781", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.bmb.a071751", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082124970"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-12", 
    "datePublishedReg": "2013-12-01", 
    "description": "BACKGROUND: Malaria anaemia is still a major public health problem and its pathogenesis still unclear. Interestingly, the progression of anaemia is at relatively low parasitaemia with some mortality in the semi-immune individuals in the endemic areas despite adequate erythropoietin (EPO) synthesis. A recent study has shown that treatment with exogenous anti-erythropoietin (anti-EPO) antibodies (Ab) of infected mice gives protection against malaria infection, suggesting an important role for anti-EPO Ab in malaria. The objective of the study was to evaluate anti-EPO antibody levels in anaemic condition of different strains of semi-immune mice with malaria.\nMETHODOLOGY: Semi-immune status was attained in four mice strains (Balb/c, B6, CBA and NZW) by repeated infections with 10\u2074Plasmodium berghei ANKA, and treatment with chloroquine/pyrimethamine. ELISA was used to measure anti-EPO Ab, transferrin and EPO while inflammatory cytokines measurement was done using bead-based multiplex assay kit.\nRESULTS: The mean anti-EPO Ab levels in the mice strains [Optical Density (OD) values at 450 nm: Balb/c (2.1); B6 (1.3); CBA (1.4) and NZW (1.7)] differed (p = 0.045), and were significantly higher when compared with uninfected controls, p < 0.0001, and mean anti-EPO Ab levels in the mice strains at recovery [OD values at 450 nm: Balb/c (1.8); B6 (1.1); CBA (1.5) and NZW (1.0) also differed (p = 0.0004). Interestingly, EPO levels were significantly high in NZW and low in Balb/c mice (p < 0.05), with those of B6 and CBA of intermediary values. Again, NZW were highly parasitaemic (20.7%) and the other strains (Balb/c, B6 and CBA) ranged between 2.2-2.8% (p = 0.015). Anti-EPO Ab correlated positively with extent of Hb loss (r = 0.5861; p = 0.003). Correlation of anti-EPO antibody with EPO was significant only in Balb/c mice (r = -0.83; p = 0.01). Significant levels of IL6 and IFN\u03b3 (p < 0.0001), both known to be associated with erythropoiesis suppression were observed in the Balb/c. Transferrin was significantly lower in Balb/c (p < 0.0001) when compared with the other mice strains (B6, CBA and NZW).\nCONCLUSION: This is the first ever report in estimating endogenous anti-EPO antibodies in malaria anaemia. The data presented here suggest that anti-EPO Ab is produced at infection and is associated with Hb loss. Host factors appear to influence anti-EPO antibody levels in the different strains of mice.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1475-2875-12-296", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1030597", 
        "issn": [
          "1475-2875"
        ], 
        "name": "Malaria Journal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "name": "Anti-erythropoietin antibody levels and its association with anaemia in different strains of semi-immune mice infected with Plasmodium berghei ANKA", 
    "pagination": "296", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d85cd056fe9ea1c93f346e7077b4ba4d0b6a33f7838190ee71db9ffb20c90641"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "23978045"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101139802"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1475-2875-12-296"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1045421480"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1475-2875-12-296", 
      "https://app.dimensions.ai/details/publication/pub.1045421480"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T01:14", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000551.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1475-2875-12-296"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-12-296'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-12-296'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-12-296'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-12-296'


 

This table displays all metadata directly associated to this object as RDF triples.

243 TRIPLES      21 PREDICATES      68 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1475-2875-12-296 schema:about N180f2fe6e0444313913e1317bed7b4f6
2 N4c52133b53dd48fda5360261976990d8
3 N69712be8cbef4e5eae661669709bb416
4 N78ae8e3ef8214e9baa53c31e721ab11c
5 N794fdac7e1a9449fbab1dacd77b0de55
6 N8b72d781397b4e68be8c19c85d1bc138
7 Nb92bbbc117304bf4a3e9e1feb30158e2
8 Nf608b29ce72f467ba22b1d42b1e6300b
9 anzsrc-for:11
10 anzsrc-for:1107
11 schema:author N1f63132959bf4c228b11ab60657d5fbd
12 schema:citation sg:pub.10.1038/cr.2007.35
13 sg:pub.10.1111/j.1440-1711.2005.01385.x
14 sg:pub.10.1186/1475-2875-7-3
15 sg:pub.10.1186/1475-2875-8-91
16 https://app.dimensions.ai/details/publication/pub.1074600733
17 https://app.dimensions.ai/details/publication/pub.1077788781
18 https://doi.org/10.1002/art.1780401216
19 https://doi.org/10.1016/0014-4894(92)90219-z
20 https://doi.org/10.1016/j.bbagen.2011.09.009
21 https://doi.org/10.1016/j.jinf.2009.12.013
22 https://doi.org/10.1016/j.molimm.2008.07.030
23 https://doi.org/10.1016/s0022-1759(02)00012-1
24 https://doi.org/10.1016/s0169-4758(00)01774-9
25 https://doi.org/10.1046/j.1365-2141.1997.82654.x
26 https://doi.org/10.1056/nejm199603073341004
27 https://doi.org/10.1056/nejmoa011931
28 https://doi.org/10.1073/pnas.97.19.10526
29 https://doi.org/10.1086/314548
30 https://doi.org/10.1086/381458
31 https://doi.org/10.1093/cid/cis010
32 https://doi.org/10.1093/cid/cis020
33 https://doi.org/10.1093/ndt/gfi177
34 https://doi.org/10.1093/oxfordjournals.bmb.a071751
35 https://doi.org/10.1097/00042560-199809010-00004
36 https://doi.org/10.1111/j.1365-2141.2009.07664.x
37 https://doi.org/10.1111/j.1471-4159.2007.04976.x
38 https://doi.org/10.1111/j.1939-165x.2012.00432.x
39 https://doi.org/10.1182/blood-2005-08-3460
40 https://doi.org/10.1371/journal.pone.0018909
41 https://doi.org/10.1503/cmaj.071131
42 https://doi.org/10.2174/187152808784165162
43 schema:datePublished 2013-12
44 schema:datePublishedReg 2013-12-01
45 schema:description BACKGROUND: Malaria anaemia is still a major public health problem and its pathogenesis still unclear. Interestingly, the progression of anaemia is at relatively low parasitaemia with some mortality in the semi-immune individuals in the endemic areas despite adequate erythropoietin (EPO) synthesis. A recent study has shown that treatment with exogenous anti-erythropoietin (anti-EPO) antibodies (Ab) of infected mice gives protection against malaria infection, suggesting an important role for anti-EPO Ab in malaria. The objective of the study was to evaluate anti-EPO antibody levels in anaemic condition of different strains of semi-immune mice with malaria. METHODOLOGY: Semi-immune status was attained in four mice strains (Balb/c, B6, CBA and NZW) by repeated infections with 10⁴Plasmodium berghei ANKA, and treatment with chloroquine/pyrimethamine. ELISA was used to measure anti-EPO Ab, transferrin and EPO while inflammatory cytokines measurement was done using bead-based multiplex assay kit. RESULTS: The mean anti-EPO Ab levels in the mice strains [Optical Density (OD) values at 450 nm: Balb/c (2.1); B6 (1.3); CBA (1.4) and NZW (1.7)] differed (p = 0.045), and were significantly higher when compared with uninfected controls, p < 0.0001, and mean anti-EPO Ab levels in the mice strains at recovery [OD values at 450 nm: Balb/c (1.8); B6 (1.1); CBA (1.5) and NZW (1.0) also differed (p = 0.0004). Interestingly, EPO levels were significantly high in NZW and low in Balb/c mice (p < 0.05), with those of B6 and CBA of intermediary values. Again, NZW were highly parasitaemic (20.7%) and the other strains (Balb/c, B6 and CBA) ranged between 2.2-2.8% (p = 0.015). Anti-EPO Ab correlated positively with extent of Hb loss (r = 0.5861; p = 0.003). Correlation of anti-EPO antibody with EPO was significant only in Balb/c mice (r = -0.83; p = 0.01). Significant levels of IL6 and IFNγ (p < 0.0001), both known to be associated with erythropoiesis suppression were observed in the Balb/c. Transferrin was significantly lower in Balb/c (p < 0.0001) when compared with the other mice strains (B6, CBA and NZW). CONCLUSION: This is the first ever report in estimating endogenous anti-EPO antibodies in malaria anaemia. The data presented here suggest that anti-EPO Ab is produced at infection and is associated with Hb loss. Host factors appear to influence anti-EPO antibody levels in the different strains of mice.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree true
49 schema:isPartOf N480b4cae0fe04f50aff5ae3e9ad2d15f
50 Nea0f63ef40ac4092af43df3064358cb3
51 sg:journal.1030597
52 schema:name Anti-erythropoietin antibody levels and its association with anaemia in different strains of semi-immune mice infected with Plasmodium berghei ANKA
53 schema:pagination 296
54 schema:productId N1f82de9e41b34e4a994af1eedbbdaf25
55 N3d1b1d0445b34b10ade8ccbe7270ed5b
56 N6c7ac8a47ec74767967c700d31b95825
57 N9d776bd5d9ff45e7970847ce80e51d05
58 Nda3fc73671ac431ba3d1b1929700a606
59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045421480
60 https://doi.org/10.1186/1475-2875-12-296
61 schema:sdDatePublished 2019-04-11T01:14
62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
63 schema:sdPublisher N1e71d73e78e642a8a2cd2bbcee07bb95
64 schema:url http://link.springer.com/10.1186%2F1475-2875-12-296
65 sgo:license sg:explorer/license/
66 sgo:sdDataset articles
67 rdf:type schema:ScholarlyArticle
68 N009fb77573ef4639a04832180e6a041f rdf:first sg:person.013641516521.10
69 rdf:rest N55e0b22679904081964e374d10cb5119
70 N180f2fe6e0444313913e1317bed7b4f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Plasmodium berghei
72 rdf:type schema:DefinedTerm
73 N1e71d73e78e642a8a2cd2bbcee07bb95 schema:name Springer Nature - SN SciGraph project
74 rdf:type schema:Organization
75 N1f63132959bf4c228b11ab60657d5fbd rdf:first sg:person.01205321044.51
76 rdf:rest Ncebbac5bc4ab43ab8d6a7e2e5da61c52
77 N1f82de9e41b34e4a994af1eedbbdaf25 schema:name nlm_unique_id
78 schema:value 101139802
79 rdf:type schema:PropertyValue
80 N3d1b1d0445b34b10ade8ccbe7270ed5b schema:name doi
81 schema:value 10.1186/1475-2875-12-296
82 rdf:type schema:PropertyValue
83 N480b4cae0fe04f50aff5ae3e9ad2d15f schema:issueNumber 1
84 rdf:type schema:PublicationIssue
85 N4c52133b53dd48fda5360261976990d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Malaria
87 rdf:type schema:DefinedTerm
88 N55e0b22679904081964e374d10cb5119 rdf:first sg:person.0706652043.71
89 rdf:rest N59e64b6fe82740b8a3ea84994c9f7891
90 N59e64b6fe82740b8a3ea84994c9f7891 rdf:first sg:person.01147347114.75
91 rdf:rest N934ebce187b24bd4a5c0a00e9f29cbdd
92 N69712be8cbef4e5eae661669709bb416 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Anemia
94 rdf:type schema:DefinedTerm
95 N6c7ac8a47ec74767967c700d31b95825 schema:name dimensions_id
96 schema:value pub.1045421480
97 rdf:type schema:PropertyValue
98 N78ae8e3ef8214e9baa53c31e721ab11c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Erythropoietin
100 rdf:type schema:DefinedTerm
101 N794fdac7e1a9449fbab1dacd77b0de55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Animals
103 rdf:type schema:DefinedTerm
104 N8b72d781397b4e68be8c19c85d1bc138 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Autoantibodies
106 rdf:type schema:DefinedTerm
107 N934ebce187b24bd4a5c0a00e9f29cbdd rdf:first sg:person.01370107542.63
108 rdf:rest N951dc7e78dcd4abcbf0fb3f3ac4af9e7
109 N951dc7e78dcd4abcbf0fb3f3ac4af9e7 rdf:first sg:person.0761010404.23
110 rdf:rest rdf:nil
111 N9d776bd5d9ff45e7970847ce80e51d05 schema:name readcube_id
112 schema:value d85cd056fe9ea1c93f346e7077b4ba4d0b6a33f7838190ee71db9ffb20c90641
113 rdf:type schema:PropertyValue
114 Nb92bbbc117304bf4a3e9e1feb30158e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Mice
116 rdf:type schema:DefinedTerm
117 Ncebbac5bc4ab43ab8d6a7e2e5da61c52 rdf:first sg:person.01123645113.46
118 rdf:rest N009fb77573ef4639a04832180e6a041f
119 Nda3fc73671ac431ba3d1b1929700a606 schema:name pubmed_id
120 schema:value 23978045
121 rdf:type schema:PropertyValue
122 Nea0f63ef40ac4092af43df3064358cb3 schema:volumeNumber 12
123 rdf:type schema:PublicationVolume
124 Nf608b29ce72f467ba22b1d42b1e6300b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Disease Models, Animal
126 rdf:type schema:DefinedTerm
127 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
128 schema:name Medical and Health Sciences
129 rdf:type schema:DefinedTerm
130 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
131 schema:name Immunology
132 rdf:type schema:DefinedTerm
133 sg:journal.1030597 schema:issn 1475-2875
134 schema:name Malaria Journal
135 rdf:type schema:Periodical
136 sg:person.01123645113.46 schema:affiliation https://www.grid.ac/institutes/grid.174567.6
137 schema:familyName Huy
138 schema:givenName Nguyen Tien
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123645113.46
140 rdf:type schema:Person
141 sg:person.01147347114.75 schema:affiliation https://www.grid.ac/institutes/grid.174567.6
142 schema:familyName Kikuchi
143 schema:givenName Mihoko
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147347114.75
145 rdf:type schema:Person
146 sg:person.01205321044.51 schema:affiliation https://www.grid.ac/institutes/grid.442305.4
147 schema:familyName Helegbe
148 schema:givenName Gideon Kofi
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205321044.51
150 rdf:type schema:Person
151 sg:person.013641516521.10 schema:affiliation https://www.grid.ac/institutes/grid.174567.6
152 schema:familyName Yanagi
153 schema:givenName Tetsuo
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013641516521.10
155 rdf:type schema:Person
156 sg:person.01370107542.63 schema:affiliation https://www.grid.ac/institutes/grid.174567.6
157 schema:familyName Cherif
158 schema:givenName Mahamoud Sama
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370107542.63
160 rdf:type schema:Person
161 sg:person.0706652043.71 schema:affiliation https://www.grid.ac/institutes/grid.174567.6
162 schema:familyName Shuaibu
163 schema:givenName Mohammed Nasir
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706652043.71
165 rdf:type schema:Person
166 sg:person.0761010404.23 schema:affiliation https://www.grid.ac/institutes/grid.174567.6
167 schema:familyName Hirayama
168 schema:givenName Kenji
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761010404.23
170 rdf:type schema:Person
171 sg:pub.10.1038/cr.2007.35 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039463723
172 https://doi.org/10.1038/cr.2007.35
173 rdf:type schema:CreativeWork
174 sg:pub.10.1111/j.1440-1711.2005.01385.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006347394
175 https://doi.org/10.1111/j.1440-1711.2005.01385.x
176 rdf:type schema:CreativeWork
177 sg:pub.10.1186/1475-2875-7-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041614093
178 https://doi.org/10.1186/1475-2875-7-3
179 rdf:type schema:CreativeWork
180 sg:pub.10.1186/1475-2875-8-91 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031580310
181 https://doi.org/10.1186/1475-2875-8-91
182 rdf:type schema:CreativeWork
183 https://app.dimensions.ai/details/publication/pub.1074600733 schema:CreativeWork
184 https://app.dimensions.ai/details/publication/pub.1077788781 schema:CreativeWork
185 https://doi.org/10.1002/art.1780401216 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033947019
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/0014-4894(92)90219-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1006734758
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/j.bbagen.2011.09.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005377267
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/j.jinf.2009.12.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036596661
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/j.molimm.2008.07.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049682059
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/s0022-1759(02)00012-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007058944
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/s0169-4758(00)01774-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027360958
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1046/j.1365-2141.1997.82654.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024697610
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1056/nejm199603073341004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034897047
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1056/nejmoa011931 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003176759
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1073/pnas.97.19.10526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003173167
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1086/314548 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042796533
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1086/381458 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016749756
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1093/cid/cis010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004071620
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1093/cid/cis020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027074181
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1093/ndt/gfi177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050025074
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1093/oxfordjournals.bmb.a071751 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082124970
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1097/00042560-199809010-00004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053528564
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1111/j.1365-2141.2009.07664.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026197661
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1111/j.1471-4159.2007.04976.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035478124
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1111/j.1939-165x.2012.00432.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1052370684
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1182/blood-2005-08-3460 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050090576
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1371/journal.pone.0018909 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002616102
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1503/cmaj.071131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017778994
232 rdf:type schema:CreativeWork
233 https://doi.org/10.2174/187152808784165162 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069222573
234 rdf:type schema:CreativeWork
235 https://www.grid.ac/institutes/grid.174567.6 schema:alternateName Nagasaki University
236 schema:name Animal Research Centre for Tropical Infections, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan
237 Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan
238 Global Center of Excellence, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan
239 rdf:type schema:Organization
240 https://www.grid.ac/institutes/grid.442305.4 schema:alternateName University for Development Studies
241 schema:name Department of Biochemistry and Molecular Medicine, School of Medicine and Health Sciences, University for Development Studies, Tamale, Ghana
242 Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan
243 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...