Molecular markers of antifolate resistance in Plasmodium falciparum isolates from Luanda, Angola View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-12

AUTHORS

Bianca E Gama, Guilhermina AL Pereira-Carvalho, Florbela JI Lutucuta Kosi, Natália K Almeida de Oliveira, Filomeno Fortes, Philip J Rosenthal, Virgílio E do Rosário, Cláudio Tadeu Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz

ABSTRACT

BACKGROUND: Plasmodium falciparum malaria remains a leading health problem in Africa and its control is seriously challenged by drug resistance. Although resistance to the sulphadoxine-pyrimethamine (SP) is widespread, this combination remains an important component of malaria control programmes as intermittent preventive therapy (IPT) for pregnant women and children. In Angola, resistance patterns have been poorly characterized, and IPT has been employed for pregnant women since 2006. The aim of this study was to assess the prevalence of key antifolate resistance mediating polymorphisms in the pfdhfr and pfdhps genes in P. falciparum samples from Angola. METHODS: Plasmodium falciparum samples collected in Luanda, in 2007, were genotyped by amplification and DNA forward and reverse sequencing of the pfdhfr and pfdhps genes. RESULTS: The most prevalent polymorphisms identified were pfdhfr 108N (100%), 51I (93%), 59R (57%) and pfdhps 437G (93%). Resistance-mediating polymorphisms in pfdhps less commonly observed in West Africa were also identified (540E in 10%, 581G in 7% of samples). CONCLUSION: This study documents an important prevalence of 4 P. falciparum polymorphisms that predicts an antifolate resistance in Luanda. Further, some samples presented additional mutations associated to high-level resistance. These results suggest that the use of SP for IPT may no longer be warranted in Angola. More... »

PAGES

248

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1475-2875-10-248

DOI

http://dx.doi.org/10.1186/1475-2875-10-248

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004333404

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21864379


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Angola", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antimalarials", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Child", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Child, Preschool", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dihydropteroate Synthase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Resistance", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Folic Acid Antagonists", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Frequency", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Markers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malaria, Falciparum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmodium falciparum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymorphism, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pregnancy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protozoan Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tetrahydrofolate Dehydrogenase", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil", 
            "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Mal\u00e1ria e Doen\u00e7as Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gama", 
        "givenName": "Bianca E", 
        "id": "sg:person.01307357647.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307357647.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil", 
            "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Mal\u00e1ria e Doen\u00e7as Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal", 
            "National Institute of Public Health, Luanda, Angola"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pereira-Carvalho", 
        "givenName": "Guilhermina AL", 
        "id": "sg:person.01122740667.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122740667.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Agostinho Neto University", 
          "id": "https://www.grid.ac/institutes/grid.442562.3", 
          "name": [
            "Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil", 
            "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Mal\u00e1ria e Doen\u00e7as Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal", 
            "Department of Biology, Universidade Agostinho Neto, Luanda, Angola"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lutucuta Kosi", 
        "givenName": "Florbela JI", 
        "id": "sg:person.010130534322.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010130534322.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil", 
            "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Mal\u00e1ria e Doen\u00e7as Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Almeida de Oliveira", 
        "givenName": "Nat\u00e1lia K", 
        "id": "sg:person.01261362206.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261362206.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Mal\u00e1ria e Doen\u00e7as Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal", 
            "National Institute of Public Health, Luanda, Angola"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fortes", 
        "givenName": "Filomeno", 
        "id": "sg:person.01003711753.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003711753.89"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, San Francisco", 
          "id": "https://www.grid.ac/institutes/grid.266102.1", 
          "name": [
            "Department of Medicine, San Francisco General Hospital, University of California San Francisco, San Francisco, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rosenthal", 
        "givenName": "Philip J", 
        "id": "sg:person.01257314627.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01257314627.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Mal\u00e1ria e Doen\u00e7as Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "do Ros\u00e1rio", 
        "givenName": "Virg\u00edlio E", 
        "id": "sg:person.01120724661.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120724661.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil", 
            "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Mal\u00e1ria e Doen\u00e7as Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Daniel-Ribeiro", 
        "givenName": "Cl\u00e1udio Tadeu", 
        "id": "sg:person.01220713203.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220713203.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil", 
            "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Mal\u00e1ria e Doen\u00e7as Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "F\u00e1tima Ferreira-da-Cruz", 
        "givenName": "Maria de", 
        "id": "sg:person.010067171043.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010067171043.79"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1086/504687", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002637513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/514159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002916171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.85.23.9109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005481775"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3156.2004.01290.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007948460"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(09)61258-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009148435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3156.2004.01217.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010800784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2875-7-87", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012466782", 
          "https://doi.org/10.1186/1475-2875-7-87"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1001/jama.297.23.2603", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016036218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pmed.1000055", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020228832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3156.2007.01904.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023497284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3156.2007.01904.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023497284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2875-10-22", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026962984", 
          "https://doi.org/10.1186/1475-2875-10-22"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/342946", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029635400"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/cmr.17.1.235-254.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033125062"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(98)24022-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034198331"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/pr.57.1.4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037940339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0004569", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038363802"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.47.4.1347-1354.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040132193"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/clpt.2009.284", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043531768", 
          "https://doi.org/10.1038/clpt.2009.284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/338566", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044250968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3156.2009.02369.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044331506"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2875-6-35", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045992235", 
          "https://doi.org/10.1186/1475-2875-6-35"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1994.00397.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051593980"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.trstmh.2004.11.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054751038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.trstmh.2004.11.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054751038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1586/eri.10.36", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068080133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4238/vol7-3x-meeting04", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072396808"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4269/ajtmh.2004.71.758", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1076991830"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-12", 
    "datePublishedReg": "2011-12-01", 
    "description": "BACKGROUND: Plasmodium falciparum malaria remains a leading health problem in Africa and its control is seriously challenged by drug resistance. Although resistance to the sulphadoxine-pyrimethamine (SP) is widespread, this combination remains an important component of malaria control programmes as intermittent preventive therapy (IPT) for pregnant women and children. In Angola, resistance patterns have been poorly characterized, and IPT has been employed for pregnant women since 2006. The aim of this study was to assess the prevalence of key antifolate resistance mediating polymorphisms in the pfdhfr and pfdhps genes in P. falciparum samples from Angola.\nMETHODS: Plasmodium falciparum samples collected in Luanda, in 2007, were genotyped by amplification and DNA forward and reverse sequencing of the pfdhfr and pfdhps genes.\nRESULTS: The most prevalent polymorphisms identified were pfdhfr 108N (100%), 51I (93%), 59R (57%) and pfdhps 437G (93%). Resistance-mediating polymorphisms in pfdhps less commonly observed in West Africa were also identified (540E in 10%, 581G in 7% of samples).\nCONCLUSION: This study documents an important prevalence of 4 P. falciparum polymorphisms that predicts an antifolate resistance in Luanda. Further, some samples presented additional mutations associated to high-level resistance. These results suggest that the use of SP for IPT may no longer be warranted in Angola.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1475-2875-10-248", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1030597", 
        "issn": [
          "1475-2875"
        ], 
        "name": "Malaria Journal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "Molecular markers of antifolate resistance in Plasmodium falciparum isolates from Luanda, Angola", 
    "pagination": "248", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "14a5f11c1a079acd920a2b54dac1ebcd70ed6f166fb2eca1e6d245c86151e37a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21864379"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101139802"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1475-2875-10-248"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1004333404"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1475-2875-10-248", 
      "https://app.dimensions.ai/details/publication/pub.1004333404"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:03", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000549.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1475-2875-10-248"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-10-248'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-10-248'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-10-248'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-10-248'


 

This table displays all metadata directly associated to this object as RDF triples.

296 TRIPLES      21 PREDICATES      75 URIs      41 LITERALS      29 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1475-2875-10-248 schema:about N15ffdd77032e40ce866ec611bdb62a36
2 N1cfbe1efff4a431cb2ade84c3682fffa
3 N26200ce01fc742dfbc1e638b09522a92
4 N2c7dc1b97bf1486ba1d38512e5758b32
5 N3d3727961f8e49358c0a8df6cdcae90f
6 N3eda2969c0eb4d4b940c5b13d241f1eb
7 N737acfdabdf8477fb78a9a2ef2787ffd
8 N84876076daac43e593e34a02b0f127bc
9 N8b7e4ca754f0438284201ed8ae3ed2e5
10 N968ae18d71304e7ebb88ab4eb1fac2b7
11 N995bb8a7d2104eb297b3a45e661af9c6
12 Na31141fdddf640459f20642ed6e462a7
13 Na6983ea3a9de4a0ba2ce23f550ac7f86
14 Na75aad9ae0664036b6b96993f00403fa
15 Na7eb76e85c8444e38a699e7137d067d7
16 Nadf07c45e7e34c4aa8cbc18e4ff65abe
17 Ncd3f3d166c5a41beb825dd160c2b889a
18 Ne6954cd144654f379e01ec7bd91a1e6b
19 Nf91391f9f3074297b064d95ea2c203c2
20 Nff2796efa3d54696b20b086f2ade1a79
21 anzsrc-for:11
22 anzsrc-for:1108
23 schema:author N9530772c5a1349db96b9cec19b03c526
24 schema:citation sg:pub.10.1038/clpt.2009.284
25 sg:pub.10.1186/1475-2875-10-22
26 sg:pub.10.1186/1475-2875-6-35
27 sg:pub.10.1186/1475-2875-7-87
28 https://doi.org/10.1001/jama.297.23.2603
29 https://doi.org/10.1016/j.trstmh.2004.11.010
30 https://doi.org/10.1016/s0140-6736(09)61258-7
31 https://doi.org/10.1016/s0140-6736(98)24022-0
32 https://doi.org/10.1073/pnas.85.23.9109
33 https://doi.org/10.1086/338566
34 https://doi.org/10.1086/342946
35 https://doi.org/10.1086/504687
36 https://doi.org/10.1086/514159
37 https://doi.org/10.1111/j.1365-3156.2004.01217.x
38 https://doi.org/10.1111/j.1365-3156.2004.01290.x
39 https://doi.org/10.1111/j.1365-3156.2007.01904.x
40 https://doi.org/10.1111/j.1365-3156.2009.02369.x
41 https://doi.org/10.1111/j.1432-1033.1994.00397.x
42 https://doi.org/10.1124/pr.57.1.4
43 https://doi.org/10.1128/aac.47.4.1347-1354.2003
44 https://doi.org/10.1128/cmr.17.1.235-254.2004
45 https://doi.org/10.1371/journal.pmed.1000055
46 https://doi.org/10.1371/journal.pone.0004569
47 https://doi.org/10.1586/eri.10.36
48 https://doi.org/10.4238/vol7-3x-meeting04
49 https://doi.org/10.4269/ajtmh.2004.71.758
50 schema:datePublished 2011-12
51 schema:datePublishedReg 2011-12-01
52 schema:description BACKGROUND: Plasmodium falciparum malaria remains a leading health problem in Africa and its control is seriously challenged by drug resistance. Although resistance to the sulphadoxine-pyrimethamine (SP) is widespread, this combination remains an important component of malaria control programmes as intermittent preventive therapy (IPT) for pregnant women and children. In Angola, resistance patterns have been poorly characterized, and IPT has been employed for pregnant women since 2006. The aim of this study was to assess the prevalence of key antifolate resistance mediating polymorphisms in the pfdhfr and pfdhps genes in P. falciparum samples from Angola. METHODS: Plasmodium falciparum samples collected in Luanda, in 2007, were genotyped by amplification and DNA forward and reverse sequencing of the pfdhfr and pfdhps genes. RESULTS: The most prevalent polymorphisms identified were pfdhfr 108N (100%), 51I (93%), 59R (57%) and pfdhps 437G (93%). Resistance-mediating polymorphisms in pfdhps less commonly observed in West Africa were also identified (540E in 10%, 581G in 7% of samples). CONCLUSION: This study documents an important prevalence of 4 P. falciparum polymorphisms that predicts an antifolate resistance in Luanda. Further, some samples presented additional mutations associated to high-level resistance. These results suggest that the use of SP for IPT may no longer be warranted in Angola.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree true
56 schema:isPartOf N19c4cddc2101476eb40add8ad7146541
57 Ne229b04fada94eeaa16ac45eeb7b5386
58 sg:journal.1030597
59 schema:name Molecular markers of antifolate resistance in Plasmodium falciparum isolates from Luanda, Angola
60 schema:pagination 248
61 schema:productId N06ae821116fa4976b392d42ca8181cba
62 N68a125f55fbd4b5cb64abb4caa83cd3d
63 N88cb4d4aead94cae92abe854e08356f4
64 Nc8d91b7fb8bc4d4590e52c18a55e1feb
65 Nd877445732c24579aa8f111340937634
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004333404
67 https://doi.org/10.1186/1475-2875-10-248
68 schema:sdDatePublished 2019-04-10T20:03
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher N7e1bd677cd40430b9a56692cbc754234
71 schema:url http://link.springer.com/10.1186%2F1475-2875-10-248
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N06ae821116fa4976b392d42ca8181cba schema:name readcube_id
76 schema:value 14a5f11c1a079acd920a2b54dac1ebcd70ed6f166fb2eca1e6d245c86151e37a
77 rdf:type schema:PropertyValue
78 N12d2c9b881c348eda084cb3969a38fca rdf:first sg:person.010067171043.79
79 rdf:rest rdf:nil
80 N15ffdd77032e40ce866ec611bdb62a36 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Female
82 rdf:type schema:DefinedTerm
83 N190f41b783f14bd5bac3bbf490f999ca rdf:first sg:person.01257314627.56
84 rdf:rest N4c08941a3bda46c3a36eab710695f2a3
85 N19c4cddc2101476eb40add8ad7146541 schema:volumeNumber 10
86 rdf:type schema:PublicationVolume
87 N1cfbe1efff4a431cb2ade84c3682fffa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Humans
89 rdf:type schema:DefinedTerm
90 N26200ce01fc742dfbc1e638b09522a92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Angola
92 rdf:type schema:DefinedTerm
93 N2c7dc1b97bf1486ba1d38512e5758b32 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Protozoan Proteins
95 rdf:type schema:DefinedTerm
96 N3d3727961f8e49358c0a8df6cdcae90f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Child
98 rdf:type schema:DefinedTerm
99 N3eda2969c0eb4d4b940c5b13d241f1eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Dihydropteroate Synthase
101 rdf:type schema:DefinedTerm
102 N4c08941a3bda46c3a36eab710695f2a3 rdf:first sg:person.01120724661.39
103 rdf:rest N9bd3931fb4a9429080a7980ebacc8c14
104 N4c089d242ecf4675b09d6544d0de4626 rdf:first sg:person.01261362206.24
105 rdf:rest N7c61ca74d6e944e4bfb331187606fa88
106 N556000838a4f4c66828b1b8d01fe55f6 rdf:first sg:person.01122740667.98
107 rdf:rest N9532a876cdbe4b9a8fa1289ae0bca5ae
108 N68a125f55fbd4b5cb64abb4caa83cd3d schema:name nlm_unique_id
109 schema:value 101139802
110 rdf:type schema:PropertyValue
111 N737acfdabdf8477fb78a9a2ef2787ffd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Sequence Analysis, DNA
113 rdf:type schema:DefinedTerm
114 N7c61ca74d6e944e4bfb331187606fa88 rdf:first sg:person.01003711753.89
115 rdf:rest N190f41b783f14bd5bac3bbf490f999ca
116 N7e1bd677cd40430b9a56692cbc754234 schema:name Springer Nature - SN SciGraph project
117 rdf:type schema:Organization
118 N84876076daac43e593e34a02b0f127bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Pregnancy
120 rdf:type schema:DefinedTerm
121 N88cb4d4aead94cae92abe854e08356f4 schema:name dimensions_id
122 schema:value pub.1004333404
123 rdf:type schema:PropertyValue
124 N8b7e4ca754f0438284201ed8ae3ed2e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Antimalarials
126 rdf:type schema:DefinedTerm
127 N9530772c5a1349db96b9cec19b03c526 rdf:first sg:person.01307357647.97
128 rdf:rest N556000838a4f4c66828b1b8d01fe55f6
129 N9532a876cdbe4b9a8fa1289ae0bca5ae rdf:first sg:person.010130534322.70
130 rdf:rest N4c089d242ecf4675b09d6544d0de4626
131 N968ae18d71304e7ebb88ab4eb1fac2b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Folic Acid Antagonists
133 rdf:type schema:DefinedTerm
134 N995bb8a7d2104eb297b3a45e661af9c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Malaria, Falciparum
136 rdf:type schema:DefinedTerm
137 N9bd3931fb4a9429080a7980ebacc8c14 rdf:first sg:person.01220713203.83
138 rdf:rest N12d2c9b881c348eda084cb3969a38fca
139 Na31141fdddf640459f20642ed6e462a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Genotype
141 rdf:type schema:DefinedTerm
142 Na6983ea3a9de4a0ba2ce23f550ac7f86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Child, Preschool
144 rdf:type schema:DefinedTerm
145 Na75aad9ae0664036b6b96993f00403fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Plasmodium falciparum
147 rdf:type schema:DefinedTerm
148 Na7eb76e85c8444e38a699e7137d067d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Drug Resistance
150 rdf:type schema:DefinedTerm
151 Nadf07c45e7e34c4aa8cbc18e4ff65abe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Genetic Markers
153 rdf:type schema:DefinedTerm
154 Nc8d91b7fb8bc4d4590e52c18a55e1feb schema:name doi
155 schema:value 10.1186/1475-2875-10-248
156 rdf:type schema:PropertyValue
157 Ncd3f3d166c5a41beb825dd160c2b889a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Gene Frequency
159 rdf:type schema:DefinedTerm
160 Nd877445732c24579aa8f111340937634 schema:name pubmed_id
161 schema:value 21864379
162 rdf:type schema:PropertyValue
163 Ne229b04fada94eeaa16ac45eeb7b5386 schema:issueNumber 1
164 rdf:type schema:PublicationIssue
165 Ne6954cd144654f379e01ec7bd91a1e6b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Polymorphism, Genetic
167 rdf:type schema:DefinedTerm
168 Nf91391f9f3074297b064d95ea2c203c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Adult
170 rdf:type schema:DefinedTerm
171 Nff2796efa3d54696b20b086f2ade1a79 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Tetrahydrofolate Dehydrogenase
173 rdf:type schema:DefinedTerm
174 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
175 schema:name Medical and Health Sciences
176 rdf:type schema:DefinedTerm
177 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
178 schema:name Medical Microbiology
179 rdf:type schema:DefinedTerm
180 sg:journal.1030597 schema:issn 1475-2875
181 schema:name Malaria Journal
182 rdf:type schema:Periodical
183 sg:person.01003711753.89 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
184 schema:familyName Fortes
185 schema:givenName Filomeno
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003711753.89
187 rdf:type schema:Person
188 sg:person.010067171043.79 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
189 schema:familyName Fátima Ferreira-da-Cruz
190 schema:givenName Maria de
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010067171043.79
192 rdf:type schema:Person
193 sg:person.010130534322.70 schema:affiliation https://www.grid.ac/institutes/grid.442562.3
194 schema:familyName Lutucuta Kosi
195 schema:givenName Florbela JI
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010130534322.70
197 rdf:type schema:Person
198 sg:person.01120724661.39 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
199 schema:familyName do Rosário
200 schema:givenName Virgílio E
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120724661.39
202 rdf:type schema:Person
203 sg:person.01122740667.98 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
204 schema:familyName Pereira-Carvalho
205 schema:givenName Guilhermina AL
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122740667.98
207 rdf:type schema:Person
208 sg:person.01220713203.83 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
209 schema:familyName Daniel-Ribeiro
210 schema:givenName Cláudio Tadeu
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220713203.83
212 rdf:type schema:Person
213 sg:person.01257314627.56 schema:affiliation https://www.grid.ac/institutes/grid.266102.1
214 schema:familyName Rosenthal
215 schema:givenName Philip J
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01257314627.56
217 rdf:type schema:Person
218 sg:person.01261362206.24 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
219 schema:familyName Almeida de Oliveira
220 schema:givenName Natália K
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261362206.24
222 rdf:type schema:Person
223 sg:person.01307357647.97 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
224 schema:familyName Gama
225 schema:givenName Bianca E
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307357647.97
227 rdf:type schema:Person
228 sg:pub.10.1038/clpt.2009.284 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043531768
229 https://doi.org/10.1038/clpt.2009.284
230 rdf:type schema:CreativeWork
231 sg:pub.10.1186/1475-2875-10-22 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026962984
232 https://doi.org/10.1186/1475-2875-10-22
233 rdf:type schema:CreativeWork
234 sg:pub.10.1186/1475-2875-6-35 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045992235
235 https://doi.org/10.1186/1475-2875-6-35
236 rdf:type schema:CreativeWork
237 sg:pub.10.1186/1475-2875-7-87 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012466782
238 https://doi.org/10.1186/1475-2875-7-87
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1001/jama.297.23.2603 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016036218
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/j.trstmh.2004.11.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054751038
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/s0140-6736(09)61258-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009148435
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/s0140-6736(98)24022-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034198331
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1073/pnas.85.23.9109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005481775
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1086/338566 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044250968
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1086/342946 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029635400
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1086/504687 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002637513
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1086/514159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002916171
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1111/j.1365-3156.2004.01217.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010800784
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1111/j.1365-3156.2004.01290.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007948460
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1111/j.1365-3156.2007.01904.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023497284
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1111/j.1365-3156.2009.02369.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044331506
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1111/j.1432-1033.1994.00397.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051593980
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1124/pr.57.1.4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037940339
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1128/aac.47.4.1347-1354.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040132193
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1128/cmr.17.1.235-254.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033125062
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1371/journal.pmed.1000055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020228832
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1371/journal.pone.0004569 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038363802
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1586/eri.10.36 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068080133
279 rdf:type schema:CreativeWork
280 https://doi.org/10.4238/vol7-3x-meeting04 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072396808
281 rdf:type schema:CreativeWork
282 https://doi.org/10.4269/ajtmh.2004.71.758 schema:sameAs https://app.dimensions.ai/details/publication/pub.1076991830
283 rdf:type schema:CreativeWork
284 https://www.grid.ac/institutes/grid.10772.33 schema:alternateName Universidade Nova de Lisboa
285 schema:name Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal
286 Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil
287 National Institute of Public Health, Luanda, Angola
288 rdf:type schema:Organization
289 https://www.grid.ac/institutes/grid.266102.1 schema:alternateName University of California, San Francisco
290 schema:name Department of Medicine, San Francisco General Hospital, University of California San Francisco, San Francisco, USA
291 rdf:type schema:Organization
292 https://www.grid.ac/institutes/grid.442562.3 schema:alternateName Agostinho Neto University
293 schema:name Department of Biology, Universidade Agostinho Neto, Luanda, Angola
294 Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal
295 Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil
296 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...