Polymorphism of Plasmodium falciparum Na+/H+ exchanger is indicative of a low in vitro quinine susceptibility in isolates from Viet Nam View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-06-14

AUTHORS

Véronique Sinou, Le Hong Quang, Stéphane Pelleau, Vu Nhu Huong, Nguyen Thu Huong, Le Minh Tai, Lionel Bertaux, Marc Desbordes, Christine Latour, Lai Quang Long, Nguyen Xuan Thanh, Daniel Parzy

ABSTRACT

BACKGROUND: The Plasmodium falciparum NA+/H+ exchanger (pfnhe1, gene PF13_0019) has recently been proposed to influence quinine (QN) susceptibility. However, its contribution to QN resistance seems to vary geographically depending on the genetic background of the parasites. Here, the role of this gene was investigated in in vitro QN susceptibility of isolates from Viet Nam. METHOD: Ninety-eight isolates were obtained from three different regions of the Binh Phuoc and Dak Nong bordering Cambodia provinces during 2006-2008. Among these, 79 were identified as monoclonal infection and were genotyped at the microsatellite pfnhe1 ms4760 locus and in vitro QN sensitivity data were obtained for 51 isolates. Parasite growth was assessed in the field using the HRP2 immunodetection assay. RESULTS: Significant associations were found between polymorphisms at pfnhe1 microsatellite ms4760 and susceptibility to QN. Isolates with two or more DNNND exhibited much lower susceptibility to QN than those harbouring zero or one DNNND repeats (median IC(50) of 682 nM versus median IC(50) of 300 nM; p = 0.0146) while isolates with one NHNDNHNNDDD repeat presented significantly reduced QN susceptibility than those who had two (median IC(50) of 704 nM versus median IC(50) of 375 nM; p < 0.01). These QNR associated genotype features were mainly due to the over representation of profile 7 among isolates (76.5%). The majority of parasites had pfcrt76T and wild-type pfmdr1 (> 95%) thus preventing analysis of associations with these mutations. Interestingly, area with the highest median QN IC(50) showed also the highest percentage of isolates carrying the pfnhe1 haplotype 7. CONCLUSIONS: The haplotype 7 which is the typical Asian profile is likely well-adapted to high drug pressure in this area and may constitute a good genetic marker to evaluate the dissemination of QNR in this part of the world. More... »

PAGES

164-164

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1475-2875-10-164

DOI

http://dx.doi.org/10.1186/1475-2875-10-164

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026269826

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21669011


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antimalarials", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Resistance", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Frequency", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Haplotypes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malaria, Falciparum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Parasitic Sensitivity Tests", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmodium falciparum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymorphism, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Quinine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sodium-Hydrogen Exchangers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vietnam", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France", 
          "id": "http://www.grid.ac/institutes/grid.5399.6", 
          "name": [
            "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sinou", 
        "givenName": "V\u00e9ronique", 
        "id": "sg:person.01111426274.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111426274.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Military Center of Preventive Medicine, Ho Chi Minh City, Vietnam", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Military Center of Preventive Medicine, Ho Chi Minh City, Vietnam"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Quang", 
        "givenName": "Le Hong", 
        "id": "sg:person.01043313074.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043313074.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France", 
          "id": "http://www.grid.ac/institutes/grid.5399.6", 
          "name": [
            "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pelleau", 
        "givenName": "St\u00e9phane", 
        "id": "sg:person.01214310221.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01214310221.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Military Center of Preventive Medicine, Ho Chi Minh City, Vietnam", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Military Center of Preventive Medicine, Ho Chi Minh City, Vietnam"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huong", 
        "givenName": "Vu Nhu", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Military Center of Drug Analysis and Research, Hanoi, Vietnam", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Military Center of Drug Analysis and Research, Hanoi, Vietnam"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huong", 
        "givenName": "Nguyen Thu", 
        "id": "sg:person.0630231032.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630231032.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Military Center of Preventive Medicine, Ho Chi Minh City, Vietnam", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Military Center of Preventive Medicine, Ho Chi Minh City, Vietnam"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tai", 
        "givenName": "Le Minh", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France", 
          "id": "http://www.grid.ac/institutes/grid.5399.6", 
          "name": [
            "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bertaux", 
        "givenName": "Lionel", 
        "id": "sg:person.01102626477.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102626477.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France", 
          "id": "http://www.grid.ac/institutes/grid.5399.6", 
          "name": [
            "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Desbordes", 
        "givenName": "Marc", 
        "id": "sg:person.01313416527.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313416527.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France", 
          "id": "http://www.grid.ac/institutes/grid.5399.6", 
          "name": [
            "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Latour", 
        "givenName": "Christine", 
        "id": "sg:person.0770766262.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770766262.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Military Center of Drug Analysis and Research, Hanoi, Vietnam", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Military Center of Drug Analysis and Research, Hanoi, Vietnam"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Long", 
        "givenName": "Lai Quang", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Military Institute of Hygiene and Epidemiology, Hanoi, Vietnam", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Military Institute of Hygiene and Epidemiology, Hanoi, Vietnam"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thanh", 
        "givenName": "Nguyen Xuan", 
        "id": "sg:person.0616576630.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616576630.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France", 
          "id": "http://www.grid.ac/institutes/grid.5399.6", 
          "name": [
            "UMR-MD3 Relations H\u00f4te-Parasite, Pharmacologie et Th\u00e9rapeutique, Universit\u00e9 de la M\u00e9diterran\u00e9e, Institut de M\u00e9decine Tropicale du Service de Sant\u00e9 des Arm\u00e9es, Antenne IRBA- Marseille, Marseille, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Parzy", 
        "givenName": "Daniel", 
        "id": "sg:person.01273373773.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273373773.25"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/1475-2875-10-37", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033131953", 
          "https://doi.org/10.1186/1475-2875-10-37"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2458-9-85", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009766812", 
          "https://doi.org/10.1186/1471-2458-9-85"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35002615", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013236786", 
          "https://doi.org/10.1038/35002615"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-06-14", 
    "datePublishedReg": "2011-06-14", 
    "description": "BACKGROUND: The Plasmodium falciparum NA+/H+ exchanger (pfnhe1, gene PF13_0019) has recently been proposed to influence quinine (QN) susceptibility. However, its contribution to QN resistance seems to vary geographically depending on the genetic background of the parasites. Here, the role of this gene was investigated in in vitro QN susceptibility of isolates from Viet Nam.\nMETHOD: Ninety-eight isolates were obtained from three different regions of the Binh Phuoc and Dak Nong bordering Cambodia provinces during 2006-2008. Among these, 79 were identified as monoclonal infection and were genotyped at the microsatellite pfnhe1 ms4760 locus and in vitro QN sensitivity data were obtained for 51 isolates. Parasite growth was assessed in the field using the HRP2 immunodetection assay.\nRESULTS: Significant associations were found between polymorphisms at pfnhe1 microsatellite ms4760 and susceptibility to QN. Isolates with two or more DNNND exhibited much lower susceptibility to QN than those harbouring zero or one DNNND repeats (median IC(50) of 682 nM versus median IC(50) of 300 nM; p = 0.0146) while isolates with one NHNDNHNNDDD repeat presented significantly reduced QN susceptibility than those who had two (median IC(50) of 704 nM versus median IC(50) of 375 nM; p < 0.01). These QNR associated genotype features were mainly due to the over representation of profile 7 among isolates (76.5%). The majority of parasites had pfcrt76T and wild-type pfmdr1 (> 95%) thus preventing analysis of associations with these mutations. Interestingly, area with the highest median QN IC(50) showed also the highest percentage of isolates carrying the pfnhe1 haplotype 7.\nCONCLUSIONS: The haplotype 7 which is the typical Asian profile is likely well-adapted to high drug pressure in this area and may constitute a good genetic marker to evaluate the dissemination of QNR in this part of the world.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/1475-2875-10-164", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1030597", 
        "issn": [
          "1475-2875"
        ], 
        "name": "Malaria Journal", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "keywords": [
      "haplotype 7", 
      "good genetic marker", 
      "wild-type Pfmdr1", 
      "Plasmodium falciparum", 
      "quinine susceptibility", 
      "DNNND repeats", 
      "majority of parasites", 
      "genetic markers", 
      "genetic background", 
      "parasite growth", 
      "genotype features", 
      "QN susceptibility", 
      "repeats", 
      "immunodetection assays", 
      "parasites", 
      "isolates", 
      "monoclonal infections", 
      "polymorphism", 
      "analysis of association", 
      "drug pressure", 
      "falciparum", 
      "QN resistance", 
      "profile 7", 
      "genes", 
      "loci", 
      "Binh Phuoc", 
      "low susceptibility", 
      "over representation", 
      "susceptibility", 
      "mutations", 
      "different regions", 
      "assays", 
      "sensitivity data", 
      "qnrS", 
      "growth", 
      "pfmdr1", 
      "high drug pressure", 
      "markers", 
      "role", 
      "high percentage", 
      "region", 
      "association", 
      "resistance", 
      "Nam", 
      "significant association", 
      "infection", 
      "profile", 
      "analysis", 
      "area", 
      "Viet Nam", 
      "majority", 
      "Nong", 
      "Qn", 
      "part", 
      "Phuoc", 
      "exchanger", 
      "Province", 
      "contribution", 
      "data", 
      "Dak Nong", 
      "features", 
      "dissemination", 
      "percentage", 
      "background", 
      "world", 
      "field", 
      "pressure", 
      "representation", 
      "NHNDNHNNDDD repeats", 
      "Cambodia provinces", 
      "microsatellite pfnhe1 ms4760 locus", 
      "pfnhe1 ms4760 locus", 
      "ms4760 locus", 
      "QN sensitivity data", 
      "HRP2 immunodetection assay", 
      "pfnhe1 microsatellite ms4760", 
      "microsatellite ms4760", 
      "ms4760", 
      "more DNNND", 
      "DNNND", 
      "pfcrt76T", 
      "highest median QN", 
      "median QN", 
      "pfnhe1 haplotype 7", 
      "typical Asian profile", 
      "Asian profile", 
      "dissemination of QNR"
    ], 
    "name": "Polymorphism of Plasmodium falciparum Na+/H+ exchanger is indicative of a low in vitro quinine susceptibility in isolates from Viet Nam", 
    "pagination": "164-164", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026269826"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1475-2875-10-164"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21669011"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1475-2875-10-164", 
      "https://app.dimensions.ai/details/publication/pub.1026269826"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:26", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_538.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/1475-2875-10-164"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-10-164'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-10-164'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-10-164'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1475-2875-10-164'


 

This table displays all metadata directly associated to this object as RDF triples.

289 TRIPLES      22 PREDICATES      128 URIs      117 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1475-2875-10-164 schema:about N0ba6954364eb49f398420374526cefa4
2 N68dc714c7d024951906dd00f71e83688
3 N6ed8170c050247b287f537185016c8b4
4 N7ac74d30c2224f4c912a36ea9d7c43a0
5 N885af0c789ec44178418b8027a508fe7
6 N903df16c24dd49cabf94bbf276cf5e41
7 Na9aca22bf5dd4f6b9f36ef93efabec21
8 Nc028af864b724558b77b3121b43c90cd
9 Nc1ae20a1813e42dcb5ca1852ce902d6e
10 Nd86adba9a2334242bbbe867e8817288a
11 Ne202f81059d6433b9b300a6bd7375f38
12 Nf30da43ac6864c75b247f0d14178d095
13 anzsrc-for:06
14 anzsrc-for:0604
15 schema:author N9be8c59ebb024358a3110c8563547eb8
16 schema:citation sg:pub.10.1038/35002615
17 sg:pub.10.1186/1471-2458-9-85
18 sg:pub.10.1186/1475-2875-10-37
19 schema:datePublished 2011-06-14
20 schema:datePublishedReg 2011-06-14
21 schema:description BACKGROUND: The Plasmodium falciparum NA+/H+ exchanger (pfnhe1, gene PF13_0019) has recently been proposed to influence quinine (QN) susceptibility. However, its contribution to QN resistance seems to vary geographically depending on the genetic background of the parasites. Here, the role of this gene was investigated in in vitro QN susceptibility of isolates from Viet Nam. METHOD: Ninety-eight isolates were obtained from three different regions of the Binh Phuoc and Dak Nong bordering Cambodia provinces during 2006-2008. Among these, 79 were identified as monoclonal infection and were genotyped at the microsatellite pfnhe1 ms4760 locus and in vitro QN sensitivity data were obtained for 51 isolates. Parasite growth was assessed in the field using the HRP2 immunodetection assay. RESULTS: Significant associations were found between polymorphisms at pfnhe1 microsatellite ms4760 and susceptibility to QN. Isolates with two or more DNNND exhibited much lower susceptibility to QN than those harbouring zero or one DNNND repeats (median IC(50) of 682 nM versus median IC(50) of 300 nM; p = 0.0146) while isolates with one NHNDNHNNDDD repeat presented significantly reduced QN susceptibility than those who had two (median IC(50) of 704 nM versus median IC(50) of 375 nM; p < 0.01). These QNR associated genotype features were mainly due to the over representation of profile 7 among isolates (76.5%). The majority of parasites had pfcrt76T and wild-type pfmdr1 (> 95%) thus preventing analysis of associations with these mutations. Interestingly, area with the highest median QN IC(50) showed also the highest percentage of isolates carrying the pfnhe1 haplotype 7. CONCLUSIONS: The haplotype 7 which is the typical Asian profile is likely well-adapted to high drug pressure in this area and may constitute a good genetic marker to evaluate the dissemination of QNR in this part of the world.
22 schema:genre article
23 schema:inLanguage en
24 schema:isAccessibleForFree true
25 schema:isPartOf N5a730f613c514f878164f9f885885288
26 N84289b4cb86e43bb958527b3b921afe7
27 sg:journal.1030597
28 schema:keywords Asian profile
29 Binh Phuoc
30 Cambodia provinces
31 DNNND
32 DNNND repeats
33 Dak Nong
34 HRP2 immunodetection assay
35 NHNDNHNNDDD repeats
36 Nam
37 Nong
38 Phuoc
39 Plasmodium falciparum
40 Province
41 QN resistance
42 QN sensitivity data
43 QN susceptibility
44 Qn
45 Viet Nam
46 analysis
47 analysis of association
48 area
49 assays
50 association
51 background
52 contribution
53 data
54 different regions
55 dissemination
56 dissemination of QNR
57 drug pressure
58 exchanger
59 falciparum
60 features
61 field
62 genes
63 genetic background
64 genetic markers
65 genotype features
66 good genetic marker
67 growth
68 haplotype 7
69 high drug pressure
70 high percentage
71 highest median QN
72 immunodetection assays
73 infection
74 isolates
75 loci
76 low susceptibility
77 majority
78 majority of parasites
79 markers
80 median QN
81 microsatellite ms4760
82 microsatellite pfnhe1 ms4760 locus
83 monoclonal infections
84 more DNNND
85 ms4760
86 ms4760 locus
87 mutations
88 over representation
89 parasite growth
90 parasites
91 part
92 percentage
93 pfcrt76T
94 pfmdr1
95 pfnhe1 haplotype 7
96 pfnhe1 microsatellite ms4760
97 pfnhe1 ms4760 locus
98 polymorphism
99 pressure
100 profile
101 profile 7
102 qnrS
103 quinine susceptibility
104 region
105 repeats
106 representation
107 resistance
108 role
109 sensitivity data
110 significant association
111 susceptibility
112 typical Asian profile
113 wild-type Pfmdr1
114 world
115 schema:name Polymorphism of Plasmodium falciparum Na+/H+ exchanger is indicative of a low in vitro quinine susceptibility in isolates from Viet Nam
116 schema:pagination 164-164
117 schema:productId N1b0ac8e36c114dfaa35451e183a8018a
118 N3f371f82ef6f4c96b8d81176f385b25e
119 Nb3480eef15d24446a8c6879c3ad52ee6
120 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026269826
121 https://doi.org/10.1186/1475-2875-10-164
122 schema:sdDatePublished 2022-01-01T18:26
123 schema:sdLicense https://scigraph.springernature.com/explorer/license/
124 schema:sdPublisher Naf6f0fffecf5467b9b319d95d5b48e34
125 schema:url https://doi.org/10.1186/1475-2875-10-164
126 sgo:license sg:explorer/license/
127 sgo:sdDataset articles
128 rdf:type schema:ScholarlyArticle
129 N0ba6954364eb49f398420374526cefa4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Polymorphism, Genetic
131 rdf:type schema:DefinedTerm
132 N15e774c3982d4f359e22af6903a61da4 rdf:first sg:person.01273373773.25
133 rdf:rest rdf:nil
134 N1b0ac8e36c114dfaa35451e183a8018a schema:name pubmed_id
135 schema:value 21669011
136 rdf:type schema:PropertyValue
137 N1b9b7629a313470e9f60040cb8caeaa8 rdf:first sg:person.0616576630.74
138 rdf:rest N15e774c3982d4f359e22af6903a61da4
139 N39e9787e4e2c4d0a80810f8ae5b9290c rdf:first sg:person.01214310221.15
140 rdf:rest Na06774794e8f4799bd4d81d67a73b370
141 N3f371f82ef6f4c96b8d81176f385b25e schema:name dimensions_id
142 schema:value pub.1026269826
143 rdf:type schema:PropertyValue
144 N5a730f613c514f878164f9f885885288 schema:volumeNumber 10
145 rdf:type schema:PublicationVolume
146 N68dc714c7d024951906dd00f71e83688 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Humans
148 rdf:type schema:DefinedTerm
149 N6a2dcb73b3464f12b68dfd3e15fae39d schema:affiliation grid-institutes:None
150 schema:familyName Long
151 schema:givenName Lai Quang
152 rdf:type schema:Person
153 N6ed8170c050247b287f537185016c8b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Vietnam
155 rdf:type schema:DefinedTerm
156 N7ac74d30c2224f4c912a36ea9d7c43a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Quinine
158 rdf:type schema:DefinedTerm
159 N84289b4cb86e43bb958527b3b921afe7 schema:issueNumber 1
160 rdf:type schema:PublicationIssue
161 N85e341b139e043dfa6a1a55ef7c09aa9 rdf:first N6a2dcb73b3464f12b68dfd3e15fae39d
162 rdf:rest N1b9b7629a313470e9f60040cb8caeaa8
163 N885af0c789ec44178418b8027a508fe7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Parasitic Sensitivity Tests
165 rdf:type schema:DefinedTerm
166 N903df16c24dd49cabf94bbf276cf5e41 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Haplotypes
168 rdf:type schema:DefinedTerm
169 N9074bc14230c4f47a7a31993ae2334db rdf:first sg:person.01313416527.18
170 rdf:rest Ncb3f5f9a205f4467b8f18e9cac59557e
171 N9be8c59ebb024358a3110c8563547eb8 rdf:first sg:person.01111426274.39
172 rdf:rest Ne15647221e7e4fe08493004f98725ab2
173 Na06774794e8f4799bd4d81d67a73b370 rdf:first Nb4bac3b4dfb247ef943b5afa4e1e6181
174 rdf:rest Nfeb142991f074f19bbc9ba2d3f413226
175 Na9aca22bf5dd4f6b9f36ef93efabec21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Malaria, Falciparum
177 rdf:type schema:DefinedTerm
178 Naae0eef252c5419a934133f6de428254 rdf:first sg:person.01102626477.63
179 rdf:rest N9074bc14230c4f47a7a31993ae2334db
180 Naf6f0fffecf5467b9b319d95d5b48e34 schema:name Springer Nature - SN SciGraph project
181 rdf:type schema:Organization
182 Nb3480eef15d24446a8c6879c3ad52ee6 schema:name doi
183 schema:value 10.1186/1475-2875-10-164
184 rdf:type schema:PropertyValue
185 Nb4bac3b4dfb247ef943b5afa4e1e6181 schema:affiliation grid-institutes:None
186 schema:familyName Huong
187 schema:givenName Vu Nhu
188 rdf:type schema:Person
189 Nc028af864b724558b77b3121b43c90cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
190 schema:name Plasmodium falciparum
191 rdf:type schema:DefinedTerm
192 Nc1ae20a1813e42dcb5ca1852ce902d6e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
193 schema:name Sodium-Hydrogen Exchangers
194 rdf:type schema:DefinedTerm
195 Ncb3f5f9a205f4467b8f18e9cac59557e rdf:first sg:person.0770766262.31
196 rdf:rest N85e341b139e043dfa6a1a55ef7c09aa9
197 Nd86adba9a2334242bbbe867e8817288a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name Gene Frequency
199 rdf:type schema:DefinedTerm
200 Ne15647221e7e4fe08493004f98725ab2 rdf:first sg:person.01043313074.92
201 rdf:rest N39e9787e4e2c4d0a80810f8ae5b9290c
202 Ne202f81059d6433b9b300a6bd7375f38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
203 schema:name Drug Resistance
204 rdf:type schema:DefinedTerm
205 Ne2aff0a8055c4b57bc67caf16bde6401 schema:affiliation grid-institutes:None
206 schema:familyName Tai
207 schema:givenName Le Minh
208 rdf:type schema:Person
209 Neb31e0e32810496f8d640a2d5c41b4e7 rdf:first Ne2aff0a8055c4b57bc67caf16bde6401
210 rdf:rest Naae0eef252c5419a934133f6de428254
211 Nf30da43ac6864c75b247f0d14178d095 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
212 schema:name Antimalarials
213 rdf:type schema:DefinedTerm
214 Nfeb142991f074f19bbc9ba2d3f413226 rdf:first sg:person.0630231032.12
215 rdf:rest Neb31e0e32810496f8d640a2d5c41b4e7
216 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
217 schema:name Biological Sciences
218 rdf:type schema:DefinedTerm
219 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
220 schema:name Genetics
221 rdf:type schema:DefinedTerm
222 sg:journal.1030597 schema:issn 1475-2875
223 schema:name Malaria Journal
224 schema:publisher Springer Nature
225 rdf:type schema:Periodical
226 sg:person.01043313074.92 schema:affiliation grid-institutes:None
227 schema:familyName Quang
228 schema:givenName Le Hong
229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043313074.92
230 rdf:type schema:Person
231 sg:person.01102626477.63 schema:affiliation grid-institutes:grid.5399.6
232 schema:familyName Bertaux
233 schema:givenName Lionel
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102626477.63
235 rdf:type schema:Person
236 sg:person.01111426274.39 schema:affiliation grid-institutes:grid.5399.6
237 schema:familyName Sinou
238 schema:givenName Véronique
239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111426274.39
240 rdf:type schema:Person
241 sg:person.01214310221.15 schema:affiliation grid-institutes:grid.5399.6
242 schema:familyName Pelleau
243 schema:givenName Stéphane
244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01214310221.15
245 rdf:type schema:Person
246 sg:person.01273373773.25 schema:affiliation grid-institutes:grid.5399.6
247 schema:familyName Parzy
248 schema:givenName Daniel
249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273373773.25
250 rdf:type schema:Person
251 sg:person.01313416527.18 schema:affiliation grid-institutes:grid.5399.6
252 schema:familyName Desbordes
253 schema:givenName Marc
254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313416527.18
255 rdf:type schema:Person
256 sg:person.0616576630.74 schema:affiliation grid-institutes:None
257 schema:familyName Thanh
258 schema:givenName Nguyen Xuan
259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616576630.74
260 rdf:type schema:Person
261 sg:person.0630231032.12 schema:affiliation grid-institutes:None
262 schema:familyName Huong
263 schema:givenName Nguyen Thu
264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630231032.12
265 rdf:type schema:Person
266 sg:person.0770766262.31 schema:affiliation grid-institutes:grid.5399.6
267 schema:familyName Latour
268 schema:givenName Christine
269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770766262.31
270 rdf:type schema:Person
271 sg:pub.10.1038/35002615 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013236786
272 https://doi.org/10.1038/35002615
273 rdf:type schema:CreativeWork
274 sg:pub.10.1186/1471-2458-9-85 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009766812
275 https://doi.org/10.1186/1471-2458-9-85
276 rdf:type schema:CreativeWork
277 sg:pub.10.1186/1475-2875-10-37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033131953
278 https://doi.org/10.1186/1475-2875-10-37
279 rdf:type schema:CreativeWork
280 grid-institutes:None schema:alternateName Military Center of Drug Analysis and Research, Hanoi, Vietnam
281 Military Center of Preventive Medicine, Ho Chi Minh City, Vietnam
282 Military Institute of Hygiene and Epidemiology, Hanoi, Vietnam
283 schema:name Military Center of Drug Analysis and Research, Hanoi, Vietnam
284 Military Center of Preventive Medicine, Ho Chi Minh City, Vietnam
285 Military Institute of Hygiene and Epidemiology, Hanoi, Vietnam
286 rdf:type schema:Organization
287 grid-institutes:grid.5399.6 schema:alternateName UMR-MD3 Relations Hôte-Parasite, Pharmacologie et Thérapeutique, Université de la Méditerranée, Institut de Médecine Tropicale du Service de Santé des Armées, Antenne IRBA- Marseille, Marseille, France
288 schema:name UMR-MD3 Relations Hôte-Parasite, Pharmacologie et Thérapeutique, Université de la Méditerranée, Institut de Médecine Tropicale du Service de Santé des Armées, Antenne IRBA- Marseille, Marseille, France
289 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...