Preservation of biomolecules in breast cancer tissue by a formalin-free histology system View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-12

AUTHORS

Mehdi Nassiri, Sharon Ramos, Hajir Zohourian, Vladimir Vincek, Azorides R Morales, Mehrdad Nadji

ABSTRACT

BACKGROUND: The potential problems associated with the use of formalin in histology, such as health hazards, degradation of RNA and cross-linking of proteins are well recognized. We describe the utilization of a formalin-free fixation and processing system for tissue detection of two important biopredictors in breast cancer - estrogen receptor and HER2 - at the RNA and protein levels. METHODS: Parallel sections of 62 cases of breast cancer were fixed in an alcohol-based molecular fixative and in formalin. Molecular fixative samples were processed by a novel formalin-free microwave-assisted processing system that preserves DNA, RNA and proteins. Formalin-fixed samples were processed using the conventional method. Estrogen receptor was assessed by immunohistochemistry and real-time PCR. HER2 was assessed by immunohistochemistry, FISH, CISH and real-time PCR. RESULTS: The immunohistochemical reaction for estrogen receptor was similar in molecular- and formalin-fixed samples (Spearman Rank R = 0.83, p < 0.05). Also HER2 result was similar to that of formalin-fixed counterparts after elimination of antigen retrieval step (Spearman Rank R = 0.84, p < 0.05). The result of HER2 amplification by FISH and CISH was identical in the molecular fixative and formalin-fixed samples; although a shorter digestion step was required when using the former fixative. Real-time PCR for both estrogen receptor and HER2 were successful in all of the molecular fixative specimens. CONCLUSION: The formalin-free tissue fixation and processing system is a practical platform for evaluation of biomolecular markers in breast cancer and it allows reliable DNA and RNA and protein studies. More... »

PAGES

1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1472-6890-8-1

DOI

http://dx.doi.org/10.1186/1472-6890-8-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036762454

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18230182


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Miami", 
          "id": "https://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Pathology, University of Miami Miller School of Medicine, Miami, Florida, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nassiri", 
        "givenName": "Mehdi", 
        "id": "sg:person.0603417126.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603417126.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Miami", 
          "id": "https://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Pathology, University of Miami Miller School of Medicine, Miami, Florida, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ramos", 
        "givenName": "Sharon", 
        "id": "sg:person.0655140114.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0655140114.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Miami", 
          "id": "https://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Pathology, University of Miami Miller School of Medicine, Miami, Florida, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zohourian", 
        "givenName": "Hajir", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Florida", 
          "id": "https://www.grid.ac/institutes/grid.15276.37", 
          "name": [
            "Department of Pathology, University of Florida, Gainesville, Florida, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vincek", 
        "givenName": "Vladimir", 
        "id": "sg:person.0776675366.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776675366.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Miami", 
          "id": "https://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Pathology, University of Miami Miller School of Medicine, Miami, Florida, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Morales", 
        "givenName": "Azorides R", 
        "id": "sg:person.0766136747.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766136747.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Miami", 
          "id": "https://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Pathology, University of Miami Miller School of Medicine, Miami, Florida, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nadji", 
        "givenName": "Mehrdad", 
        "id": "sg:person.01101263473.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101263473.14"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1309/ack8ahv01t47qr53", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003463487"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3780585", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005087436", 
          "https://doi.org/10.1038/labinvest.3780585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3780585", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005087436", 
          "https://doi.org/10.1038/labinvest.3780585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1525-1578(10)60617-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006395418"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9440(10)64864-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009297361"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00501395", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009748512", 
          "https://doi.org/10.1007/bf00501395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00501395", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009748512", 
          "https://doi.org/10.1007/bf00501395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9440(10)63985-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010647402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1097/01.lab.0000090154.55436.d1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015864419", 
          "https://doi.org/10.1097/01.lab.0000090154.55436.d1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1586/14737140.6.8.1225", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016270739"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/27.22.4436", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019725071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/labmed/14.12.767", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023256208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/jcp.2004.021618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025009925"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1309/4wv79n2ghj3x1841", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032640974"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1525-1578(10)60490-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033401988"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0079-6603(08)60099-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035160713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2353/jmoldx.2006.050105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035392356"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1179/his.2000.23.4.299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037032158"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00000478-200007000-00014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038764536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00000478-200007000-00014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038764536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1369/jhc.5a6725.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042044784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1369/jhc.5a6725.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042044784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1369/jhc.5a6725.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042044784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00403-007-0773-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045536801", 
          "https://doi.org/10.1007/s00403-007-0773-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00403-007-0773-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045536801", 
          "https://doi.org/10.1007/s00403-007-0773-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/pdm.0b013e31802f0804", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047068625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/pdm.0b013e31802f0804", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047068625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.pai.0000146544.51771.79", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047562579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.pai.0000146544.51771.79", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047562579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.pai.0000146544.51771.79", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047562579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3780023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049101021", 
          "https://doi.org/10.1038/labinvest.3780023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3780023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049101021", 
          "https://doi.org/10.1038/labinvest.3780023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1179/his.2001.24.3.173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049994474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1309/97wn-w6ux-xjwt-02h2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050170016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1263", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052273048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1263", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052273048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/002215540205000911", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063643039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/002215540205000911", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063643039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075035250", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1043/1543-2165(2007)131[18:asocco]2.0.co;2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077917948"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2008-12", 
    "datePublishedReg": "2008-12-01", 
    "description": "BACKGROUND: The potential problems associated with the use of formalin in histology, such as health hazards, degradation of RNA and cross-linking of proteins are well recognized. We describe the utilization of a formalin-free fixation and processing system for tissue detection of two important biopredictors in breast cancer - estrogen receptor and HER2 - at the RNA and protein levels.\nMETHODS: Parallel sections of 62 cases of breast cancer were fixed in an alcohol-based molecular fixative and in formalin. Molecular fixative samples were processed by a novel formalin-free microwave-assisted processing system that preserves DNA, RNA and proteins. Formalin-fixed samples were processed using the conventional method. Estrogen receptor was assessed by immunohistochemistry and real-time PCR. HER2 was assessed by immunohistochemistry, FISH, CISH and real-time PCR.\nRESULTS: The immunohistochemical reaction for estrogen receptor was similar in molecular- and formalin-fixed samples (Spearman Rank R = 0.83, p < 0.05). Also HER2 result was similar to that of formalin-fixed counterparts after elimination of antigen retrieval step (Spearman Rank R = 0.84, p < 0.05). The result of HER2 amplification by FISH and CISH was identical in the molecular fixative and formalin-fixed samples; although a shorter digestion step was required when using the former fixative. Real-time PCR for both estrogen receptor and HER2 were successful in all of the molecular fixative specimens.\nCONCLUSION: The formalin-free tissue fixation and processing system is a practical platform for evaluation of biomolecular markers in breast cancer and it allows reliable DNA and RNA and protein studies.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1472-6890-8-1", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1028422", 
        "issn": [
          "1472-6890"
        ], 
        "name": "BMC Clinical Pathology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "Preservation of biomolecules in breast cancer tissue by a formalin-free histology system", 
    "pagination": "1", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "af1f7dd497f5948ef0c8113ce8179df46ebf3b2c55b91d033f0925b36c1930d8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18230182"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101088665"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1472-6890-8-1"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036762454"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1472-6890-8-1", 
      "https://app.dimensions.ai/details/publication/pub.1036762454"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T16:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000550.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1472-6890-8-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1472-6890-8-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1472-6890-8-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1472-6890-8-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1472-6890-8-1'


 

This table displays all metadata directly associated to this object as RDF triples.

193 TRIPLES      21 PREDICATES      57 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1472-6890-8-1 schema:about anzsrc-for:11
2 anzsrc-for:1112
3 schema:author N30521b34c87a4c0ab402dc005d45e3fb
4 schema:citation sg:pub.10.1007/bf00501395
5 sg:pub.10.1007/s00403-007-0773-6
6 sg:pub.10.1038/labinvest.3780023
7 sg:pub.10.1038/labinvest.3780585
8 sg:pub.10.1097/01.lab.0000090154.55436.d1
9 https://app.dimensions.ai/details/publication/pub.1075035250
10 https://doi.org/10.1006/meth.2001.1263
11 https://doi.org/10.1016/s0002-9440(10)63985-5
12 https://doi.org/10.1016/s0002-9440(10)64864-x
13 https://doi.org/10.1016/s0079-6603(08)60099-9
14 https://doi.org/10.1016/s1525-1578(10)60490-4
15 https://doi.org/10.1016/s1525-1578(10)60617-4
16 https://doi.org/10.1043/1543-2165(2007)131[18:asocco]2.0.co;2
17 https://doi.org/10.1093/labmed/14.12.767
18 https://doi.org/10.1093/nar/27.22.4436
19 https://doi.org/10.1097/00000478-200007000-00014
20 https://doi.org/10.1097/01.pai.0000146544.51771.79
21 https://doi.org/10.1097/pdm.0b013e31802f0804
22 https://doi.org/10.1136/jcp.2004.021618
23 https://doi.org/10.1177/002215540205000911
24 https://doi.org/10.1179/his.2000.23.4.299
25 https://doi.org/10.1179/his.2001.24.3.173
26 https://doi.org/10.1309/4wv79n2ghj3x1841
27 https://doi.org/10.1309/97wn-w6ux-xjwt-02h2
28 https://doi.org/10.1309/ack8ahv01t47qr53
29 https://doi.org/10.1369/jhc.5a6725.2005
30 https://doi.org/10.1586/14737140.6.8.1225
31 https://doi.org/10.2353/jmoldx.2006.050105
32 schema:datePublished 2008-12
33 schema:datePublishedReg 2008-12-01
34 schema:description BACKGROUND: The potential problems associated with the use of formalin in histology, such as health hazards, degradation of RNA and cross-linking of proteins are well recognized. We describe the utilization of a formalin-free fixation and processing system for tissue detection of two important biopredictors in breast cancer - estrogen receptor and HER2 - at the RNA and protein levels. METHODS: Parallel sections of 62 cases of breast cancer were fixed in an alcohol-based molecular fixative and in formalin. Molecular fixative samples were processed by a novel formalin-free microwave-assisted processing system that preserves DNA, RNA and proteins. Formalin-fixed samples were processed using the conventional method. Estrogen receptor was assessed by immunohistochemistry and real-time PCR. HER2 was assessed by immunohistochemistry, FISH, CISH and real-time PCR. RESULTS: The immunohistochemical reaction for estrogen receptor was similar in molecular- and formalin-fixed samples (Spearman Rank R = 0.83, p < 0.05). Also HER2 result was similar to that of formalin-fixed counterparts after elimination of antigen retrieval step (Spearman Rank R = 0.84, p < 0.05). The result of HER2 amplification by FISH and CISH was identical in the molecular fixative and formalin-fixed samples; although a shorter digestion step was required when using the former fixative. Real-time PCR for both estrogen receptor and HER2 were successful in all of the molecular fixative specimens. CONCLUSION: The formalin-free tissue fixation and processing system is a practical platform for evaluation of biomolecular markers in breast cancer and it allows reliable DNA and RNA and protein studies.
35 schema:genre research_article
36 schema:inLanguage en
37 schema:isAccessibleForFree true
38 schema:isPartOf Nb4618dfec25d42e18f0331fea5e9224b
39 Ne83451c0288742a594df350118c87653
40 sg:journal.1028422
41 schema:name Preservation of biomolecules in breast cancer tissue by a formalin-free histology system
42 schema:pagination 1
43 schema:productId N05ca6a6cd44b45e4b2aab22aaffda83e
44 N1b16a68a8f024b34b90ccc25f463e9a6
45 N7d18cf8ecbe943cc988df932c9c484a1
46 Ndbf6bb59d89f42a79f30e8ac9e70bf67
47 Ne473c96719bb470c8e069e7a52a79a2f
48 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036762454
49 https://doi.org/10.1186/1472-6890-8-1
50 schema:sdDatePublished 2019-04-10T16:49
51 schema:sdLicense https://scigraph.springernature.com/explorer/license/
52 schema:sdPublisher N0430a1f1d6344016bb9fdb743f493506
53 schema:url http://link.springer.com/10.1186%2F1472-6890-8-1
54 sgo:license sg:explorer/license/
55 sgo:sdDataset articles
56 rdf:type schema:ScholarlyArticle
57 N01b0808730054b51a29feff367fb36b7 rdf:first sg:person.0776675366.33
58 rdf:rest N04878be69f5e42b28b00f5b4c1a358c4
59 N0430a1f1d6344016bb9fdb743f493506 schema:name Springer Nature - SN SciGraph project
60 rdf:type schema:Organization
61 N04878be69f5e42b28b00f5b4c1a358c4 rdf:first sg:person.0766136747.38
62 rdf:rest N577c8539c1c4414ca80d48719d7e4a61
63 N05ca6a6cd44b45e4b2aab22aaffda83e schema:name doi
64 schema:value 10.1186/1472-6890-8-1
65 rdf:type schema:PropertyValue
66 N1b16a68a8f024b34b90ccc25f463e9a6 schema:name nlm_unique_id
67 schema:value 101088665
68 rdf:type schema:PropertyValue
69 N2a1c13fc153c467c9d7a0435342fdace schema:affiliation https://www.grid.ac/institutes/grid.26790.3a
70 schema:familyName Zohourian
71 schema:givenName Hajir
72 rdf:type schema:Person
73 N30521b34c87a4c0ab402dc005d45e3fb rdf:first sg:person.0603417126.83
74 rdf:rest Nb2bf5be293b7417197cab7021901e07d
75 N577c8539c1c4414ca80d48719d7e4a61 rdf:first sg:person.01101263473.14
76 rdf:rest rdf:nil
77 N7d18cf8ecbe943cc988df932c9c484a1 schema:name pubmed_id
78 schema:value 18230182
79 rdf:type schema:PropertyValue
80 Nb2bf5be293b7417197cab7021901e07d rdf:first sg:person.0655140114.31
81 rdf:rest Nb5bb4cf91d1b4cbdb10d313fe2e882ad
82 Nb4618dfec25d42e18f0331fea5e9224b schema:issueNumber 1
83 rdf:type schema:PublicationIssue
84 Nb5bb4cf91d1b4cbdb10d313fe2e882ad rdf:first N2a1c13fc153c467c9d7a0435342fdace
85 rdf:rest N01b0808730054b51a29feff367fb36b7
86 Ndbf6bb59d89f42a79f30e8ac9e70bf67 schema:name dimensions_id
87 schema:value pub.1036762454
88 rdf:type schema:PropertyValue
89 Ne473c96719bb470c8e069e7a52a79a2f schema:name readcube_id
90 schema:value af1f7dd497f5948ef0c8113ce8179df46ebf3b2c55b91d033f0925b36c1930d8
91 rdf:type schema:PropertyValue
92 Ne83451c0288742a594df350118c87653 schema:volumeNumber 8
93 rdf:type schema:PublicationVolume
94 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
95 schema:name Medical and Health Sciences
96 rdf:type schema:DefinedTerm
97 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
98 schema:name Oncology and Carcinogenesis
99 rdf:type schema:DefinedTerm
100 sg:journal.1028422 schema:issn 1472-6890
101 schema:name BMC Clinical Pathology
102 rdf:type schema:Periodical
103 sg:person.01101263473.14 schema:affiliation https://www.grid.ac/institutes/grid.26790.3a
104 schema:familyName Nadji
105 schema:givenName Mehrdad
106 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101263473.14
107 rdf:type schema:Person
108 sg:person.0603417126.83 schema:affiliation https://www.grid.ac/institutes/grid.26790.3a
109 schema:familyName Nassiri
110 schema:givenName Mehdi
111 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603417126.83
112 rdf:type schema:Person
113 sg:person.0655140114.31 schema:affiliation https://www.grid.ac/institutes/grid.26790.3a
114 schema:familyName Ramos
115 schema:givenName Sharon
116 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0655140114.31
117 rdf:type schema:Person
118 sg:person.0766136747.38 schema:affiliation https://www.grid.ac/institutes/grid.26790.3a
119 schema:familyName Morales
120 schema:givenName Azorides R
121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766136747.38
122 rdf:type schema:Person
123 sg:person.0776675366.33 schema:affiliation https://www.grid.ac/institutes/grid.15276.37
124 schema:familyName Vincek
125 schema:givenName Vladimir
126 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776675366.33
127 rdf:type schema:Person
128 sg:pub.10.1007/bf00501395 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009748512
129 https://doi.org/10.1007/bf00501395
130 rdf:type schema:CreativeWork
131 sg:pub.10.1007/s00403-007-0773-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045536801
132 https://doi.org/10.1007/s00403-007-0773-6
133 rdf:type schema:CreativeWork
134 sg:pub.10.1038/labinvest.3780023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049101021
135 https://doi.org/10.1038/labinvest.3780023
136 rdf:type schema:CreativeWork
137 sg:pub.10.1038/labinvest.3780585 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005087436
138 https://doi.org/10.1038/labinvest.3780585
139 rdf:type schema:CreativeWork
140 sg:pub.10.1097/01.lab.0000090154.55436.d1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015864419
141 https://doi.org/10.1097/01.lab.0000090154.55436.d1
142 rdf:type schema:CreativeWork
143 https://app.dimensions.ai/details/publication/pub.1075035250 schema:CreativeWork
144 https://doi.org/10.1006/meth.2001.1263 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052273048
145 rdf:type schema:CreativeWork
146 https://doi.org/10.1016/s0002-9440(10)63985-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010647402
147 rdf:type schema:CreativeWork
148 https://doi.org/10.1016/s0002-9440(10)64864-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009297361
149 rdf:type schema:CreativeWork
150 https://doi.org/10.1016/s0079-6603(08)60099-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035160713
151 rdf:type schema:CreativeWork
152 https://doi.org/10.1016/s1525-1578(10)60490-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033401988
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1016/s1525-1578(10)60617-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006395418
155 rdf:type schema:CreativeWork
156 https://doi.org/10.1043/1543-2165(2007)131[18:asocco]2.0.co;2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077917948
157 rdf:type schema:CreativeWork
158 https://doi.org/10.1093/labmed/14.12.767 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023256208
159 rdf:type schema:CreativeWork
160 https://doi.org/10.1093/nar/27.22.4436 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019725071
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1097/00000478-200007000-00014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038764536
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1097/01.pai.0000146544.51771.79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047562579
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1097/pdm.0b013e31802f0804 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047068625
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1136/jcp.2004.021618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025009925
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1177/002215540205000911 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063643039
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1179/his.2000.23.4.299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037032158
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1179/his.2001.24.3.173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049994474
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1309/4wv79n2ghj3x1841 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032640974
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1309/97wn-w6ux-xjwt-02h2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050170016
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1309/ack8ahv01t47qr53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003463487
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1369/jhc.5a6725.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042044784
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1586/14737140.6.8.1225 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016270739
185 rdf:type schema:CreativeWork
186 https://doi.org/10.2353/jmoldx.2006.050105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035392356
187 rdf:type schema:CreativeWork
188 https://www.grid.ac/institutes/grid.15276.37 schema:alternateName University of Florida
189 schema:name Department of Pathology, University of Florida, Gainesville, Florida, USA
190 rdf:type schema:Organization
191 https://www.grid.ac/institutes/grid.26790.3a schema:alternateName University of Miami
192 schema:name Department of Pathology, University of Miami Miller School of Medicine, Miami, Florida, USA
193 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...