Structure and characterization of a novel chicken biotin-binding protein A (BBP-A) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2007-12

AUTHORS

Vesa P Hytönen, Juha AE Määttä, Einari A Niskanen, Juhani Huuskonen, Kaisa J Helttunen, Katrin K Halling, Henri R Nordlund, Kari Rissanen, Mark S Johnson, Tiina A Salminen, Markku S Kulomaa, Olli H Laitinen, Tomi T Airenne

ABSTRACT

BACKGROUND: The chicken genome contains a BBP-A gene showing similar characteristics to avidin family genes. In a previous study we reported that the BBP-A gene may encode a biotin-binding protein due to the high sequence similarity with chicken avidin, especially at regions encoding residues known to be located at the ligand-binding site of avidin. RESULTS: Here, we expand the repertoire of known macromolecular biotin binders by reporting a novel biotin-binding protein A (BBP-A) from chicken. The BBP-A recombinant protein was expressed using two different expression systems and purified with affinity chromatography, biochemically characterized and two X-ray structures were solved - in complex with D-biotin (BTN) and in complex with D-biotin D-sulfoxide (BSO). The BBP-A protein binds free biotin with high, "streptavidin-like" affinity (Kd ~ 10-13 M), which is about 50 times lower than that of chicken avidin. Surprisingly, the affinity of BBP-A for BSO is even higher than the affinity for BTN. Furthermore, the solved structures of the BBP-A--BTN and BBP-A--BSO complexes, which share the fold with the members of the avidin and lipocalin protein families, are extremely similar to each other. CONCLUSION: BBP-A is an avidin-like protein having a beta-barrel fold and high affinity towards BTN. However, BBP-A differs from the other known members of the avidin protein family in thermal stability and immunological properties. BBP-A also has a unique ligand-binding property, the ability to bind BTN and BSO at comparable affinities. BBP-A may have use as a novel material in, e.g. modern bio(nano)technological applications. More... »

PAGES

8

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1472-6807-7-8

DOI

http://dx.doi.org/10.1186/1472-6807-7-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011667021

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17343730


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