Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-12

AUTHORS

Harshita Sharma, Yasunori Kinoshita, Seiichi Fujiu, Shota Nomura, Mizuho Sawada, Shamim Ahmed, Masaki Shibuya, Kosaku Shirai, Syota Takamatsu, Tsuyoshi Watanabe, Hitoshi Yamazaki, Ryohei Kamiyama, Tetsuya Kobayashi, Hidenao Arai, Miho Suzuki, Naoto Nemoto, Ki Ando, Hidekazu Uchida, Koichiro Kitamura, Osamu Takei, Koichi Nishigaki

ABSTRACT

BACKGROUND: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip. RESULTS: In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications. CONCLUSIONS: The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies. More... »

PAGES

78

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1472-6750-14-78

DOI

http://dx.doi.org/10.1186/1472-6750-14-78

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047835231

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25141858


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