RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-12

AUTHORS

Joseph R Dobosy, Scott D Rose, Kristin R Beltz, Susan M Rupp, Kristy M Powers, Mark A Behlke, Joseph A Walder

ABSTRACT

BACKGROUND: The polymerase chain reaction (PCR) is commonly used to detect the presence of nucleic acid sequences both in research and diagnostic settings. While high specificity is often achieved, biological requirements sometimes necessitate that primers are placed in suboptimal locations which lead to problems with the formation of primer dimers and/or misamplification of homologous sequences. RESULTS: Pyrococcus abyssi (P.a.) RNase H2 was used to enable PCR to be performed using blocked primers containing a single ribonucleotide residue which are activated via cleavage by the enzyme (rhPCR). Cleavage occurs 5'-to the RNA base following primer hybridization to the target DNA. The requirement of the primer to first hybridize with the target sequence to gain activity eliminates the formation of primer-dimers and greatly reduces misamplification of closely related sequences. Mismatches near the scissile linkage decrease the efficiency of cleavage by RNase H2, further increasing the specificity of the assay. When applied to the detection of single nucleotide polymorphisms (SNPs), rhPCR was found to be far more sensitive than standard allele-specific PCR. In general, the best discrimination occurs when the mismatch is placed at the RNA:DNA base pair. CONCLUSION: rhPCR eliminates the formation of primer dimers and markedly improves the specificity of PCR with respect to off-target amplification. These advantages of the assay should find utility in challenging qPCR applications such as genotyping, high level multiplex assays and rare allele detection. More... »

PAGES

80

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1472-6750-11-80

DOI

http://dx.doi.org/10.1186/1472-6750-11-80

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1025374506

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21831278


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Primers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzyme Stability", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Hybridization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymorphism, Single Nucleotide", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pyrococcus abyssi", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ribonuclease H", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sensitivity and Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Integrated DNA Technologies (United States)", 
          "id": "https://www.grid.ac/institutes/grid.420360.3", 
          "name": [
            "Integrated DNA Technologies, Inc., 1710 Commercial Park, 5224, Coralville, IA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dobosy", 
        "givenName": "Joseph R", 
        "id": "sg:person.011502316477.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011502316477.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Integrated DNA Technologies (United States)", 
          "id": "https://www.grid.ac/institutes/grid.420360.3", 
          "name": [
            "Integrated DNA Technologies, Inc., 1710 Commercial Park, 5224, Coralville, IA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rose", 
        "givenName": "Scott D", 
        "id": "sg:person.01125651721.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125651721.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Integrated DNA Technologies (United States)", 
          "id": "https://www.grid.ac/institutes/grid.420360.3", 
          "name": [
            "Integrated DNA Technologies, Inc., 1710 Commercial Park, 5224, Coralville, IA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Beltz", 
        "givenName": "Kristin R", 
        "id": "sg:person.01007552234.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007552234.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Integrated DNA Technologies (United States)", 
          "id": "https://www.grid.ac/institutes/grid.420360.3", 
          "name": [
            "Integrated DNA Technologies, Inc., 1710 Commercial Park, 5224, Coralville, IA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rupp", 
        "givenName": "Susan M", 
        "id": "sg:person.01055665434.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055665434.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Integrated DNA Technologies (United States)", 
          "id": "https://www.grid.ac/institutes/grid.420360.3", 
          "name": [
            "Integrated DNA Technologies, Inc., 1710 Commercial Park, 5224, Coralville, IA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Powers", 
        "givenName": "Kristy M", 
        "id": "sg:person.01124000634.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124000634.82"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Integrated DNA Technologies (United States)", 
          "id": "https://www.grid.ac/institutes/grid.420360.3", 
          "name": [
            "Integrated DNA Technologies, Inc., 1710 Commercial Park, 5224, Coralville, IA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Behlke", 
        "givenName": "Mark A", 
        "id": "sg:person.01232754567.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232754567.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Integrated DNA Technologies (United States)", 
          "id": "https://www.grid.ac/institutes/grid.420360.3", 
          "name": [
            "Integrated DNA Technologies, Inc., 1710 Commercial Park, 5224, Coralville, IA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Walder", 
        "givenName": "Joseph A", 
        "id": "sg:person.0637512046.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637512046.37"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0888-7543(89)90280-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004053545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2007.03.075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004853840"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0396-303", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007118508", 
          "https://doi.org/10.1038/nbt0396-303"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1373/clinchem.2008.112797", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007780642"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2003.08.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009480193"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2003.08.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009480193"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bioeng.9.060906.152037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010293216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.sbi.2007.12.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016707512"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcla.20240", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016798148"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/17.7.2503", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018135284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/344768a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021070239", 
          "https://doi.org/10.1038/344768a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2007.09.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022344505"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.5.2244", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023359823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.36.22591", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024487184"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/381445a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024656548", 
          "https://doi.org/10.1038/381445a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/13.13.4811", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024726518"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0300-9084(93)90033-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024909578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0300-9084(93)90033-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024909578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cccn.2005.04.037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025303538"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cccn.2005.04.037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025303538"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.genom.1.1.329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027712632"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.16.7276", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029593996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/26.14.3392", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029848162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/mcpr.1993.1036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031238323"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/20.7.1717", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033089401"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/30.2.598", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033427118"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/geno.1994.1159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034105035"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2003.03381.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037008003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.22.8923", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039123289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2009.10.042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040551214"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1050-3862(98)00019-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041654045"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.262591699", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042894994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2007.06.056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047147415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1746-4811-3-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047777871", 
          "https://doi.org/10.1186/1746-4811-3-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.00268-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048194885"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mcp.2003.08.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048930139"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mcp.2003.08.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048930139"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.12.4580", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049768055"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/humu.10228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050737756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-1759(02)00218-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050839435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.2.1.14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051474568"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/28.2.655", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051874341"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2003.09.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052234050"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi026960a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055196489"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi026960a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055196489"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/oli.2007.0090", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059303354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jbchem.a022514", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059961315"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.3413476", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062613076"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/000112960", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069095906"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/00295rr03", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074730445"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077283380", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077315045", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082639551", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082801381", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/98241pf01", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083207552"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083352595", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-12", 
    "datePublishedReg": "2011-12-01", 
    "description": "BACKGROUND: The polymerase chain reaction (PCR) is commonly used to detect the presence of nucleic acid sequences both in research and diagnostic settings. While high specificity is often achieved, biological requirements sometimes necessitate that primers are placed in suboptimal locations which lead to problems with the formation of primer dimers and/or misamplification of homologous sequences.\nRESULTS: Pyrococcus abyssi (P.a.) RNase H2 was used to enable PCR to be performed using blocked primers containing a single ribonucleotide residue which are activated via cleavage by the enzyme (rhPCR). Cleavage occurs 5'-to the RNA base following primer hybridization to the target DNA. The requirement of the primer to first hybridize with the target sequence to gain activity eliminates the formation of primer-dimers and greatly reduces misamplification of closely related sequences. Mismatches near the scissile linkage decrease the efficiency of cleavage by RNase H2, further increasing the specificity of the assay. When applied to the detection of single nucleotide polymorphisms (SNPs), rhPCR was found to be far more sensitive than standard allele-specific PCR. In general, the best discrimination occurs when the mismatch is placed at the RNA:DNA base pair.\nCONCLUSION: rhPCR eliminates the formation of primer dimers and markedly improves the specificity of PCR with respect to off-target amplification. These advantages of the assay should find utility in challenging qPCR applications such as genotyping, high level multiplex assays and rare allele detection.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1472-6750-11-80", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1028420", 
        "issn": [
          "1472-6750"
        ], 
        "name": "BMC Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "11"
      }
    ], 
    "name": "RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers", 
    "pagination": "80", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "78e2bd558214f3b307bb382e8f05b678b0cb686fdc3c927cc62c9dfded2e0dd4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21831278"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101088663"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1472-6750-11-80"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1025374506"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1472-6750-11-80", 
      "https://app.dimensions.ai/details/publication/pub.1025374506"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T02:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000512.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1472-6750-11-80"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1472-6750-11-80'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1472-6750-11-80'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1472-6750-11-80'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1472-6750-11-80'


 

This table displays all metadata directly associated to this object as RDF triples.

310 TRIPLES      21 PREDICATES      92 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1472-6750-11-80 schema:about N0e67d4698cb44c1db74773bf6c19bcfe
2 N12828ce9d3c14813aa9916f438bf8a7a
3 N1ca955ac85174a5fbd77c528010fcb25
4 N3630c34fbba54794b06caf368121bff8
5 N6163301742f144a1ba8eeada1e95e066
6 N68809da4b7834c8bb3d734af0c7bc17c
7 N72de5fcbade347adb404acb94ccf369f
8 N98d8063a735545a4903b627d9b6eba1d
9 Na17c2c4ee7694f02803fe37106881d84
10 Na48827dc323045c4addff82fd280b8fb
11 Nbc0a8c1ac87b49608ef385c819812aa5
12 Ne19c0abe1d364bcba987197b32ddd1ac
13 anzsrc-for:06
14 anzsrc-for:0604
15 schema:author Nf857a54ff1714daf83446a984c9c2d47
16 schema:citation sg:pub.10.1038/344768a0
17 sg:pub.10.1038/381445a0
18 sg:pub.10.1038/nbt0396-303
19 sg:pub.10.1186/1746-4811-3-2
20 https://app.dimensions.ai/details/publication/pub.1077283380
21 https://app.dimensions.ai/details/publication/pub.1077315045
22 https://app.dimensions.ai/details/publication/pub.1082639551
23 https://app.dimensions.ai/details/publication/pub.1082801381
24 https://app.dimensions.ai/details/publication/pub.1083352595
25 https://doi.org/10.1002/humu.10228
26 https://doi.org/10.1002/jcla.20240
27 https://doi.org/10.1006/geno.1994.1159
28 https://doi.org/10.1006/mcpr.1993.1036
29 https://doi.org/10.1016/0300-9084(93)90033-o
30 https://doi.org/10.1016/0888-7543(89)90280-2
31 https://doi.org/10.1016/j.ab.2003.09.003
32 https://doi.org/10.1016/j.ab.2007.09.002
33 https://doi.org/10.1016/j.ab.2009.10.042
34 https://doi.org/10.1016/j.bbrc.2003.08.003
35 https://doi.org/10.1016/j.bbrc.2007.03.075
36 https://doi.org/10.1016/j.cccn.2005.04.037
37 https://doi.org/10.1016/j.jmb.2007.06.056
38 https://doi.org/10.1016/j.mcp.2003.08.004
39 https://doi.org/10.1016/j.sbi.2007.12.008
40 https://doi.org/10.1016/s0022-1759(02)00218-1
41 https://doi.org/10.1016/s1050-3862(98)00019-9
42 https://doi.org/10.1021/bi026960a
43 https://doi.org/10.1046/j.1365-2958.2003.03381.x
44 https://doi.org/10.1073/pnas.262591699
45 https://doi.org/10.1073/pnas.87.12.4580
46 https://doi.org/10.1073/pnas.87.22.8923
47 https://doi.org/10.1073/pnas.88.16.7276
48 https://doi.org/10.1073/pnas.95.5.2244
49 https://doi.org/10.1074/jbc.272.36.22591
50 https://doi.org/10.1089/oli.2007.0090
51 https://doi.org/10.1093/nar/13.13.4811
52 https://doi.org/10.1093/nar/17.7.2503
53 https://doi.org/10.1093/nar/20.7.1717
54 https://doi.org/10.1093/nar/26.14.3392
55 https://doi.org/10.1093/nar/28.2.655
56 https://doi.org/10.1093/nar/30.2.598
57 https://doi.org/10.1093/oxfordjournals.jbchem.a022514
58 https://doi.org/10.1101/gr.2.1.14
59 https://doi.org/10.1126/science.3413476
60 https://doi.org/10.1128/jb.00268-10
61 https://doi.org/10.1146/annurev.bioeng.9.060906.152037
62 https://doi.org/10.1146/annurev.genom.1.1.329
63 https://doi.org/10.1373/clinchem.2008.112797
64 https://doi.org/10.2144/000112960
65 https://doi.org/10.2144/00295rr03
66 https://doi.org/10.2144/98241pf01
67 schema:datePublished 2011-12
68 schema:datePublishedReg 2011-12-01
69 schema:description BACKGROUND: The polymerase chain reaction (PCR) is commonly used to detect the presence of nucleic acid sequences both in research and diagnostic settings. While high specificity is often achieved, biological requirements sometimes necessitate that primers are placed in suboptimal locations which lead to problems with the formation of primer dimers and/or misamplification of homologous sequences. RESULTS: Pyrococcus abyssi (P.a.) RNase H2 was used to enable PCR to be performed using blocked primers containing a single ribonucleotide residue which are activated via cleavage by the enzyme (rhPCR). Cleavage occurs 5'-to the RNA base following primer hybridization to the target DNA. The requirement of the primer to first hybridize with the target sequence to gain activity eliminates the formation of primer-dimers and greatly reduces misamplification of closely related sequences. Mismatches near the scissile linkage decrease the efficiency of cleavage by RNase H2, further increasing the specificity of the assay. When applied to the detection of single nucleotide polymorphisms (SNPs), rhPCR was found to be far more sensitive than standard allele-specific PCR. In general, the best discrimination occurs when the mismatch is placed at the RNA:DNA base pair. CONCLUSION: rhPCR eliminates the formation of primer dimers and markedly improves the specificity of PCR with respect to off-target amplification. These advantages of the assay should find utility in challenging qPCR applications such as genotyping, high level multiplex assays and rare allele detection.
70 schema:genre research_article
71 schema:inLanguage en
72 schema:isAccessibleForFree true
73 schema:isPartOf Nac6593a4725c44cda0cbcf53d93fd317
74 Nb46abe109c814c4a9b54d3b9c045c9b0
75 sg:journal.1028420
76 schema:name RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers
77 schema:pagination 80
78 schema:productId N03d3af5671e845dda2d1db086f8fac99
79 N25ddffe7c4634a328f24f09710ca8050
80 N3f86f57791544947bc2c8b8dc735fb68
81 Nb234e10243534cd5b70b55247e347876
82 Nd39b4e951f2c4e1d890952362c2f7868
83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025374506
84 https://doi.org/10.1186/1472-6750-11-80
85 schema:sdDatePublished 2019-04-11T02:01
86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
87 schema:sdPublisher Nb818cfef2a2c4c51bef26a5e9bdc955d
88 schema:url http://link.springer.com/10.1186%2F1472-6750-11-80
89 sgo:license sg:explorer/license/
90 sgo:sdDataset articles
91 rdf:type schema:ScholarlyArticle
92 N03d3af5671e845dda2d1db086f8fac99 schema:name pubmed_id
93 schema:value 21831278
94 rdf:type schema:PropertyValue
95 N0e67d4698cb44c1db74773bf6c19bcfe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Humans
97 rdf:type schema:DefinedTerm
98 N12828ce9d3c14813aa9916f438bf8a7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Pyrococcus abyssi
100 rdf:type schema:DefinedTerm
101 N1ca955ac85174a5fbd77c528010fcb25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name DNA Primers
103 rdf:type schema:DefinedTerm
104 N20f507c348ae4b488f9d17e5d5deb687 rdf:first sg:person.0637512046.37
105 rdf:rest rdf:nil
106 N25ddffe7c4634a328f24f09710ca8050 schema:name dimensions_id
107 schema:value pub.1025374506
108 rdf:type schema:PropertyValue
109 N3630c34fbba54794b06caf368121bff8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Polymerase Chain Reaction
111 rdf:type schema:DefinedTerm
112 N36b2591b8a94490fadf8792219335044 rdf:first sg:person.01007552234.70
113 rdf:rest Nfb8a6b1bdca24d5d90594b930c113562
114 N3f86f57791544947bc2c8b8dc735fb68 schema:name readcube_id
115 schema:value 78e2bd558214f3b307bb382e8f05b678b0cb686fdc3c927cc62c9dfded2e0dd4
116 rdf:type schema:PropertyValue
117 N6163301742f144a1ba8eeada1e95e066 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Molecular Sequence Data
119 rdf:type schema:DefinedTerm
120 N68809da4b7834c8bb3d734af0c7bc17c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Temperature
122 rdf:type schema:DefinedTerm
123 N72de5fcbade347adb404acb94ccf369f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Nucleic Acid Hybridization
125 rdf:type schema:DefinedTerm
126 N98d8063a735545a4903b627d9b6eba1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Base Sequence
128 rdf:type schema:DefinedTerm
129 Na17c2c4ee7694f02803fe37106881d84 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Sensitivity and Specificity
131 rdf:type schema:DefinedTerm
132 Na48827dc323045c4addff82fd280b8fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Enzyme Stability
134 rdf:type schema:DefinedTerm
135 Nac6593a4725c44cda0cbcf53d93fd317 schema:volumeNumber 11
136 rdf:type schema:PublicationVolume
137 Nb234e10243534cd5b70b55247e347876 schema:name nlm_unique_id
138 schema:value 101088663
139 rdf:type schema:PropertyValue
140 Nb46abe109c814c4a9b54d3b9c045c9b0 schema:issueNumber 1
141 rdf:type schema:PublicationIssue
142 Nb818cfef2a2c4c51bef26a5e9bdc955d schema:name Springer Nature - SN SciGraph project
143 rdf:type schema:Organization
144 Nbc0a8c1ac87b49608ef385c819812aa5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Ribonuclease H
146 rdf:type schema:DefinedTerm
147 Nbf200a7f6de04c1ca896c896f243636f rdf:first sg:person.01125651721.21
148 rdf:rest N36b2591b8a94490fadf8792219335044
149 Nd39b4e951f2c4e1d890952362c2f7868 schema:name doi
150 schema:value 10.1186/1472-6750-11-80
151 rdf:type schema:PropertyValue
152 Nda096d18b65b4498a56011463a0c8df4 rdf:first sg:person.01124000634.82
153 rdf:rest Nef68bae2c2924b75b6a7297751b157b6
154 Ne19c0abe1d364bcba987197b32ddd1ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Polymorphism, Single Nucleotide
156 rdf:type schema:DefinedTerm
157 Nef68bae2c2924b75b6a7297751b157b6 rdf:first sg:person.01232754567.66
158 rdf:rest N20f507c348ae4b488f9d17e5d5deb687
159 Nf857a54ff1714daf83446a984c9c2d47 rdf:first sg:person.011502316477.64
160 rdf:rest Nbf200a7f6de04c1ca896c896f243636f
161 Nfb8a6b1bdca24d5d90594b930c113562 rdf:first sg:person.01055665434.07
162 rdf:rest Nda096d18b65b4498a56011463a0c8df4
163 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
164 schema:name Biological Sciences
165 rdf:type schema:DefinedTerm
166 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
167 schema:name Genetics
168 rdf:type schema:DefinedTerm
169 sg:journal.1028420 schema:issn 1472-6750
170 schema:name BMC Biotechnology
171 rdf:type schema:Periodical
172 sg:person.01007552234.70 schema:affiliation https://www.grid.ac/institutes/grid.420360.3
173 schema:familyName Beltz
174 schema:givenName Kristin R
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007552234.70
176 rdf:type schema:Person
177 sg:person.01055665434.07 schema:affiliation https://www.grid.ac/institutes/grid.420360.3
178 schema:familyName Rupp
179 schema:givenName Susan M
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055665434.07
181 rdf:type schema:Person
182 sg:person.01124000634.82 schema:affiliation https://www.grid.ac/institutes/grid.420360.3
183 schema:familyName Powers
184 schema:givenName Kristy M
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124000634.82
186 rdf:type schema:Person
187 sg:person.01125651721.21 schema:affiliation https://www.grid.ac/institutes/grid.420360.3
188 schema:familyName Rose
189 schema:givenName Scott D
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125651721.21
191 rdf:type schema:Person
192 sg:person.011502316477.64 schema:affiliation https://www.grid.ac/institutes/grid.420360.3
193 schema:familyName Dobosy
194 schema:givenName Joseph R
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011502316477.64
196 rdf:type schema:Person
197 sg:person.01232754567.66 schema:affiliation https://www.grid.ac/institutes/grid.420360.3
198 schema:familyName Behlke
199 schema:givenName Mark A
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232754567.66
201 rdf:type schema:Person
202 sg:person.0637512046.37 schema:affiliation https://www.grid.ac/institutes/grid.420360.3
203 schema:familyName Walder
204 schema:givenName Joseph A
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637512046.37
206 rdf:type schema:Person
207 sg:pub.10.1038/344768a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021070239
208 https://doi.org/10.1038/344768a0
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/381445a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024656548
211 https://doi.org/10.1038/381445a0
212 rdf:type schema:CreativeWork
213 sg:pub.10.1038/nbt0396-303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007118508
214 https://doi.org/10.1038/nbt0396-303
215 rdf:type schema:CreativeWork
216 sg:pub.10.1186/1746-4811-3-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047777871
217 https://doi.org/10.1186/1746-4811-3-2
218 rdf:type schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1077283380 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1077315045 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1082639551 schema:CreativeWork
222 https://app.dimensions.ai/details/publication/pub.1082801381 schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1083352595 schema:CreativeWork
224 https://doi.org/10.1002/humu.10228 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050737756
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1002/jcla.20240 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016798148
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1006/geno.1994.1159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034105035
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1006/mcpr.1993.1036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031238323
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/0300-9084(93)90033-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1024909578
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/0888-7543(89)90280-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004053545
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/j.ab.2003.09.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052234050
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/j.ab.2007.09.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022344505
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/j.ab.2009.10.042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040551214
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/j.bbrc.2003.08.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009480193
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/j.bbrc.2007.03.075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004853840
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/j.cccn.2005.04.037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025303538
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/j.jmb.2007.06.056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047147415
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/j.mcp.2003.08.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048930139
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/j.sbi.2007.12.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016707512
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/s0022-1759(02)00218-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050839435
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/s1050-3862(98)00019-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041654045
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1021/bi026960a schema:sameAs https://app.dimensions.ai/details/publication/pub.1055196489
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1046/j.1365-2958.2003.03381.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037008003
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1073/pnas.262591699 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042894994
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1073/pnas.87.12.4580 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049768055
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1073/pnas.87.22.8923 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039123289
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1073/pnas.88.16.7276 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029593996
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1073/pnas.95.5.2244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023359823
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1074/jbc.272.36.22591 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024487184
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1089/oli.2007.0090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059303354
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1093/nar/13.13.4811 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024726518
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1093/nar/17.7.2503 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018135284
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1093/nar/20.7.1717 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033089401
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1093/nar/26.14.3392 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029848162
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1093/nar/28.2.655 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051874341
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1093/nar/30.2.598 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033427118
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1093/oxfordjournals.jbchem.a022514 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059961315
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1101/gr.2.1.14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051474568
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1126/science.3413476 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062613076
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1128/jb.00268-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048194885
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1146/annurev.bioeng.9.060906.152037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010293216
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1146/annurev.genom.1.1.329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027712632
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1373/clinchem.2008.112797 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007780642
301 rdf:type schema:CreativeWork
302 https://doi.org/10.2144/000112960 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069095906
303 rdf:type schema:CreativeWork
304 https://doi.org/10.2144/00295rr03 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074730445
305 rdf:type schema:CreativeWork
306 https://doi.org/10.2144/98241pf01 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083207552
307 rdf:type schema:CreativeWork
308 https://www.grid.ac/institutes/grid.420360.3 schema:alternateName Integrated DNA Technologies (United States)
309 schema:name Integrated DNA Technologies, Inc., 1710 Commercial Park, 5224, Coralville, IA, USA
310 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...