Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-12

AUTHORS

Zhen Wang, Shafei Ye, Jingjing Li, Bo Zheng, Manzhu Bao, Guogui Ning

ABSTRACT

BACKGROUND: The advent of genomics-based technologies has revolutionized many fields of biological enquiry. However, chromosome walking or flanking sequence cloning is still a necessary and important procedure to determining gene structure. Such methods are used to identify T-DNA insertion sites and so are especially relevant for organisms where large T-DNA insertion libraries have been created, such as rice and Arabidopsis. The currently available methods for flanking sequence cloning, including the popular TAIL-PCR technique, are relatively laborious and slow. RESULTS: Here, we report a simple and effective fusion primer and nested integrated PCR method (FPNI-PCR) for the identification and cloning of unknown genomic regions flanked known sequences. In brief, a set of universal primers was designed that consisted of various 15-16 base arbitrary degenerate oligonucleotides. These arbitrary degenerate primers were fused to the 3' end of an adaptor oligonucleotide which provided a known sequence without degenerate nucleotides, thereby forming the fusion primers (FPs). These fusion primers are employed in the first step of an integrated nested PCR strategy which defines the overall FPNI-PCR protocol. In order to demonstrate the efficacy of this novel strategy, we have successfully used it to isolate multiple genomic sequences namely, 21 orthologs of genes in various species of Rosaceace, 4 MYB genes of Rosa rugosa, 3 promoters of transcription factors of Petunia hybrida, and 4 flanking sequences of T-DNA insertion sites in transgenic tobacco lines and 6 specific genes from sequenced genome of rice and Arabidopsis. CONCLUSIONS: The successful amplification of target products through FPNI-PCR verified that this novel strategy is an effective, low cost and simple procedure. Furthermore, FPNI-PCR represents a more sensitive, rapid and accurate technique than the established TAIL-PCR and hiTAIL-PCR procedures. More... »

PAGES

109

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1472-6750-11-109

DOI

http://dx.doi.org/10.1186/1472-6750-11-109

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021988506

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22093809


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosome Walking", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Primers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Intergenic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oligonucleotides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plants", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Huazhong Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.35155.37", 
          "name": [
            "Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Zhen", 
        "id": "sg:person.0741603253.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741603253.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Huazhong Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.35155.37", 
          "name": [
            "Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ye", 
        "givenName": "Shafei", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Huazhong Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.35155.37", 
          "name": [
            "Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Jingjing", 
        "id": "sg:person.0657377546.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657377546.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Huazhong Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.35155.37", 
          "name": [
            "Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zheng", 
        "givenName": "Bo", 
        "id": "sg:person.0606671360.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606671360.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Huazhong Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.35155.37", 
          "name": [
            "Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bao", 
        "givenName": "Manzhu", 
        "id": "sg:person.014372264272.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014372264272.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Huazhong Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.35155.37", 
          "name": [
            "Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ning", 
        "givenName": "Guogui", 
        "id": "sg:person.01043506072.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043506072.87"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1128/aem.02973-06", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002707894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-010-2613-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003222092", 
          "https://doi.org/10.1007/s00253-010-2613-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-010-2613-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003222092", 
          "https://doi.org/10.1007/s00253-010-2613-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1995.08030457.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013034175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gni124", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018556832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gng068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019279057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-009-2211-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022725431", 
          "https://doi.org/10.1007/s00253-009-2211-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-009-2211-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022725431", 
          "https://doi.org/10.1007/s00253-009-2211-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-009-2211-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022725431", 
          "https://doi.org/10.1007/s00253-009-2211-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11262-008-0266-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024665436", 
          "https://doi.org/10.1007/s11262-008-0266-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0888-7543(95)80010-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027027849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0888-7543(95)80010-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027027849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1439-0523.2008.01536.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027246865"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10142-005-0138-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029383951", 
          "https://doi.org/10.1007/s10142-005-0138-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10142-005-0138-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029383951", 
          "https://doi.org/10.1007/s10142-005-0138-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/23.6.1087", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032701642"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02772651", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035044939", 
          "https://doi.org/10.1007/bf02772651"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0010577", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035084683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1012211011538", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036106372", 
          "https://doi.org/10.1023/a:1012211011538"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2008.07.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038075437"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/dnares/dsm023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042982787"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12033-010-9332-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049442656", 
          "https://doi.org/10.1007/s12033-010-9332-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/mb:32:2:111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050402410", 
          "https://doi.org/10.1385/mb:32:2:111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/000112210", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069095332"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/000112601", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069095654"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-12", 
    "datePublishedReg": "2011-12-01", 
    "description": "BACKGROUND: The advent of genomics-based technologies has revolutionized many fields of biological enquiry. However, chromosome walking or flanking sequence cloning is still a necessary and important procedure to determining gene structure. Such methods are used to identify T-DNA insertion sites and so are especially relevant for organisms where large T-DNA insertion libraries have been created, such as rice and Arabidopsis. The currently available methods for flanking sequence cloning, including the popular TAIL-PCR technique, are relatively laborious and slow.\nRESULTS: Here, we report a simple and effective fusion primer and nested integrated PCR method (FPNI-PCR) for the identification and cloning of unknown genomic regions flanked known sequences. In brief, a set of universal primers was designed that consisted of various 15-16 base arbitrary degenerate oligonucleotides. These arbitrary degenerate primers were fused to the 3' end of an adaptor oligonucleotide which provided a known sequence without degenerate nucleotides, thereby forming the fusion primers (FPs). These fusion primers are employed in the first step of an integrated nested PCR strategy which defines the overall FPNI-PCR protocol. In order to demonstrate the efficacy of this novel strategy, we have successfully used it to isolate multiple genomic sequences namely, 21 orthologs of genes in various species of Rosaceace, 4 MYB genes of Rosa rugosa, 3 promoters of transcription factors of Petunia hybrida, and 4 flanking sequences of T-DNA insertion sites in transgenic tobacco lines and 6 specific genes from sequenced genome of rice and Arabidopsis.\nCONCLUSIONS: The successful amplification of target products through FPNI-PCR verified that this novel strategy is an effective, low cost and simple procedure. Furthermore, FPNI-PCR represents a more sensitive, rapid and accurate technique than the established TAIL-PCR and hiTAIL-PCR procedures.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1472-6750-11-109", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.4982137", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1028420", 
        "issn": [
          "1472-6750"
        ], 
        "name": "BMC Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "11"
      }
    ], 
    "name": "Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning", 
    "pagination": "109", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "68c201a15a3923f2015c7e8166abd52181ed5486447a3d01383134e5960720ab"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22093809"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101088663"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1472-6750-11-109"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1021988506"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1472-6750-11-109", 
      "https://app.dimensions.ai/details/publication/pub.1021988506"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:20", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000512.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1472-6750-11-109"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1472-6750-11-109'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1472-6750-11-109'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1472-6750-11-109'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1472-6750-11-109'


 

This table displays all metadata directly associated to this object as RDF triples.

208 TRIPLES      21 PREDICATES      58 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1472-6750-11-109 schema:about N00b040fc779b46b0aaa767441253e093
2 N00f1718a5d7d40719a35dba4fbcc84bc
3 N0be1af60a21c42aea2b3aecb4ec11803
4 N4331a54127684a2ea7ad2237e8b09e88
5 N5ced0f91827b481aa3cfb28bc968dfaf
6 N6f2ae1956c814236be68b4ee3bd24873
7 N8a90ecb248324532b20435e8f6cdf7ed
8 N96b37a36b392474abe9d07d8834020a5
9 Ncb9b3cfc958f410280937c9d53e91d31
10 anzsrc-for:06
11 anzsrc-for:0604
12 schema:author N3b886349683f4762a559afa61e741076
13 schema:citation sg:pub.10.1007/bf02772651
14 sg:pub.10.1007/s00253-009-2211-5
15 sg:pub.10.1007/s00253-010-2613-4
16 sg:pub.10.1007/s10142-005-0138-1
17 sg:pub.10.1007/s11262-008-0266-5
18 sg:pub.10.1007/s12033-010-9332-z
19 sg:pub.10.1023/a:1012211011538
20 sg:pub.10.1385/mb:32:2:111
21 https://doi.org/10.1016/0888-7543(95)80010-j
22 https://doi.org/10.1016/j.ab.2008.07.012
23 https://doi.org/10.1046/j.1365-313x.1995.08030457.x
24 https://doi.org/10.1093/dnares/dsm023
25 https://doi.org/10.1093/nar/23.6.1087
26 https://doi.org/10.1093/nar/gng068
27 https://doi.org/10.1093/nar/gni124
28 https://doi.org/10.1111/j.1439-0523.2008.01536.x
29 https://doi.org/10.1128/aem.02973-06
30 https://doi.org/10.1371/journal.pone.0010577
31 https://doi.org/10.2144/000112210
32 https://doi.org/10.2144/000112601
33 schema:datePublished 2011-12
34 schema:datePublishedReg 2011-12-01
35 schema:description BACKGROUND: The advent of genomics-based technologies has revolutionized many fields of biological enquiry. However, chromosome walking or flanking sequence cloning is still a necessary and important procedure to determining gene structure. Such methods are used to identify T-DNA insertion sites and so are especially relevant for organisms where large T-DNA insertion libraries have been created, such as rice and Arabidopsis. The currently available methods for flanking sequence cloning, including the popular TAIL-PCR technique, are relatively laborious and slow. RESULTS: Here, we report a simple and effective fusion primer and nested integrated PCR method (FPNI-PCR) for the identification and cloning of unknown genomic regions flanked known sequences. In brief, a set of universal primers was designed that consisted of various 15-16 base arbitrary degenerate oligonucleotides. These arbitrary degenerate primers were fused to the 3' end of an adaptor oligonucleotide which provided a known sequence without degenerate nucleotides, thereby forming the fusion primers (FPs). These fusion primers are employed in the first step of an integrated nested PCR strategy which defines the overall FPNI-PCR protocol. In order to demonstrate the efficacy of this novel strategy, we have successfully used it to isolate multiple genomic sequences namely, 21 orthologs of genes in various species of Rosaceace, 4 MYB genes of Rosa rugosa, 3 promoters of transcription factors of Petunia hybrida, and 4 flanking sequences of T-DNA insertion sites in transgenic tobacco lines and 6 specific genes from sequenced genome of rice and Arabidopsis. CONCLUSIONS: The successful amplification of target products through FPNI-PCR verified that this novel strategy is an effective, low cost and simple procedure. Furthermore, FPNI-PCR represents a more sensitive, rapid and accurate technique than the established TAIL-PCR and hiTAIL-PCR procedures.
36 schema:genre research_article
37 schema:inLanguage en
38 schema:isAccessibleForFree true
39 schema:isPartOf N09ed7df53f4c444fb345a5e3697e6c02
40 N477f6f8e1d494d77b8d3abb5b1380b5d
41 sg:journal.1028420
42 schema:name Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning
43 schema:pagination 109
44 schema:productId N0ffff2abda674a30a9e489159389a637
45 Nae2bf5337da94b5585f6d2f87b0fb24f
46 Ne1a6c6d506004e6db6df459ce83770f0
47 Ne3b7da418cdd44eeb6de64bd1e966902
48 Neaf7f83a4d354ddc9396b82c505bd45d
49 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021988506
50 https://doi.org/10.1186/1472-6750-11-109
51 schema:sdDatePublished 2019-04-10T18:20
52 schema:sdLicense https://scigraph.springernature.com/explorer/license/
53 schema:sdPublisher N584279c003374b83a3abd521b1480e7d
54 schema:url http://link.springer.com/10.1186%2F1472-6750-11-109
55 sgo:license sg:explorer/license/
56 sgo:sdDataset articles
57 rdf:type schema:ScholarlyArticle
58 N00b040fc779b46b0aaa767441253e093 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
59 schema:name DNA, Bacterial
60 rdf:type schema:DefinedTerm
61 N00f1718a5d7d40719a35dba4fbcc84bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Plants
63 rdf:type schema:DefinedTerm
64 N09ed7df53f4c444fb345a5e3697e6c02 schema:issueNumber 1
65 rdf:type schema:PublicationIssue
66 N0be1af60a21c42aea2b3aecb4ec11803 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
67 schema:name DNA, Intergenic
68 rdf:type schema:DefinedTerm
69 N0dd39d9269b54e4abbb34811410ab710 rdf:first sg:person.014372264272.62
70 rdf:rest N5e2b75ff23ca44e2a74d3a9bd0e266c4
71 N0ffff2abda674a30a9e489159389a637 schema:name doi
72 schema:value 10.1186/1472-6750-11-109
73 rdf:type schema:PropertyValue
74 N3b886349683f4762a559afa61e741076 rdf:first sg:person.0741603253.10
75 rdf:rest N60e41d0d1b28475887cdbcb4abf96a4b
76 N3b8f67d343af4d11be43af2f54bc0faa rdf:first sg:person.0606671360.05
77 rdf:rest N0dd39d9269b54e4abbb34811410ab710
78 N4331a54127684a2ea7ad2237e8b09e88 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name DNA Primers
80 rdf:type schema:DefinedTerm
81 N477f6f8e1d494d77b8d3abb5b1380b5d schema:volumeNumber 11
82 rdf:type schema:PublicationVolume
83 N584279c003374b83a3abd521b1480e7d schema:name Springer Nature - SN SciGraph project
84 rdf:type schema:Organization
85 N5ced0f91827b481aa3cfb28bc968dfaf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Oligonucleotides
87 rdf:type schema:DefinedTerm
88 N5e2b75ff23ca44e2a74d3a9bd0e266c4 rdf:first sg:person.01043506072.87
89 rdf:rest rdf:nil
90 N60e41d0d1b28475887cdbcb4abf96a4b rdf:first N9be0a9661ad2426690b367c88ea339e9
91 rdf:rest Ne0dcdb326f304e4bb64e40c5231e82b2
92 N6f2ae1956c814236be68b4ee3bd24873 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Chromosome Walking
94 rdf:type schema:DefinedTerm
95 N8a90ecb248324532b20435e8f6cdf7ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Polymerase Chain Reaction
97 rdf:type schema:DefinedTerm
98 N96b37a36b392474abe9d07d8834020a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Cloning, Molecular
100 rdf:type schema:DefinedTerm
101 N9be0a9661ad2426690b367c88ea339e9 schema:affiliation https://www.grid.ac/institutes/grid.35155.37
102 schema:familyName Ye
103 schema:givenName Shafei
104 rdf:type schema:Person
105 Nae2bf5337da94b5585f6d2f87b0fb24f schema:name readcube_id
106 schema:value 68c201a15a3923f2015c7e8166abd52181ed5486447a3d01383134e5960720ab
107 rdf:type schema:PropertyValue
108 Ncb9b3cfc958f410280937c9d53e91d31 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Binding Sites
110 rdf:type schema:DefinedTerm
111 Ne0dcdb326f304e4bb64e40c5231e82b2 rdf:first sg:person.0657377546.83
112 rdf:rest N3b8f67d343af4d11be43af2f54bc0faa
113 Ne1a6c6d506004e6db6df459ce83770f0 schema:name pubmed_id
114 schema:value 22093809
115 rdf:type schema:PropertyValue
116 Ne3b7da418cdd44eeb6de64bd1e966902 schema:name dimensions_id
117 schema:value pub.1021988506
118 rdf:type schema:PropertyValue
119 Neaf7f83a4d354ddc9396b82c505bd45d schema:name nlm_unique_id
120 schema:value 101088663
121 rdf:type schema:PropertyValue
122 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
123 schema:name Biological Sciences
124 rdf:type schema:DefinedTerm
125 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
126 schema:name Genetics
127 rdf:type schema:DefinedTerm
128 sg:grant.4982137 http://pending.schema.org/fundedItem sg:pub.10.1186/1472-6750-11-109
129 rdf:type schema:MonetaryGrant
130 sg:journal.1028420 schema:issn 1472-6750
131 schema:name BMC Biotechnology
132 rdf:type schema:Periodical
133 sg:person.01043506072.87 schema:affiliation https://www.grid.ac/institutes/grid.35155.37
134 schema:familyName Ning
135 schema:givenName Guogui
136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043506072.87
137 rdf:type schema:Person
138 sg:person.014372264272.62 schema:affiliation https://www.grid.ac/institutes/grid.35155.37
139 schema:familyName Bao
140 schema:givenName Manzhu
141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014372264272.62
142 rdf:type schema:Person
143 sg:person.0606671360.05 schema:affiliation https://www.grid.ac/institutes/grid.35155.37
144 schema:familyName Zheng
145 schema:givenName Bo
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606671360.05
147 rdf:type schema:Person
148 sg:person.0657377546.83 schema:affiliation https://www.grid.ac/institutes/grid.35155.37
149 schema:familyName Li
150 schema:givenName Jingjing
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657377546.83
152 rdf:type schema:Person
153 sg:person.0741603253.10 schema:affiliation https://www.grid.ac/institutes/grid.35155.37
154 schema:familyName Wang
155 schema:givenName Zhen
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741603253.10
157 rdf:type schema:Person
158 sg:pub.10.1007/bf02772651 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035044939
159 https://doi.org/10.1007/bf02772651
160 rdf:type schema:CreativeWork
161 sg:pub.10.1007/s00253-009-2211-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022725431
162 https://doi.org/10.1007/s00253-009-2211-5
163 rdf:type schema:CreativeWork
164 sg:pub.10.1007/s00253-010-2613-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003222092
165 https://doi.org/10.1007/s00253-010-2613-4
166 rdf:type schema:CreativeWork
167 sg:pub.10.1007/s10142-005-0138-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029383951
168 https://doi.org/10.1007/s10142-005-0138-1
169 rdf:type schema:CreativeWork
170 sg:pub.10.1007/s11262-008-0266-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024665436
171 https://doi.org/10.1007/s11262-008-0266-5
172 rdf:type schema:CreativeWork
173 sg:pub.10.1007/s12033-010-9332-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1049442656
174 https://doi.org/10.1007/s12033-010-9332-z
175 rdf:type schema:CreativeWork
176 sg:pub.10.1023/a:1012211011538 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036106372
177 https://doi.org/10.1023/a:1012211011538
178 rdf:type schema:CreativeWork
179 sg:pub.10.1385/mb:32:2:111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050402410
180 https://doi.org/10.1385/mb:32:2:111
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/0888-7543(95)80010-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1027027849
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1016/j.ab.2008.07.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038075437
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1046/j.1365-313x.1995.08030457.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013034175
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1093/dnares/dsm023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042982787
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1093/nar/23.6.1087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032701642
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1093/nar/gng068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019279057
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1093/nar/gni124 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018556832
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1111/j.1439-0523.2008.01536.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1027246865
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1128/aem.02973-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002707894
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1371/journal.pone.0010577 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035084683
201 rdf:type schema:CreativeWork
202 https://doi.org/10.2144/000112210 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069095332
203 rdf:type schema:CreativeWork
204 https://doi.org/10.2144/000112601 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069095654
205 rdf:type schema:CreativeWork
206 https://www.grid.ac/institutes/grid.35155.37 schema:alternateName Huazhong Agricultural University
207 schema:name Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, P. R. China
208 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...