Promoter methylation inhibits BRD7 expression in human nasopharyngeal carcinoma cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-09-08

AUTHORS

Huaying Liu, Liming Zhang, Zhaoxia Niu, Ming Zhou, Cong Peng, Xiayu Li, Tan Deng, Lei Shi, Yixin Tan, Guiyuan Li

ABSTRACT

BackgroundNasopharyngeal carcinoma (NPC) is a head and neck malignancy with high occurrence in South-East Asia and Southern China. Recent findings suggest that epigenetic inactivation of multiple tumor suppressor genes plays an important role in the tumourigenesis of NPC. BRD7 is a NPC-associated bromodomain gene that exhibits a much higher-level of mRNA expression in normal than in NPC biopsies and cell lines. In this study, we explored the role of DNA methylation in regulation of BRD7 transcription.MethodsThe presence of CpG islands within BRD7 promoter was predicted by EMBOSS CpGplot and Softberry CpGFinder, respectively. Nested methylation-specific PCR and RT-PCR were employed to detect the methylation status of BRD7 promoter and the mRNA expression of BRD7 gene in tumor cell lines as well as clinical samples. Electrophoretic mobility shift assays (EMSA) and luciferase assay were used to detect the effects of cytosine methylation on the nuclear protein binding to BRD7 promoter.ResultsWe found that DNA methylation suppresses BRD7 expression in NPC cells. In vitro DNA methylation in NPC cells silenced BRD7 promoter activity and inhibited the binding of the nuclear protein (possibly Sp1) to Sp1 binding sites in the BRD7 promoter. In contrast, inhibition of DNA methylation augments induction of endogenous BRD7 mRNA in NPC cells. We also found that methylation frequency of BRD7 promoter is much higher in the tumor and matched blood samples from NPC patients than in the blood samples from normal individuals.ConclusionBRD7 promoter demethylation is a prerequisite for high level induction of BRD7 gene expression. DNA methylation of BRD7 promoter might serve as a diagnostic marker in NPC. More... »

PAGES

253

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2407-8-253

DOI

http://dx.doi.org/10.1186/1471-2407-8-253

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049458832

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18778484


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1117", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Public Health and Health Services", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carcinoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomal Proteins, Non-Histone", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CpG Islands", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytosine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Methylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epigenesis, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HeLa Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nasopharyngeal Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Tertiary", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China", 
          "id": "http://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Huaying", 
        "id": "sg:person.01215235247.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215235247.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China", 
          "id": "http://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Liming", 
        "id": "sg:person.0770421347.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770421347.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China", 
          "id": "http://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Niu", 
        "givenName": "Zhaoxia", 
        "id": "sg:person.01262462443.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01262462443.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China", 
          "id": "http://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Ming", 
        "id": "sg:person.01075456274.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01075456274.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China", 
          "id": "http://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Peng", 
        "givenName": "Cong", 
        "id": "sg:person.0715767315.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715767315.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China", 
          "id": "http://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Xiayu", 
        "id": "sg:person.011030223667.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011030223667.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China", 
          "id": "http://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Deng", 
        "givenName": "Tan", 
        "id": "sg:person.0622113150.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622113150.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China", 
          "id": "http://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shi", 
        "givenName": "Lei", 
        "id": "sg:person.0744166254.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744166254.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China", 
          "id": "http://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tan", 
        "givenName": "Yixin", 
        "id": "sg:person.0605160522.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605160522.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China", 
          "id": "http://www.grid.ac/institutes/grid.216417.7", 
          "name": [
            "Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Guiyuan", 
        "id": "sg:person.0765070774.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765070774.37"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s11010-007-9466-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008996477", 
          "https://doi.org/10.1007/s11010-007-9466-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1209839", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000270224", 
          "https://doi.org/10.1038/sj.onc.1209839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3700547", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019983752", 
          "https://doi.org/10.1038/labinvest.3700547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1207632", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025126353", 
          "https://doi.org/10.1038/sj.onc.1207632"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2008-09-08", 
    "datePublishedReg": "2008-09-08", 
    "description": "BackgroundNasopharyngeal carcinoma (NPC) is a head and neck malignancy with high occurrence in South-East Asia and Southern China. Recent findings suggest that epigenetic inactivation of multiple tumor suppressor genes plays an important role in the tumourigenesis of NPC. BRD7 is a NPC-associated bromodomain gene that exhibits a much higher-level of mRNA expression in normal than in NPC biopsies and cell lines. In this study, we explored the role of DNA methylation in regulation of BRD7 transcription.MethodsThe presence of CpG islands within BRD7 promoter was predicted by EMBOSS CpGplot and Softberry CpGFinder, respectively. Nested methylation-specific PCR and RT-PCR were employed to detect the methylation status of BRD7 promoter and the mRNA expression of BRD7 gene in tumor cell lines as well as clinical samples. Electrophoretic mobility shift assays (EMSA) and luciferase assay were used to detect the effects of cytosine methylation on the nuclear protein binding to BRD7 promoter.ResultsWe found that DNA methylation suppresses BRD7 expression in NPC cells. In vitro DNA methylation in NPC cells silenced BRD7 promoter activity and inhibited the binding of the nuclear protein (possibly Sp1) to Sp1 binding sites in the BRD7 promoter. In contrast, inhibition of DNA methylation augments induction of endogenous BRD7 mRNA in NPC cells. We also found that methylation frequency of BRD7 promoter is much higher in the tumor and matched blood samples from NPC patients than in the blood samples from normal individuals.ConclusionBRD7 promoter demethylation is a prerequisite for high level induction of BRD7 gene expression. DNA methylation of BRD7 promoter might serve as a diagnostic marker in NPC.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/1471-2407-8-253", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.4967602", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4924628", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4988485", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4902429", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1024632", 
        "issn": [
          "1471-2407"
        ], 
        "name": "BMC Cancer", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "keywords": [
      "electrophoretic mobility shift assays", 
      "DNA methylation", 
      "nuclear proteins", 
      "BRD7 expression", 
      "NPC cells", 
      "mobility shift assays", 
      "high-level induction", 
      "cell lines", 
      "tumor suppressor gene", 
      "multiple tumor suppressor genes", 
      "cytosine methylation", 
      "human nasopharyngeal carcinoma cells", 
      "CpG islands", 
      "methylation-specific PCR", 
      "shift assays", 
      "BRD7 gene", 
      "gene expression", 
      "epigenetic inactivation", 
      "promoter activity", 
      "promoter demethylation", 
      "suppressor gene", 
      "methylation", 
      "nasopharyngeal carcinoma cells", 
      "promoter", 
      "methylation status", 
      "tumor cell lines", 
      "mRNA expression", 
      "genes", 
      "methylation frequency", 
      "recent findings", 
      "carcinoma cells", 
      "expression", 
      "protein", 
      "RT-PCR", 
      "cells", 
      "South East Asia", 
      "important role", 
      "Sp1", 
      "induction", 
      "BackgroundNasopharyngeal carcinoma", 
      "transcription", 
      "BRD7", 
      "southern China", 
      "diagnostic marker", 
      "demethylation", 
      "NPC biopsies", 
      "luciferase", 
      "mRNA", 
      "NPC", 
      "regulation", 
      "tumourigenesis", 
      "role", 
      "binding", 
      "inactivation", 
      "lines", 
      "PCR", 
      "higher occurrence", 
      "assays", 
      "islands", 
      "inhibition", 
      "clinical samples", 
      "markers", 
      "sites", 
      "MethodsThe presence", 
      "activity", 
      "normal individuals", 
      "contrast", 
      "prerequisite", 
      "Asia", 
      "presence", 
      "occurrence", 
      "individuals", 
      "NPC patients", 
      "samples", 
      "ResultsWe", 
      "findings", 
      "tumors", 
      "study", 
      "effect", 
      "status", 
      "blood samples", 
      "malignancy", 
      "China", 
      "carcinoma", 
      "head", 
      "frequency", 
      "biopsy", 
      "patients", 
      "neck malignancies"
    ], 
    "name": "Promoter methylation inhibits BRD7 expression in human nasopharyngeal carcinoma cells", 
    "pagination": "253", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1049458832"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2407-8-253"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18778484"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2407-8-253", 
      "https://app.dimensions.ai/details/publication/pub.1049458832"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:34", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_457.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/1471-2407-8-253"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-8-253'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-8-253'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-8-253'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-8-253'


 

This table displays all metadata directly associated to this object as RDF triples.

300 TRIPLES      21 PREDICATES      134 URIs      121 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2407-8-253 schema:about N19ab79cbcd70499dbaaf64c75f598e90
2 N1db156d767454bc99223b060e0c040a8
3 N4f26dc8360a74faf85adf0c21a577500
4 N50a61d54a4b047d8b5ebc10eda5aebbc
5 N5e7605a6ed4d4d928b349252c9163e92
6 N6d5c452329334bc88082df034dfaca65
7 N928402f2f68647c687b69530519d2304
8 N95d50053236e401eb86d250de8bc217f
9 Naadbe578462c4d7f97051c9db4f12adc
10 Nb09feccc619b41aa974063322004617c
11 Nb32f3bcdea2c49d289824915761aaf95
12 Nb33074e2591d44d4836ca75b0f66c4a5
13 Nbf67824211dc444aac8e5f57e8218c15
14 Nc9bc307f69ce4caa8dcf4507c6a6796c
15 Ne6f6dc4858144982a9d0ed103c804b8e
16 anzsrc-for:11
17 anzsrc-for:1112
18 anzsrc-for:1117
19 schema:author N4918331b1c5b476383885fcfeac3c39a
20 schema:citation sg:pub.10.1007/s11010-007-9466-x
21 sg:pub.10.1038/labinvest.3700547
22 sg:pub.10.1038/sj.onc.1207632
23 sg:pub.10.1038/sj.onc.1209839
24 schema:datePublished 2008-09-08
25 schema:datePublishedReg 2008-09-08
26 schema:description BackgroundNasopharyngeal carcinoma (NPC) is a head and neck malignancy with high occurrence in South-East Asia and Southern China. Recent findings suggest that epigenetic inactivation of multiple tumor suppressor genes plays an important role in the tumourigenesis of NPC. BRD7 is a NPC-associated bromodomain gene that exhibits a much higher-level of mRNA expression in normal than in NPC biopsies and cell lines. In this study, we explored the role of DNA methylation in regulation of BRD7 transcription.MethodsThe presence of CpG islands within BRD7 promoter was predicted by EMBOSS CpGplot and Softberry CpGFinder, respectively. Nested methylation-specific PCR and RT-PCR were employed to detect the methylation status of BRD7 promoter and the mRNA expression of BRD7 gene in tumor cell lines as well as clinical samples. Electrophoretic mobility shift assays (EMSA) and luciferase assay were used to detect the effects of cytosine methylation on the nuclear protein binding to BRD7 promoter.ResultsWe found that DNA methylation suppresses BRD7 expression in NPC cells. In vitro DNA methylation in NPC cells silenced BRD7 promoter activity and inhibited the binding of the nuclear protein (possibly Sp1) to Sp1 binding sites in the BRD7 promoter. In contrast, inhibition of DNA methylation augments induction of endogenous BRD7 mRNA in NPC cells. We also found that methylation frequency of BRD7 promoter is much higher in the tumor and matched blood samples from NPC patients than in the blood samples from normal individuals.ConclusionBRD7 promoter demethylation is a prerequisite for high level induction of BRD7 gene expression. DNA methylation of BRD7 promoter might serve as a diagnostic marker in NPC.
27 schema:genre article
28 schema:isAccessibleForFree true
29 schema:isPartOf N7e5a241cf30c4143b42f3e3dce32c7fc
30 Nf1f6958e2dc548b6b9700b2a09ba0b2a
31 sg:journal.1024632
32 schema:keywords Asia
33 BRD7
34 BRD7 expression
35 BRD7 gene
36 BackgroundNasopharyngeal carcinoma
37 China
38 CpG islands
39 DNA methylation
40 MethodsThe presence
41 NPC
42 NPC biopsies
43 NPC cells
44 NPC patients
45 PCR
46 RT-PCR
47 ResultsWe
48 South East Asia
49 Sp1
50 activity
51 assays
52 binding
53 biopsy
54 blood samples
55 carcinoma
56 carcinoma cells
57 cell lines
58 cells
59 clinical samples
60 contrast
61 cytosine methylation
62 demethylation
63 diagnostic marker
64 effect
65 electrophoretic mobility shift assays
66 epigenetic inactivation
67 expression
68 findings
69 frequency
70 gene expression
71 genes
72 head
73 high-level induction
74 higher occurrence
75 human nasopharyngeal carcinoma cells
76 important role
77 inactivation
78 individuals
79 induction
80 inhibition
81 islands
82 lines
83 luciferase
84 mRNA
85 mRNA expression
86 malignancy
87 markers
88 methylation
89 methylation frequency
90 methylation status
91 methylation-specific PCR
92 mobility shift assays
93 multiple tumor suppressor genes
94 nasopharyngeal carcinoma cells
95 neck malignancies
96 normal individuals
97 nuclear proteins
98 occurrence
99 patients
100 prerequisite
101 presence
102 promoter
103 promoter activity
104 promoter demethylation
105 protein
106 recent findings
107 regulation
108 role
109 samples
110 shift assays
111 sites
112 southern China
113 status
114 study
115 suppressor gene
116 transcription
117 tumor cell lines
118 tumor suppressor gene
119 tumors
120 tumourigenesis
121 schema:name Promoter methylation inhibits BRD7 expression in human nasopharyngeal carcinoma cells
122 schema:pagination 253
123 schema:productId N0b8409a8d82347698449d3cc4e100d0c
124 N17ccc15bc1a145f4a2d93421e1ddf0d3
125 Ne6916366d1cb430e8e324c8d1f4eb075
126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049458832
127 https://doi.org/10.1186/1471-2407-8-253
128 schema:sdDatePublished 2022-10-01T06:34
129 schema:sdLicense https://scigraph.springernature.com/explorer/license/
130 schema:sdPublisher N36f7e5dea2c04ace9ca3a0be08409fdc
131 schema:url https://doi.org/10.1186/1471-2407-8-253
132 sgo:license sg:explorer/license/
133 sgo:sdDataset articles
134 rdf:type schema:ScholarlyArticle
135 N0b8409a8d82347698449d3cc4e100d0c schema:name pubmed_id
136 schema:value 18778484
137 rdf:type schema:PropertyValue
138 N17012a6af5214a4da4cf8d7994dd2266 rdf:first sg:person.0605160522.43
139 rdf:rest N71ff0f9bbbb542848122b856c6ea57d2
140 N17ccc15bc1a145f4a2d93421e1ddf0d3 schema:name dimensions_id
141 schema:value pub.1049458832
142 rdf:type schema:PropertyValue
143 N19ab79cbcd70499dbaaf64c75f598e90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Promoter Regions, Genetic
145 rdf:type schema:DefinedTerm
146 N1d5364c2af8e478fac855762d6e508f7 rdf:first sg:person.01075456274.31
147 rdf:rest N60e0ec66ad8442938b3cf2cf955d138d
148 N1db156d767454bc99223b060e0c040a8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Carcinoma
150 rdf:type schema:DefinedTerm
151 N2773871c5aa24c5fb30a3f42ae191637 rdf:first sg:person.0770421347.49
152 rdf:rest Neb57e5576c52479f9842fbfd24573ee9
153 N36f7e5dea2c04ace9ca3a0be08409fdc schema:name Springer Nature - SN SciGraph project
154 rdf:type schema:Organization
155 N47ffb0a5e9fe4eaaafe93b3b8e9d95a1 rdf:first sg:person.011030223667.72
156 rdf:rest Ne20870bca51e48b6b42412fb99afe440
157 N4918331b1c5b476383885fcfeac3c39a rdf:first sg:person.01215235247.05
158 rdf:rest N2773871c5aa24c5fb30a3f42ae191637
159 N4f26dc8360a74faf85adf0c21a577500 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Cell Line, Tumor
161 rdf:type schema:DefinedTerm
162 N50a61d54a4b047d8b5ebc10eda5aebbc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name DNA Methylation
164 rdf:type schema:DefinedTerm
165 N54d9fe67c0c64eb38e50d36cb01085bb rdf:first sg:person.0744166254.21
166 rdf:rest N17012a6af5214a4da4cf8d7994dd2266
167 N5e7605a6ed4d4d928b349252c9163e92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name CpG Islands
169 rdf:type schema:DefinedTerm
170 N60e0ec66ad8442938b3cf2cf955d138d rdf:first sg:person.0715767315.55
171 rdf:rest N47ffb0a5e9fe4eaaafe93b3b8e9d95a1
172 N6d5c452329334bc88082df034dfaca65 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Molecular Sequence Data
174 rdf:type schema:DefinedTerm
175 N71ff0f9bbbb542848122b856c6ea57d2 rdf:first sg:person.0765070774.37
176 rdf:rest rdf:nil
177 N7e5a241cf30c4143b42f3e3dce32c7fc schema:volumeNumber 8
178 rdf:type schema:PublicationVolume
179 N928402f2f68647c687b69530519d2304 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Humans
181 rdf:type schema:DefinedTerm
182 N95d50053236e401eb86d250de8bc217f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
183 schema:name Epigenesis, Genetic
184 rdf:type schema:DefinedTerm
185 Naadbe578462c4d7f97051c9db4f12adc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
186 schema:name Chromosomal Proteins, Non-Histone
187 rdf:type schema:DefinedTerm
188 Nb09feccc619b41aa974063322004617c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Base Sequence
190 rdf:type schema:DefinedTerm
191 Nb32f3bcdea2c49d289824915761aaf95 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Protein Structure, Tertiary
193 rdf:type schema:DefinedTerm
194 Nb33074e2591d44d4836ca75b0f66c4a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
195 schema:name Nasopharyngeal Neoplasms
196 rdf:type schema:DefinedTerm
197 Nbf67824211dc444aac8e5f57e8218c15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name Binding Sites
199 rdf:type schema:DefinedTerm
200 Nc9bc307f69ce4caa8dcf4507c6a6796c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
201 schema:name Cytosine
202 rdf:type schema:DefinedTerm
203 Ne20870bca51e48b6b42412fb99afe440 rdf:first sg:person.0622113150.04
204 rdf:rest N54d9fe67c0c64eb38e50d36cb01085bb
205 Ne6916366d1cb430e8e324c8d1f4eb075 schema:name doi
206 schema:value 10.1186/1471-2407-8-253
207 rdf:type schema:PropertyValue
208 Ne6f6dc4858144982a9d0ed103c804b8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
209 schema:name HeLa Cells
210 rdf:type schema:DefinedTerm
211 Neb57e5576c52479f9842fbfd24573ee9 rdf:first sg:person.01262462443.74
212 rdf:rest N1d5364c2af8e478fac855762d6e508f7
213 Nf1f6958e2dc548b6b9700b2a09ba0b2a schema:issueNumber 1
214 rdf:type schema:PublicationIssue
215 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
216 schema:name Medical and Health Sciences
217 rdf:type schema:DefinedTerm
218 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
219 schema:name Oncology and Carcinogenesis
220 rdf:type schema:DefinedTerm
221 anzsrc-for:1117 schema:inDefinedTermSet anzsrc-for:
222 schema:name Public Health and Health Services
223 rdf:type schema:DefinedTerm
224 sg:grant.4902429 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2407-8-253
225 rdf:type schema:MonetaryGrant
226 sg:grant.4924628 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2407-8-253
227 rdf:type schema:MonetaryGrant
228 sg:grant.4967602 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2407-8-253
229 rdf:type schema:MonetaryGrant
230 sg:grant.4988485 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2407-8-253
231 rdf:type schema:MonetaryGrant
232 sg:journal.1024632 schema:issn 1471-2407
233 schema:name BMC Cancer
234 schema:publisher Springer Nature
235 rdf:type schema:Periodical
236 sg:person.01075456274.31 schema:affiliation grid-institutes:grid.216417.7
237 schema:familyName Zhou
238 schema:givenName Ming
239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01075456274.31
240 rdf:type schema:Person
241 sg:person.011030223667.72 schema:affiliation grid-institutes:grid.216417.7
242 schema:familyName Li
243 schema:givenName Xiayu
244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011030223667.72
245 rdf:type schema:Person
246 sg:person.01215235247.05 schema:affiliation grid-institutes:grid.216417.7
247 schema:familyName Liu
248 schema:givenName Huaying
249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215235247.05
250 rdf:type schema:Person
251 sg:person.01262462443.74 schema:affiliation grid-institutes:grid.216417.7
252 schema:familyName Niu
253 schema:givenName Zhaoxia
254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01262462443.74
255 rdf:type schema:Person
256 sg:person.0605160522.43 schema:affiliation grid-institutes:grid.216417.7
257 schema:familyName Tan
258 schema:givenName Yixin
259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605160522.43
260 rdf:type schema:Person
261 sg:person.0622113150.04 schema:affiliation grid-institutes:grid.216417.7
262 schema:familyName Deng
263 schema:givenName Tan
264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622113150.04
265 rdf:type schema:Person
266 sg:person.0715767315.55 schema:affiliation grid-institutes:grid.216417.7
267 schema:familyName Peng
268 schema:givenName Cong
269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715767315.55
270 rdf:type schema:Person
271 sg:person.0744166254.21 schema:affiliation grid-institutes:grid.216417.7
272 schema:familyName Shi
273 schema:givenName Lei
274 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744166254.21
275 rdf:type schema:Person
276 sg:person.0765070774.37 schema:affiliation grid-institutes:grid.216417.7
277 schema:familyName Li
278 schema:givenName Guiyuan
279 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765070774.37
280 rdf:type schema:Person
281 sg:person.0770421347.49 schema:affiliation grid-institutes:grid.216417.7
282 schema:familyName Zhang
283 schema:givenName Liming
284 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770421347.49
285 rdf:type schema:Person
286 sg:pub.10.1007/s11010-007-9466-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008996477
287 https://doi.org/10.1007/s11010-007-9466-x
288 rdf:type schema:CreativeWork
289 sg:pub.10.1038/labinvest.3700547 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019983752
290 https://doi.org/10.1038/labinvest.3700547
291 rdf:type schema:CreativeWork
292 sg:pub.10.1038/sj.onc.1207632 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025126353
293 https://doi.org/10.1038/sj.onc.1207632
294 rdf:type schema:CreativeWork
295 sg:pub.10.1038/sj.onc.1209839 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000270224
296 https://doi.org/10.1038/sj.onc.1209839
297 rdf:type schema:CreativeWork
298 grid-institutes:grid.216417.7 schema:alternateName Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China
299 schema:name Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, P.R. China
300 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...