DNA methylation alterations of AXIN2 in serrated adenomas and colon carcinomas with microsatellite instability View Full Text


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Article Info

DATE

2014-06-25

AUTHORS

Yuta Muto, Takafumi Maeda, Koichi Suzuki, Takaharu Kato, Fumiaki Watanabe, Hidenori Kamiyama, Masaaki Saito, Kei Koizumi, Yuichiro Miyaki, Fumio Konishi, Sergio Alonso, Manuel Perucho, Toshiki Rikiyama

ABSTRACT

BACKGROUND: Recent work led to recognize sessile serrated adenomas (SSA) as precursor to many of the sporadic colorectal cancers with microsatellite instability (MSI). However, comprehensive analyses of DNA methylation in SSA and MSI cancer have not been conducted. METHODS: With an array-based methylation sensitive amplified fragment length polymorphism (MS-AFLP) method we analyzed 8 tubular (TA) and 19 serrated (SSA) adenomas, and 14 carcinomas with (MSI) and 12 without (MSS) microsatellite instability. MS-AFLP array can survey relative differences in methylation between normal and tumor tissues of 9,654 DNA fragments containing all NotI sequences in the human genome. RESULTS: Unsupervised clustering analysis of the genome-wide hypermethylation alterations revealed no major differences between or within these groups of benign and malignant tumors regardless of their location in intergenic, intragenic, promoter, or 3' end regions. Hypomethylation was less frequent in SSAs compared with MSI or MSS carcinomas. Analysis of variance of DNA methylation between these four subgroups identified 56 probes differentially altered. The hierarchical tree of this subset of probes revealed two distinct clusters: Group 1, mostly composed by TAs and MSS cancers with KRAS mutations; and Group 2 with BRAF mutations, which consisted of cancers with MSI and MLH1 methylation (Group 2A), and SSAs without MLH1 methylation (Group 2B). AXIN2, which cooperates with APC and β-catenin in Wnt signaling, had more methylation alterations in Group 2, and its expression levels negatively correlated with methylation determined by bisulfite sequencing. Within group 2B, low and high AXIN2 expression levels correlated significantly with differences in size (P = 0.01) location (P = 0.05) and crypt architecture (P = 0.01). CONCLUSIONS: Somatic methylation alterations of AXIN2, associated with changes in its expression, stratify SSAs according to some clinico-pathological differences. We conclude that hypermethylation of MLH1, when occurs in an adenoma cell with BRAF oncogenic mutational activation, drives the pathway for MSI cancer by providing the cells with a mutator phenotype. AXIN2 inactivation may contribute to this tumorigenic pathway either by mutator phenotype driven frameshift mutations or by epigenetic deregulation contemporary with the unfolding of the mutator phenotype. More... »

PAGES

466-466

References to SciGraph publications

  • 2003-07-01. No major tumorigenic role for β-catenin in serrated as opposed to conventional colorectal adenomas in BRITISH JOURNAL OF CANCER
  • 2004-09-24. Progressive methylation during the serrated neoplasia pathway of the colorectum in MODERN PATHOLOGY
  • 1993-06. Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis in NATURE
  • 2002-02-02. Gastrointestinal cancer of the microsatellite mutator phenotype pathway in JOURNAL OF GASTROENTEROLOGY
  • 2002-06-09. Mutations of the BRAF gene in human cancer in NATURE
  • 2007-04-21. Serrated polyps of the colorectum: is sessile serrated adenoma distinguishable from hyperplastic polyp in a daily practice? in VIRCHOWS ARCHIV
  • 2000-10. Mutations in AXIN2 cause colorectal cancer with defective mismatch repair by activating β-catenin/TCF signalling in NATURE GENETICS
  • 2012-02-06. DNA demethylation in normal colon tissue predicts predisposition to multiple cancers in ONCOGENE
  • 2002-07-09. Mutational spectrum of β-catenin, AXIN1, and AXIN2 in hepatocellular carcinomas and hepatoblastomas in ONCOGENE
  • 2005-10-10. Epigenetic silencing of AXIN2 in colorectal carcinoma with microsatellite instability in ONCOGENE
  • 2010-07-09. Serrated polyps of the colon and rectum (hyperplastic polyps, sessile serrated adenomas, traditional serrated adenomas, and mixed polyps)—proposal for diagnostic criteria in VIRCHOWS ARCHIV
  • 1996-06. Microsatellite instability: The mutator that mutates the other mutator in NATURE MEDICINE
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    http://scigraph.springernature.com/pub.10.1186/1471-2407-14-466

    DOI

    http://dx.doi.org/10.1186/1471-2407-14-466

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1050356834

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24964857


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