O6-Methylguanine-DNA methyltransferase protein expression by immunohistochemistry in brain and non-brain systemic tumours: systematic review and meta-analysis of correlation with methylation-specific ... View Full Text


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Article Info

DATE

2011-01-26

AUTHORS

Marta Brell, Javier Ibáñez, Avelina Tortosa

ABSTRACT

BACKGROUND: The DNA repair protein O6-Methylguanine-DNA methyltransferase (MGMT) confers resistance to alkylating agents. Several methods have been applied to its analysis, with methylation-specific polymerase chain reaction (MSP) the most commonly used for promoter methylation study, while immunohistochemistry (IHC) has become the most frequently used for the detection of MGMT protein expression. Agreement on the best and most reliable technique for evaluating MGMT status remains unsettled. The aim of this study was to perform a systematic review and meta-analysis of the correlation between IHC and MSP. METHODS: A computer-aided search of MEDLINE (1950-October 2009), EBSCO (1966-October 2009) and EMBASE (1974-October 2009) was performed for relevant publications. Studies meeting inclusion criteria were those comparing MGMT protein expression by IHC with MGMT promoter methylation by MSP in the same cohort of patients. Methodological quality was assessed by using the QUADAS and STARD instruments. Previously published guidelines were followed for meta-analysis performance. RESULTS: Of 254 studies identified as eligible for full-text review, 52 (20.5%) met the inclusion criteria. The review showed that results of MGMT protein expression by IHC are not in close agreement with those obtained with MSP. Moreover, type of tumour (primary brain tumour vs others) was an independent covariate of accuracy estimates in the meta-regression analysis beyond the cut-off value. CONCLUSIONS: Protein expression assessed by IHC alone fails to reflect the promoter methylation status of MGMT. Thus, in attempts at clinical diagnosis the two methods seem to select different groups of patients and should not be used interchangeably. More... »

PAGES

35-35

References to SciGraph publications

  • 2002-06-11. Inactivation of O6-methylguanine-DNA methyltransferase by promoter CpG island hypermethylation in gastric cancers in BRITISH JOURNAL OF CANCER
  • 2007-01-12. MGMT prognostic impact on glioblastoma is dependent on therapeutic modalities in JOURNAL OF NEURO-ONCOLOGY
  • 1999-02. Cancer-epigenetics comes of age in NATURE GENETICS
  • 2009-12-08. MGMT promoter methylation in malignant gliomas: ready for personalized medicine? in NATURE REVIEWS NEUROLOGY
  • 2009-05-12. Long-term survivors of glioblastoma: clinical features and molecular analysis in ACTA NEUROCHIRURGICA
  • 2008-11-03. Rapid analysis of heterogeneously methylated DNA using digital methylation-sensitive high resolution melting: application to the CDKN2B (p15) gene in EPIGENETICS & CHROMATIN
  • 2005-03-31. Tumor Progression Through Epigenetic Gene Silencing of O6−Methylguanine-DNA Methyltransferase in Human Biliary Tract Cancers in ANNALS OF SURGICAL ONCOLOGY
  • 2006-07-12. Meta-DiSc: a software for meta-analysis of test accuracy data in BMC MEDICAL RESEARCH METHODOLOGY
  • 2009-04-09. Systematic reviews of diagnostic test accuracy and the Cochrane collaboration in INTERNAL AND EMERGENCY MEDICINE
  • 2006-04-07. hMLH1 and MGMT inactivation as a mechanism of tumorigenesis in monoclonal gammopathies in MODERN PATHOLOGY
  • 2007-08-13. MS-MLPA: an attractive alternative laboratory assay for robust, reliable, and semiquantitative detection of MGMT promoter hypermethylation in gliomas in LABORATORY INVESTIGATION
  • 2007-01-29. A simplified laboratory validated assay for MGMT promoter hypermethylation analysis of glioma specimens from formalin-fixed paraffin-embedded tissue in LABORATORY INVESTIGATION
  • 2007-11-15. Variation of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation in serial samples in glioblastoma in JOURNAL OF NEURO-ONCOLOGY
  • 2005-03-23. The hypermethylation and protein expression of p16 INK4A and DNA repair gene O6-methylguanine-DNA methyltransferase in various uterine cervical lesions in JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
  • 2007-10-27. Pitfalls in the assessment of MGMT expression and in its correlation with survival in diffuse astrocytomas: proposal of a feasible immunohistochemical approach in ACTA NEUROPATHOLOGICA
  • 2004-12. Immunohistochemical study for O6-methylguanine-DNA methyltransferase in the non-neoplastic and neoplastic components of gliomas in JOURNAL OF NEURO-ONCOLOGY
  • 2002-07-03. Conducting systematic reviews of diagnostic studies: didactic guidelines in BMC MEDICAL RESEARCH METHODOLOGY
  • 2003-08-02. PTEN methylation and expression in glioblastomas in ACTA NEUROPATHOLOGICA
  • 2004-04. MGMT: its role in cancer aetiology and cancer therapeutics in NATURE REVIEWS CANCER
  • 2002-08-09. Meta-analysis: Neither quick nor easy in BMC MEDICAL RESEARCH METHODOLOGY
  • 2009-10-20. Prognostic value of O6-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods in JOURNAL OF NEURO-ONCOLOGY
  • 2010-03-16. O6-Methylguanine DNA methyltransferase determined by promoter hypermethylation and immunohistochemical expression is correlated with progression-free survival in patients with glioblastoma in INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
  • 2005-10-21. DNA hypermethylation status of multiple genes in soft tissue sarcomas in MODERN PATHOLOGY
  • 2002-12-16. Frequent promoter hypermethylation of the O6-Methylguanine-DNAMethyltransferase (MGMT) gene in testicular cancer in ONCOGENE
  • 2003-11-10. The development of QUADAS: a tool for the quality assessment of studies of diagnostic accuracy included in systematic reviews in BMC MEDICAL RESEARCH METHODOLOGY
  • 2003-02-18. CpG methylation of MGMT and hMLH1 promoter in hepatocellular carcinoma associated with hepatitis viral infection in BRITISH JOURNAL OF CANCER
  • 2004-01-08. Generating mutations but providing chemosensitivity: the role of O6-methylguanine DNA methyltransferase in human cancer in ONCOGENE
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    http://scigraph.springernature.com/pub.10.1186/1471-2407-11-35

    DOI

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    DIMENSIONS

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    43 schema:description BACKGROUND: The DNA repair protein O6-Methylguanine-DNA methyltransferase (MGMT) confers resistance to alkylating agents. Several methods have been applied to its analysis, with methylation-specific polymerase chain reaction (MSP) the most commonly used for promoter methylation study, while immunohistochemistry (IHC) has become the most frequently used for the detection of MGMT protein expression. Agreement on the best and most reliable technique for evaluating MGMT status remains unsettled. The aim of this study was to perform a systematic review and meta-analysis of the correlation between IHC and MSP. METHODS: A computer-aided search of MEDLINE (1950-October 2009), EBSCO (1966-October 2009) and EMBASE (1974-October 2009) was performed for relevant publications. Studies meeting inclusion criteria were those comparing MGMT protein expression by IHC with MGMT promoter methylation by MSP in the same cohort of patients. Methodological quality was assessed by using the QUADAS and STARD instruments. Previously published guidelines were followed for meta-analysis performance. RESULTS: Of 254 studies identified as eligible for full-text review, 52 (20.5%) met the inclusion criteria. The review showed that results of MGMT protein expression by IHC are not in close agreement with those obtained with MSP. Moreover, type of tumour (primary brain tumour vs others) was an independent covariate of accuracy estimates in the meta-regression analysis beyond the cut-off value. CONCLUSIONS: Protein expression assessed by IHC alone fails to reflect the promoter methylation status of MGMT. Thus, in attempts at clinical diagnosis the two methods seem to select different groups of patients and should not be used interchangeably.
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