O6-Methylguanine-DNA methyltransferase protein expression by immunohistochemistry in brain and non-brain systemic tumours: systematic review and meta-analysis of correlation with methylation-specific ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-01-26

AUTHORS

Marta Brell, Javier Ibáñez, Avelina Tortosa

ABSTRACT

BACKGROUND: The DNA repair protein O6-Methylguanine-DNA methyltransferase (MGMT) confers resistance to alkylating agents. Several methods have been applied to its analysis, with methylation-specific polymerase chain reaction (MSP) the most commonly used for promoter methylation study, while immunohistochemistry (IHC) has become the most frequently used for the detection of MGMT protein expression. Agreement on the best and most reliable technique for evaluating MGMT status remains unsettled. The aim of this study was to perform a systematic review and meta-analysis of the correlation between IHC and MSP. METHODS: A computer-aided search of MEDLINE (1950-October 2009), EBSCO (1966-October 2009) and EMBASE (1974-October 2009) was performed for relevant publications. Studies meeting inclusion criteria were those comparing MGMT protein expression by IHC with MGMT promoter methylation by MSP in the same cohort of patients. Methodological quality was assessed by using the QUADAS and STARD instruments. Previously published guidelines were followed for meta-analysis performance. RESULTS: Of 254 studies identified as eligible for full-text review, 52 (20.5%) met the inclusion criteria. The review showed that results of MGMT protein expression by IHC are not in close agreement with those obtained with MSP. Moreover, type of tumour (primary brain tumour vs others) was an independent covariate of accuracy estimates in the meta-regression analysis beyond the cut-off value. CONCLUSIONS: Protein expression assessed by IHC alone fails to reflect the promoter methylation status of MGMT. Thus, in attempts at clinical diagnosis the two methods seem to select different groups of patients and should not be used interchangeably. More... »

PAGES

35-35

References to SciGraph publications

  • 2002-06-11. Inactivation of O6-methylguanine-DNA methyltransferase by promoter CpG island hypermethylation in gastric cancers in BRITISH JOURNAL OF CANCER
  • 2007-01-12. MGMT prognostic impact on glioblastoma is dependent on therapeutic modalities in JOURNAL OF NEURO-ONCOLOGY
  • 1999-02. Cancer-epigenetics comes of age in NATURE GENETICS
  • 2009-12-08. MGMT promoter methylation in malignant gliomas: ready for personalized medicine? in NATURE REVIEWS NEUROLOGY
  • 2009-05-12. Long-term survivors of glioblastoma: clinical features and molecular analysis in ACTA NEUROCHIRURGICA
  • 2008-11-03. Rapid analysis of heterogeneously methylated DNA using digital methylation-sensitive high resolution melting: application to the CDKN2B (p15) gene in EPIGENETICS & CHROMATIN
  • 2005-03-31. Tumor Progression Through Epigenetic Gene Silencing of O6−Methylguanine-DNA Methyltransferase in Human Biliary Tract Cancers in ANNALS OF SURGICAL ONCOLOGY
  • 2006-07-12. Meta-DiSc: a software for meta-analysis of test accuracy data in BMC MEDICAL RESEARCH METHODOLOGY
  • 2009-04-09. Systematic reviews of diagnostic test accuracy and the Cochrane collaboration in INTERNAL AND EMERGENCY MEDICINE
  • 2006-04-07. hMLH1 and MGMT inactivation as a mechanism of tumorigenesis in monoclonal gammopathies in MODERN PATHOLOGY
  • 2007-08-13. MS-MLPA: an attractive alternative laboratory assay for robust, reliable, and semiquantitative detection of MGMT promoter hypermethylation in gliomas in LABORATORY INVESTIGATION
  • 2007-01-29. A simplified laboratory validated assay for MGMT promoter hypermethylation analysis of glioma specimens from formalin-fixed paraffin-embedded tissue in LABORATORY INVESTIGATION
  • 2007-11-15. Variation of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation in serial samples in glioblastoma in JOURNAL OF NEURO-ONCOLOGY
  • 2005-03-23. The hypermethylation and protein expression of p16 INK4A and DNA repair gene O6-methylguanine-DNA methyltransferase in various uterine cervical lesions in JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
  • 2007-10-27. Pitfalls in the assessment of MGMT expression and in its correlation with survival in diffuse astrocytomas: proposal of a feasible immunohistochemical approach in ACTA NEUROPATHOLOGICA
  • 2004-12. Immunohistochemical study for O6-methylguanine-DNA methyltransferase in the non-neoplastic and neoplastic components of gliomas in JOURNAL OF NEURO-ONCOLOGY
  • 2002-07-03. Conducting systematic reviews of diagnostic studies: didactic guidelines in BMC MEDICAL RESEARCH METHODOLOGY
  • 2003-08-02. PTEN methylation and expression in glioblastomas in ACTA NEUROPATHOLOGICA
  • 2004-04. MGMT: its role in cancer aetiology and cancer therapeutics in NATURE REVIEWS CANCER
  • 2002-08-09. Meta-analysis: Neither quick nor easy in BMC MEDICAL RESEARCH METHODOLOGY
  • 2009-10-20. Prognostic value of O6-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods in JOURNAL OF NEURO-ONCOLOGY
  • 2010-03-16. O6-Methylguanine DNA methyltransferase determined by promoter hypermethylation and immunohistochemical expression is correlated with progression-free survival in patients with glioblastoma in INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
  • 2005-10-21. DNA hypermethylation status of multiple genes in soft tissue sarcomas in MODERN PATHOLOGY
  • 2002-12-16. Frequent promoter hypermethylation of the O6-Methylguanine-DNAMethyltransferase (MGMT) gene in testicular cancer in ONCOGENE
  • 2003-11-10. The development of QUADAS: a tool for the quality assessment of studies of diagnostic accuracy included in systematic reviews in BMC MEDICAL RESEARCH METHODOLOGY
  • 2003-02-18. CpG methylation of MGMT and hMLH1 promoter in hepatocellular carcinoma associated with hepatitis viral infection in BRITISH JOURNAL OF CANCER
  • 2004-01-08. Generating mutations but providing chemosensitivity: the role of O6-methylguanine DNA methyltransferase in human cancer in ONCOGENE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1471-2407-11-35

    DOI

    http://dx.doi.org/10.1186/1471-2407-11-35

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028492305

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21269507


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Oncology and Carcinogenesis", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1117", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Public Health and Health Services", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Brain Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Methylation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immunohistochemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "O(6)-Methylguanine-DNA Methyltransferase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Prognosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Promoter Regions, Genetic", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Neurosurgery, Son Dureta University Hospital, Palma de Mallorca, Spain", 
              "id": "http://www.grid.ac/institutes/grid.411161.2", 
              "name": [
                "Department of Neurosurgery, Son Dureta University Hospital, Palma de Mallorca, Spain"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Brell", 
            "givenName": "Marta", 
            "id": "sg:person.0640456517.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640456517.25"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Neurosurgery, Son Dureta University Hospital, Palma de Mallorca, Spain", 
              "id": "http://www.grid.ac/institutes/grid.411161.2", 
              "name": [
                "Department of Neurosurgery, Son Dureta University Hospital, Palma de Mallorca, Spain"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ib\u00e1\u00f1ez", 
            "givenName": "Javier", 
            "id": "sg:person.07527660632.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07527660632.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Basic Nursing, IDIBELL-Universitat de Barcelona, L'Hospitalet de Llobregat, Spain", 
              "id": "http://www.grid.ac/institutes/grid.5841.8", 
              "name": [
                "Department of Basic Nursing, IDIBELL-Universitat de Barcelona, L'Hospitalet de Llobregat, Spain"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tortosa", 
            "givenName": "Avelina", 
            "id": "sg:person.01064132370.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064132370.31"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1186/1471-2288-6-31", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039441705", 
              "https://doi.org/10.1186/1471-2288-6-31"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/modpathol.3800590", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047636030", 
              "https://doi.org/10.1038/modpathol.3800590"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1207316", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038974145", 
              "https://doi.org/10.1038/sj.onc.1207316"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11739-009-0243-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024738208", 
              "https://doi.org/10.1007/s11739-009-0243-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1319", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001422633", 
              "https://doi.org/10.1038/nrc1319"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1205978", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040511970", 
              "https://doi.org/10.1038/sj.onc.1205978"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00432-004-0657-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045095806", 
              "https://doi.org/10.1007/s00432-004-0657-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11060-009-0031-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000352820", 
              "https://doi.org/10.1007/s11060-009-0031-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1245/aso.2005.07.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017242659", 
              "https://doi.org/10.1245/aso.2005.07.020"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11060-007-9486-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000356397", 
              "https://doi.org/10.1007/s11060-007-9486-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/modpathol.3800502", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010630963", 
              "https://doi.org/10.1038/modpathol.3800502"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00401-007-0310-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006960609", 
              "https://doi.org/10.1007/s00401-007-0310-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/5947", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033054502", 
              "https://doi.org/10.1038/5947"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2288-2-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035579835", 
              "https://doi.org/10.1186/1471-2288-2-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/labinvest.3700520", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023008564", 
              "https://doi.org/10.1038/labinvest.3700520"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.bjc.6600743", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034710424", 
              "https://doi.org/10.1038/sj.bjc.6600743"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1756-8935-1-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051126796", 
              "https://doi.org/10.1186/1756-8935-1-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2288-3-25", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048548616", 
              "https://doi.org/10.1186/1471-2288-3-25"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/labinvest.3700664", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019648480", 
              "https://doi.org/10.1038/labinvest.3700664"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11060-006-9320-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037290910", 
              "https://doi.org/10.1007/s11060-006-9320-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.bjc.6600372", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050784282", 
              "https://doi.org/10.1038/sj.bjc.6600372"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10147-010-0065-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014108305", 
              "https://doi.org/10.1007/s10147-010-0065-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2288-2-10", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007168818", 
              "https://doi.org/10.1186/1471-2288-2-10"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrneurol.2009.197", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046540128", 
              "https://doi.org/10.1038/nrneurol.2009.197"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00401-003-0748-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003556047", 
              "https://doi.org/10.1007/s00401-003-0748-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00701-009-0387-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026556660", 
              "https://doi.org/10.1007/s00701-009-0387-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11060-004-9170-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036276006", 
              "https://doi.org/10.1007/s11060-004-9170-6"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2011-01-26", 
        "datePublishedReg": "2011-01-26", 
        "description": "BACKGROUND: The DNA repair protein O6-Methylguanine-DNA methyltransferase (MGMT) confers resistance to alkylating agents. Several methods have been applied to its analysis, with methylation-specific polymerase chain reaction (MSP) the most commonly used for promoter methylation study, while immunohistochemistry (IHC) has become the most frequently used for the detection of MGMT protein expression. Agreement on the best and most reliable technique for evaluating MGMT status remains unsettled. The aim of this study was to perform a systematic review and meta-analysis of the correlation between IHC and MSP.\nMETHODS: A computer-aided search of MEDLINE (1950-October 2009), EBSCO (1966-October 2009) and EMBASE (1974-October 2009) was performed for relevant publications. Studies meeting inclusion criteria were those comparing MGMT protein expression by IHC with MGMT promoter methylation by MSP in the same cohort of patients. Methodological quality was assessed by using the QUADAS and STARD instruments. Previously published guidelines were followed for meta-analysis performance.\nRESULTS: Of 254 studies identified as eligible for full-text review, 52 (20.5%) met the inclusion criteria. The review showed that results of MGMT protein expression by IHC are not in close agreement with those obtained with MSP. Moreover, type of tumour (primary brain tumour vs others) was an independent covariate of accuracy estimates in the meta-regression analysis beyond the cut-off value.\nCONCLUSIONS: Protein expression assessed by IHC alone fails to reflect the promoter methylation status of MGMT. Thus, in attempts at clinical diagnosis the two methods seem to select different groups of patients and should not be used interchangeably.", 
        "genre": "article", 
        "id": "sg:pub.10.1186/1471-2407-11-35", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1024632", 
            "issn": [
              "1471-2407"
            ], 
            "name": "BMC Cancer", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "11"
          }
        ], 
        "keywords": [
          "methylation-specific polymerase chain reaction", 
          "MGMT protein expression", 
          "protein expression", 
          "polymerase chain reaction", 
          "inclusion criteria", 
          "systematic review", 
          "chain reaction", 
          "types of tumors", 
          "full-text review", 
          "MGMT promoter methylation", 
          "meta-regression analysis", 
          "meta-analysis performance", 
          "DNA methyltransferase protein expression", 
          "promoter methylation status", 
          "systemic tumors", 
          "computer-aided search", 
          "O6-methylguanine", 
          "methodological quality", 
          "immunohistochemistry", 
          "MGMT status", 
          "clinical diagnosis", 
          "same cohort", 
          "independent covariates", 
          "promoter methylation studies", 
          "promoter methylation", 
          "relevant publications", 
          "patients", 
          "tumors", 
          "methylation status", 
          "review", 
          "expression", 
          "reliable technique", 
          "status", 
          "EMBASE", 
          "MEDLINE", 
          "cohort", 
          "study", 
          "different groups", 
          "diagnosis", 
          "brain", 
          "DNA methyltransferase", 
          "EBSCO", 
          "QUADAS", 
          "MGMT", 
          "methylation studies", 
          "criteria", 
          "covariates", 
          "correlation", 
          "guidelines", 
          "group", 
          "aim", 
          "agents", 
          "methyltransferase", 
          "resistance", 
          "analysis", 
          "methylation", 
          "search", 
          "reaction", 
          "publications", 
          "quality", 
          "accuracy estimates", 
          "detection", 
          "types", 
          "method", 
          "instrument", 
          "results", 
          "attempt", 
          "values", 
          "technique", 
          "estimates", 
          "agreement", 
          "close agreement", 
          "performance", 
          "DNA repair protein O6-Methylguanine", 
          "repair protein O6-Methylguanine", 
          "protein O6-Methylguanine", 
          "STARD instruments", 
          "methyltransferase protein expression", 
          "non-brain systemic tumours"
        ], 
        "name": "O6-Methylguanine-DNA methyltransferase protein expression by immunohistochemistry in brain and non-brain systemic tumours: systematic review and meta-analysis of correlation with methylation-specific polymerase chain reaction", 
        "pagination": "35-35", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1028492305"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/1471-2407-11-35"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "21269507"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/1471-2407-11-35", 
          "https://app.dimensions.ai/details/publication/pub.1028492305"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2021-12-01T19:26", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_553.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1186/1471-2407-11-35"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-11-35'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-11-35'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-11-35'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-11-35'


     

    This table displays all metadata directly associated to this object as RDF triples.

    305 TRIPLES      22 PREDICATES      142 URIs      106 LITERALS      16 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/1471-2407-11-35 schema:about N2e49db7cba174bd4b9e08774a8b07cf1
    2 N2effca73403c4f81b24f205fb4c393cb
    3 N469e2e01af98431c982d0a2133834f8b
    4 N63a8a94f0d6a4da291eab736ac2f0028
    5 N7b65e629cae8473c95777e3f578ac6e9
    6 N8082ccb64206412083370e4d8954df40
    7 N80ff065696464321a20b78404a8ec921
    8 N868ab520b46a4a71ad03ecb3671f68b0
    9 Nda7987789a434aa4a6b11d4bf6d1a02a
    10 anzsrc-for:11
    11 anzsrc-for:1112
    12 anzsrc-for:1117
    13 schema:author N9b94e8f682be45889680bbe70cca209e
    14 schema:citation sg:pub.10.1007/s00401-003-0748-4
    15 sg:pub.10.1007/s00401-007-0310-x
    16 sg:pub.10.1007/s00432-004-0657-5
    17 sg:pub.10.1007/s00701-009-0387-1
    18 sg:pub.10.1007/s10147-010-0065-6
    19 sg:pub.10.1007/s11060-004-9170-6
    20 sg:pub.10.1007/s11060-006-9320-0
    21 sg:pub.10.1007/s11060-007-9486-0
    22 sg:pub.10.1007/s11060-009-0031-1
    23 sg:pub.10.1007/s11739-009-0243-6
    24 sg:pub.10.1038/5947
    25 sg:pub.10.1038/labinvest.3700520
    26 sg:pub.10.1038/labinvest.3700664
    27 sg:pub.10.1038/modpathol.3800502
    28 sg:pub.10.1038/modpathol.3800590
    29 sg:pub.10.1038/nrc1319
    30 sg:pub.10.1038/nrneurol.2009.197
    31 sg:pub.10.1038/sj.bjc.6600372
    32 sg:pub.10.1038/sj.bjc.6600743
    33 sg:pub.10.1038/sj.onc.1205978
    34 sg:pub.10.1038/sj.onc.1207316
    35 sg:pub.10.1186/1471-2288-2-10
    36 sg:pub.10.1186/1471-2288-2-9
    37 sg:pub.10.1186/1471-2288-3-25
    38 sg:pub.10.1186/1471-2288-6-31
    39 sg:pub.10.1186/1756-8935-1-7
    40 sg:pub.10.1245/aso.2005.07.020
    41 schema:datePublished 2011-01-26
    42 schema:datePublishedReg 2011-01-26
    43 schema:description BACKGROUND: The DNA repair protein O6-Methylguanine-DNA methyltransferase (MGMT) confers resistance to alkylating agents. Several methods have been applied to its analysis, with methylation-specific polymerase chain reaction (MSP) the most commonly used for promoter methylation study, while immunohistochemistry (IHC) has become the most frequently used for the detection of MGMT protein expression. Agreement on the best and most reliable technique for evaluating MGMT status remains unsettled. The aim of this study was to perform a systematic review and meta-analysis of the correlation between IHC and MSP. METHODS: A computer-aided search of MEDLINE (1950-October 2009), EBSCO (1966-October 2009) and EMBASE (1974-October 2009) was performed for relevant publications. Studies meeting inclusion criteria were those comparing MGMT protein expression by IHC with MGMT promoter methylation by MSP in the same cohort of patients. Methodological quality was assessed by using the QUADAS and STARD instruments. Previously published guidelines were followed for meta-analysis performance. RESULTS: Of 254 studies identified as eligible for full-text review, 52 (20.5%) met the inclusion criteria. The review showed that results of MGMT protein expression by IHC are not in close agreement with those obtained with MSP. Moreover, type of tumour (primary brain tumour vs others) was an independent covariate of accuracy estimates in the meta-regression analysis beyond the cut-off value. CONCLUSIONS: Protein expression assessed by IHC alone fails to reflect the promoter methylation status of MGMT. Thus, in attempts at clinical diagnosis the two methods seem to select different groups of patients and should not be used interchangeably.
    44 schema:genre article
    45 schema:inLanguage en
    46 schema:isAccessibleForFree true
    47 schema:isPartOf N53b39c2e26e649ba82d73c015e389dd4
    48 Naea39b85616f4a2990fae15be7da5aa5
    49 sg:journal.1024632
    50 schema:keywords DNA methyltransferase
    51 DNA methyltransferase protein expression
    52 DNA repair protein O6-Methylguanine
    53 EBSCO
    54 EMBASE
    55 MEDLINE
    56 MGMT
    57 MGMT promoter methylation
    58 MGMT protein expression
    59 MGMT status
    60 O6-methylguanine
    61 QUADAS
    62 STARD instruments
    63 accuracy estimates
    64 agents
    65 agreement
    66 aim
    67 analysis
    68 attempt
    69 brain
    70 chain reaction
    71 clinical diagnosis
    72 close agreement
    73 cohort
    74 computer-aided search
    75 correlation
    76 covariates
    77 criteria
    78 detection
    79 diagnosis
    80 different groups
    81 estimates
    82 expression
    83 full-text review
    84 group
    85 guidelines
    86 immunohistochemistry
    87 inclusion criteria
    88 independent covariates
    89 instrument
    90 meta-analysis performance
    91 meta-regression analysis
    92 method
    93 methodological quality
    94 methylation
    95 methylation status
    96 methylation studies
    97 methylation-specific polymerase chain reaction
    98 methyltransferase
    99 methyltransferase protein expression
    100 non-brain systemic tumours
    101 patients
    102 performance
    103 polymerase chain reaction
    104 promoter methylation
    105 promoter methylation status
    106 promoter methylation studies
    107 protein O6-Methylguanine
    108 protein expression
    109 publications
    110 quality
    111 reaction
    112 relevant publications
    113 reliable technique
    114 repair protein O6-Methylguanine
    115 resistance
    116 results
    117 review
    118 same cohort
    119 search
    120 status
    121 study
    122 systematic review
    123 systemic tumors
    124 technique
    125 tumors
    126 types
    127 types of tumors
    128 values
    129 schema:name O6-Methylguanine-DNA methyltransferase protein expression by immunohistochemistry in brain and non-brain systemic tumours: systematic review and meta-analysis of correlation with methylation-specific polymerase chain reaction
    130 schema:pagination 35-35
    131 schema:productId N101040de624d41188ff2a493e9aa88d7
    132 N610f336cf9b549ed9f1acbe772345182
    133 Nd941934900ca45948ee74078a0a19698
    134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028492305
    135 https://doi.org/10.1186/1471-2407-11-35
    136 schema:sdDatePublished 2021-12-01T19:26
    137 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    138 schema:sdPublisher Nd3dcf5cf054d4a33846923a0edaa6c58
    139 schema:url https://doi.org/10.1186/1471-2407-11-35
    140 sgo:license sg:explorer/license/
    141 sgo:sdDataset articles
    142 rdf:type schema:ScholarlyArticle
    143 N101040de624d41188ff2a493e9aa88d7 schema:name dimensions_id
    144 schema:value pub.1028492305
    145 rdf:type schema:PropertyValue
    146 N2e49db7cba174bd4b9e08774a8b07cf1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name O(6)-Methylguanine-DNA Methyltransferase
    148 rdf:type schema:DefinedTerm
    149 N2effca73403c4f81b24f205fb4c393cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Brain Neoplasms
    151 rdf:type schema:DefinedTerm
    152 N469e2e01af98431c982d0a2133834f8b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Polymerase Chain Reaction
    154 rdf:type schema:DefinedTerm
    155 N53b39c2e26e649ba82d73c015e389dd4 schema:issueNumber 1
    156 rdf:type schema:PublicationIssue
    157 N610f336cf9b549ed9f1acbe772345182 schema:name pubmed_id
    158 schema:value 21269507
    159 rdf:type schema:PropertyValue
    160 N63a8a94f0d6a4da291eab736ac2f0028 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name Promoter Regions, Genetic
    162 rdf:type schema:DefinedTerm
    163 N7b65e629cae8473c95777e3f578ac6e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name DNA Methylation
    165 rdf:type schema:DefinedTerm
    166 N8082ccb64206412083370e4d8954df40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    167 schema:name Immunohistochemistry
    168 rdf:type schema:DefinedTerm
    169 N80ff065696464321a20b78404a8ec921 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    170 schema:name Humans
    171 rdf:type schema:DefinedTerm
    172 N81f560a67525434fb779ede4df525ffa rdf:first sg:person.07527660632.32
    173 rdf:rest N8e5125171c2f46ef9a097c7c61dce9e2
    174 N868ab520b46a4a71ad03ecb3671f68b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    175 schema:name Prognosis
    176 rdf:type schema:DefinedTerm
    177 N8e5125171c2f46ef9a097c7c61dce9e2 rdf:first sg:person.01064132370.31
    178 rdf:rest rdf:nil
    179 N9b94e8f682be45889680bbe70cca209e rdf:first sg:person.0640456517.25
    180 rdf:rest N81f560a67525434fb779ede4df525ffa
    181 Naea39b85616f4a2990fae15be7da5aa5 schema:volumeNumber 11
    182 rdf:type schema:PublicationVolume
    183 Nd3dcf5cf054d4a33846923a0edaa6c58 schema:name Springer Nature - SN SciGraph project
    184 rdf:type schema:Organization
    185 Nd941934900ca45948ee74078a0a19698 schema:name doi
    186 schema:value 10.1186/1471-2407-11-35
    187 rdf:type schema:PropertyValue
    188 Nda7987789a434aa4a6b11d4bf6d1a02a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    189 schema:name Neoplasms
    190 rdf:type schema:DefinedTerm
    191 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    192 schema:name Medical and Health Sciences
    193 rdf:type schema:DefinedTerm
    194 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
    195 schema:name Oncology and Carcinogenesis
    196 rdf:type schema:DefinedTerm
    197 anzsrc-for:1117 schema:inDefinedTermSet anzsrc-for:
    198 schema:name Public Health and Health Services
    199 rdf:type schema:DefinedTerm
    200 sg:journal.1024632 schema:issn 1471-2407
    201 schema:name BMC Cancer
    202 schema:publisher Springer Nature
    203 rdf:type schema:Periodical
    204 sg:person.01064132370.31 schema:affiliation grid-institutes:grid.5841.8
    205 schema:familyName Tortosa
    206 schema:givenName Avelina
    207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064132370.31
    208 rdf:type schema:Person
    209 sg:person.0640456517.25 schema:affiliation grid-institutes:grid.411161.2
    210 schema:familyName Brell
    211 schema:givenName Marta
    212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640456517.25
    213 rdf:type schema:Person
    214 sg:person.07527660632.32 schema:affiliation grid-institutes:grid.411161.2
    215 schema:familyName Ibáñez
    216 schema:givenName Javier
    217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07527660632.32
    218 rdf:type schema:Person
    219 sg:pub.10.1007/s00401-003-0748-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003556047
    220 https://doi.org/10.1007/s00401-003-0748-4
    221 rdf:type schema:CreativeWork
    222 sg:pub.10.1007/s00401-007-0310-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006960609
    223 https://doi.org/10.1007/s00401-007-0310-x
    224 rdf:type schema:CreativeWork
    225 sg:pub.10.1007/s00432-004-0657-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045095806
    226 https://doi.org/10.1007/s00432-004-0657-5
    227 rdf:type schema:CreativeWork
    228 sg:pub.10.1007/s00701-009-0387-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026556660
    229 https://doi.org/10.1007/s00701-009-0387-1
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.1007/s10147-010-0065-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014108305
    232 https://doi.org/10.1007/s10147-010-0065-6
    233 rdf:type schema:CreativeWork
    234 sg:pub.10.1007/s11060-004-9170-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036276006
    235 https://doi.org/10.1007/s11060-004-9170-6
    236 rdf:type schema:CreativeWork
    237 sg:pub.10.1007/s11060-006-9320-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037290910
    238 https://doi.org/10.1007/s11060-006-9320-0
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1007/s11060-007-9486-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000356397
    241 https://doi.org/10.1007/s11060-007-9486-0
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1007/s11060-009-0031-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000352820
    244 https://doi.org/10.1007/s11060-009-0031-1
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1007/s11739-009-0243-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024738208
    247 https://doi.org/10.1007/s11739-009-0243-6
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1038/5947 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033054502
    250 https://doi.org/10.1038/5947
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1038/labinvest.3700520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023008564
    253 https://doi.org/10.1038/labinvest.3700520
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1038/labinvest.3700664 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019648480
    256 https://doi.org/10.1038/labinvest.3700664
    257 rdf:type schema:CreativeWork
    258 sg:pub.10.1038/modpathol.3800502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010630963
    259 https://doi.org/10.1038/modpathol.3800502
    260 rdf:type schema:CreativeWork
    261 sg:pub.10.1038/modpathol.3800590 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047636030
    262 https://doi.org/10.1038/modpathol.3800590
    263 rdf:type schema:CreativeWork
    264 sg:pub.10.1038/nrc1319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001422633
    265 https://doi.org/10.1038/nrc1319
    266 rdf:type schema:CreativeWork
    267 sg:pub.10.1038/nrneurol.2009.197 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046540128
    268 https://doi.org/10.1038/nrneurol.2009.197
    269 rdf:type schema:CreativeWork
    270 sg:pub.10.1038/sj.bjc.6600372 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050784282
    271 https://doi.org/10.1038/sj.bjc.6600372
    272 rdf:type schema:CreativeWork
    273 sg:pub.10.1038/sj.bjc.6600743 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034710424
    274 https://doi.org/10.1038/sj.bjc.6600743
    275 rdf:type schema:CreativeWork
    276 sg:pub.10.1038/sj.onc.1205978 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040511970
    277 https://doi.org/10.1038/sj.onc.1205978
    278 rdf:type schema:CreativeWork
    279 sg:pub.10.1038/sj.onc.1207316 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038974145
    280 https://doi.org/10.1038/sj.onc.1207316
    281 rdf:type schema:CreativeWork
    282 sg:pub.10.1186/1471-2288-2-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007168818
    283 https://doi.org/10.1186/1471-2288-2-10
    284 rdf:type schema:CreativeWork
    285 sg:pub.10.1186/1471-2288-2-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035579835
    286 https://doi.org/10.1186/1471-2288-2-9
    287 rdf:type schema:CreativeWork
    288 sg:pub.10.1186/1471-2288-3-25 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048548616
    289 https://doi.org/10.1186/1471-2288-3-25
    290 rdf:type schema:CreativeWork
    291 sg:pub.10.1186/1471-2288-6-31 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039441705
    292 https://doi.org/10.1186/1471-2288-6-31
    293 rdf:type schema:CreativeWork
    294 sg:pub.10.1186/1756-8935-1-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051126796
    295 https://doi.org/10.1186/1756-8935-1-7
    296 rdf:type schema:CreativeWork
    297 sg:pub.10.1245/aso.2005.07.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017242659
    298 https://doi.org/10.1245/aso.2005.07.020
    299 rdf:type schema:CreativeWork
    300 grid-institutes:grid.411161.2 schema:alternateName Department of Neurosurgery, Son Dureta University Hospital, Palma de Mallorca, Spain
    301 schema:name Department of Neurosurgery, Son Dureta University Hospital, Palma de Mallorca, Spain
    302 rdf:type schema:Organization
    303 grid-institutes:grid.5841.8 schema:alternateName Department of Basic Nursing, IDIBELL-Universitat de Barcelona, L'Hospitalet de Llobregat, Spain
    304 schema:name Department of Basic Nursing, IDIBELL-Universitat de Barcelona, L'Hospitalet de Llobregat, Spain
    305 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...