Fast growth associated with aberrant vasculature and hypoxia in fibroblast growth factor 8b (FGF8b) over-expressing PC-3 prostate tumour xenografts View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-10-30

AUTHORS

Johanna Tuomela, Tove J Grönroos, Maija P Valta, Jouko Sandholm, Aleksi Schrey, Jani Seppänen, Päivi Marjamäki, Sarita Forsback, Ilpo Kinnunen, Olof Solin, Heikki Minn, Pirkko L Härkönen

ABSTRACT

BackgroundProstate tumours are commonly poorly oxygenated which is associated with tumour progression and development of resistance to chemotherapeutic drugs and radiotherapy. Fibroblast growth factor 8b (FGF8b) is a mitogenic and angiogenic factor, which is expressed at an increased level in human prostate tumours and is associated with a poor prognosis. We studied the effect of FGF8b on tumour oxygenation and growth parameters in xenografts in comparison with vascular endothelial growth factor (VEGF)-expressing xenografts, representing another fast growing and angiogenic tumour model.MethodsSubcutaneous tumours of PC-3 cells transfected with FGF8b, VEGF or empty (mock) vectors were produced and studied for vascularity, cell proliferation, glucose metabolism and oxygenation. Tumours were evaluated by immunohistochemistry (IHC), flow cytometry, use of radiolabelled markers of energy metabolism ([18F]FDG) and hypoxia ([18F]EF5), and intratumoral polarographic measurements of pO2.ResultsBoth FGF8b and VEGF tumours grew rapidly in nude mice and showed highly vascularised morphology. Perfusion studies, pO2 measurements, [18F]EF5 and [18F]FDG uptake as well as IHC staining for glucose transport protein (GLUT1) and hypoxia inducible factor (HIF) 1 showed that VEGF xenografts were well-perfused and oxygenised, as expected, whereas FGF8b tumours were as hypoxic as mock tumours. These results suggest that FGF8b-induced tumour capillaries are defective. Nevertheless, the growth rate of hypoxic FGF8b tumours was highly increased, as that of well-oxygenised VEGF tumours, when compared with hypoxic mock tumour controls.ConclusionFGF8b is able to induce fast growth in strongly hypoxic tumour microenvironment whereas VEGF-stimulated growth advantage is associated with improved perfusion and oxygenation of prostate tumour xenografts. More... »

PAGES

596

References to SciGraph publications

  • 2008-05-13. Quantitative techniques in 18FDG PET scanning in oncology in BRITISH JOURNAL OF CANCER
  • 2002-01-01. Hypoxia — a key regulatory factor in tumour growth in NATURE REVIEWS CANCER
  • 1996-04. Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene in NATURE
  • 2001-06-01. Increased Expression of FGF-8 Isoforms and FGF Receptors in Human Premalignant Prostatic Intraepithelial Neoplasia Lesions and Prostate Cancer in LABORATORY INVESTIGATION
  • 2008-03-28. Alendronate decreases orthotopic PC-3 prostate tumor growth and metastasis to prostate-draining lymph nodes in nude mice in BMC CANCER
  • 2004-11. Why do cancers have high aerobic glycolysis? in NATURE REVIEWS CANCER
  • 2003-03-31. Angiogenic synergism, vascular stability and improvement of hind-limb ischemia by a combination of PDGF-BB and FGF-2 in NATURE MEDICINE
  • 2001-08-14. Correlation of vascular endothelial growth factor expression with fibroblast growth factor-8 expression and clinico-pathologic parameters in human prostate cancer in BRITISH JOURNAL OF CANCER
  • 2001-05-17. FGF-8b increases angiogenic capacity and tumor growth of androgen-regulated S115 breast cancer cells in ONCOGENE
  • 1998-03-19. Downregulation of human FGF8 activity by antisense constructs in murine fibroblastic and human prostatic carcinoma cell systems in ONCOGENE
  • 2003-04-29. FGF8 isoform b expression in human prostate cancer in BRITISH JOURNAL OF CANCER
  • 2004-06-01. Exploiting tumour hypoxia in cancer treatment in NATURE REVIEWS CANCER
  • 2004-01-14. Comparison of the biodistribution of two hypoxia markers [18F]FETNIM and [18F]FMISO in an experimental mammary carcinoma in EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
  • 1999-04-28. FGF8 over-expression in prostate cancer is associated with decreased patient survival and persists in androgen independent disease in ONCOGENE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1471-2407-10-596

    DOI

    http://dx.doi.org/10.1186/1471-2407-10-596

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1044857053

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21034500


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Oncology and Carcinogenesis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fibroblast Growth Factor 8", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation, Neoplastic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hypoxia", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Nude", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neoplasm Transplantation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oxygen", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Prognosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Prostatic Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transfection", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Vascular Endothelial Growth Factor A", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Pharmatest Services Ltd., Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.452714.1", 
              "name": [
                "Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, Turku, Finland", 
                "Pharmatest Services Ltd., Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tuomela", 
            "givenName": "Johanna", 
            "id": "sg:person.01244231205.07", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244231205.07"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Turku PET Centre, MediCity Preclinical Research Laboratory, University of Turku and \u00c5bo Akademi University, Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.470895.7", 
              "name": [
                "Turku PET Centre, MediCity Preclinical Research Laboratory, University of Turku and \u00c5bo Akademi University, Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gr\u00f6nroos", 
            "givenName": "Tove J", 
            "id": "sg:person.01102756462.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102756462.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.1374.1", 
              "name": [
                "Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Valta", 
            "givenName": "Maija P", 
            "id": "sg:person.01176116005.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01176116005.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Cell Imaging Core, Turku Centre for Biotechnology, University of Turku and \u00c5bo Akademi University, Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.452861.c", 
              "name": [
                "Cell Imaging Core, Turku Centre for Biotechnology, University of Turku and \u00c5bo Akademi University, Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sandholm", 
            "givenName": "Jouko", 
            "id": "sg:person.0722714143.14", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722714143.14"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.1374.1", 
              "name": [
                "Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schrey", 
            "givenName": "Aleksi", 
            "id": "sg:person.01221116075.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221116075.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pharmatest Services Ltd., Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.452714.1", 
              "name": [
                "Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, Turku, Finland", 
                "Pharmatest Services Ltd., Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sepp\u00e4nen", 
            "givenName": "Jani", 
            "id": "sg:person.01050347723.38", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050347723.38"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Turku PET Centre, MediCity Preclinical Research Laboratory, University of Turku and \u00c5bo Akademi University, Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.470895.7", 
              "name": [
                "Turku PET Centre, MediCity Preclinical Research Laboratory, University of Turku and \u00c5bo Akademi University, Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Marjam\u00e4ki", 
            "givenName": "P\u00e4ivi", 
            "id": "sg:person.01057277261.68", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057277261.68"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Turku PET Centre, Radiopharmaceutical Chemistry Laboratory, University of Turku and \u00c5bo Akademi University, Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.470895.7", 
              "name": [
                "Turku PET Centre, Radiopharmaceutical Chemistry Laboratory, University of Turku and \u00c5bo Akademi University, Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Forsback", 
            "givenName": "Sarita", 
            "id": "sg:person.01071362053.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071362053.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.1374.1", 
              "name": [
                "Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kinnunen", 
            "givenName": "Ilpo", 
            "id": "sg:person.01335344475.48", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335344475.48"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Turku PET Centre, Accelerator Laboratory, MediCity Preclinical Research Laboratory, University of Turku and \u00c5bo Akademi University, Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.470895.7", 
              "name": [
                "Turku PET Centre, Accelerator Laboratory, MediCity Preclinical Research Laboratory, University of Turku and \u00c5bo Akademi University, Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Solin", 
            "givenName": "Olof", 
            "id": "sg:person.0656631675.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656631675.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Oncology and Radiotherapy, Turku University Hospital, Turku, Finland", 
              "id": "http://www.grid.ac/institutes/grid.410552.7", 
              "name": [
                "Department of Oncology and Radiotherapy, Turku University Hospital, Turku, Finland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Minn", 
            "givenName": "Heikki", 
            "id": "sg:person.0700413711.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700413711.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Laboratory Medicine, MAS University Hospital, Lund University, Malm\u00f6, Sweden", 
              "id": "http://www.grid.ac/institutes/grid.4514.4", 
              "name": [
                "Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, Turku, Finland", 
                "Department of Laboratory Medicine, MAS University Hospital, Lund University, Malm\u00f6, Sweden"
              ], 
              "type": "Organization"
            }, 
            "familyName": "H\u00e4rk\u00f6nen", 
            "givenName": "Pirkko L", 
            "id": "sg:person.01131663575.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131663575.79"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1186/1471-2407-8-81", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009684555", 
              "https://doi.org/10.1186/1471-2407-8-81"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc704", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028740665", 
              "https://doi.org/10.1038/nrc704"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1204430", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024680700", 
              "https://doi.org/10.1038/sj.onc.1204430"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.bjc.6604330", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031788073", 
              "https://doi.org/10.1038/sj.bjc.6604330"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1478", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032257073", 
              "https://doi.org/10.1038/nrc1478"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1201652", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025156232", 
              "https://doi.org/10.1038/sj.onc.1201652"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00259-003-1404-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019896114", 
              "https://doi.org/10.1007/s00259-003-1404-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1367", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052255963", 
              "https://doi.org/10.1038/nrc1367"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm848", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037320126", 
              "https://doi.org/10.1038/nm848"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1202624", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027431068", 
              "https://doi.org/10.1038/sj.onc.1202624"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.bjc.6600875", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033708258", 
              "https://doi.org/10.1038/sj.bjc.6600875"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/labinvest.3780291", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013355064", 
              "https://doi.org/10.1038/labinvest.3780291"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/380439a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004384986", 
              "https://doi.org/10.1038/380439a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1054/bjoc.2001.1971", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017047536", 
              "https://doi.org/10.1054/bjoc.2001.1971"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2010-10-30", 
        "datePublishedReg": "2010-10-30", 
        "description": "BackgroundProstate tumours are commonly poorly oxygenated which is associated with tumour progression and development of resistance to chemotherapeutic drugs and radiotherapy. Fibroblast growth factor 8b (FGF8b) is a mitogenic and angiogenic factor, which is expressed at an increased level in human prostate tumours and is associated with a poor prognosis. We studied the effect of FGF8b on tumour oxygenation and growth parameters in xenografts in comparison with vascular endothelial growth factor (VEGF)-expressing xenografts, representing another fast growing and angiogenic tumour model.MethodsSubcutaneous tumours of PC-3 cells transfected with FGF8b, VEGF or empty (mock) vectors were produced and studied for vascularity, cell proliferation, glucose metabolism and oxygenation. Tumours were evaluated by immunohistochemistry (IHC), flow cytometry, use of radiolabelled markers of energy metabolism ([18F]FDG) and hypoxia ([18F]EF5), and intratumoral polarographic measurements of pO2.ResultsBoth FGF8b and VEGF tumours grew rapidly in nude mice and showed highly vascularised morphology. Perfusion studies, pO2 measurements, [18F]EF5 and [18F]FDG uptake as well as IHC staining for glucose transport protein (GLUT1) and hypoxia inducible factor (HIF) 1 showed that VEGF xenografts were well-perfused and oxygenised, as expected, whereas FGF8b tumours were as hypoxic as mock tumours. These results suggest that FGF8b-induced tumour capillaries are defective. Nevertheless, the growth rate of hypoxic FGF8b tumours was highly increased, as that of well-oxygenised VEGF tumours, when compared with hypoxic mock tumour controls.ConclusionFGF8b is able to induce fast growth in strongly hypoxic tumour microenvironment whereas VEGF-stimulated growth advantage is associated with improved perfusion and oxygenation of prostate tumour xenografts.", 
        "genre": "article", 
        "id": "sg:pub.10.1186/1471-2407-10-596", 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1024632", 
            "issn": [
              "1471-2407"
            ], 
            "name": "BMC Cancer", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "10"
          }
        ], 
        "keywords": [
          "fibroblast growth factor 8b", 
          "prostate tumor xenografts", 
          "VEGF tumors", 
          "tumor xenografts", 
          "PC-3 prostate tumor xenografts", 
          "vascular endothelial growth factor", 
          "human prostate tumors", 
          "endothelial growth factor", 
          "PC-3 cells", 
          "hypoxia-inducible factor-1", 
          "inducible factor-1", 
          "poor prognosis", 
          "improved perfusion", 
          "development of resistance", 
          "tumor control", 
          "nude mice", 
          "prostate tumors", 
          "tumor oxygenation", 
          "radiolabelled marker", 
          "aberrant vasculature", 
          "angiogenic factors", 
          "perfusion studies", 
          "tumor capillaries", 
          "tumor progression", 
          "xenografts", 
          "tumors", 
          "tumor model", 
          "chemotherapeutic drugs", 
          "growth factor", 
          "immunohistochemistry", 
          "cell proliferation", 
          "factor 1", 
          "pO2 measurements", 
          "hypoxic", 
          "oxygenation", 
          "energy metabolism", 
          "empty vector", 
          "hypoxia", 
          "mock tumors", 
          "VEGF", 
          "growth advantage", 
          "glucose transport protein", 
          "metabolism", 
          "prognosis", 
          "radiotherapy", 
          "vascularity", 
          "perfusion", 
          "mice", 
          "progression", 
          "vasculature", 
          "cytometry", 
          "drugs", 
          "factors", 
          "transport proteins", 
          "mitogenic", 
          "proliferation", 
          "markers", 
          "microenvironment", 
          "cells", 
          "uptake", 
          "capillaries", 
          "levels", 
          "control", 
          "protein", 
          "study", 
          "rate", 
          "effect", 
          "resistance", 
          "growth", 
          "use", 
          "development", 
          "comparison", 
          "measurements", 
          "results", 
          "growth rate", 
          "growth parameters", 
          "polarographic measurements", 
          "model", 
          "morphology", 
          "vector", 
          "growing", 
          "fastest growing", 
          "fast growth", 
          "parameters", 
          "advantages"
        ], 
        "name": "Fast growth associated with aberrant vasculature and hypoxia in fibroblast growth factor 8b (FGF8b) over-expressing PC-3 prostate tumour xenografts", 
        "pagination": "596", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1044857053"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/1471-2407-10-596"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "21034500"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/1471-2407-10-596", 
          "https://app.dimensions.ai/details/publication/pub.1044857053"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-10-01T06:36", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_528.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1186/1471-2407-10-596"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-10-596'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-10-596'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-10-596'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2407-10-596'


     

    This table displays all metadata directly associated to this object as RDF triples.

    357 TRIPLES      21 PREDICATES      138 URIs      116 LITERALS      21 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/1471-2407-10-596 schema:about N3735087e8e03478a93a8e66c9f98a328
    2 N4123da3199a543a7981e20fade472073
    3 N4a43e8fe2ca540a6942a1b60843d79c3
    4 N4d42c53d4e954d93996eb1e92857f809
    5 N60d95c3da9ff4f59ba860c0216a3196a
    6 N61c0883d98834e198fc13f230b31fc6a
    7 N7af6917cd10f4e3da86ff204780ea118
    8 N7fcf5317d2cf4c38a48e94a5e4573b56
    9 N8917447ab6b74a06af5a49b1a7038fdd
    10 N9b3cf6e03de047ab9e3d8e15bb137113
    11 Na2019a78149e44519de99348062707dc
    12 Nbb14300863f2484787263cddc2a04acb
    13 Nc3a06a76ca0445bca68c730cefd20d9d
    14 Nfe58cf4c42fb41f0987b21c8b6bd40ac
    15 anzsrc-for:11
    16 anzsrc-for:1112
    17 schema:author Ndeced3716a1a4ead93a0e51e89001682
    18 schema:citation sg:pub.10.1007/s00259-003-1404-x
    19 sg:pub.10.1038/380439a0
    20 sg:pub.10.1038/labinvest.3780291
    21 sg:pub.10.1038/nm848
    22 sg:pub.10.1038/nrc1367
    23 sg:pub.10.1038/nrc1478
    24 sg:pub.10.1038/nrc704
    25 sg:pub.10.1038/sj.bjc.6600875
    26 sg:pub.10.1038/sj.bjc.6604330
    27 sg:pub.10.1038/sj.onc.1201652
    28 sg:pub.10.1038/sj.onc.1202624
    29 sg:pub.10.1038/sj.onc.1204430
    30 sg:pub.10.1054/bjoc.2001.1971
    31 sg:pub.10.1186/1471-2407-8-81
    32 schema:datePublished 2010-10-30
    33 schema:datePublishedReg 2010-10-30
    34 schema:description BackgroundProstate tumours are commonly poorly oxygenated which is associated with tumour progression and development of resistance to chemotherapeutic drugs and radiotherapy. Fibroblast growth factor 8b (FGF8b) is a mitogenic and angiogenic factor, which is expressed at an increased level in human prostate tumours and is associated with a poor prognosis. We studied the effect of FGF8b on tumour oxygenation and growth parameters in xenografts in comparison with vascular endothelial growth factor (VEGF)-expressing xenografts, representing another fast growing and angiogenic tumour model.MethodsSubcutaneous tumours of PC-3 cells transfected with FGF8b, VEGF or empty (mock) vectors were produced and studied for vascularity, cell proliferation, glucose metabolism and oxygenation. Tumours were evaluated by immunohistochemistry (IHC), flow cytometry, use of radiolabelled markers of energy metabolism ([18F]FDG) and hypoxia ([18F]EF5), and intratumoral polarographic measurements of pO2.ResultsBoth FGF8b and VEGF tumours grew rapidly in nude mice and showed highly vascularised morphology. Perfusion studies, pO2 measurements, [18F]EF5 and [18F]FDG uptake as well as IHC staining for glucose transport protein (GLUT1) and hypoxia inducible factor (HIF) 1 showed that VEGF xenografts were well-perfused and oxygenised, as expected, whereas FGF8b tumours were as hypoxic as mock tumours. These results suggest that FGF8b-induced tumour capillaries are defective. Nevertheless, the growth rate of hypoxic FGF8b tumours was highly increased, as that of well-oxygenised VEGF tumours, when compared with hypoxic mock tumour controls.ConclusionFGF8b is able to induce fast growth in strongly hypoxic tumour microenvironment whereas VEGF-stimulated growth advantage is associated with improved perfusion and oxygenation of prostate tumour xenografts.
    35 schema:genre article
    36 schema:isAccessibleForFree true
    37 schema:isPartOf N423d314d92414703ba202e4624890dbb
    38 N6837cf13424f479a9acce2e48ad80b85
    39 sg:journal.1024632
    40 schema:keywords PC-3 cells
    41 PC-3 prostate tumor xenografts
    42 VEGF
    43 VEGF tumors
    44 aberrant vasculature
    45 advantages
    46 angiogenic factors
    47 capillaries
    48 cell proliferation
    49 cells
    50 chemotherapeutic drugs
    51 comparison
    52 control
    53 cytometry
    54 development
    55 development of resistance
    56 drugs
    57 effect
    58 empty vector
    59 endothelial growth factor
    60 energy metabolism
    61 factor 1
    62 factors
    63 fast growth
    64 fastest growing
    65 fibroblast growth factor 8b
    66 glucose transport protein
    67 growing
    68 growth
    69 growth advantage
    70 growth factor
    71 growth parameters
    72 growth rate
    73 human prostate tumors
    74 hypoxia
    75 hypoxia-inducible factor-1
    76 hypoxic
    77 immunohistochemistry
    78 improved perfusion
    79 inducible factor-1
    80 levels
    81 markers
    82 measurements
    83 metabolism
    84 mice
    85 microenvironment
    86 mitogenic
    87 mock tumors
    88 model
    89 morphology
    90 nude mice
    91 oxygenation
    92 pO2 measurements
    93 parameters
    94 perfusion
    95 perfusion studies
    96 polarographic measurements
    97 poor prognosis
    98 prognosis
    99 progression
    100 proliferation
    101 prostate tumor xenografts
    102 prostate tumors
    103 protein
    104 radiolabelled marker
    105 radiotherapy
    106 rate
    107 resistance
    108 results
    109 study
    110 transport proteins
    111 tumor capillaries
    112 tumor control
    113 tumor model
    114 tumor oxygenation
    115 tumor progression
    116 tumor xenografts
    117 tumors
    118 uptake
    119 use
    120 vascular endothelial growth factor
    121 vascularity
    122 vasculature
    123 vector
    124 xenografts
    125 schema:name Fast growth associated with aberrant vasculature and hypoxia in fibroblast growth factor 8b (FGF8b) over-expressing PC-3 prostate tumour xenografts
    126 schema:pagination 596
    127 schema:productId N1820d6d92355458ca8f35a2e2cbf9e76
    128 N1907b53e91c149fb989f80a3cf437f74
    129 N4281fb2be7104972a3045a3f80541a5e
    130 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044857053
    131 https://doi.org/10.1186/1471-2407-10-596
    132 schema:sdDatePublished 2022-10-01T06:36
    133 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    134 schema:sdPublisher N895f159688884fddada239d00c858485
    135 schema:url https://doi.org/10.1186/1471-2407-10-596
    136 sgo:license sg:explorer/license/
    137 sgo:sdDataset articles
    138 rdf:type schema:ScholarlyArticle
    139 N0d1e96d2a3dc4aa9b296735207c5dbca rdf:first sg:person.01050347723.38
    140 rdf:rest N0e05f7cbd8134fe596cb6b78ed8c4e26
    141 N0e05f7cbd8134fe596cb6b78ed8c4e26 rdf:first sg:person.01057277261.68
    142 rdf:rest Na708484577ad4ce08154dce110eecaee
    143 N1553c1b0aa504763a772c3bb5f23fcfc rdf:first sg:person.01335344475.48
    144 rdf:rest N9716f90ac24948c2bf072443b4c47c45
    145 N1820d6d92355458ca8f35a2e2cbf9e76 schema:name pubmed_id
    146 schema:value 21034500
    147 rdf:type schema:PropertyValue
    148 N1907b53e91c149fb989f80a3cf437f74 schema:name dimensions_id
    149 schema:value pub.1044857053
    150 rdf:type schema:PropertyValue
    151 N3735087e8e03478a93a8e66c9f98a328 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name Animals
    153 rdf:type schema:DefinedTerm
    154 N4123da3199a543a7981e20fade472073 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    155 schema:name Humans
    156 rdf:type schema:DefinedTerm
    157 N423d314d92414703ba202e4624890dbb schema:issueNumber 1
    158 rdf:type schema:PublicationIssue
    159 N4281fb2be7104972a3045a3f80541a5e schema:name doi
    160 schema:value 10.1186/1471-2407-10-596
    161 rdf:type schema:PropertyValue
    162 N4a43e8fe2ca540a6942a1b60843d79c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    163 schema:name Mice, Nude
    164 rdf:type schema:DefinedTerm
    165 N4d42c53d4e954d93996eb1e92857f809 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    166 schema:name Oxygen
    167 rdf:type schema:DefinedTerm
    168 N60d95c3da9ff4f59ba860c0216a3196a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    169 schema:name Prostatic Neoplasms
    170 rdf:type schema:DefinedTerm
    171 N61c0883d98834e198fc13f230b31fc6a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    172 schema:name Male
    173 rdf:type schema:DefinedTerm
    174 N6837cf13424f479a9acce2e48ad80b85 schema:volumeNumber 10
    175 rdf:type schema:PublicationVolume
    176 N7af6917cd10f4e3da86ff204780ea118 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name Fibroblast Growth Factor 8
    178 rdf:type schema:DefinedTerm
    179 N7fcf5317d2cf4c38a48e94a5e4573b56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    180 schema:name Vascular Endothelial Growth Factor A
    181 rdf:type schema:DefinedTerm
    182 N8917447ab6b74a06af5a49b1a7038fdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    183 schema:name Hypoxia
    184 rdf:type schema:DefinedTerm
    185 N895f159688884fddada239d00c858485 schema:name Springer Nature - SN SciGraph project
    186 rdf:type schema:Organization
    187 N8b81a8b894604c30bbc70d1a411f7cd2 rdf:first sg:person.0722714143.14
    188 rdf:rest Ne7377e63e312452bb224f91029b1dacb
    189 N9716f90ac24948c2bf072443b4c47c45 rdf:first sg:person.0656631675.01
    190 rdf:rest Nb9c7e59871514f58bc4e26628ac94099
    191 N9b3cf6e03de047ab9e3d8e15bb137113 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    192 schema:name Mice
    193 rdf:type schema:DefinedTerm
    194 Na0f114082a404d998e03b1cccb685020 rdf:first sg:person.01131663575.79
    195 rdf:rest rdf:nil
    196 Na2019a78149e44519de99348062707dc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    197 schema:name Prognosis
    198 rdf:type schema:DefinedTerm
    199 Na59c6d8ff18046f3b0727c7b5f5e012d rdf:first sg:person.01176116005.37
    200 rdf:rest N8b81a8b894604c30bbc70d1a411f7cd2
    201 Na708484577ad4ce08154dce110eecaee rdf:first sg:person.01071362053.45
    202 rdf:rest N1553c1b0aa504763a772c3bb5f23fcfc
    203 Nb9c7e59871514f58bc4e26628ac94099 rdf:first sg:person.0700413711.55
    204 rdf:rest Na0f114082a404d998e03b1cccb685020
    205 Nbb14300863f2484787263cddc2a04acb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    206 schema:name Transfection
    207 rdf:type schema:DefinedTerm
    208 Nc3a06a76ca0445bca68c730cefd20d9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    209 schema:name Gene Expression Regulation, Neoplastic
    210 rdf:type schema:DefinedTerm
    211 Ndeced3716a1a4ead93a0e51e89001682 rdf:first sg:person.01244231205.07
    212 rdf:rest Nf0fb10655f7c4a159ee8bf78abd60291
    213 Ne7377e63e312452bb224f91029b1dacb rdf:first sg:person.01221116075.20
    214 rdf:rest N0d1e96d2a3dc4aa9b296735207c5dbca
    215 Nf0fb10655f7c4a159ee8bf78abd60291 rdf:first sg:person.01102756462.43
    216 rdf:rest Na59c6d8ff18046f3b0727c7b5f5e012d
    217 Nfe58cf4c42fb41f0987b21c8b6bd40ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    218 schema:name Neoplasm Transplantation
    219 rdf:type schema:DefinedTerm
    220 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    221 schema:name Medical and Health Sciences
    222 rdf:type schema:DefinedTerm
    223 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
    224 schema:name Oncology and Carcinogenesis
    225 rdf:type schema:DefinedTerm
    226 sg:journal.1024632 schema:issn 1471-2407
    227 schema:name BMC Cancer
    228 schema:publisher Springer Nature
    229 rdf:type schema:Periodical
    230 sg:person.01050347723.38 schema:affiliation grid-institutes:grid.452714.1
    231 schema:familyName Seppänen
    232 schema:givenName Jani
    233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050347723.38
    234 rdf:type schema:Person
    235 sg:person.01057277261.68 schema:affiliation grid-institutes:grid.470895.7
    236 schema:familyName Marjamäki
    237 schema:givenName Päivi
    238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057277261.68
    239 rdf:type schema:Person
    240 sg:person.01071362053.45 schema:affiliation grid-institutes:grid.470895.7
    241 schema:familyName Forsback
    242 schema:givenName Sarita
    243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071362053.45
    244 rdf:type schema:Person
    245 sg:person.01102756462.43 schema:affiliation grid-institutes:grid.470895.7
    246 schema:familyName Grönroos
    247 schema:givenName Tove J
    248 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102756462.43
    249 rdf:type schema:Person
    250 sg:person.01131663575.79 schema:affiliation grid-institutes:grid.4514.4
    251 schema:familyName Härkönen
    252 schema:givenName Pirkko L
    253 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131663575.79
    254 rdf:type schema:Person
    255 sg:person.01176116005.37 schema:affiliation grid-institutes:grid.1374.1
    256 schema:familyName Valta
    257 schema:givenName Maija P
    258 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01176116005.37
    259 rdf:type schema:Person
    260 sg:person.01221116075.20 schema:affiliation grid-institutes:grid.1374.1
    261 schema:familyName Schrey
    262 schema:givenName Aleksi
    263 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221116075.20
    264 rdf:type schema:Person
    265 sg:person.01244231205.07 schema:affiliation grid-institutes:grid.452714.1
    266 schema:familyName Tuomela
    267 schema:givenName Johanna
    268 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244231205.07
    269 rdf:type schema:Person
    270 sg:person.01335344475.48 schema:affiliation grid-institutes:grid.1374.1
    271 schema:familyName Kinnunen
    272 schema:givenName Ilpo
    273 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335344475.48
    274 rdf:type schema:Person
    275 sg:person.0656631675.01 schema:affiliation grid-institutes:grid.470895.7
    276 schema:familyName Solin
    277 schema:givenName Olof
    278 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656631675.01
    279 rdf:type schema:Person
    280 sg:person.0700413711.55 schema:affiliation grid-institutes:grid.410552.7
    281 schema:familyName Minn
    282 schema:givenName Heikki
    283 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700413711.55
    284 rdf:type schema:Person
    285 sg:person.0722714143.14 schema:affiliation grid-institutes:grid.452861.c
    286 schema:familyName Sandholm
    287 schema:givenName Jouko
    288 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722714143.14
    289 rdf:type schema:Person
    290 sg:pub.10.1007/s00259-003-1404-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019896114
    291 https://doi.org/10.1007/s00259-003-1404-x
    292 rdf:type schema:CreativeWork
    293 sg:pub.10.1038/380439a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004384986
    294 https://doi.org/10.1038/380439a0
    295 rdf:type schema:CreativeWork
    296 sg:pub.10.1038/labinvest.3780291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013355064
    297 https://doi.org/10.1038/labinvest.3780291
    298 rdf:type schema:CreativeWork
    299 sg:pub.10.1038/nm848 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037320126
    300 https://doi.org/10.1038/nm848
    301 rdf:type schema:CreativeWork
    302 sg:pub.10.1038/nrc1367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052255963
    303 https://doi.org/10.1038/nrc1367
    304 rdf:type schema:CreativeWork
    305 sg:pub.10.1038/nrc1478 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032257073
    306 https://doi.org/10.1038/nrc1478
    307 rdf:type schema:CreativeWork
    308 sg:pub.10.1038/nrc704 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028740665
    309 https://doi.org/10.1038/nrc704
    310 rdf:type schema:CreativeWork
    311 sg:pub.10.1038/sj.bjc.6600875 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033708258
    312 https://doi.org/10.1038/sj.bjc.6600875
    313 rdf:type schema:CreativeWork
    314 sg:pub.10.1038/sj.bjc.6604330 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031788073
    315 https://doi.org/10.1038/sj.bjc.6604330
    316 rdf:type schema:CreativeWork
    317 sg:pub.10.1038/sj.onc.1201652 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025156232
    318 https://doi.org/10.1038/sj.onc.1201652
    319 rdf:type schema:CreativeWork
    320 sg:pub.10.1038/sj.onc.1202624 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027431068
    321 https://doi.org/10.1038/sj.onc.1202624
    322 rdf:type schema:CreativeWork
    323 sg:pub.10.1038/sj.onc.1204430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024680700
    324 https://doi.org/10.1038/sj.onc.1204430
    325 rdf:type schema:CreativeWork
    326 sg:pub.10.1054/bjoc.2001.1971 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017047536
    327 https://doi.org/10.1054/bjoc.2001.1971
    328 rdf:type schema:CreativeWork
    329 sg:pub.10.1186/1471-2407-8-81 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009684555
    330 https://doi.org/10.1186/1471-2407-8-81
    331 rdf:type schema:CreativeWork
    332 grid-institutes:grid.1374.1 schema:alternateName Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland
    333 Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, Turku, Finland
    334 schema:name Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland
    335 Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, Turku, Finland
    336 rdf:type schema:Organization
    337 grid-institutes:grid.410552.7 schema:alternateName Department of Oncology and Radiotherapy, Turku University Hospital, Turku, Finland
    338 schema:name Department of Oncology and Radiotherapy, Turku University Hospital, Turku, Finland
    339 rdf:type schema:Organization
    340 grid-institutes:grid.4514.4 schema:alternateName Department of Laboratory Medicine, MAS University Hospital, Lund University, Malmö, Sweden
    341 schema:name Department of Laboratory Medicine, MAS University Hospital, Lund University, Malmö, Sweden
    342 Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, Turku, Finland
    343 rdf:type schema:Organization
    344 grid-institutes:grid.452714.1 schema:alternateName Pharmatest Services Ltd., Turku, Finland
    345 schema:name Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, Turku, Finland
    346 Pharmatest Services Ltd., Turku, Finland
    347 rdf:type schema:Organization
    348 grid-institutes:grid.452861.c schema:alternateName Cell Imaging Core, Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland
    349 schema:name Cell Imaging Core, Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland
    350 rdf:type schema:Organization
    351 grid-institutes:grid.470895.7 schema:alternateName Turku PET Centre, Accelerator Laboratory, MediCity Preclinical Research Laboratory, University of Turku and Åbo Akademi University, Turku, Finland
    352 Turku PET Centre, MediCity Preclinical Research Laboratory, University of Turku and Åbo Akademi University, Turku, Finland
    353 Turku PET Centre, Radiopharmaceutical Chemistry Laboratory, University of Turku and Åbo Akademi University, Turku, Finland
    354 schema:name Turku PET Centre, Accelerator Laboratory, MediCity Preclinical Research Laboratory, University of Turku and Åbo Akademi University, Turku, Finland
    355 Turku PET Centre, MediCity Preclinical Research Laboratory, University of Turku and Åbo Akademi University, Turku, Finland
    356 Turku PET Centre, Radiopharmaceutical Chemistry Laboratory, University of Turku and Åbo Akademi University, Turku, Finland
    357 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...