Ontology type: schema:ScholarlyArticle Open Access: True
2009-12
AUTHORSNoha G Khalaf, Mona M Eletreby, Nancy D Hanson
ABSTRACTBACKGROUND: A high rate of resistance to 3rd generation cephalosporins among Enterobacteriaceae isolates from Egypt has been previously reported. This study aims to characterize the resistance mechanism (s) to extended spectrum cephalosporins among resistant clinical isolates at a medical institute in Cairo, Egypt. METHODS: Nonconsecutive Klebsiella pneumoniae (Kp), Enterobacter cloacae (ENT) and Escherichia coli (EC) isolates were obtained from the clinical laboratory at the medical institute. Antibiotic susceptibility was tested by CLSI disk diffusion and ESBL confirmatory tests. MICs were determined using broth microdilution. Isoelectric focusing (IEF) was used to determine the pI values, inhibitor profiles, and cefotaxime (CTX) hydrolysis by the beta-lactamases. PCR and sequencing were performed using blaCTX-M and ISEcp1-specific primers, with DNA obtained from the clinical isolates. Conjugation experiments were done to determine the mobility of blaCTX-M. RESULTS: All five clinical isolates were resistant to CTX, and were positive for ESBL screening. IEF revealed multiple beta-lactamases produced by each isolate, including a beta-lactamase with a pI of 8.0 in Kp and ENT and a beta-lactamase with a pI of 9.0 in EC. Both beta-lactamases were inhibited by clavulanic acid and hydrolyzed CTX. PCR and sequence analysis identified blaCTX-M-14 in Kp and ENT and a blaCTX-M-15 in EC. Both blaCTX-M-14 and blaCTX-M-15 were preceded by ISEcp1 elements as revealed by partial sequence analysis of the upstream region of the blaCTX-M genes. blaCTX-M-15 was transferable but not blaCTX-M-14. CONCLUSION: This is the first report of CTX-M-14 in Kp and ENT isolates from Egypt, the Middle East and North Africa. More... »
PAGES84
http://scigraph.springernature.com/pub.10.1186/1471-2334-9-84
DOIhttp://dx.doi.org/10.1186/1471-2334-9-84
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1038311916
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/19497111
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical Microbiology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Anti-Bacterial Agents",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Conjugation, Genetic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Drug Resistance, Multiple, Bacterial",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Egypt",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Enterobacter cloacae",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Enterobacteriaceae Infections",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Escherichia coli",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genes, Bacterial",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Isoelectric Focusing",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Klebsiella pneumoniae",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Microbial Sensitivity Tests",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "beta-Lactamases",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Helwan University",
"id": "https://www.grid.ac/institutes/grid.412093.d",
"name": [
"Department of Microbiology and Immunology, Faculty of Pharmacy Helwan University, Helwan, Egypt"
],
"type": "Organization"
},
"familyName": "Khalaf",
"givenName": "Noha G",
"id": "sg:person.01034643070.09",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034643070.09"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Heart Institute",
"id": "https://www.grid.ac/institutes/grid.489068.b",
"name": [
"Clinical Microbiology department, National Heart Institute, Cairo, Egypt"
],
"type": "Organization"
},
"familyName": "Eletreby",
"givenName": "Mona M",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Creighton University",
"id": "https://www.grid.ac/institutes/grid.254748.8",
"name": [
"Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska, USA"
],
"type": "Organization"
},
"familyName": "Hanson",
"givenName": "Nancy D",
"id": "sg:person.01370453651.63",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370453651.63"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1128/jcm.42.12.5715-5721.2004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002730527"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3201/eid0805.010346",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005562237"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/cmr.18.4.657-686.2005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007739875"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jcm.43.7.3309-3313.2005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016153268"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.ijantimicag.2004.01.010",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016264261"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aac.48.1.1-14.2004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016807294"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/jac/dkm316",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022693472"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aac.47.7.2382-2383.2003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026664264"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/jac/dkg101",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030851991"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aac.30.6.951",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031398658"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.ijantimicag.2006.01.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032737229"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/jac/44.3.377",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033003366"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aac.46.9.3045-3049.2002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033656859"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jcm.43.3.1037-1044.2005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034193244"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aac.45.12.3355-3361.2001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036691750"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aac.49.3.1150-1156.2005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042094154"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.inf.0000168804.09875.95",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042146071"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.inf.0000168804.09875.95",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042146071"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.inf.0000168804.09875.95",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042146071"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aac.47.9.2938-2945.2003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043464301"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/jac/dkf240",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044690916"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.ijantimicag.2005.12.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048629404"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aac.46.12.4038-4040.2002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062709934"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2807/esm.11.07.00639-en",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1077295158"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aac.42.3.596",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083233679"
],
"type": "CreativeWork"
}
],
"datePublished": "2009-12",
"datePublishedReg": "2009-12-01",
"description": "BACKGROUND: A high rate of resistance to 3rd generation cephalosporins among Enterobacteriaceae isolates from Egypt has been previously reported. This study aims to characterize the resistance mechanism (s) to extended spectrum cephalosporins among resistant clinical isolates at a medical institute in Cairo, Egypt.\nMETHODS: Nonconsecutive Klebsiella pneumoniae (Kp), Enterobacter cloacae (ENT) and Escherichia coli (EC) isolates were obtained from the clinical laboratory at the medical institute. Antibiotic susceptibility was tested by CLSI disk diffusion and ESBL confirmatory tests. MICs were determined using broth microdilution. Isoelectric focusing (IEF) was used to determine the pI values, inhibitor profiles, and cefotaxime (CTX) hydrolysis by the beta-lactamases. PCR and sequencing were performed using blaCTX-M and ISEcp1-specific primers, with DNA obtained from the clinical isolates. Conjugation experiments were done to determine the mobility of blaCTX-M.\nRESULTS: All five clinical isolates were resistant to CTX, and were positive for ESBL screening. IEF revealed multiple beta-lactamases produced by each isolate, including a beta-lactamase with a pI of 8.0 in Kp and ENT and a beta-lactamase with a pI of 9.0 in EC. Both beta-lactamases were inhibited by clavulanic acid and hydrolyzed CTX. PCR and sequence analysis identified blaCTX-M-14 in Kp and ENT and a blaCTX-M-15 in EC. Both blaCTX-M-14 and blaCTX-M-15 were preceded by ISEcp1 elements as revealed by partial sequence analysis of the upstream region of the blaCTX-M genes. blaCTX-M-15 was transferable but not blaCTX-M-14.\nCONCLUSION: This is the first report of CTX-M-14 in Kp and ENT isolates from Egypt, the Middle East and North Africa.",
"genre": "research_article",
"id": "sg:pub.10.1186/1471-2334-9-84",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1024946",
"issn": [
"1471-2334"
],
"name": "BMC Infectious Diseases",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "9"
}
],
"name": "Characterization of CTX-M ESBLs in Enterobacter cloacae, Escherichia coli and Klebsiella pneumoniae clinical isolates from Cairo, Egypt",
"pagination": "84",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"b680efe4e6480cabecd39b85aa9ae9a184dace25af39365a6dec87424a784dfc"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"19497111"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"100968551"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1186/1471-2334-9-84"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1038311916"
]
}
],
"sameAs": [
"https://doi.org/10.1186/1471-2334-9-84",
"https://app.dimensions.ai/details/publication/pub.1038311916"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T01:58",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000506.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1186%2F1471-2334-9-84"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2334-9-84'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2334-9-84'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2334-9-84'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2334-9-84'
This table displays all metadata directly associated to this object as RDF triples.
208 TRIPLES
21 PREDICATES
65 URIs
34 LITERALS
22 BLANK NODES