Molecular detection of fluoroquinolone-resistance in multi-drug resistant tuberculosis in Cambodia suggests low association with XDR phenotypes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-12

AUTHORS

Corinne Surcouf, Seiha Heng, Catherine Pierre-Audigier, Véronique Cadet-Daniel, Amine Namouchi, Alan Murray, Brigitte Gicquel, Bertrand Guillard

ABSTRACT

BACKGROUND: Drug susceptibility testing (DST) remains an important concern for implementing treatment of MDR tuberculosis patients. Implementation of molecular tests for drug resistance identification would facilitate DST particularly in developing countries where culturing is difficult to perform. We have characterized multidrug resistant strains in Cambodia using MDTDRsl tests, drug target sequencing and phenotypic tests. METHODS: A total of 65 non-MDR and 101 MDR TB isolates collected between May 2007 and June 2009 were tested for resistance to fluoroquinolones and aminoglycosides/cyclic peptides using the GenoType® MTBDRsl assay and gene sequencing. Rifampicin resistance (RMP-R) was tested using gene sequencing and genotyping was assessed by spoligotyping. RESULTS: A total of 95 of the 101 MDR strains were confirmed to be RMP-R by rpoB gene sequencing. Fourteen of the 101 MDR isolates (14%) carried a gyrA mutation associated with fluoroquinolone-resistance (FQ-R) (detected by the MTBDRsl assay and sequencing) compared with only 1 (1.5%) of the 65 non-MDR strains. Only 1 (1%) of the MDR isolates was found to be XDR TB. The MDR group contained a higher proportion of Beijing or Beijing like strains (58%) than the non MDR group (28%). This percentage is higher in MDR FQ-R strains (71%). CONCLUSIONS: The new GenoType® MTBDRsl assay combined with molecular tests to detect RMP-R and isoniazid resistance (INH-R) represents a valuable tool for the detection of XDR TB. In Cambodia there is a low rate of XDR amongst MDR TB including MDR FQ-R TB. This suggests a low association between FQ-R and XDR TB. Strain spoligotyping confirms Beijing strains to be more prone to accumulate antibiotic resistance. More... »

PAGES

255

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2334-11-255

DOI

http://dx.doi.org/10.1186/1471-2334-11-255

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017063275

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21955640


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antitubercular Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cambodia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Gyrase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Directed RNA Polymerases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluoroquinolones", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microbial Sensitivity Tests", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mycobacterium tuberculosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tuberculosis, Multidrug-Resistant", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institut Pasteur du Cambodge", 
          "id": "https://www.grid.ac/institutes/grid.418537.c", 
          "name": [
            "Mycobacteriology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Surcouf", 
        "givenName": "Corinne", 
        "id": "sg:person.0613560627.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613560627.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur du Cambodge", 
          "id": "https://www.grid.ac/institutes/grid.418537.c", 
          "name": [
            "Mycobacteriology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Heng", 
        "givenName": "Seiha", 
        "id": "sg:person.01250162317.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250162317.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur", 
          "id": "https://www.grid.ac/institutes/grid.428999.7", 
          "name": [
            "Unit\u00e9 de G\u00e9n\u00e9tique Mycobact\u00e9rienne, Institut Pasteur, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pierre-Audigier", 
        "givenName": "Catherine", 
        "id": "sg:person.0777352714.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777352714.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur", 
          "id": "https://www.grid.ac/institutes/grid.428999.7", 
          "name": [
            "Unit\u00e9 de G\u00e9n\u00e9tique Mycobact\u00e9rienne, Institut Pasteur, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cadet-Daniel", 
        "givenName": "V\u00e9ronique", 
        "id": "sg:person.01022302627.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022302627.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur", 
          "id": "https://www.grid.ac/institutes/grid.428999.7", 
          "name": [
            "Unit\u00e9 de G\u00e9n\u00e9tique Mycobact\u00e9rienne, Institut Pasteur, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Namouchi", 
        "givenName": "Amine", 
        "id": "sg:person.0743525275.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743525275.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur", 
          "id": "https://www.grid.ac/institutes/grid.428999.7", 
          "name": [
            "Unit\u00e9 de G\u00e9n\u00e9tique Mycobact\u00e9rienne, Institut Pasteur, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murray", 
        "givenName": "Alan", 
        "id": "sg:person.013107235601.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013107235601.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur", 
          "id": "https://www.grid.ac/institutes/grid.428999.7", 
          "name": [
            "Unit\u00e9 de G\u00e9n\u00e9tique Mycobact\u00e9rienne, Institut Pasteur, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gicquel", 
        "givenName": "Brigitte", 
        "id": "sg:person.01202402243.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202402243.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur du Cambodge", 
          "id": "https://www.grid.ac/institutes/grid.418537.c", 
          "name": [
            "Mycobacteriology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guillard", 
        "givenName": "Bertrand", 
        "id": "sg:person.01130643176.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130643176.86"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1086/505877", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001797747"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.46.5.1417-1424.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002327339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.00177-09", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006496592"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.2165/00003495-200767140-00007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012071676", 
          "https://doi.org/10.2165/00003495-200767140-00007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkm061", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014376523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10096-003-0944-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017980690", 
          "https://doi.org/10.1007/s10096-003-0944-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dki353", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020240436"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1473-3099(07)70141-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023650388"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/infdis/174.5.1127", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023740802"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm199302253280802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026900723"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1469-0691.2009.03078.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027933747"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.00081-09", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028959980"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.00750-06", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032497579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0923-2508(02)01311-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032843541"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(09)60333-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035006035"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.18.9869", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035792044"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/427292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035959901"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/427292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035959901"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.00201-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042156203"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1405.071398", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043882428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/147323000903700517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044272108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/147323000903700517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044272108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm199510053331404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051126112"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10096-004-1268-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051992616", 
          "https://doi.org/10.1007/s10096-004-1268-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074595044", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074836077", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077270486", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077523365", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077599504", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077835620", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077878614", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078164728", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.40.8.1768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082972998"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083091586", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-12", 
    "datePublishedReg": "2011-12-01", 
    "description": "BACKGROUND: Drug susceptibility testing (DST) remains an important concern for implementing treatment of MDR tuberculosis patients. Implementation of molecular tests for drug resistance identification would facilitate DST particularly in developing countries where culturing is difficult to perform. We have characterized multidrug resistant strains in Cambodia using MDTDRsl tests, drug target sequencing and phenotypic tests.\nMETHODS: A total of 65 non-MDR and 101 MDR TB isolates collected between May 2007 and June 2009 were tested for resistance to fluoroquinolones and aminoglycosides/cyclic peptides using the GenoType\u00ae MTBDRsl assay and gene sequencing. Rifampicin resistance (RMP-R) was tested using gene sequencing and genotyping was assessed by spoligotyping.\nRESULTS: A total of 95 of the 101 MDR strains were confirmed to be RMP-R by rpoB gene sequencing. Fourteen of the 101 MDR isolates (14%) carried a gyrA mutation associated with fluoroquinolone-resistance (FQ-R) (detected by the MTBDRsl assay and sequencing) compared with only 1 (1.5%) of the 65 non-MDR strains. Only 1 (1%) of the MDR isolates was found to be XDR TB. The MDR group contained a higher proportion of Beijing or Beijing like strains (58%) than the non MDR group (28%). This percentage is higher in MDR FQ-R strains (71%).\nCONCLUSIONS: The new GenoType\u00ae MTBDRsl assay combined with molecular tests to detect RMP-R and isoniazid resistance (INH-R) represents a valuable tool for the detection of XDR TB. In Cambodia there is a low rate of XDR amongst MDR TB including MDR FQ-R TB. This suggests a low association between FQ-R and XDR TB. Strain spoligotyping confirms Beijing strains to be more prone to accumulate antibiotic resistance.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1471-2334-11-255", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3776207", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1024946", 
        "issn": [
          "1471-2334"
        ], 
        "name": "BMC Infectious Diseases", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "11"
      }
    ], 
    "name": "Molecular detection of fluoroquinolone-resistance in multi-drug resistant tuberculosis in Cambodia suggests low association with XDR phenotypes", 
    "pagination": "255", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "514be517c98129b25f572664695ed0895f97e31959c3950fc5a8b15f37492220"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21955640"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100968551"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2334-11-255"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1017063275"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2334-11-255", 
      "https://app.dimensions.ai/details/publication/pub.1017063275"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T22:39", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000549.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1471-2334-11-255"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2334-11-255'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2334-11-255'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2334-11-255'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2334-11-255'


 

This table displays all metadata directly associated to this object as RDF triples.

268 TRIPLES      21 PREDICATES      75 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2334-11-255 schema:about N1b02b7d3d78843e59de8e3d52312697e
2 N1b1e3449f41c43cb9ed2ed14bae1fc60
3 N325374bc90ab4ee592a1eb67c4837494
4 N581f365d6bbb4bb68096de136d1f1d06
5 N8b631c9bcbbc45af909fccb7cd1aa38e
6 N974a7df2ae024e20a8dbd274ddb87ac5
7 N980898bce68c4eab9661a73871bf4840
8 Nad22f0b5c47141f986dc5408a79bd12d
9 Nb1ec39ca839f4cedb1e114b317b58164
10 Nb97710297b3945f28ee76be38a67fd1e
11 Nf017331a79c5492380b8e34b802942bf
12 Nf1b76f35f47d4e5c923ac6c75756b844
13 Nf591db2722db41f2beb71ba0d494a891
14 Nfa787d2376e549bbbfb2f5841ff99d26
15 anzsrc-for:11
16 anzsrc-for:1108
17 schema:author Ndc446d7e804a4fdb9c3c15f7d5d7160c
18 schema:citation sg:pub.10.1007/s10096-003-0944-0
19 sg:pub.10.1007/s10096-004-1268-4
20 sg:pub.10.2165/00003495-200767140-00007
21 https://app.dimensions.ai/details/publication/pub.1074595044
22 https://app.dimensions.ai/details/publication/pub.1074836077
23 https://app.dimensions.ai/details/publication/pub.1077270486
24 https://app.dimensions.ai/details/publication/pub.1077523365
25 https://app.dimensions.ai/details/publication/pub.1077599504
26 https://app.dimensions.ai/details/publication/pub.1077835620
27 https://app.dimensions.ai/details/publication/pub.1077878614
28 https://app.dimensions.ai/details/publication/pub.1078164728
29 https://app.dimensions.ai/details/publication/pub.1083091586
30 https://doi.org/10.1016/s0140-6736(09)60333-0
31 https://doi.org/10.1016/s0923-2508(02)01311-6
32 https://doi.org/10.1016/s1473-3099(07)70141-x
33 https://doi.org/10.1056/nejm199302253280802
34 https://doi.org/10.1056/nejm199510053331404
35 https://doi.org/10.1073/pnas.94.18.9869
36 https://doi.org/10.1086/427292
37 https://doi.org/10.1086/505877
38 https://doi.org/10.1093/infdis/174.5.1127
39 https://doi.org/10.1093/jac/dki353
40 https://doi.org/10.1093/jac/dkm061
41 https://doi.org/10.1111/j.1469-0691.2009.03078.x
42 https://doi.org/10.1128/aac.00177-09
43 https://doi.org/10.1128/aac.40.8.1768
44 https://doi.org/10.1128/aac.46.5.1417-1424.2002
45 https://doi.org/10.1128/jcm.00081-09
46 https://doi.org/10.1128/jcm.00201-10
47 https://doi.org/10.1128/jcm.00750-06
48 https://doi.org/10.1177/147323000903700517
49 https://doi.org/10.3201/eid1405.071398
50 schema:datePublished 2011-12
51 schema:datePublishedReg 2011-12-01
52 schema:description BACKGROUND: Drug susceptibility testing (DST) remains an important concern for implementing treatment of MDR tuberculosis patients. Implementation of molecular tests for drug resistance identification would facilitate DST particularly in developing countries where culturing is difficult to perform. We have characterized multidrug resistant strains in Cambodia using MDTDRsl tests, drug target sequencing and phenotypic tests. METHODS: A total of 65 non-MDR and 101 MDR TB isolates collected between May 2007 and June 2009 were tested for resistance to fluoroquinolones and aminoglycosides/cyclic peptides using the GenoType® MTBDRsl assay and gene sequencing. Rifampicin resistance (RMP-R) was tested using gene sequencing and genotyping was assessed by spoligotyping. RESULTS: A total of 95 of the 101 MDR strains were confirmed to be RMP-R by rpoB gene sequencing. Fourteen of the 101 MDR isolates (14%) carried a gyrA mutation associated with fluoroquinolone-resistance (FQ-R) (detected by the MTBDRsl assay and sequencing) compared with only 1 (1.5%) of the 65 non-MDR strains. Only 1 (1%) of the MDR isolates was found to be XDR TB. The MDR group contained a higher proportion of Beijing or Beijing like strains (58%) than the non MDR group (28%). This percentage is higher in MDR FQ-R strains (71%). CONCLUSIONS: The new GenoType® MTBDRsl assay combined with molecular tests to detect RMP-R and isoniazid resistance (INH-R) represents a valuable tool for the detection of XDR TB. In Cambodia there is a low rate of XDR amongst MDR TB including MDR FQ-R TB. This suggests a low association between FQ-R and XDR TB. Strain spoligotyping confirms Beijing strains to be more prone to accumulate antibiotic resistance.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree true
56 schema:isPartOf N2ac9c08ac790439d9a57e151e4111754
57 N41ea1232c07d428c9260f6a7f607560b
58 sg:journal.1024946
59 schema:name Molecular detection of fluoroquinolone-resistance in multi-drug resistant tuberculosis in Cambodia suggests low association with XDR phenotypes
60 schema:pagination 255
61 schema:productId N3fe50f14757d4a10bfc2ef9b4dca8a8f
62 N4a756bc366ee4663a3852228d08d5588
63 N7058f954f135479eb40e3160084077a2
64 Nd54ab475ee87400bacebc21c05082079
65 Neac2d4b658d142d597d400e114b2ea9f
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017063275
67 https://doi.org/10.1186/1471-2334-11-255
68 schema:sdDatePublished 2019-04-10T22:39
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher N38399da64695487197472191745e12f1
71 schema:url http://link.springer.com/10.1186%2F1471-2334-11-255
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N15db1d7bba644634ad65f21f94bb1046 rdf:first sg:person.0743525275.80
76 rdf:rest N9322d9b441234fdca855611220443c02
77 N1931bbdf05714b1387e9d58c58513b6b rdf:first sg:person.01130643176.86
78 rdf:rest rdf:nil
79 N1b02b7d3d78843e59de8e3d52312697e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name DNA Gyrase
81 rdf:type schema:DefinedTerm
82 N1b1e3449f41c43cb9ed2ed14bae1fc60 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Antitubercular Agents
84 rdf:type schema:DefinedTerm
85 N23fa9c1e4db346b3a3438b4bc231c2df rdf:first sg:person.01022302627.36
86 rdf:rest N15db1d7bba644634ad65f21f94bb1046
87 N2ac9c08ac790439d9a57e151e4111754 schema:issueNumber 1
88 rdf:type schema:PublicationIssue
89 N325374bc90ab4ee592a1eb67c4837494 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Mycobacterium tuberculosis
91 rdf:type schema:DefinedTerm
92 N38399da64695487197472191745e12f1 schema:name Springer Nature - SN SciGraph project
93 rdf:type schema:Organization
94 N3fe50f14757d4a10bfc2ef9b4dca8a8f schema:name nlm_unique_id
95 schema:value 100968551
96 rdf:type schema:PropertyValue
97 N41ea1232c07d428c9260f6a7f607560b schema:volumeNumber 11
98 rdf:type schema:PublicationVolume
99 N4a756bc366ee4663a3852228d08d5588 schema:name doi
100 schema:value 10.1186/1471-2334-11-255
101 rdf:type schema:PropertyValue
102 N581f365d6bbb4bb68096de136d1f1d06 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Sequence Analysis, DNA
104 rdf:type schema:DefinedTerm
105 N7058f954f135479eb40e3160084077a2 schema:name dimensions_id
106 schema:value pub.1017063275
107 rdf:type schema:PropertyValue
108 N7a1455ef583d4ce2a09980c813ce0b61 rdf:first sg:person.0777352714.00
109 rdf:rest N23fa9c1e4db346b3a3438b4bc231c2df
110 N8a6cf9c6fba34d229b2f64fe6500ee1b rdf:first sg:person.01250162317.29
111 rdf:rest N7a1455ef583d4ce2a09980c813ce0b61
112 N8b631c9bcbbc45af909fccb7cd1aa38e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Fluoroquinolones
114 rdf:type schema:DefinedTerm
115 N9322d9b441234fdca855611220443c02 rdf:first sg:person.013107235601.50
116 rdf:rest Na774318a86624db4bc7ff351ff2e0b07
117 N974a7df2ae024e20a8dbd274ddb87ac5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Genotype
119 rdf:type schema:DefinedTerm
120 N980898bce68c4eab9661a73871bf4840 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name DNA-Directed RNA Polymerases
122 rdf:type schema:DefinedTerm
123 Na774318a86624db4bc7ff351ff2e0b07 rdf:first sg:person.01202402243.38
124 rdf:rest N1931bbdf05714b1387e9d58c58513b6b
125 Nad22f0b5c47141f986dc5408a79bd12d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Tuberculosis, Multidrug-Resistant
127 rdf:type schema:DefinedTerm
128 Nb1ec39ca839f4cedb1e114b317b58164 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Humans
130 rdf:type schema:DefinedTerm
131 Nb97710297b3945f28ee76be38a67fd1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name DNA, Bacterial
133 rdf:type schema:DefinedTerm
134 Nd54ab475ee87400bacebc21c05082079 schema:name pubmed_id
135 schema:value 21955640
136 rdf:type schema:PropertyValue
137 Ndc446d7e804a4fdb9c3c15f7d5d7160c rdf:first sg:person.0613560627.40
138 rdf:rest N8a6cf9c6fba34d229b2f64fe6500ee1b
139 Neac2d4b658d142d597d400e114b2ea9f schema:name readcube_id
140 schema:value 514be517c98129b25f572664695ed0895f97e31959c3950fc5a8b15f37492220
141 rdf:type schema:PropertyValue
142 Nf017331a79c5492380b8e34b802942bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Microbial Sensitivity Tests
144 rdf:type schema:DefinedTerm
145 Nf1b76f35f47d4e5c923ac6c75756b844 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Genes, Bacterial
147 rdf:type schema:DefinedTerm
148 Nf591db2722db41f2beb71ba0d494a891 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Phenotype
150 rdf:type schema:DefinedTerm
151 Nfa787d2376e549bbbfb2f5841ff99d26 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Cambodia
153 rdf:type schema:DefinedTerm
154 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
155 schema:name Medical and Health Sciences
156 rdf:type schema:DefinedTerm
157 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
158 schema:name Medical Microbiology
159 rdf:type schema:DefinedTerm
160 sg:grant.3776207 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2334-11-255
161 rdf:type schema:MonetaryGrant
162 sg:journal.1024946 schema:issn 1471-2334
163 schema:name BMC Infectious Diseases
164 rdf:type schema:Periodical
165 sg:person.01022302627.36 schema:affiliation https://www.grid.ac/institutes/grid.428999.7
166 schema:familyName Cadet-Daniel
167 schema:givenName Véronique
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022302627.36
169 rdf:type schema:Person
170 sg:person.01130643176.86 schema:affiliation https://www.grid.ac/institutes/grid.418537.c
171 schema:familyName Guillard
172 schema:givenName Bertrand
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130643176.86
174 rdf:type schema:Person
175 sg:person.01202402243.38 schema:affiliation https://www.grid.ac/institutes/grid.428999.7
176 schema:familyName Gicquel
177 schema:givenName Brigitte
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202402243.38
179 rdf:type schema:Person
180 sg:person.01250162317.29 schema:affiliation https://www.grid.ac/institutes/grid.418537.c
181 schema:familyName Heng
182 schema:givenName Seiha
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250162317.29
184 rdf:type schema:Person
185 sg:person.013107235601.50 schema:affiliation https://www.grid.ac/institutes/grid.428999.7
186 schema:familyName Murray
187 schema:givenName Alan
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013107235601.50
189 rdf:type schema:Person
190 sg:person.0613560627.40 schema:affiliation https://www.grid.ac/institutes/grid.418537.c
191 schema:familyName Surcouf
192 schema:givenName Corinne
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613560627.40
194 rdf:type schema:Person
195 sg:person.0743525275.80 schema:affiliation https://www.grid.ac/institutes/grid.428999.7
196 schema:familyName Namouchi
197 schema:givenName Amine
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743525275.80
199 rdf:type schema:Person
200 sg:person.0777352714.00 schema:affiliation https://www.grid.ac/institutes/grid.428999.7
201 schema:familyName Pierre-Audigier
202 schema:givenName Catherine
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777352714.00
204 rdf:type schema:Person
205 sg:pub.10.1007/s10096-003-0944-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017980690
206 https://doi.org/10.1007/s10096-003-0944-0
207 rdf:type schema:CreativeWork
208 sg:pub.10.1007/s10096-004-1268-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051992616
209 https://doi.org/10.1007/s10096-004-1268-4
210 rdf:type schema:CreativeWork
211 sg:pub.10.2165/00003495-200767140-00007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012071676
212 https://doi.org/10.2165/00003495-200767140-00007
213 rdf:type schema:CreativeWork
214 https://app.dimensions.ai/details/publication/pub.1074595044 schema:CreativeWork
215 https://app.dimensions.ai/details/publication/pub.1074836077 schema:CreativeWork
216 https://app.dimensions.ai/details/publication/pub.1077270486 schema:CreativeWork
217 https://app.dimensions.ai/details/publication/pub.1077523365 schema:CreativeWork
218 https://app.dimensions.ai/details/publication/pub.1077599504 schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1077835620 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1077878614 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1078164728 schema:CreativeWork
222 https://app.dimensions.ai/details/publication/pub.1083091586 schema:CreativeWork
223 https://doi.org/10.1016/s0140-6736(09)60333-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035006035
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/s0923-2508(02)01311-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032843541
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/s1473-3099(07)70141-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023650388
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1056/nejm199302253280802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026900723
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1056/nejm199510053331404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051126112
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1073/pnas.94.18.9869 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035792044
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1086/427292 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035959901
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1086/505877 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001797747
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1093/infdis/174.5.1127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023740802
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1093/jac/dki353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020240436
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1093/jac/dkm061 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014376523
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1111/j.1469-0691.2009.03078.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1027933747
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1128/aac.00177-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006496592
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1128/aac.40.8.1768 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082972998
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1128/aac.46.5.1417-1424.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002327339
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1128/jcm.00081-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028959980
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1128/jcm.00201-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042156203
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1128/jcm.00750-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032497579
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1177/147323000903700517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044272108
260 rdf:type schema:CreativeWork
261 https://doi.org/10.3201/eid1405.071398 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043882428
262 rdf:type schema:CreativeWork
263 https://www.grid.ac/institutes/grid.418537.c schema:alternateName Institut Pasteur du Cambodge
264 schema:name Mycobacteriology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia
265 rdf:type schema:Organization
266 https://www.grid.ac/institutes/grid.428999.7 schema:alternateName Institut Pasteur
267 schema:name Unité de Génétique Mycobactérienne, Institut Pasteur, Paris, France
268 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...