Transcript and proteomic analysis of developing white lupin (Lupinus albus L.) roots View Full Text


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Article Info

DATE

2009-01-05

AUTHORS

Li Tian, Gregory J Peel, Zhentian Lei, Naveed Aziz, Xinbin Dai, Ji He, Bonnie Watson, Patrick X Zhao, Lloyd W Sumner, Richard A Dixon

ABSTRACT

BACKGROUND: White lupin (Lupinus albus L.) roots efficiently take up and accumulate (heavy) metals, adapt to phosphate deficiency by forming cluster roots, and secrete antimicrobial prenylated isoflavones during development. Genomic and proteomic approaches were applied to identify candidate genes and proteins involved in antimicrobial defense and (heavy) metal uptake and translocation. RESULTS: A cDNA library was constructed from roots of white lupin seedlings. Eight thousand clones were randomly sequenced and assembled into 2,455 unigenes, which were annotated based on homologous matches in the NCBInr protein database. A reference map of developing white lupin root proteins was established through 2-D gel electrophoresis and peptide mass fingerprinting. High quality peptide mass spectra were obtained for 170 proteins. Microsomal membrane proteins were separated by 1-D gel electrophoresis and identified by LC-MS/MS. A total of 74 proteins were putatively identified by the peptide mass fingerprinting and the LC-MS/MS methods. Genomic and proteomic analyses identified candidate genes and proteins encoding metal binding and/or transport proteins, transcription factors, ABC transporters and phenylpropanoid biosynthetic enzymes. CONCLUSION: The combined EST and protein datasets will facilitate the understanding of white lupin's response to biotic and abiotic stresses and its utility for phytoremediation. The root ESTs provided 82 perfect simple sequence repeat (SSR) markers with potential utility in breeding white lupin for enhanced agronomic traits. More... »

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1-1

References to SciGraph publications

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  • 1981-10. Mineral-element composition ofLupinus albus andLupinus angustifolius in relation to manganese accumulation in PLANT AND SOIL
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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1471-2229-9-1

    DOI

    http://dx.doi.org/10.1186/1471-2229-9-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1043373929

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19123941


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    34 schema:description BACKGROUND: White lupin (Lupinus albus L.) roots efficiently take up and accumulate (heavy) metals, adapt to phosphate deficiency by forming cluster roots, and secrete antimicrobial prenylated isoflavones during development. Genomic and proteomic approaches were applied to identify candidate genes and proteins involved in antimicrobial defense and (heavy) metal uptake and translocation. RESULTS: A cDNA library was constructed from roots of white lupin seedlings. Eight thousand clones were randomly sequenced and assembled into 2,455 unigenes, which were annotated based on homologous matches in the NCBInr protein database. A reference map of developing white lupin root proteins was established through 2-D gel electrophoresis and peptide mass fingerprinting. High quality peptide mass spectra were obtained for 170 proteins. Microsomal membrane proteins were separated by 1-D gel electrophoresis and identified by LC-MS/MS. A total of 74 proteins were putatively identified by the peptide mass fingerprinting and the LC-MS/MS methods. Genomic and proteomic analyses identified candidate genes and proteins encoding metal binding and/or transport proteins, transcription factors, ABC transporters and phenylpropanoid biosynthetic enzymes. CONCLUSION: The combined EST and protein datasets will facilitate the understanding of white lupin's response to biotic and abiotic stresses and its utility for phytoremediation. The root ESTs provided 82 perfect simple sequence repeat (SSR) markers with potential utility in breeding white lupin for enhanced agronomic traits.
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    42 ESTs
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    80 maps
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    91 peptide mass fingerprinting
    92 peptide mass spectra
    93 perfect simple sequence repeat (SSR) markers
    94 phenylpropanoid biosynthetic enzymes
    95 phytoremediation
    96 potential utility
    97 protein
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    102 quality peptide mass spectra
    103 reference map
    104 repeat markers
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    106 root ESTs
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    111 simple sequence repeat (SSR) markers
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