A quadruple mutant of Arabidopsis reveals a β-carotene hydroxylation activity for LUT1/CYP97C1 and a regulatory role of xanthophylls on determination ... View Full Text


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Article Info

DATE

2012-04-18

AUTHORS

Alessia Fiore, Luca Dall'Osto, Stefano Cazzaniga, Gianfranco Diretto, Giovanni Giuliano, Roberto Bassi

ABSTRACT

BackgroundXanthophylls are oxygenated carotenoids playing an essential role as structural components of the photosynthetic apparatus. Xanthophylls contribute to the assembly and stability of light-harvesting complex, to light absorbance and to photoprotection. The first step in xanthophyll biosynthesis from α- and β-carotene is the hydroxylation of ε- and β-rings, performed by both non-heme iron oxygenases (CHY1, CHY2) and P450 cytochromes (LUT1/CYP97C1, LUT5/CYP97A3). The Arabidopsis triple chy1chy2lut5 mutant is almost completely depleted in β-xanthophylls.ResultsHere we report on the quadruple chy1chy2lut2lut5 mutant, additionally carrying the lut2 mutation (affecting lycopene ε-cyclase). This genotype lacks lutein and yet it shows a compensatory increase in β-xanthophylls with respect to chy1chy2lut5 mutant. Mutant plants show an even stronger photosensitivity than chy1chy2lut5, a complete lack of qE, the rapidly reversible component of non-photochemical quenching, and a peculiar organization of the pigment binding complexes into thylakoids. Biochemical analysis reveals that the chy1chy2lut2lut5 mutant is depleted in Lhcb subunits and is specifically affected in Photosystem I function, showing a deficiency in PSI-LHCI supercomplexes. Moreover, by analyzing a series of single, double, triple and quadruple Arabidopsis mutants in xanthophyll biosynthesis, we show a hitherto undescribed correlation between xanthophyll levels and the PSI-PSII ratio. The decrease in the xanthophyll/carotenoid ratio causes a proportional decrease in the LHCII and PSI core levels with respect to PSII.ConclusionsThe physiological and biochemical phenotype of the chy1chy2lut2lut5 mutant shows that (i) LUT1/CYP97C1 protein reveals a major β-carotene hydroxylase activity in vivo when depleted in its preferred substrate α-carotene; (ii) xanthophylls are needed for normal level of Photosystem I and LHCII accumulation. More... »

PAGES

50

References to SciGraph publications

  • 2006-12-27. Lutein is needed for efficient chlorophyll triplet quenching in the major LHCII antenna complex of higher plants and effective photoprotection in vivounder strong light in BMC PLANT BIOLOGY
  • 2001-05. Preparation and functional characterization of thylakoids from Arabidopsis thaliana in PHOTOSYNTHESIS RESEARCH
  • 1990-09. The use of chlorophyll fluorescence nomenclature in plant stress physiology in PHOTOSYNTHESIS RESEARCH
  • 2001-02. Photoprotection in a zeaxanthin- and lutein-deficient double mutant of Arabidopsis in PHOTOSYNTHESIS RESEARCH
  • 2010-02-03. Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX in PLANT MOLECULAR BIOLOGY
  • 2001-02. Xanthophyll cycle-dependent nonphotochemical quenching in Photosystem II: Mechanistic insights gained from Arabidopsis thaliana L. mutants that lack violaxanthin deepoxidase activity and/or lutein in PHOTOSYNTHESIS RESEARCH
  • 2001-10. The protective functions of carotenoid and flavonoid pigments against excess visible radiation at chilling temperature investigated in Arabidopsisnpq and tt mutants in PLANTA
  • 2008-08-10. Strigolactone inhibition of shoot branching in NATURE
  • 2007-04-30. The Arabidopsis Spontaneous Cell Death1 gene, encoding a ζ-carotene desaturase essential for carotenoid biosynthesis, is involved in chloroplast development, photoprotection and retrograde signalling in CELL RESEARCH
  • 2004-03. Crystal structure of spinach major light-harvesting complex at 2.72 Å resolution in NATURE
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    http://scigraph.springernature.com/pub.10.1186/1471-2229-12-50

    DOI

    http://dx.doi.org/10.1186/1471-2229-12-50

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1019188408

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22513258


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