Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin ... View Full Text


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Article Info

DATE

2011-04-11

AUTHORS

Alessandro Alboresi, Luca Dall'Osto, Alessio Aprile, Petronia Carillo, Enrica Roncaglia, Luigi Cattivelli, Roberto Bassi

ABSTRACT

BackgroundReactive oxygen species (ROS) are unavoidable by-products of oxygenic photosynthesis, causing progressive oxidative damage and ultimately cell death. Despite their destructive activity they are also signalling molecules, priming the acclimatory response to stress stimuli.ResultsTo investigate this role further, we exposed wild type Arabidopsis thaliana plants and the double mutant npq1lut2 to excess light. The mutant does not produce the xanthophylls lutein and zeaxanthin, whose key roles include ROS scavenging and prevention of ROS synthesis. Biochemical analysis revealed that singlet oxygen (1O2) accumulated to higher levels in the mutant while other ROS were unaffected, allowing to define the transcriptomic signature of the acclimatory response mediated by 1O2 which is enhanced by the lack of these xanthophylls species. The group of genes differentially regulated in npq1lut2 is enriched in sequences encoding chloroplast proteins involved in cell protection against the damaging effect of ROS. Among the early fine-tuned components, are proteins involved in tetrapyrrole biosynthesis, chlorophyll catabolism, protein import, folding and turnover, synthesis and membrane insertion of photosynthetic subunits. Up to now, the flu mutant was the only biological system adopted to define the regulation of gene expression by 1O2. In this work, we propose the use of mutants accumulating 1O2 by mechanisms different from those activated in flu to better identify ROS signalling.ConclusionsWe propose that the lack of zeaxanthin and lutein leads to 1O2 accumulation and this represents a signalling pathway in the early stages of stress acclimation, beside the response to ADP/ATP ratio and to the redox state of both plastoquinone pool. Chloroplasts respond to 1O2 accumulation by undergoing a significant change in composition and function towards a fast acclimatory response. The physiological implications of this signalling specificity are discussed. More... »

PAGES

62

References to SciGraph publications

  • 2001-02. Photoprotection in a zeaxanthin- and lutein-deficient double mutant of Arabidopsis in PHOTOSYNTHESIS RESEARCH
  • 1999-04. Enzymes of chlorophyll biosynthesis in PHOTOSYNTHESIS RESEARCH
  • 1990-09. The use of chlorophyll fluorescence nomenclature in plant stress physiology in PHOTOSYNTHESIS RESEARCH
  • 1999-02. Photosynthetic control of chloroplast gene expression in NATURE
  • 2005-11-15. Glutathione, photosynthesis and the redox regulation of stress-responsive gene expression in PHOTOSYNTHESIS RESEARCH
  • 1997-10. Tobacco mutants with a decreased number of functional nia genes compensate by modifying the diurnal regulation of transcription, post-translational modification and turnover of nitrate reductase in PLANTA
  • 2003-01. Chloroplast to nucleus communication triggered by accumulation of Mg-protoporphyrinIX in NATURE
  • 1995-11. The grand design of photosynthesis: Acclimation of the photosynthetic apparatus to environmental cues in PHOTOSYNTHESIS RESEARCH
  • 2001-10. The protective functions of carotenoid and flavonoid pigments against excess visible radiation at chilling temperature investigated in Arabidopsisnpq and tt mutants in PLANTA
  • 2006-12-27. Lutein is needed for efficient chlorophyll triplet quenching in the major LHCII antenna complex of higher plants and effective photoprotection in vivounder strong light in BMC PLANT BIOLOGY
  • 2006-08-29. Selection of differentially expressed genes in microarray data analysis in THE PHARMACOGENOMICS JOURNAL
  • 2008-05. Coordination of gene expression between organellar and nuclear genomes in NATURE REVIEWS GENETICS
  • 2009-03-13. Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets in BMC PLANT BIOLOGY
  • 2001-09. Acclimation of Arabidopsis thaliana to the light environment: the existence of separate low light and high light responses in PLANTA
  • 2009-06-26. Parallel pigment and transcriptomic analysis of four barley Albina and Xantha mutants reveals the complex network of the chloroplast-dependent metabolism in PLANT MOLECULAR BIOLOGY
  • 2000-01. A pigment-binding protein essential for regulation of photosynthetic light harvesting in NATURE
  • 2006-09-22. Plant methionine sulfoxide reductase A and B multigenic families in PHOTOSYNTHESIS RESEARCH
  • 2004-10-27. Comparative profiling of lipid-soluble antioxidants and transcripts reveals two phases of photo-oxidative stress in a xanthophyll-deficient mutant of Chlamydomonas reinhardtii in MOLECULAR GENETICS AND GENOMICS
  • 1990-05. Acclimation of barley to changes in light intensity: photosynthetic electron transport activity and components in PHOTOSYNTHESIS RESEARCH
  • 2000-05. Lhc proteins and the regulation of photosynthetic light harvesting function by xanthophylls in PHOTOSYNTHESIS RESEARCH
  • 1998-08. Nitric oxide functions as a signal in plant disease resistance in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1471-2229-11-62

    DOI

    http://dx.doi.org/10.1186/1471-2229-11-62

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21481232


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