Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L.) Millspaugh] View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-12

AUTHORS

Sutapa Dutta, Giriraj Kumawat, Bikram P Singh, Deepak K Gupta, Sangeeta Singh, Vivek Dogra, Kishor Gaikwad, Tilak R Sharma, Ranjeet S Raje, Tapas K Bandhopadhya, Subhojit Datta, Mahendra N Singh, Fakrudin Bashasab, Pawan Kulwal, KB Wanjari, Rajeev K Varshney, Douglas R Cook, Nagendra K Singh

ABSTRACT

BACKGROUND: Pigeonpea [Cajanus cajan (L.) Millspaugh], one of the most important food legumes of semi-arid tropical and subtropical regions, has limited genomic resources, particularly expressed sequence based (genic) markers. We report a comprehensive set of validated genic simple sequence repeat (SSR) markers using deep transcriptome sequencing, and its application in genetic diversity analysis and mapping. RESULTS: In this study, 43,324 transcriptome shotgun assembly unigene contigs were assembled from 1.696 million 454 GS-FLX sequence reads of separate pooled cDNA libraries prepared from leaf, root, stem and immature seed of two pigeonpea varieties, Asha and UPAS 120. A total of 3,771 genic-SSR loci, excluding homopolymeric and compound repeats, were identified; of which 2,877 PCR primer pairs were designed for marker development. Dinucleotide was the most common repeat motif with a frequency of 60.41%, followed by tri- (34.52%), hexa- (2.62%), tetra- (1.67%) and pentanucleotide (0.76%) repeat motifs. Primers were synthesized and tested for 772 of these loci with repeat lengths of ≥ 18 bp. Of these, 550 markers were validated for consistent amplification in eight diverse pigeonpea varieties; 71 were found to be polymorphic on agarose gel electrophoresis. Genetic diversity analysis was done on 22 pigeonpea varieties and eight wild species using 20 highly polymorphic genic-SSR markers. The number of alleles at these loci ranged from 4-10 and the polymorphism information content values ranged from 0.46 to 0.72. Neighbor-joining dendrogram showed distinct separation of the different groups of pigeonpea cultivars and wild species. Deep transcriptome sequencing of the two parental lines helped in silico identification of polymorphic genic-SSR loci to facilitate the rapid development of an intra-species reference genetic map, a subset of which was validated for expected allelic segregation in the reference mapping population. CONCLUSION: We developed 550 validated genic-SSR markers in pigeonpea using deep transcriptome sequencing. From these, 20 highly polymorphic markers were used to evaluate the genetic relationship among species of the genus Cajanus. A comprehensive set of genic-SSR markers was developed as an important genomic resource for diversity analysis and genetic mapping in pigeonpea. More... »

PAGES

17

References to SciGraph publications

  • 2003-12. An improved method for high-quality RNA isolation from needles of adult maritime pine trees in PLANT MOLECULAR BIOLOGY REPORTER
  • 2005-11. EST versus Genomic Derived Microsatellite Markers for Genotyping Wild and Cultivated Barley in GENETIC RESOURCES AND CROP EVOLUTION
  • 2006-07. Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome in THEORETICAL AND APPLIED GENETICS
  • 2005-12. Nonrandom distribution and frequencies of genomic and EST-derived microsatellite markers in rice, wheat, and barley in BMC GENOMICS
  • 2005-04. Characterization of EST-derived microsatellites in the wheat genome and development of eSSR markers in FUNCTIONAL & INTEGRATIVE GENOMICS
  • 2005-09. Genome sequencing in microfabricated high-density picolitre reactors in NATURE
  • 2009-06. Development and analysis of EST-SSRs for flax (Linum usitatissimum L.) in THEORETICAL AND APPLIED GENETICS
  • 2008-12. BatchPrimer3: A high throughput web application for PCR and sequencing primer design in BMC BIOINFORMATICS
  • 2006-08. Low level of genetic diversity in cultivated Pigeonpea compared to its wild relatives is revealed by diversity arrays technology in THEORETICAL AND APPLIED GENETICS
  • 2007-01. Identification, characterization and utilization of EST-derived genic microsatellite markers for genome analyses of coffee and related species in THEORETICAL AND APPLIED GENETICS
  • 2006-05. Mining and characterizing microsatellites from citrus ESTs in THEORETICAL AND APPLIED GENETICS
  • 1983-08. A new gene for male sterility in pigeonpea (Cajanus cajan (L). Millsp.) in HEREDITY
  • 2006-05. AFLP Fingerprinting in Pigeonpea (Cajanus cajan (L.) Millsp.) and its Wild Relatives in GENETIC RESOURCES AND CROP EVOLUTION
  • 2008-06. Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L. in THEORETICAL AND APPLIED GENETICS
  • 2007-01. Single-copy genes define a conserved order between rice and wheat for understanding differences caused by duplication, deletion, and transposition of genes in FUNCTIONAL & INTEGRATIVE GENOMICS
  • 2003-02. Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) in THEORETICAL AND APPLIED GENETICS
  • 2008-12. Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping in BMC GENOMICS
  • 2001-05. Assessment of genotypic variation among cultivated durum wheat based on EST-SSRS and genomic SSRS in EUPHYTICA
  • 2009-12. New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.) in BMC RESEARCH NOTES
  • 2008-12. Assessment of genetic diversity of pigeonpea cultivars using RAPD analysis in BIOLOGIA PLANTARUM
  • 2010-12. The first set of EST resource for gene discovery and marker development in pigeonpea (Cajanus cajanL.) in BMC PLANT BIOLOGY
  • 2004-10. Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton in MOLECULAR GENETICS AND GENOMICS
  • 2004-07. EST derived SSR markers for comparative mapping in wheat and rice in MOLECULAR GENETICS AND GENOMICS
  • 2000-03. Analysis of SSRs derived from grape ESTs in THEORETICAL AND APPLIED GENETICS
  • 2000-03. Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.) in THEORETICAL AND APPLIED GENETICS
  • 1998-03. Genome size variation inCajanus cajan (Fabaceae): A reconsideration in PLANT SYSTEMATICS AND EVOLUTION
  • 1995-11. Genetic fingerprinting of pigeonpea [Cajanus cajan (L.) Millsp.] and its wild relatives using RAPD markers in THEORETICAL AND APPLIED GENETICS
  • 2004-04. EST-derived microsatellites from Actinidia species and their potential for mapping in THEORETICAL AND APPLIED GENETICS
  • 2010-02. Highly variable SSR markers suitable for rice genotyping using agarose gels in MOLECULAR BREEDING
  • 2004-10. Robust simple sequence repeat markers for spruce (Picea spp.) from expressed sequence tags in THEORETICAL AND APPLIED GENETICS
  • 2010-10. Pigeonpea genomics initiative (PGI): an international effort to improve crop productivity of pigeonpea (Cajanus cajan L.) in MOLECULAR BREEDING
  • 2008-06. Genetic Improvement of Pigeon Pea — A Review in TROPICAL PLANT BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1471-2229-11-17

    DOI

    http://dx.doi.org/10.1186/1471-2229-11-17

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035899201

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21251263


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    73 schema:description BACKGROUND: Pigeonpea [Cajanus cajan (L.) Millspaugh], one of the most important food legumes of semi-arid tropical and subtropical regions, has limited genomic resources, particularly expressed sequence based (genic) markers. We report a comprehensive set of validated genic simple sequence repeat (SSR) markers using deep transcriptome sequencing, and its application in genetic diversity analysis and mapping. RESULTS: In this study, 43,324 transcriptome shotgun assembly unigene contigs were assembled from 1.696 million 454 GS-FLX sequence reads of separate pooled cDNA libraries prepared from leaf, root, stem and immature seed of two pigeonpea varieties, Asha and UPAS 120. A total of 3,771 genic-SSR loci, excluding homopolymeric and compound repeats, were identified; of which 2,877 PCR primer pairs were designed for marker development. Dinucleotide was the most common repeat motif with a frequency of 60.41%, followed by tri- (34.52%), hexa- (2.62%), tetra- (1.67%) and pentanucleotide (0.76%) repeat motifs. Primers were synthesized and tested for 772 of these loci with repeat lengths of ≥ 18 bp. Of these, 550 markers were validated for consistent amplification in eight diverse pigeonpea varieties; 71 were found to be polymorphic on agarose gel electrophoresis. Genetic diversity analysis was done on 22 pigeonpea varieties and eight wild species using 20 highly polymorphic genic-SSR markers. The number of alleles at these loci ranged from 4-10 and the polymorphism information content values ranged from 0.46 to 0.72. Neighbor-joining dendrogram showed distinct separation of the different groups of pigeonpea cultivars and wild species. Deep transcriptome sequencing of the two parental lines helped in silico identification of polymorphic genic-SSR loci to facilitate the rapid development of an intra-species reference genetic map, a subset of which was validated for expected allelic segregation in the reference mapping population. CONCLUSION: We developed 550 validated genic-SSR markers in pigeonpea using deep transcriptome sequencing. From these, 20 highly polymorphic markers were used to evaluate the genetic relationship among species of the genus Cajanus. A comprehensive set of genic-SSR markers was developed as an important genomic resource for diversity analysis and genetic mapping in pigeonpea.
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