Directed neuronal differentiation of human embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2003-12

AUTHORS

Thomas C Schulz, Gail M Palmarini, Scott A Noggle, Deborah A Weiler, Maisam M Mitalipova, Brian G Condie

ABSTRACT

BACKGROUND: We have developed a culture system for the efficient and directed differentiation of human embryonic stem cells (HESCs) to neural precursors and neurons.HESC were maintained by manual passaging and were differentiated to a morphologically distinct OCT-4+/SSEA-4- monolayer cell type prior to the derivation of embryoid bodies. Embryoid bodies were grown in suspension in serum free conditions, in the presence of 50% conditioned medium from the human hepatocarcinoma cell line HepG2 (MedII). RESULTS: A neural precursor population was observed within HESC derived serum free embryoid bodies cultured in MedII conditioned medium, around 7-10 days after derivation. The neural precursors were organized into rosettes comprised of a central cavity surrounded by ring of cells, 4 to 8 cells in width. The central cells within rosettes were proliferating, as indicated by the presence of condensed mitotic chromosomes and by phosphoHistone H3 immunostaining. When plated and maintained in adherent culture, the rosettes of neural precursors were surrounded by large interwoven networks of neurites. Immunostaining demonstrated the expression of nestin in rosettes and associated non-neuronal cell types, and a radial expression of Map-2 in rosettes. Differentiated neurons expressed the markers Map-2 and Neurofilament H, and a subpopulation of the neurons expressed tyrosine hydroxylase, a marker for dopaminergic neurons. CONCLUSION: This novel directed differentiation approach led to the efficient derivation of neuronal cultures from HESCs, including the differentiation of tyrosine hydroxylase expressing neurons. HESC were morphologically differentiated to a monolayer OCT-4+ cell type, which was used to derive embryoid bodies directly into serum free conditions. Exposure to the MedII conditioned medium enhanced the derivation of neural precursors, the first example of the effect of this conditioned medium on HESC. More... »

PAGES

27

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2202-4-27

DOI

http://dx.doi.org/10.1186/1471-2202-4-27

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024033270

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/14572319


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Division", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coculture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media, Conditioned", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media, Serum-Free", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fibroblasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neurons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Georgia", 
          "id": "https://www.grid.ac/institutes/grid.213876.9", 
          "name": [
            "Department of Animal and Dairy Science, University of Georgia, 30605, Athens, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schulz", 
        "givenName": "Thomas C", 
        "id": "sg:person.01244530113.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244530113.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Synageva BioPharma (United States)", 
          "id": "https://www.grid.ac/institutes/grid.422716.4", 
          "name": [
            "Bresagen, 111 Riverbend Rd, 30602, Athens, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Palmarini", 
        "givenName": "Gail M", 
        "id": "sg:person.0717061140.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717061140.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Augusta University", 
          "id": "https://www.grid.ac/institutes/grid.410427.4", 
          "name": [
            "Department of Genetics, University of Georgia, 30605, Athens, USA", 
            "Institute of Molecular Medicine and Genetics, Medical College of Georgia, 30912, Augusta, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Noggle", 
        "givenName": "Scott A", 
        "id": "sg:person.0640733052.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640733052.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Synageva BioPharma (United States)", 
          "id": "https://www.grid.ac/institutes/grid.422716.4", 
          "name": [
            "Bresagen, 111 Riverbend Rd, 30602, Athens, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Weiler", 
        "givenName": "Deborah A", 
        "id": "sg:person.016526433567.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016526433567.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Synageva BioPharma (United States)", 
          "id": "https://www.grid.ac/institutes/grid.422716.4", 
          "name": [
            "Bresagen, 111 Riverbend Rd, 30602, Athens, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mitalipova", 
        "givenName": "Maisam M", 
        "id": "sg:person.010047447347.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010047447347.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Georgia", 
          "id": "https://www.grid.ac/institutes/grid.213876.9", 
          "name": [
            "Bresagen, 111 Riverbend Rd, 30602, Athens, USA", 
            "Department of Genetics, University of Georgia, 30605, Athens, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Condie", 
        "givenName": "Brian G", 
        "id": "sg:person.01205036164.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205036164.36"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/347762a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001147707", 
          "https://doi.org/10.1038/347762a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/exnr.2001.7832", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001522387"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(91)90385-c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006598047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/dvdy.10108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007844949"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt780", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011057005", 
          "https://doi.org/10.1038/nbt780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt780", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011057005", 
          "https://doi.org/10.1038/nbt780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt788", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013128911", 
          "https://doi.org/10.1038/nbt788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt788", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013128911", 
          "https://doi.org/10.1038/nbt788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.80.19.6126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013545053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0165-3806(01)00325-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014459043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/292154a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017487986", 
          "https://doi.org/10.1038/292154a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/exnr.1999.7319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018311210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(03)00937-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018348912"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(03)00937-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018348912"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.78.12.7634", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020059945"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1201-1134", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020783671", 
          "https://doi.org/10.1038/nbt1201-1134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1469-7580.199.parts1-2.5.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021214598"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1201-1129", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023182407", 
          "https://doi.org/10.1038/nbt1201-1129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0166-2236(99)01428-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023403222"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(01)00263-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026007371"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(93)25052-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027408289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.282.5391.1145", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028898955"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(96)00753-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029816716"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1990.tb19199.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032103757"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/74447", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033604670", 
          "https://doi.org/10.1038/74447"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/74447", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033604670", 
          "https://doi.org/10.1038/74447"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1472-6483(12)60013-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036536359"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.neuro.25.112701.142904", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041410604"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(90)90662-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043517258"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.21-5-521", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046924758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/neu.480250705", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049552198"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/76536", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051550210", 
          "https://doi.org/10.1038/76536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/76536", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051550210", 
          "https://doi.org/10.1038/76536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074997057", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075053569", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.23-12-05178.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1075307720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080020802", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082522971", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083390272", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-12", 
    "datePublishedReg": "2003-12-01", 
    "description": "BACKGROUND: We have developed a culture system for the efficient and directed differentiation of human embryonic stem cells (HESCs) to neural precursors and neurons.HESC were maintained by manual passaging and were differentiated to a morphologically distinct OCT-4+/SSEA-4- monolayer cell type prior to the derivation of embryoid bodies. Embryoid bodies were grown in suspension in serum free conditions, in the presence of 50% conditioned medium from the human hepatocarcinoma cell line HepG2 (MedII).\nRESULTS: A neural precursor population was observed within HESC derived serum free embryoid bodies cultured in MedII conditioned medium, around 7-10 days after derivation. The neural precursors were organized into rosettes comprised of a central cavity surrounded by ring of cells, 4 to 8 cells in width. The central cells within rosettes were proliferating, as indicated by the presence of condensed mitotic chromosomes and by phosphoHistone H3 immunostaining. When plated and maintained in adherent culture, the rosettes of neural precursors were surrounded by large interwoven networks of neurites. Immunostaining demonstrated the expression of nestin in rosettes and associated non-neuronal cell types, and a radial expression of Map-2 in rosettes. Differentiated neurons expressed the markers Map-2 and Neurofilament H, and a subpopulation of the neurons expressed tyrosine hydroxylase, a marker for dopaminergic neurons.\nCONCLUSION: This novel directed differentiation approach led to the efficient derivation of neuronal cultures from HESCs, including the differentiation of tyrosine hydroxylase expressing neurons. HESC were morphologically differentiated to a monolayer OCT-4+ cell type, which was used to derive embryoid bodies directly into serum free conditions. Exposure to the MedII conditioned medium enhanced the derivation of neural precursors, the first example of the effect of this conditioned medium on HESC.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1471-2202-4-27", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1024257", 
        "issn": [
          "1471-2202"
        ], 
        "name": "BMC Neuroscience", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "4"
      }
    ], 
    "name": "Directed neuronal differentiation of human embryonic stem cells", 
    "pagination": "27", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "727f94f8978837e57123dd7ee74dc4d67e4d5a774b7af556b6510da018c9e17a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "14572319"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100966986"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2202-4-27"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1024033270"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2202-4-27", 
      "https://app.dimensions.ai/details/publication/pub.1024033270"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000505.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1471-2202-4-27"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2202-4-27'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2202-4-27'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2202-4-27'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2202-4-27'


 

This table displays all metadata directly associated to this object as RDF triples.

277 TRIPLES      21 PREDICATES      78 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2202-4-27 schema:about N15b5f5c6ae134082af123e33b4f2da37
2 N22fd5410b6094bdfa4c483cd896b36ec
3 N2848f02112b74b2db13892e39bb62175
4 N32884a5900cd4aa7b5c5fbea18535fca
5 N3738c0e2344547f7b3ae6660214da2c1
6 N42d7a7a29f474ef3ab6812868f46259f
7 N4cdf2de831ea41f3b60367a885e492ea
8 N585fd56c650e4171b9f6fdbd83d023ee
9 N66ea531ad76b4aa1bf97328975bd53ff
10 N675c1dc6adc24347b05590456ce0249e
11 N859bd3321e7b48c5acd80cce11d95927
12 N8b7da2ba8b4942e3afee148b0b7aca46
13 Nb5de1925eacd479aae5714a7d41d432f
14 Ncae18552e1c2457e89225db93904dbd1
15 Ncdcd1aac66c54272bfe629a64959ec99
16 anzsrc-for:11
17 anzsrc-for:1109
18 schema:author N38d0c8b95b7c41baaf769550911ad391
19 schema:citation sg:pub.10.1038/292154a0
20 sg:pub.10.1038/347762a0
21 sg:pub.10.1038/74447
22 sg:pub.10.1038/76536
23 sg:pub.10.1038/nbt1201-1129
24 sg:pub.10.1038/nbt1201-1134
25 sg:pub.10.1038/nbt780
26 sg:pub.10.1038/nbt788
27 https://app.dimensions.ai/details/publication/pub.1074997057
28 https://app.dimensions.ai/details/publication/pub.1075053569
29 https://app.dimensions.ai/details/publication/pub.1080020802
30 https://app.dimensions.ai/details/publication/pub.1082522971
31 https://app.dimensions.ai/details/publication/pub.1083390272
32 https://doi.org/10.1002/dvdy.10108
33 https://doi.org/10.1002/neu.480250705
34 https://doi.org/10.1006/exnr.1999.7319
35 https://doi.org/10.1006/exnr.2001.7832
36 https://doi.org/10.1016/0076-6879(93)25052-4
37 https://doi.org/10.1016/0092-8674(90)90662-x
38 https://doi.org/10.1016/0092-8674(91)90385-c
39 https://doi.org/10.1016/s0006-291x(03)00937-9
40 https://doi.org/10.1016/s0165-3806(01)00325-x
41 https://doi.org/10.1016/s0166-2236(99)01428-9
42 https://doi.org/10.1016/s0896-6273(01)00263-x
43 https://doi.org/10.1016/s0960-9822(96)00753-1
44 https://doi.org/10.1016/s1472-6483(12)60013-3
45 https://doi.org/10.1046/j.1469-7580.199.parts1-2.5.x
46 https://doi.org/10.1073/pnas.78.12.7634
47 https://doi.org/10.1073/pnas.80.19.6126
48 https://doi.org/10.1111/j.1432-1033.1990.tb19199.x
49 https://doi.org/10.1126/science.282.5391.1145
50 https://doi.org/10.1146/annurev.neuro.25.112701.142904
51 https://doi.org/10.1523/jneurosci.23-12-05178.2003
52 https://doi.org/10.1634/stemcells.21-5-521
53 schema:datePublished 2003-12
54 schema:datePublishedReg 2003-12-01
55 schema:description BACKGROUND: We have developed a culture system for the efficient and directed differentiation of human embryonic stem cells (HESCs) to neural precursors and neurons.HESC were maintained by manual passaging and were differentiated to a morphologically distinct OCT-4+/SSEA-4- monolayer cell type prior to the derivation of embryoid bodies. Embryoid bodies were grown in suspension in serum free conditions, in the presence of 50% conditioned medium from the human hepatocarcinoma cell line HepG2 (MedII). RESULTS: A neural precursor population was observed within HESC derived serum free embryoid bodies cultured in MedII conditioned medium, around 7-10 days after derivation. The neural precursors were organized into rosettes comprised of a central cavity surrounded by ring of cells, 4 to 8 cells in width. The central cells within rosettes were proliferating, as indicated by the presence of condensed mitotic chromosomes and by phosphoHistone H3 immunostaining. When plated and maintained in adherent culture, the rosettes of neural precursors were surrounded by large interwoven networks of neurites. Immunostaining demonstrated the expression of nestin in rosettes and associated non-neuronal cell types, and a radial expression of Map-2 in rosettes. Differentiated neurons expressed the markers Map-2 and Neurofilament H, and a subpopulation of the neurons expressed tyrosine hydroxylase, a marker for dopaminergic neurons. CONCLUSION: This novel directed differentiation approach led to the efficient derivation of neuronal cultures from HESCs, including the differentiation of tyrosine hydroxylase expressing neurons. HESC were morphologically differentiated to a monolayer OCT-4+ cell type, which was used to derive embryoid bodies directly into serum free conditions. Exposure to the MedII conditioned medium enhanced the derivation of neural precursors, the first example of the effect of this conditioned medium on HESC.
56 schema:genre research_article
57 schema:inLanguage en
58 schema:isAccessibleForFree true
59 schema:isPartOf Nd9f6ef13b56840dd97d8b248f6155ee6
60 Nf27cae82db8140b0b4388ed79a53ac4c
61 sg:journal.1024257
62 schema:name Directed neuronal differentiation of human embryonic stem cells
63 schema:pagination 27
64 schema:productId N582e90d3189c4d76895e816b7cabd853
65 N7e08a225368a4f18b1aeb52c4007832f
66 Nc04db13ce7d0468d8b3e20a8066c6984
67 Nd209eead80114fc797f8435dbc3f51b7
68 Ne3b76078c9b54410a7e9cdf766f0fdf8
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024033270
70 https://doi.org/10.1186/1471-2202-4-27
71 schema:sdDatePublished 2019-04-10T15:49
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher N79b272ab7202456c882dbafbd65a0138
74 schema:url http://link.springer.com/10.1186%2F1471-2202-4-27
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N15b5f5c6ae134082af123e33b4f2da37 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Cell Division
80 rdf:type schema:DefinedTerm
81 N22fd5410b6094bdfa4c483cd896b36ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Culture Media, Serum-Free
83 rdf:type schema:DefinedTerm
84 N2848f02112b74b2db13892e39bb62175 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Antigens, Differentiation
86 rdf:type schema:DefinedTerm
87 N32884a5900cd4aa7b5c5fbea18535fca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Time Factors
89 rdf:type schema:DefinedTerm
90 N3738c0e2344547f7b3ae6660214da2c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Neurons
92 rdf:type schema:DefinedTerm
93 N38d0c8b95b7c41baaf769550911ad391 rdf:first sg:person.01244530113.11
94 rdf:rest N5f354d41849e49e38ad312c6560c1f1c
95 N42d7a7a29f474ef3ab6812868f46259f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Humans
97 rdf:type schema:DefinedTerm
98 N4cdf2de831ea41f3b60367a885e492ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Animals
100 rdf:type schema:DefinedTerm
101 N582e90d3189c4d76895e816b7cabd853 schema:name pubmed_id
102 schema:value 14572319
103 rdf:type schema:PropertyValue
104 N585fd56c650e4171b9f6fdbd83d023ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Cell Culture Techniques
106 rdf:type schema:DefinedTerm
107 N5f354d41849e49e38ad312c6560c1f1c rdf:first sg:person.0717061140.40
108 rdf:rest Nec189607d9614e9abb0f228d689afff4
109 N66ea531ad76b4aa1bf97328975bd53ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Cell Differentiation
111 rdf:type schema:DefinedTerm
112 N675c1dc6adc24347b05590456ce0249e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Cells, Cultured
114 rdf:type schema:DefinedTerm
115 N79b272ab7202456c882dbafbd65a0138 schema:name Springer Nature - SN SciGraph project
116 rdf:type schema:Organization
117 N7e08a225368a4f18b1aeb52c4007832f schema:name doi
118 schema:value 10.1186/1471-2202-4-27
119 rdf:type schema:PropertyValue
120 N859bd3321e7b48c5acd80cce11d95927 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Culture Media, Conditioned
122 rdf:type schema:DefinedTerm
123 N8b7da2ba8b4942e3afee148b0b7aca46 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Fibroblasts
125 rdf:type schema:DefinedTerm
126 N93f1f1050d974803a67b0503092c27f8 rdf:first sg:person.010047447347.73
127 rdf:rest Nd7bb6f4c1e3f4013b315443a2ede7379
128 Nb5de1925eacd479aae5714a7d41d432f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Stem Cells
130 rdf:type schema:DefinedTerm
131 Nc04db13ce7d0468d8b3e20a8066c6984 schema:name dimensions_id
132 schema:value pub.1024033270
133 rdf:type schema:PropertyValue
134 Ncae18552e1c2457e89225db93904dbd1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Coculture Techniques
136 rdf:type schema:DefinedTerm
137 Ncdcd1aac66c54272bfe629a64959ec99 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Mice
139 rdf:type schema:DefinedTerm
140 Ncfb4e6b05ada48ebbab0b910e41a8a65 rdf:first sg:person.016526433567.57
141 rdf:rest N93f1f1050d974803a67b0503092c27f8
142 Nd209eead80114fc797f8435dbc3f51b7 schema:name nlm_unique_id
143 schema:value 100966986
144 rdf:type schema:PropertyValue
145 Nd7bb6f4c1e3f4013b315443a2ede7379 rdf:first sg:person.01205036164.36
146 rdf:rest rdf:nil
147 Nd9f6ef13b56840dd97d8b248f6155ee6 schema:issueNumber 1
148 rdf:type schema:PublicationIssue
149 Ne3b76078c9b54410a7e9cdf766f0fdf8 schema:name readcube_id
150 schema:value 727f94f8978837e57123dd7ee74dc4d67e4d5a774b7af556b6510da018c9e17a
151 rdf:type schema:PropertyValue
152 Nec189607d9614e9abb0f228d689afff4 rdf:first sg:person.0640733052.22
153 rdf:rest Ncfb4e6b05ada48ebbab0b910e41a8a65
154 Nf27cae82db8140b0b4388ed79a53ac4c schema:volumeNumber 4
155 rdf:type schema:PublicationVolume
156 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
157 schema:name Medical and Health Sciences
158 rdf:type schema:DefinedTerm
159 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
160 schema:name Neurosciences
161 rdf:type schema:DefinedTerm
162 sg:journal.1024257 schema:issn 1471-2202
163 schema:name BMC Neuroscience
164 rdf:type schema:Periodical
165 sg:person.010047447347.73 schema:affiliation https://www.grid.ac/institutes/grid.422716.4
166 schema:familyName Mitalipova
167 schema:givenName Maisam M
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010047447347.73
169 rdf:type schema:Person
170 sg:person.01205036164.36 schema:affiliation https://www.grid.ac/institutes/grid.213876.9
171 schema:familyName Condie
172 schema:givenName Brian G
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205036164.36
174 rdf:type schema:Person
175 sg:person.01244530113.11 schema:affiliation https://www.grid.ac/institutes/grid.213876.9
176 schema:familyName Schulz
177 schema:givenName Thomas C
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244530113.11
179 rdf:type schema:Person
180 sg:person.016526433567.57 schema:affiliation https://www.grid.ac/institutes/grid.422716.4
181 schema:familyName Weiler
182 schema:givenName Deborah A
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016526433567.57
184 rdf:type schema:Person
185 sg:person.0640733052.22 schema:affiliation https://www.grid.ac/institutes/grid.410427.4
186 schema:familyName Noggle
187 schema:givenName Scott A
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640733052.22
189 rdf:type schema:Person
190 sg:person.0717061140.40 schema:affiliation https://www.grid.ac/institutes/grid.422716.4
191 schema:familyName Palmarini
192 schema:givenName Gail M
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717061140.40
194 rdf:type schema:Person
195 sg:pub.10.1038/292154a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017487986
196 https://doi.org/10.1038/292154a0
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/347762a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001147707
199 https://doi.org/10.1038/347762a0
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/74447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033604670
202 https://doi.org/10.1038/74447
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/76536 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051550210
205 https://doi.org/10.1038/76536
206 rdf:type schema:CreativeWork
207 sg:pub.10.1038/nbt1201-1129 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023182407
208 https://doi.org/10.1038/nbt1201-1129
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/nbt1201-1134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020783671
211 https://doi.org/10.1038/nbt1201-1134
212 rdf:type schema:CreativeWork
213 sg:pub.10.1038/nbt780 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011057005
214 https://doi.org/10.1038/nbt780
215 rdf:type schema:CreativeWork
216 sg:pub.10.1038/nbt788 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013128911
217 https://doi.org/10.1038/nbt788
218 rdf:type schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1074997057 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1075053569 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1080020802 schema:CreativeWork
222 https://app.dimensions.ai/details/publication/pub.1082522971 schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1083390272 schema:CreativeWork
224 https://doi.org/10.1002/dvdy.10108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007844949
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1002/neu.480250705 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049552198
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1006/exnr.1999.7319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018311210
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1006/exnr.2001.7832 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001522387
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/0076-6879(93)25052-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027408289
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/0092-8674(90)90662-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043517258
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/0092-8674(91)90385-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1006598047
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/s0006-291x(03)00937-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018348912
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/s0165-3806(01)00325-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014459043
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/s0166-2236(99)01428-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023403222
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/s0896-6273(01)00263-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026007371
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/s0960-9822(96)00753-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029816716
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/s1472-6483(12)60013-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036536359
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1046/j.1469-7580.199.parts1-2.5.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1021214598
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1073/pnas.78.12.7634 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020059945
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1073/pnas.80.19.6126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013545053
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1111/j.1432-1033.1990.tb19199.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032103757
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1126/science.282.5391.1145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028898955
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1146/annurev.neuro.25.112701.142904 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041410604
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1523/jneurosci.23-12-05178.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075307720
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1634/stemcells.21-5-521 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046924758
265 rdf:type schema:CreativeWork
266 https://www.grid.ac/institutes/grid.213876.9 schema:alternateName University of Georgia
267 schema:name Bresagen, 111 Riverbend Rd, 30602, Athens, USA
268 Department of Animal and Dairy Science, University of Georgia, 30605, Athens, USA
269 Department of Genetics, University of Georgia, 30605, Athens, USA
270 rdf:type schema:Organization
271 https://www.grid.ac/institutes/grid.410427.4 schema:alternateName Augusta University
272 schema:name Department of Genetics, University of Georgia, 30605, Athens, USA
273 Institute of Molecular Medicine and Genetics, Medical College of Georgia, 30912, Augusta, USA
274 rdf:type schema:Organization
275 https://www.grid.ac/institutes/grid.422716.4 schema:alternateName Synageva BioPharma (United States)
276 schema:name Bresagen, 111 Riverbend Rd, 30602, Athens, USA
277 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...