Promoter analysis of the rabbit POU5F1 gene and its expression in preimplantation stage embryos View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-12

AUTHORS

Julianna Kobolak, Katalin Kiss, Zsuzsanna Polgar, Solomon Mamo, Claire Rogel-Gaillard, Zsuzsanna Tancos, Istvan Bock, Arpad G Baji, Krisztina Tar, Melinda K Pirity, Andras Dinnyes

ABSTRACT

BACKGROUND: The POU5F1 gene encodes the octamer-binding transcription factor-4 (Oct4). It is crucial in the regulation of pluripotency during embryonic development and widely used as molecular marker of embryonic stem cells (ESCs). The objective of this study was to identify and to analyse the promoter region of rabbit POU5F1 gene; furthermore to examine its expression pattern in preimplantation stage rabbit embryos. RESULTS: The upstream region of rabbit POU5F1 was subcloned sequenced and four highly conserved promoter regions (CR1-4) were identified. The highest degree of similarity on sequence level was found among the conserved domains between rabbit and human. Among the enhancers the proximal enhancer region (PE-1A) exhibited the highest degree of homology (96.4%). Furthermore, the CR4 regulator domain containing the distal enhancer (DE-2A) was responsible for stem cell-specific expression. Also, BAC library screen revealed the existence of a processed pseudogene of rabbit POU5F1. The results of quantitative real-time PCR experiments showed that POU5F1 mRNA was abundantly present in oocytes and zygotes, but it was gradually reduced until the activation of the embryonic genome, thereafter a continuous increase in POU5F1 mRNA level was observed until blastocyst stage. By using the XYClone laser system the inner cell mass (ICM) and trophoblast portions of embryos were microdissected and examined separately and POU5F1 mRNA was detected in both cell types. CONCLUSION: In this study we provide a comparative sequence analysis of the regulatory region of rabbit POU5F1 gene. Our data suggest that the POU5F1 gene is strictly regulated during early mammalian development. We proposed that the well conserved CR4 region containing the DE-2A enhancer is responsible for the highly conserved ESC specific gene expression. Notably, we are the first to report that the rabbit POU5F1 is not restricted to ICM cells only, but it is expressed in trophoblast cells as well. This information may be well applicable to investigate further the possible phylogenetic role and the regulation of POU5F1 gene. More... »

PAGES

88

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2199-10-88

DOI

http://dx.doi.org/10.1186/1471-2199-10-88

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021669333

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19732419


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blastocyst", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryonic Development", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Developmental", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Octamer Transcription Factor-3", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Nucleic Acid", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Agricultural Biotechnology Institute", 
          "id": "https://www.grid.ac/institutes/grid.417744.5", 
          "name": [
            "Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Gy\u00f6rgyi A. u. 4., H-2100, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kobolak", 
        "givenName": "Julianna", 
        "id": "sg:person.0722357265.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722357265.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Orsz\u00e1gos Gy\u00f3gyint\u00e9zeti K\u00f6zpont", 
          "id": "https://www.grid.ac/institutes/grid.436369.d", 
          "name": [
            "Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Gy\u00f6rgyi A. u. 4., H-2100, G\u00f6d\u00f6ll\u0151, Hungary", 
            "National Medical Center Cell Biology Department, Dar\u00f3ci u. 24., H-1113, Budapest, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kiss", 
        "givenName": "Katalin", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Constantine the Philosopher University in Nitra", 
          "id": "https://www.grid.ac/institutes/grid.411883.7", 
          "name": [
            "Faculty of Natural Sciences, Constantine the Philosopher University, Slovakia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Polgar", 
        "givenName": "Zsuzsanna", 
        "id": "sg:person.01231477115.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231477115.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University College Dublin", 
          "id": "https://www.grid.ac/institutes/grid.7886.1", 
          "name": [
            "Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Gy\u00f6rgyi A. u. 4., H-2100, G\u00f6d\u00f6ll\u0151, Hungary", 
            "University College Dublin, Lyons Research Farm, Newcastle Co., Dublin, Ireland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mamo", 
        "givenName": "Solomon", 
        "id": "sg:person.01034561436.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034561436.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centre de Recherches de Jouy-en-Josas", 
          "id": "https://www.grid.ac/institutes/grid.417961.c", 
          "name": [
            "INRA, UMR1313, Laboratoire de G\u00e9n\u00e9tique Animale et Biologie Int\u00e9grative, 78350, Jouy en Josas, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rogel-Gaillard", 
        "givenName": "Claire", 
        "id": "sg:person.01132646430.68", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132646430.68"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Szent Istv\u00e1n University", 
          "id": "https://www.grid.ac/institutes/grid.129553.9", 
          "name": [
            "Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Gy\u00f6rgyi A. u. 4., H-2100, G\u00f6d\u00f6ll\u0151, Hungary", 
            "Molecular Animal Biotechnology Laboratory, Szent Istvan University, Pater K. u. 1., H-2103, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tancos", 
        "givenName": "Zsuzsanna", 
        "id": "sg:person.01142417331.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142417331.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "BioTalentum (Hungary)", 
          "id": "https://www.grid.ac/institutes/grid.424211.0", 
          "name": [
            "Molecular Animal Biotechnology Laboratory, Szent Istvan University, Pater K. u. 1., H-2103, G\u00f6d\u00f6ll\u0151, Hungary", 
            "BioTalentum Ltd., Aulich Lajos u. 26., H-2100, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bock", 
        "givenName": "Istvan", 
        "id": "sg:person.011775573771.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011775573771.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Agricultural Biotechnology Institute", 
          "id": "https://www.grid.ac/institutes/grid.417744.5", 
          "name": [
            "Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Gy\u00f6rgyi A. u. 4., H-2100, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Baji", 
        "givenName": "Arpad G", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "BioTalentum (Hungary)", 
          "id": "https://www.grid.ac/institutes/grid.424211.0", 
          "name": [
            "BioTalentum Ltd., Aulich Lajos u. 26., H-2100, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tar", 
        "givenName": "Krisztina", 
        "id": "sg:person.01264117115.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264117115.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "BioTalentum (Hungary)", 
          "id": "https://www.grid.ac/institutes/grid.424211.0", 
          "name": [
            "BioTalentum Ltd., Aulich Lajos u. 26., H-2100, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pirity", 
        "givenName": "Melinda K", 
        "id": "sg:person.01235561201.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235561201.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Szent Istv\u00e1n University", 
          "id": "https://www.grid.ac/institutes/grid.129553.9", 
          "name": [
            "Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Gy\u00f6rgyi A. u. 4., H-2100, G\u00f6d\u00f6ll\u0151, Hungary", 
            "Molecular Animal Biotechnology Laboratory, Szent Istvan University, Pater K. u. 1., H-2103, G\u00f6d\u00f6ll\u0151, Hungary"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dinnyes", 
        "givenName": "Andras", 
        "id": "sg:person.01125461601.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125461601.48"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1634/stemcells.2005-0611", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000222163"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/bc.2008.098", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000674398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-4781(03)00078-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001812355"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-4781(03)00078-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001812355"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/molehr/4.11.1021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002566517"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s003350010260", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009286230", 
          "https://doi.org/10.1007/s003350010260"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.25.14.6031-6046.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010655768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/physiolgenomics.90310.2008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011436305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.yexcr.2006.08.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011835683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod63.6.1698", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013556026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod63.6.1698", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013556026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0925-4773(91)90016-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014060892"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0925-4773(91)90016-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014060892"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1744", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014201433", 
          "https://doi.org/10.1038/nrm1744"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1744", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014201433", 
          "https://doi.org/10.1038/nrm1744"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1744", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014201433", 
          "https://doi.org/10.1038/nrm1744"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000016694", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014217666"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.2006-0226", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015314902"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01535", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017211357", 
          "https://doi.org/10.1038/nature01535"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01535", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017211357", 
          "https://doi.org/10.1038/nature01535"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/344435a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018230461", 
          "https://doi.org/10.1038/344435a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0402-366", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020003015", 
          "https://doi.org/10.1038/nbt0402-366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0402-366", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020003015", 
          "https://doi.org/10.1038/nbt0402-366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-213x-7-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020314686", 
          "https://doi.org/10.1186/1471-213x-7-14"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/10425170701388834", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020320243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.103.019455", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020594026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s003350010279", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021549604", 
          "https://doi.org/10.1007/s003350010279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod60.5.1093", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021694957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod60.5.1093", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021694957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1071/rd05012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021777755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.ge.19.120185.001345", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022405493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.18.8424", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023550877"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.c500415200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023932526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.c500415200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023932526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cr.7290134", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025225165", 
          "https://doi.org/10.1038/sj.cr.7290134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cr.7290134", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025225165", 
          "https://doi.org/10.1038/sj.cr.7290134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1479-5876-4-20", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025428606", 
          "https://doi.org/10.1186/1479-5876-4-20"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/20.17.4613", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028248606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/molehr/gah155", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028855309"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod60.4.821", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030452215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1083452", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037885336"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1098-2795(200102)58:2<127::aid-mrd1>3.0.co;2-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039694329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.104.029322", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041133539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(90)90597-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043021105"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2007.09.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045793407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/molehr/gan025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047607038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.c400587200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048507394"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.08.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049638413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.08.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049638413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(00)01199-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049912713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2199-9-67", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051288059", 
          "https://doi.org/10.1186/1471-2199-9-67"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.5.11.1935", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052849551"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/clo.2008.0072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059245024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.19-18-07711.1999", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074518271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077800422", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1991.tb07850.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077836700"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082886299", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-12", 
    "datePublishedReg": "2009-12-01", 
    "description": "BACKGROUND: The POU5F1 gene encodes the octamer-binding transcription factor-4 (Oct4). It is crucial in the regulation of pluripotency during embryonic development and widely used as molecular marker of embryonic stem cells (ESCs). The objective of this study was to identify and to analyse the promoter region of rabbit POU5F1 gene; furthermore to examine its expression pattern in preimplantation stage rabbit embryos.\nRESULTS: The upstream region of rabbit POU5F1 was subcloned sequenced and four highly conserved promoter regions (CR1-4) were identified. The highest degree of similarity on sequence level was found among the conserved domains between rabbit and human. Among the enhancers the proximal enhancer region (PE-1A) exhibited the highest degree of homology (96.4%). Furthermore, the CR4 regulator domain containing the distal enhancer (DE-2A) was responsible for stem cell-specific expression. Also, BAC library screen revealed the existence of a processed pseudogene of rabbit POU5F1. The results of quantitative real-time PCR experiments showed that POU5F1 mRNA was abundantly present in oocytes and zygotes, but it was gradually reduced until the activation of the embryonic genome, thereafter a continuous increase in POU5F1 mRNA level was observed until blastocyst stage. By using the XYClone laser system the inner cell mass (ICM) and trophoblast portions of embryos were microdissected and examined separately and POU5F1 mRNA was detected in both cell types.\nCONCLUSION: In this study we provide a comparative sequence analysis of the regulatory region of rabbit POU5F1 gene. Our data suggest that the POU5F1 gene is strictly regulated during early mammalian development. We proposed that the well conserved CR4 region containing the DE-2A enhancer is responsible for the highly conserved ESC specific gene expression. Notably, we are the first to report that the rabbit POU5F1 is not restricted to ICM cells only, but it is expressed in trophoblast cells as well. This information may be well applicable to investigate further the possible phylogenetic role and the regulation of POU5F1 gene.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1471-2199-10-88", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3771704", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3632427", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1356898", 
        "issn": [
          "1471-2199"
        ], 
        "name": "BMC Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "Promoter analysis of the rabbit POU5F1 gene and its expression in preimplantation stage embryos", 
    "pagination": "88", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "673e214a2a215152cc2e8ecf41c38669a20aef3dd9e0d304c3397e0688161a84"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19732419"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100966983"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2199-10-88"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1021669333"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2199-10-88", 
      "https://app.dimensions.ai/details/publication/pub.1021669333"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T01:05", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000505.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1471-2199-10-88"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-88'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-88'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-88'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-88'


 

This table displays all metadata directly associated to this object as RDF triples.

356 TRIPLES      21 PREDICATES      87 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2199-10-88 schema:about N0cf01d3fa2ca4f5d95bd2ac15c091aec
2 N1bbccb6b18054eb293c27a8739ba594e
3 N299f64f090754cda9b7c6bdd4563ed77
4 N41617668a5184c40958ade7069014dee
5 N462134ad4af840fc8afd93bc4952c679
6 N49fbd170f9da4eafa5eb0a6766d84b8e
7 N7052fd1888124d60b2d8bdba63d9ec38
8 N90aa6a8142d84e95bd501992b7a6f6ed
9 N91c2cf0949fe47238a28fda2237b0ad5
10 Nb8e5d2d479d341cd9000625376521bd0
11 Nd786bfacfaea4ea09b5ee2c7651e4946
12 Ne886b6d0e5da4e27ad872690aa63e36d
13 anzsrc-for:06
14 anzsrc-for:0604
15 schema:author Nb26242424910491a9e4c0ba7f26d9fa5
16 schema:citation sg:pub.10.1007/s003350010260
17 sg:pub.10.1007/s003350010279
18 sg:pub.10.1038/344435a0
19 sg:pub.10.1038/nature01535
20 sg:pub.10.1038/nbt0402-366
21 sg:pub.10.1038/nrm1744
22 sg:pub.10.1038/sj.cr.7290134
23 sg:pub.10.1186/1471-213x-7-14
24 sg:pub.10.1186/1471-2199-9-67
25 sg:pub.10.1186/1479-5876-4-20
26 https://app.dimensions.ai/details/publication/pub.1077800422
27 https://app.dimensions.ai/details/publication/pub.1082886299
28 https://doi.org/10.1002/1098-2795(200102)58:2<127::aid-mrd1>3.0.co;2-a
29 https://doi.org/10.1002/j.1460-2075.1991.tb07850.x
30 https://doi.org/10.1016/0092-8674(90)90597-8
31 https://doi.org/10.1016/0925-4773(91)90016-y
32 https://doi.org/10.1016/j.cell.2005.08.020
33 https://doi.org/10.1016/j.stem.2007.09.003
34 https://doi.org/10.1016/j.yexcr.2006.08.013
35 https://doi.org/10.1016/s0014-5793(00)01199-6
36 https://doi.org/10.1016/s0167-4781(03)00078-2
37 https://doi.org/10.1071/rd05012
38 https://doi.org/10.1073/pnas.90.18.8424
39 https://doi.org/10.1074/jbc.c400587200
40 https://doi.org/10.1074/jbc.c500415200
41 https://doi.org/10.1080/10425170701388834
42 https://doi.org/10.1089/clo.2008.0072
43 https://doi.org/10.1093/molehr/4.11.1021
44 https://doi.org/10.1093/molehr/gah155
45 https://doi.org/10.1093/molehr/gan025
46 https://doi.org/10.1093/nar/20.17.4613
47 https://doi.org/10.1095/biolreprod.103.019455
48 https://doi.org/10.1095/biolreprod.104.029322
49 https://doi.org/10.1095/biolreprod60.4.821
50 https://doi.org/10.1095/biolreprod60.5.1093
51 https://doi.org/10.1095/biolreprod63.6.1698
52 https://doi.org/10.1101/gad.5.11.1935
53 https://doi.org/10.1126/science.1083452
54 https://doi.org/10.1128/mcb.25.14.6031-6046.2005
55 https://doi.org/10.1146/annurev.ge.19.120185.001345
56 https://doi.org/10.1152/physiolgenomics.90310.2008
57 https://doi.org/10.1159/000016694
58 https://doi.org/10.1515/bc.2008.098
59 https://doi.org/10.1523/jneurosci.19-18-07711.1999
60 https://doi.org/10.1634/stemcells.2005-0611
61 https://doi.org/10.1634/stemcells.2006-0226
62 schema:datePublished 2009-12
63 schema:datePublishedReg 2009-12-01
64 schema:description BACKGROUND: The POU5F1 gene encodes the octamer-binding transcription factor-4 (Oct4). It is crucial in the regulation of pluripotency during embryonic development and widely used as molecular marker of embryonic stem cells (ESCs). The objective of this study was to identify and to analyse the promoter region of rabbit POU5F1 gene; furthermore to examine its expression pattern in preimplantation stage rabbit embryos. RESULTS: The upstream region of rabbit POU5F1 was subcloned sequenced and four highly conserved promoter regions (CR1-4) were identified. The highest degree of similarity on sequence level was found among the conserved domains between rabbit and human. Among the enhancers the proximal enhancer region (PE-1A) exhibited the highest degree of homology (96.4%). Furthermore, the CR4 regulator domain containing the distal enhancer (DE-2A) was responsible for stem cell-specific expression. Also, BAC library screen revealed the existence of a processed pseudogene of rabbit POU5F1. The results of quantitative real-time PCR experiments showed that POU5F1 mRNA was abundantly present in oocytes and zygotes, but it was gradually reduced until the activation of the embryonic genome, thereafter a continuous increase in POU5F1 mRNA level was observed until blastocyst stage. By using the XYClone laser system the inner cell mass (ICM) and trophoblast portions of embryos were microdissected and examined separately and POU5F1 mRNA was detected in both cell types. CONCLUSION: In this study we provide a comparative sequence analysis of the regulatory region of rabbit POU5F1 gene. Our data suggest that the POU5F1 gene is strictly regulated during early mammalian development. We proposed that the well conserved CR4 region containing the DE-2A enhancer is responsible for the highly conserved ESC specific gene expression. Notably, we are the first to report that the rabbit POU5F1 is not restricted to ICM cells only, but it is expressed in trophoblast cells as well. This information may be well applicable to investigate further the possible phylogenetic role and the regulation of POU5F1 gene.
65 schema:genre research_article
66 schema:inLanguage en
67 schema:isAccessibleForFree true
68 schema:isPartOf N91230bc801d442b5ad1145638ce79155
69 Nf9f83818c4574fdd8cb9c23d28195a4b
70 sg:journal.1356898
71 schema:name Promoter analysis of the rabbit POU5F1 gene and its expression in preimplantation stage embryos
72 schema:pagination 88
73 schema:productId N1b28666b544e491eb6ee43f10d4eff4a
74 N47911c1d1ba64330a0b6fb7a7698307c
75 N6399ff4df47f4aa48a8dc1389afe1c65
76 Nb35c06c582364d2497eca1d3fcd878c1
77 Ne62829edc145411a9790e121c47be92c
78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021669333
79 https://doi.org/10.1186/1471-2199-10-88
80 schema:sdDatePublished 2019-04-11T01:05
81 schema:sdLicense https://scigraph.springernature.com/explorer/license/
82 schema:sdPublisher N88b56213599641579a7e65691d71f194
83 schema:url http://link.springer.com/10.1186%2F1471-2199-10-88
84 sgo:license sg:explorer/license/
85 sgo:sdDataset articles
86 rdf:type schema:ScholarlyArticle
87 N0c9b76a771424bf7af9198c656dd4706 rdf:first sg:person.01231477115.43
88 rdf:rest N54c32fc163b54a73a223f28fc03ceeaa
89 N0cf01d3fa2ca4f5d95bd2ac15c091aec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Animals
91 rdf:type schema:DefinedTerm
92 N17b52177f53e46e8acb400f397a1d2dd rdf:first sg:person.01132646430.68
93 rdf:rest N7489dc689cd7492eb6d3b9b8e251d673
94 N1b28666b544e491eb6ee43f10d4eff4a schema:name readcube_id
95 schema:value 673e214a2a215152cc2e8ecf41c38669a20aef3dd9e0d304c3397e0688161a84
96 rdf:type schema:PropertyValue
97 N1bbccb6b18054eb293c27a8739ba594e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Base Sequence
99 rdf:type schema:DefinedTerm
100 N1ff91c7eaf774eb9ac41adc3a0ccff5b rdf:first sg:person.01235561201.40
101 rdf:rest Na7ed32a861824e4daf4f1d699d8cb86e
102 N299f64f090754cda9b7c6bdd4563ed77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Female
104 rdf:type schema:DefinedTerm
105 N41617668a5184c40958ade7069014dee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Gene Expression Regulation, Developmental
107 rdf:type schema:DefinedTerm
108 N462134ad4af840fc8afd93bc4952c679 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Blastocyst
110 rdf:type schema:DefinedTerm
111 N47911c1d1ba64330a0b6fb7a7698307c schema:name dimensions_id
112 schema:value pub.1021669333
113 rdf:type schema:PropertyValue
114 N49fbd170f9da4eafa5eb0a6766d84b8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Sequence Homology, Nucleic Acid
116 rdf:type schema:DefinedTerm
117 N54c32fc163b54a73a223f28fc03ceeaa rdf:first sg:person.01034561436.30
118 rdf:rest N17b52177f53e46e8acb400f397a1d2dd
119 N6095a44e5cac4da59d1c720fad52eb5c schema:affiliation https://www.grid.ac/institutes/grid.417744.5
120 schema:familyName Baji
121 schema:givenName Arpad G
122 rdf:type schema:Person
123 N6399ff4df47f4aa48a8dc1389afe1c65 schema:name pubmed_id
124 schema:value 19732419
125 rdf:type schema:PropertyValue
126 N66a294c33afa406787b88680bbabf636 rdf:first Ncb06a1ced134406f925d9cc3712fd28d
127 rdf:rest N0c9b76a771424bf7af9198c656dd4706
128 N7052fd1888124d60b2d8bdba63d9ec38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Embryonic Development
130 rdf:type schema:DefinedTerm
131 N70ff7f5862c445169c3477e57cead425 rdf:first sg:person.01264117115.33
132 rdf:rest N1ff91c7eaf774eb9ac41adc3a0ccff5b
133 N7489dc689cd7492eb6d3b9b8e251d673 rdf:first sg:person.01142417331.58
134 rdf:rest N82688efa853f4f9bb5821b0a6b393c0c
135 N82688efa853f4f9bb5821b0a6b393c0c rdf:first sg:person.011775573771.78
136 rdf:rest Nd3421905103c418c9b98fb1e8f7704c9
137 N88b56213599641579a7e65691d71f194 schema:name Springer Nature - SN SciGraph project
138 rdf:type schema:Organization
139 N90aa6a8142d84e95bd501992b7a6f6ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Promoter Regions, Genetic
141 rdf:type schema:DefinedTerm
142 N91230bc801d442b5ad1145638ce79155 schema:issueNumber 1
143 rdf:type schema:PublicationIssue
144 N91c2cf0949fe47238a28fda2237b0ad5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Rabbits
146 rdf:type schema:DefinedTerm
147 Na7ed32a861824e4daf4f1d699d8cb86e rdf:first sg:person.01125461601.48
148 rdf:rest rdf:nil
149 Nb26242424910491a9e4c0ba7f26d9fa5 rdf:first sg:person.0722357265.06
150 rdf:rest N66a294c33afa406787b88680bbabf636
151 Nb35c06c582364d2497eca1d3fcd878c1 schema:name doi
152 schema:value 10.1186/1471-2199-10-88
153 rdf:type schema:PropertyValue
154 Nb8e5d2d479d341cd9000625376521bd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Molecular Sequence Data
156 rdf:type schema:DefinedTerm
157 Ncb06a1ced134406f925d9cc3712fd28d schema:affiliation https://www.grid.ac/institutes/grid.436369.d
158 schema:familyName Kiss
159 schema:givenName Katalin
160 rdf:type schema:Person
161 Nd3421905103c418c9b98fb1e8f7704c9 rdf:first N6095a44e5cac4da59d1c720fad52eb5c
162 rdf:rest N70ff7f5862c445169c3477e57cead425
163 Nd786bfacfaea4ea09b5ee2c7651e4946 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Octamer Transcription Factor-3
165 rdf:type schema:DefinedTerm
166 Ne62829edc145411a9790e121c47be92c schema:name nlm_unique_id
167 schema:value 100966983
168 rdf:type schema:PropertyValue
169 Ne886b6d0e5da4e27ad872690aa63e36d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Humans
171 rdf:type schema:DefinedTerm
172 Nf9f83818c4574fdd8cb9c23d28195a4b schema:volumeNumber 10
173 rdf:type schema:PublicationVolume
174 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
175 schema:name Biological Sciences
176 rdf:type schema:DefinedTerm
177 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
178 schema:name Genetics
179 rdf:type schema:DefinedTerm
180 sg:grant.3632427 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2199-10-88
181 rdf:type schema:MonetaryGrant
182 sg:grant.3771704 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2199-10-88
183 rdf:type schema:MonetaryGrant
184 sg:journal.1356898 schema:issn 1471-2199
185 schema:name BMC Molecular Biology
186 rdf:type schema:Periodical
187 sg:person.01034561436.30 schema:affiliation https://www.grid.ac/institutes/grid.7886.1
188 schema:familyName Mamo
189 schema:givenName Solomon
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034561436.30
191 rdf:type schema:Person
192 sg:person.01125461601.48 schema:affiliation https://www.grid.ac/institutes/grid.129553.9
193 schema:familyName Dinnyes
194 schema:givenName Andras
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125461601.48
196 rdf:type schema:Person
197 sg:person.01132646430.68 schema:affiliation https://www.grid.ac/institutes/grid.417961.c
198 schema:familyName Rogel-Gaillard
199 schema:givenName Claire
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132646430.68
201 rdf:type schema:Person
202 sg:person.01142417331.58 schema:affiliation https://www.grid.ac/institutes/grid.129553.9
203 schema:familyName Tancos
204 schema:givenName Zsuzsanna
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142417331.58
206 rdf:type schema:Person
207 sg:person.011775573771.78 schema:affiliation https://www.grid.ac/institutes/grid.424211.0
208 schema:familyName Bock
209 schema:givenName Istvan
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011775573771.78
211 rdf:type schema:Person
212 sg:person.01231477115.43 schema:affiliation https://www.grid.ac/institutes/grid.411883.7
213 schema:familyName Polgar
214 schema:givenName Zsuzsanna
215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231477115.43
216 rdf:type schema:Person
217 sg:person.01235561201.40 schema:affiliation https://www.grid.ac/institutes/grid.424211.0
218 schema:familyName Pirity
219 schema:givenName Melinda K
220 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235561201.40
221 rdf:type schema:Person
222 sg:person.01264117115.33 schema:affiliation https://www.grid.ac/institutes/grid.424211.0
223 schema:familyName Tar
224 schema:givenName Krisztina
225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264117115.33
226 rdf:type schema:Person
227 sg:person.0722357265.06 schema:affiliation https://www.grid.ac/institutes/grid.417744.5
228 schema:familyName Kobolak
229 schema:givenName Julianna
230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722357265.06
231 rdf:type schema:Person
232 sg:pub.10.1007/s003350010260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009286230
233 https://doi.org/10.1007/s003350010260
234 rdf:type schema:CreativeWork
235 sg:pub.10.1007/s003350010279 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021549604
236 https://doi.org/10.1007/s003350010279
237 rdf:type schema:CreativeWork
238 sg:pub.10.1038/344435a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018230461
239 https://doi.org/10.1038/344435a0
240 rdf:type schema:CreativeWork
241 sg:pub.10.1038/nature01535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017211357
242 https://doi.org/10.1038/nature01535
243 rdf:type schema:CreativeWork
244 sg:pub.10.1038/nbt0402-366 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020003015
245 https://doi.org/10.1038/nbt0402-366
246 rdf:type schema:CreativeWork
247 sg:pub.10.1038/nrm1744 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014201433
248 https://doi.org/10.1038/nrm1744
249 rdf:type schema:CreativeWork
250 sg:pub.10.1038/sj.cr.7290134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025225165
251 https://doi.org/10.1038/sj.cr.7290134
252 rdf:type schema:CreativeWork
253 sg:pub.10.1186/1471-213x-7-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020314686
254 https://doi.org/10.1186/1471-213x-7-14
255 rdf:type schema:CreativeWork
256 sg:pub.10.1186/1471-2199-9-67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051288059
257 https://doi.org/10.1186/1471-2199-9-67
258 rdf:type schema:CreativeWork
259 sg:pub.10.1186/1479-5876-4-20 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025428606
260 https://doi.org/10.1186/1479-5876-4-20
261 rdf:type schema:CreativeWork
262 https://app.dimensions.ai/details/publication/pub.1077800422 schema:CreativeWork
263 https://app.dimensions.ai/details/publication/pub.1082886299 schema:CreativeWork
264 https://doi.org/10.1002/1098-2795(200102)58:2<127::aid-mrd1>3.0.co;2-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1039694329
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1002/j.1460-2075.1991.tb07850.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077836700
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1016/0092-8674(90)90597-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043021105
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1016/0925-4773(91)90016-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1014060892
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1016/j.cell.2005.08.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049638413
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1016/j.stem.2007.09.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045793407
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1016/j.yexcr.2006.08.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011835683
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1016/s0014-5793(00)01199-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049912713
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1016/s0167-4781(03)00078-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001812355
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1071/rd05012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021777755
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1073/pnas.90.18.8424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023550877
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1074/jbc.c400587200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048507394
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1074/jbc.c500415200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023932526
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1080/10425170701388834 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020320243
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1089/clo.2008.0072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059245024
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1093/molehr/4.11.1021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002566517
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1093/molehr/gah155 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028855309
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1093/molehr/gan025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047607038
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1093/nar/20.17.4613 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028248606
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1095/biolreprod.103.019455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020594026
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1095/biolreprod.104.029322 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041133539
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1095/biolreprod60.4.821 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030452215
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1095/biolreprod60.5.1093 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021694957
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1095/biolreprod63.6.1698 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013556026
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1101/gad.5.11.1935 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052849551
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1126/science.1083452 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037885336
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1128/mcb.25.14.6031-6046.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010655768
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1146/annurev.ge.19.120185.001345 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022405493
319 rdf:type schema:CreativeWork
320 https://doi.org/10.1152/physiolgenomics.90310.2008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011436305
321 rdf:type schema:CreativeWork
322 https://doi.org/10.1159/000016694 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014217666
323 rdf:type schema:CreativeWork
324 https://doi.org/10.1515/bc.2008.098 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000674398
325 rdf:type schema:CreativeWork
326 https://doi.org/10.1523/jneurosci.19-18-07711.1999 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074518271
327 rdf:type schema:CreativeWork
328 https://doi.org/10.1634/stemcells.2005-0611 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000222163
329 rdf:type schema:CreativeWork
330 https://doi.org/10.1634/stemcells.2006-0226 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015314902
331 rdf:type schema:CreativeWork
332 https://www.grid.ac/institutes/grid.129553.9 schema:alternateName Szent István University
333 schema:name Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Györgyi A. u. 4., H-2100, Gödöllő, Hungary
334 Molecular Animal Biotechnology Laboratory, Szent Istvan University, Pater K. u. 1., H-2103, Gödöllő, Hungary
335 rdf:type schema:Organization
336 https://www.grid.ac/institutes/grid.411883.7 schema:alternateName Constantine the Philosopher University in Nitra
337 schema:name Faculty of Natural Sciences, Constantine the Philosopher University, Slovakia
338 rdf:type schema:Organization
339 https://www.grid.ac/institutes/grid.417744.5 schema:alternateName Agricultural Biotechnology Institute
340 schema:name Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Györgyi A. u. 4., H-2100, Gödöllő, Hungary
341 rdf:type schema:Organization
342 https://www.grid.ac/institutes/grid.417961.c schema:alternateName Centre de Recherches de Jouy-en-Josas
343 schema:name INRA, UMR1313, Laboratoire de Génétique Animale et Biologie Intégrative, 78350, Jouy en Josas, France
344 rdf:type schema:Organization
345 https://www.grid.ac/institutes/grid.424211.0 schema:alternateName BioTalentum (Hungary)
346 schema:name BioTalentum Ltd., Aulich Lajos u. 26., H-2100, Gödöllő, Hungary
347 Molecular Animal Biotechnology Laboratory, Szent Istvan University, Pater K. u. 1., H-2103, Gödöllő, Hungary
348 rdf:type schema:Organization
349 https://www.grid.ac/institutes/grid.436369.d schema:alternateName Országos Gyógyintézeti Központ
350 schema:name Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Györgyi A. u. 4., H-2100, Gödöllő, Hungary
351 National Medical Center Cell Biology Department, Daróci u. 24., H-1113, Budapest, Hungary
352 rdf:type schema:Organization
353 https://www.grid.ac/institutes/grid.7886.1 schema:alternateName University College Dublin
354 schema:name Micromanipulation and Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Györgyi A. u. 4., H-2100, Gödöllő, Hungary
355 University College Dublin, Lyons Research Farm, Newcastle Co., Dublin, Ireland
356 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...