The vasa regulatory region mediates germline expression and maternal transmission of proteins in the malaria mosquito Anopheles gambiae: a versatile ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-12

AUTHORS

Philippos A Papathanos, Nikolai Windbichler, Miriam Menichelli, Austin Burt, Andrea Crisanti

ABSTRACT

BACKGROUND: Germline specific promoters are an essential component of potential vector control strategies which function by genetic drive, however suitable promoters are not currently available for the main human malaria vector Anopheles gambiae. RESULTS: We have identified the Anopheles gambiae vasa-like gene and found its expression to be specifically localized to both the male and female gonads in adult mosquitoes. We have functionally characterised using transgenic reporter lines the regulatory regions required for driving transgene expression in a pattern mirroring that of the endogenous vasa locus. Two reporter constructs indicate the existence of distinct vasa regulatory elements within the 5' untranslated regions responsible not only for the spatial and temporal but also for the sex specific germline expression. vasa driven eGFP expression in the ovary of heterozygous mosquitoes resulted in the progressive accumulation of maternal protein and transcript in developing oocytes that were then detectable in all embryos and neonatal larvae. CONCLUSION: We have characterized the vasa regulatory regions that are not only suited to drive transgenes in the early germline of both sexes but could also be utilized to manipulate the zygotic genome of developing embryos via maternal deposition of active molecules. We have used computational models to show that a homing endonuclease-based gene drive system can function in the presence of maternal deposition and describe a novel non-invasive control strategy based on early vasa driven homing endonuclease expression. More... »

PAGES

65

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2199-10-65

DOI

http://dx.doi.org/10.1186/1471-2199-10-65

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038230304

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19573226


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals, Genetically Modified", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anopheles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DEAD-box RNA Helicases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ovum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Regulatory Sequences, Nucleic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spermatogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spermatozoa", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transgenes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Imperial College London", 
          "id": "https://www.grid.ac/institutes/grid.7445.2", 
          "name": [
            "Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, SW7 2AZ, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Papathanos", 
        "givenName": "Philippos A", 
        "id": "sg:person.01314155075.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314155075.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Imperial College London", 
          "id": "https://www.grid.ac/institutes/grid.7445.2", 
          "name": [
            "Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, SW7 2AZ, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Windbichler", 
        "givenName": "Nikolai", 
        "id": "sg:person.01177726475.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177726475.65"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Imperial College London", 
          "id": "https://www.grid.ac/institutes/grid.7445.2", 
          "name": [
            "Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, SW7 2AZ, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Menichelli", 
        "givenName": "Miriam", 
        "id": "sg:person.01077072012.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077072012.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Imperial College London", 
          "id": "https://www.grid.ac/institutes/grid.7445.2", 
          "name": [
            "Dept of Biology and Centre for Population Biology, Imperial College London, Silwood Park, SL5 7PY, Ascot, Berks, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Burt", 
        "givenName": "Austin", 
        "id": "sg:person.01142167260.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142167260.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Imperial College London", 
          "id": "https://www.grid.ac/institutes/grid.7445.2", 
          "name": [
            "Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, SW7 2AZ, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Crisanti", 
        "givenName": "Andrea", 
        "id": "sg:person.01034617712.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034617712.80"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/417452a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000334279", 
          "https://doi.org/10.1038/417452a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/417452a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000334279", 
          "https://doi.org/10.1038/417452a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkl645", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000602953"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pt.2004.11.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000984865"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rspb.2002.2319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001114329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/335611a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003416492", 
          "https://doi.org/10.1038/335611a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.cellbio.12.1.365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003940720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1000291", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004448292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1870", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005461495", 
          "https://doi.org/10.1038/nrg1870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1870", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005461495", 
          "https://doi.org/10.1038/nrg1870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1152", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006635141", 
          "https://doi.org/10.1038/nbt1152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1152", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006635141", 
          "https://doi.org/10.1038/nbt1152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0925-4773(01)00654-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011309379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012756616", 
          "https://doi.org/10.1038/nature04818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012756616", 
          "https://doi.org/10.1038/nature04818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012756616", 
          "https://doi.org/10.1038/nature04818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1741-7007-5-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015981328", 
          "https://doi.org/10.1186/1741-7007-5-11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.287.5462.2474", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016878686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2006.221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018858735", 
          "https://doi.org/10.1038/nprot.2006.221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2006.221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018858735", 
          "https://doi.org/10.1038/nprot.2006.221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0701515104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020055638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.01.054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024020094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2583.2002.00336.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025937319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.0962-1075.2001.00299.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028491153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0965-1748(02)00087-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037422189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2583.2004.00557.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038697758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2583.2004.00557.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038697758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkm182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039608420"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(98)70091-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040678135"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/29.18.3757", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043449639"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35016096", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044128944", 
          "https://doi.org/10.1038/35016096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35016096", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044128944", 
          "https://doi.org/10.1038/35016096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(88)90216-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044397976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1471-4922(03)00144-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044882879"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1471-4922(03)00144-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044882879"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.108.089037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046226430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.108.089037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046226430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkl720", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046669585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ydbio.2004.08.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050334201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0611511104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050367125"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m206647200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050748920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0033583505004063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054006523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science. 1138595", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062440317"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1076181", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062446937"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1078278", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062447114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1603/0022-2585-41.3.447", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068118640"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077479960", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079580222", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082615428", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-12", 
    "datePublishedReg": "2009-12-01", 
    "description": "BACKGROUND: Germline specific promoters are an essential component of potential vector control strategies which function by genetic drive, however suitable promoters are not currently available for the main human malaria vector Anopheles gambiae.\nRESULTS: We have identified the Anopheles gambiae vasa-like gene and found its expression to be specifically localized to both the male and female gonads in adult mosquitoes. We have functionally characterised using transgenic reporter lines the regulatory regions required for driving transgene expression in a pattern mirroring that of the endogenous vasa locus. Two reporter constructs indicate the existence of distinct vasa regulatory elements within the 5' untranslated regions responsible not only for the spatial and temporal but also for the sex specific germline expression. vasa driven eGFP expression in the ovary of heterozygous mosquitoes resulted in the progressive accumulation of maternal protein and transcript in developing oocytes that were then detectable in all embryos and neonatal larvae.\nCONCLUSION: We have characterized the vasa regulatory regions that are not only suited to drive transgenes in the early germline of both sexes but could also be utilized to manipulate the zygotic genome of developing embryos via maternal deposition of active molecules. We have used computational models to show that a homing endonuclease-based gene drive system can function in the presence of maternal deposition and describe a novel non-invasive control strategy based on early vasa driven homing endonuclease expression.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1471-2199-10-65", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2766231", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1356898", 
        "issn": [
          "1471-2199"
        ], 
        "name": "BMC Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "The vasa regulatory region mediates germline expression and maternal transmission of proteins in the malaria mosquito Anopheles gambiae: a versatile tool for genetic control strategies", 
    "pagination": "65", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b61de9baec7b95f5b967c8ceb850d8b1bef60b967c0a7149d518622358c770a2"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19573226"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100966983"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2199-10-65"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1038230304"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2199-10-65", 
      "https://app.dimensions.ai/details/publication/pub.1038230304"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000506.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1471-2199-10-65"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-65'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-65'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-65'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-65'


 

This table displays all metadata directly associated to this object as RDF triples.

281 TRIPLES      21 PREDICATES      83 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2199-10-65 schema:about N22e2e29a524b489f8341edb5320df310
2 N2320d296d82a4f18a6639fc4a0f6b704
3 N3685304b177d48818d8ebbb242f8ca2b
4 N36eb95a14ceb4917a34046994d3d5c62
5 N4594965b5d8541d6b46ea950de276c2b
6 N4f55b2e3e8ef42678399940fc44b731d
7 N596d8f79c0964e16b6ae7c35bbf22e8b
8 N60a4cb4b7dc1417cac41a3efdf6fe195
9 N6ece09e4cbc24d10b92460aff3b7522d
10 N7778c325c3e549e6a998ca5a7567fbcd
11 N96762200393e4f90a61350bf3ace6afc
12 Na9da5d39c25c4d77acd9ed00474164b1
13 Nbb4ed352a4c14ce9ab85a79cbbf168f7
14 Nce113addf4df4479b7e83dac9e3bf0be
15 Ndadd39386c384e64b726c86ef3a93f89
16 anzsrc-for:06
17 anzsrc-for:0604
18 schema:author N28b70b74f9e14bd0b0a108a558cc24c2
19 schema:citation sg:pub.10.1038/335611a0
20 sg:pub.10.1038/35016096
21 sg:pub.10.1038/417452a
22 sg:pub.10.1038/nature04818
23 sg:pub.10.1038/nbt1152
24 sg:pub.10.1038/nprot.2006.221
25 sg:pub.10.1038/nrg1870
26 sg:pub.10.1186/1741-7007-5-11
27 https://app.dimensions.ai/details/publication/pub.1077479960
28 https://app.dimensions.ai/details/publication/pub.1079580222
29 https://app.dimensions.ai/details/publication/pub.1082615428
30 https://doi.org/10.1016/0092-8674(88)90216-4
31 https://doi.org/10.1016/j.cell.2006.01.054
32 https://doi.org/10.1016/j.pt.2004.11.004
33 https://doi.org/10.1016/j.ydbio.2004.08.021
34 https://doi.org/10.1016/s0925-4773(01)00654-2
35 https://doi.org/10.1016/s0960-9822(98)70091-0
36 https://doi.org/10.1016/s0965-1748(02)00087-5
37 https://doi.org/10.1016/s1471-4922(03)00144-2
38 https://doi.org/10.1017/s0033583505004063
39 https://doi.org/10.1046/j.0962-1075.2001.00299.x
40 https://doi.org/10.1046/j.1365-2583.2002.00336.x
41 https://doi.org/10.1073/pnas.0611511104
42 https://doi.org/10.1073/pnas.0701515104
43 https://doi.org/10.1074/jbc.m206647200
44 https://doi.org/10.1093/nar/29.18.3757
45 https://doi.org/10.1093/nar/gkl645
46 https://doi.org/10.1093/nar/gkl720
47 https://doi.org/10.1093/nar/gkm182
48 https://doi.org/10.1098/rspb.2002.2319
49 https://doi.org/10.1111/j.1365-2583.2004.00557.x
50 https://doi.org/10.1126/science. 1138595
51 https://doi.org/10.1126/science.1076181
52 https://doi.org/10.1126/science.1078278
53 https://doi.org/10.1126/science.287.5462.2474
54 https://doi.org/10.1146/annurev.cellbio.12.1.365
55 https://doi.org/10.1371/journal.pgen.1000291
56 https://doi.org/10.1534/genetics.108.089037
57 https://doi.org/10.1603/0022-2585-41.3.447
58 schema:datePublished 2009-12
59 schema:datePublishedReg 2009-12-01
60 schema:description BACKGROUND: Germline specific promoters are an essential component of potential vector control strategies which function by genetic drive, however suitable promoters are not currently available for the main human malaria vector Anopheles gambiae. RESULTS: We have identified the Anopheles gambiae vasa-like gene and found its expression to be specifically localized to both the male and female gonads in adult mosquitoes. We have functionally characterised using transgenic reporter lines the regulatory regions required for driving transgene expression in a pattern mirroring that of the endogenous vasa locus. Two reporter constructs indicate the existence of distinct vasa regulatory elements within the 5' untranslated regions responsible not only for the spatial and temporal but also for the sex specific germline expression. vasa driven eGFP expression in the ovary of heterozygous mosquitoes resulted in the progressive accumulation of maternal protein and transcript in developing oocytes that were then detectable in all embryos and neonatal larvae. CONCLUSION: We have characterized the vasa regulatory regions that are not only suited to drive transgenes in the early germline of both sexes but could also be utilized to manipulate the zygotic genome of developing embryos via maternal deposition of active molecules. We have used computational models to show that a homing endonuclease-based gene drive system can function in the presence of maternal deposition and describe a novel non-invasive control strategy based on early vasa driven homing endonuclease expression.
61 schema:genre research_article
62 schema:inLanguage en
63 schema:isAccessibleForFree true
64 schema:isPartOf Nbd04eafcc9564ce88a6cf5df65693cae
65 Nc835463b37b446ae9df3f5d6a830ccf6
66 sg:journal.1356898
67 schema:name The vasa regulatory region mediates germline expression and maternal transmission of proteins in the malaria mosquito Anopheles gambiae: a versatile tool for genetic control strategies
68 schema:pagination 65
69 schema:productId N38e8a0bc6b2b41c38d8d51e2b2e54f53
70 N52c13357b8ce4f429365720be138c997
71 Na5e1054cded144c187e9ee62241034c4
72 Nea1e66354cc248f8b969f248b007f3ce
73 Nff45ca509f564788aa69ffe566cb54e2
74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038230304
75 https://doi.org/10.1186/1471-2199-10-65
76 schema:sdDatePublished 2019-04-10T14:59
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher N10a33e2fbe9e4a02afe743a624ae1d1b
79 schema:url http://link.springer.com/10.1186%2F1471-2199-10-65
80 sgo:license sg:explorer/license/
81 sgo:sdDataset articles
82 rdf:type schema:ScholarlyArticle
83 N10a33e2fbe9e4a02afe743a624ae1d1b schema:name Springer Nature - SN SciGraph project
84 rdf:type schema:Organization
85 N22e2e29a524b489f8341edb5320df310 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Spermatozoa
87 rdf:type schema:DefinedTerm
88 N2320d296d82a4f18a6639fc4a0f6b704 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Genes, Reporter
90 rdf:type schema:DefinedTerm
91 N28b70b74f9e14bd0b0a108a558cc24c2 rdf:first sg:person.01314155075.61
92 rdf:rest N37652988b5344c018415d03e48959a11
93 N3685304b177d48818d8ebbb242f8ca2b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Molecular Sequence Data
95 rdf:type schema:DefinedTerm
96 N36eb95a14ceb4917a34046994d3d5c62 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Ovum
98 rdf:type schema:DefinedTerm
99 N37652988b5344c018415d03e48959a11 rdf:first sg:person.01177726475.65
100 rdf:rest Nc35592560fc14253bb7ccbee9ea72bdd
101 N38e8a0bc6b2b41c38d8d51e2b2e54f53 schema:name nlm_unique_id
102 schema:value 100966983
103 rdf:type schema:PropertyValue
104 N4594965b5d8541d6b46ea950de276c2b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Regulatory Sequences, Nucleic Acid
106 rdf:type schema:DefinedTerm
107 N4f55b2e3e8ef42678399940fc44b731d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Transgenes
109 rdf:type schema:DefinedTerm
110 N52c13357b8ce4f429365720be138c997 schema:name doi
111 schema:value 10.1186/1471-2199-10-65
112 rdf:type schema:PropertyValue
113 N596d8f79c0964e16b6ae7c35bbf22e8b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Oogenesis
115 rdf:type schema:DefinedTerm
116 N60a4cb4b7dc1417cac41a3efdf6fe195 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Male
118 rdf:type schema:DefinedTerm
119 N6ece09e4cbc24d10b92460aff3b7522d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Animals
121 rdf:type schema:DefinedTerm
122 N7778c325c3e549e6a998ca5a7567fbcd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name DEAD-box RNA Helicases
124 rdf:type schema:DefinedTerm
125 N8b739773307f4bb8895ef7f78545fb43 rdf:first sg:person.01034617712.80
126 rdf:rest rdf:nil
127 N96762200393e4f90a61350bf3ace6afc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Gene Expression
129 rdf:type schema:DefinedTerm
130 Na5e1054cded144c187e9ee62241034c4 schema:name pubmed_id
131 schema:value 19573226
132 rdf:type schema:PropertyValue
133 Na9da5d39c25c4d77acd9ed00474164b1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Spermatogenesis
135 rdf:type schema:DefinedTerm
136 Nbb4ed352a4c14ce9ab85a79cbbf168f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Anopheles
138 rdf:type schema:DefinedTerm
139 Nbd04eafcc9564ce88a6cf5df65693cae schema:issueNumber 1
140 rdf:type schema:PublicationIssue
141 Nc35592560fc14253bb7ccbee9ea72bdd rdf:first sg:person.01077072012.56
142 rdf:rest Ne045840dad164cb09038993c3d25c4bb
143 Nc835463b37b446ae9df3f5d6a830ccf6 schema:volumeNumber 10
144 rdf:type schema:PublicationVolume
145 Nce113addf4df4479b7e83dac9e3bf0be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Female
147 rdf:type schema:DefinedTerm
148 Ndadd39386c384e64b726c86ef3a93f89 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Animals, Genetically Modified
150 rdf:type schema:DefinedTerm
151 Ne045840dad164cb09038993c3d25c4bb rdf:first sg:person.01142167260.64
152 rdf:rest N8b739773307f4bb8895ef7f78545fb43
153 Nea1e66354cc248f8b969f248b007f3ce schema:name dimensions_id
154 schema:value pub.1038230304
155 rdf:type schema:PropertyValue
156 Nff45ca509f564788aa69ffe566cb54e2 schema:name readcube_id
157 schema:value b61de9baec7b95f5b967c8ceb850d8b1bef60b967c0a7149d518622358c770a2
158 rdf:type schema:PropertyValue
159 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
160 schema:name Biological Sciences
161 rdf:type schema:DefinedTerm
162 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
163 schema:name Genetics
164 rdf:type schema:DefinedTerm
165 sg:grant.2766231 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2199-10-65
166 rdf:type schema:MonetaryGrant
167 sg:journal.1356898 schema:issn 1471-2199
168 schema:name BMC Molecular Biology
169 rdf:type schema:Periodical
170 sg:person.01034617712.80 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
171 schema:familyName Crisanti
172 schema:givenName Andrea
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034617712.80
174 rdf:type schema:Person
175 sg:person.01077072012.56 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
176 schema:familyName Menichelli
177 schema:givenName Miriam
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077072012.56
179 rdf:type schema:Person
180 sg:person.01142167260.64 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
181 schema:familyName Burt
182 schema:givenName Austin
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142167260.64
184 rdf:type schema:Person
185 sg:person.01177726475.65 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
186 schema:familyName Windbichler
187 schema:givenName Nikolai
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177726475.65
189 rdf:type schema:Person
190 sg:person.01314155075.61 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
191 schema:familyName Papathanos
192 schema:givenName Philippos A
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314155075.61
194 rdf:type schema:Person
195 sg:pub.10.1038/335611a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003416492
196 https://doi.org/10.1038/335611a0
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/35016096 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044128944
199 https://doi.org/10.1038/35016096
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/417452a schema:sameAs https://app.dimensions.ai/details/publication/pub.1000334279
202 https://doi.org/10.1038/417452a
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/nature04818 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012756616
205 https://doi.org/10.1038/nature04818
206 rdf:type schema:CreativeWork
207 sg:pub.10.1038/nbt1152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006635141
208 https://doi.org/10.1038/nbt1152
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/nprot.2006.221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018858735
211 https://doi.org/10.1038/nprot.2006.221
212 rdf:type schema:CreativeWork
213 sg:pub.10.1038/nrg1870 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005461495
214 https://doi.org/10.1038/nrg1870
215 rdf:type schema:CreativeWork
216 sg:pub.10.1186/1741-7007-5-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015981328
217 https://doi.org/10.1186/1741-7007-5-11
218 rdf:type schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1077479960 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1079580222 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1082615428 schema:CreativeWork
222 https://doi.org/10.1016/0092-8674(88)90216-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044397976
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/j.cell.2006.01.054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024020094
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/j.pt.2004.11.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000984865
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.ydbio.2004.08.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050334201
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/s0925-4773(01)00654-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011309379
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/s0960-9822(98)70091-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040678135
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/s0965-1748(02)00087-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037422189
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/s1471-4922(03)00144-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044882879
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1017/s0033583505004063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054006523
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1046/j.0962-1075.2001.00299.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028491153
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1046/j.1365-2583.2002.00336.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1025937319
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1073/pnas.0611511104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050367125
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1073/pnas.0701515104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020055638
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1074/jbc.m206647200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050748920
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1093/nar/29.18.3757 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043449639
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1093/nar/gkl645 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000602953
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1093/nar/gkl720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046669585
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1093/nar/gkm182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039608420
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1098/rspb.2002.2319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001114329
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1111/j.1365-2583.2004.00557.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038697758
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1126/science. 1138595 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062440317
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1126/science.1076181 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062446937
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1126/science.1078278 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062447114
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1126/science.287.5462.2474 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016878686
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1146/annurev.cellbio.12.1.365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003940720
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1371/journal.pgen.1000291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004448292
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1534/genetics.108.089037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046226430
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1603/0022-2585-41.3.447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068118640
277 rdf:type schema:CreativeWork
278 https://www.grid.ac/institutes/grid.7445.2 schema:alternateName Imperial College London
279 schema:name Dept of Biology and Centre for Population Biology, Imperial College London, Silwood Park, SL5 7PY, Ascot, Berks, UK
280 Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, SW7 2AZ, London, UK
281 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...