The vasa regulatory region mediates germline expression and maternal transmission of proteins in the malaria mosquito Anopheles gambiae: a versatile ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-12

AUTHORS

Philippos A Papathanos, Nikolai Windbichler, Miriam Menichelli, Austin Burt, Andrea Crisanti

ABSTRACT

BACKGROUND: Germline specific promoters are an essential component of potential vector control strategies which function by genetic drive, however suitable promoters are not currently available for the main human malaria vector Anopheles gambiae. RESULTS: We have identified the Anopheles gambiae vasa-like gene and found its expression to be specifically localized to both the male and female gonads in adult mosquitoes. We have functionally characterised using transgenic reporter lines the regulatory regions required for driving transgene expression in a pattern mirroring that of the endogenous vasa locus. Two reporter constructs indicate the existence of distinct vasa regulatory elements within the 5' untranslated regions responsible not only for the spatial and temporal but also for the sex specific germline expression. vasa driven eGFP expression in the ovary of heterozygous mosquitoes resulted in the progressive accumulation of maternal protein and transcript in developing oocytes that were then detectable in all embryos and neonatal larvae. CONCLUSION: We have characterized the vasa regulatory regions that are not only suited to drive transgenes in the early germline of both sexes but could also be utilized to manipulate the zygotic genome of developing embryos via maternal deposition of active molecules. We have used computational models to show that a homing endonuclease-based gene drive system can function in the presence of maternal deposition and describe a novel non-invasive control strategy based on early vasa driven homing endonuclease expression. More... »

PAGES

65

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2199-10-65

DOI

http://dx.doi.org/10.1186/1471-2199-10-65

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038230304

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19573226


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals, Genetically Modified", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anopheles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DEAD-box RNA Helicases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ovum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Regulatory Sequences, Nucleic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spermatogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spermatozoa", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transgenes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Imperial College London", 
          "id": "https://www.grid.ac/institutes/grid.7445.2", 
          "name": [
            "Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, SW7 2AZ, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Papathanos", 
        "givenName": "Philippos A", 
        "id": "sg:person.01314155075.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314155075.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Imperial College London", 
          "id": "https://www.grid.ac/institutes/grid.7445.2", 
          "name": [
            "Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, SW7 2AZ, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Windbichler", 
        "givenName": "Nikolai", 
        "id": "sg:person.01177726475.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177726475.65"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Imperial College London", 
          "id": "https://www.grid.ac/institutes/grid.7445.2", 
          "name": [
            "Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, SW7 2AZ, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Menichelli", 
        "givenName": "Miriam", 
        "id": "sg:person.01077072012.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077072012.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Imperial College London", 
          "id": "https://www.grid.ac/institutes/grid.7445.2", 
          "name": [
            "Dept of Biology and Centre for Population Biology, Imperial College London, Silwood Park, SL5 7PY, Ascot, Berks, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Burt", 
        "givenName": "Austin", 
        "id": "sg:person.01142167260.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142167260.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Imperial College London", 
          "id": "https://www.grid.ac/institutes/grid.7445.2", 
          "name": [
            "Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, SW7 2AZ, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Crisanti", 
        "givenName": "Andrea", 
        "id": "sg:person.01034617712.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034617712.80"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/417452a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000334279", 
          "https://doi.org/10.1038/417452a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/417452a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000334279", 
          "https://doi.org/10.1038/417452a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkl645", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000602953"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pt.2004.11.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000984865"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rspb.2002.2319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001114329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/335611a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003416492", 
          "https://doi.org/10.1038/335611a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.cellbio.12.1.365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003940720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1000291", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004448292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1870", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005461495", 
          "https://doi.org/10.1038/nrg1870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1870", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005461495", 
          "https://doi.org/10.1038/nrg1870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1152", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006635141", 
          "https://doi.org/10.1038/nbt1152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1152", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006635141", 
          "https://doi.org/10.1038/nbt1152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0925-4773(01)00654-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011309379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012756616", 
          "https://doi.org/10.1038/nature04818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012756616", 
          "https://doi.org/10.1038/nature04818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012756616", 
          "https://doi.org/10.1038/nature04818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1741-7007-5-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015981328", 
          "https://doi.org/10.1186/1741-7007-5-11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.287.5462.2474", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016878686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2006.221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018858735", 
          "https://doi.org/10.1038/nprot.2006.221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2006.221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018858735", 
          "https://doi.org/10.1038/nprot.2006.221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0701515104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020055638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.01.054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024020094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2583.2002.00336.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025937319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.0962-1075.2001.00299.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028491153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0965-1748(02)00087-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037422189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2583.2004.00557.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038697758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2583.2004.00557.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038697758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkm182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039608420"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(98)70091-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040678135"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/29.18.3757", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043449639"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35016096", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044128944", 
          "https://doi.org/10.1038/35016096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35016096", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044128944", 
          "https://doi.org/10.1038/35016096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(88)90216-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044397976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1471-4922(03)00144-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044882879"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1471-4922(03)00144-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044882879"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.108.089037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046226430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.108.089037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046226430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkl720", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046669585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ydbio.2004.08.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050334201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0611511104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050367125"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m206647200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050748920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0033583505004063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054006523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science. 1138595", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062440317"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1076181", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062446937"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1078278", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062447114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1603/0022-2585-41.3.447", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068118640"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077479960", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079580222", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082615428", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-12", 
    "datePublishedReg": "2009-12-01", 
    "description": "BACKGROUND: Germline specific promoters are an essential component of potential vector control strategies which function by genetic drive, however suitable promoters are not currently available for the main human malaria vector Anopheles gambiae.\nRESULTS: We have identified the Anopheles gambiae vasa-like gene and found its expression to be specifically localized to both the male and female gonads in adult mosquitoes. We have functionally characterised using transgenic reporter lines the regulatory regions required for driving transgene expression in a pattern mirroring that of the endogenous vasa locus. Two reporter constructs indicate the existence of distinct vasa regulatory elements within the 5' untranslated regions responsible not only for the spatial and temporal but also for the sex specific germline expression. vasa driven eGFP expression in the ovary of heterozygous mosquitoes resulted in the progressive accumulation of maternal protein and transcript in developing oocytes that were then detectable in all embryos and neonatal larvae.\nCONCLUSION: We have characterized the vasa regulatory regions that are not only suited to drive transgenes in the early germline of both sexes but could also be utilized to manipulate the zygotic genome of developing embryos via maternal deposition of active molecules. We have used computational models to show that a homing endonuclease-based gene drive system can function in the presence of maternal deposition and describe a novel non-invasive control strategy based on early vasa driven homing endonuclease expression.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1471-2199-10-65", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2766231", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1356898", 
        "issn": [
          "1471-2199"
        ], 
        "name": "BMC Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "The vasa regulatory region mediates germline expression and maternal transmission of proteins in the malaria mosquito Anopheles gambiae: a versatile tool for genetic control strategies", 
    "pagination": "65", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b61de9baec7b95f5b967c8ceb850d8b1bef60b967c0a7149d518622358c770a2"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19573226"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100966983"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2199-10-65"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1038230304"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2199-10-65", 
      "https://app.dimensions.ai/details/publication/pub.1038230304"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000506.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1471-2199-10-65"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-65'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-65'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-65'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-65'


 

This table displays all metadata directly associated to this object as RDF triples.

281 TRIPLES      21 PREDICATES      83 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2199-10-65 schema:about N0a6b921b913947fcab3c5912e93d876a
2 N18f2a8b78a014abea7284b5224c35ddb
3 N23ee97cfdeca44488125f94399fed8d9
4 N2bbe1c4c6f1c4c7f85c357d16e7e0574
5 N2cf00ddde8d6427faf7504270c3d39ea
6 N40dfa79736394432a70260317834179b
7 N47916cb1258d4d71a71be3ecf3682a66
8 N61eafd47d7ed4f24ba58ba88e93744df
9 N79c4d60b87e64638b9ecb3be62cbc185
10 N84a76ab6e27c41a98d4111fdfd686e2e
11 N8730b57451e840b5bf927daf2d3decce
12 N8ecc622503cc4b5aafb5cfcf8b24a1e4
13 Na866f28bebb244bd92827a510f5e41ff
14 Nedd369f56e5647f18df95eaebe7d2674
15 Nf2c5d018e7614ff696cc1023f0c95b98
16 anzsrc-for:06
17 anzsrc-for:0604
18 schema:author Ncaf092815f8246579d54055cbbb9943b
19 schema:citation sg:pub.10.1038/335611a0
20 sg:pub.10.1038/35016096
21 sg:pub.10.1038/417452a
22 sg:pub.10.1038/nature04818
23 sg:pub.10.1038/nbt1152
24 sg:pub.10.1038/nprot.2006.221
25 sg:pub.10.1038/nrg1870
26 sg:pub.10.1186/1741-7007-5-11
27 https://app.dimensions.ai/details/publication/pub.1077479960
28 https://app.dimensions.ai/details/publication/pub.1079580222
29 https://app.dimensions.ai/details/publication/pub.1082615428
30 https://doi.org/10.1016/0092-8674(88)90216-4
31 https://doi.org/10.1016/j.cell.2006.01.054
32 https://doi.org/10.1016/j.pt.2004.11.004
33 https://doi.org/10.1016/j.ydbio.2004.08.021
34 https://doi.org/10.1016/s0925-4773(01)00654-2
35 https://doi.org/10.1016/s0960-9822(98)70091-0
36 https://doi.org/10.1016/s0965-1748(02)00087-5
37 https://doi.org/10.1016/s1471-4922(03)00144-2
38 https://doi.org/10.1017/s0033583505004063
39 https://doi.org/10.1046/j.0962-1075.2001.00299.x
40 https://doi.org/10.1046/j.1365-2583.2002.00336.x
41 https://doi.org/10.1073/pnas.0611511104
42 https://doi.org/10.1073/pnas.0701515104
43 https://doi.org/10.1074/jbc.m206647200
44 https://doi.org/10.1093/nar/29.18.3757
45 https://doi.org/10.1093/nar/gkl645
46 https://doi.org/10.1093/nar/gkl720
47 https://doi.org/10.1093/nar/gkm182
48 https://doi.org/10.1098/rspb.2002.2319
49 https://doi.org/10.1111/j.1365-2583.2004.00557.x
50 https://doi.org/10.1126/science. 1138595
51 https://doi.org/10.1126/science.1076181
52 https://doi.org/10.1126/science.1078278
53 https://doi.org/10.1126/science.287.5462.2474
54 https://doi.org/10.1146/annurev.cellbio.12.1.365
55 https://doi.org/10.1371/journal.pgen.1000291
56 https://doi.org/10.1534/genetics.108.089037
57 https://doi.org/10.1603/0022-2585-41.3.447
58 schema:datePublished 2009-12
59 schema:datePublishedReg 2009-12-01
60 schema:description BACKGROUND: Germline specific promoters are an essential component of potential vector control strategies which function by genetic drive, however suitable promoters are not currently available for the main human malaria vector Anopheles gambiae. RESULTS: We have identified the Anopheles gambiae vasa-like gene and found its expression to be specifically localized to both the male and female gonads in adult mosquitoes. We have functionally characterised using transgenic reporter lines the regulatory regions required for driving transgene expression in a pattern mirroring that of the endogenous vasa locus. Two reporter constructs indicate the existence of distinct vasa regulatory elements within the 5' untranslated regions responsible not only for the spatial and temporal but also for the sex specific germline expression. vasa driven eGFP expression in the ovary of heterozygous mosquitoes resulted in the progressive accumulation of maternal protein and transcript in developing oocytes that were then detectable in all embryos and neonatal larvae. CONCLUSION: We have characterized the vasa regulatory regions that are not only suited to drive transgenes in the early germline of both sexes but could also be utilized to manipulate the zygotic genome of developing embryos via maternal deposition of active molecules. We have used computational models to show that a homing endonuclease-based gene drive system can function in the presence of maternal deposition and describe a novel non-invasive control strategy based on early vasa driven homing endonuclease expression.
61 schema:genre research_article
62 schema:inLanguage en
63 schema:isAccessibleForFree true
64 schema:isPartOf N075bf860ec2b4e5faca30891e9f73d93
65 N8b9f5ccd7305463698e112b688319338
66 sg:journal.1356898
67 schema:name The vasa regulatory region mediates germline expression and maternal transmission of proteins in the malaria mosquito Anopheles gambiae: a versatile tool for genetic control strategies
68 schema:pagination 65
69 schema:productId N410046499a7440dfb528131d29f2368e
70 Ncdd62f32e4664a918c551cd0ea9af27f
71 Nea1754fc127d4776b745540317256d65
72 Nebd07356344749c09cdaa0daca49fc86
73 Nec6bf045a3c44b8cb767bf8425ef808f
74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038230304
75 https://doi.org/10.1186/1471-2199-10-65
76 schema:sdDatePublished 2019-04-10T14:59
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher Nd2def82708704e4cbdcc8b3ce7ac3757
79 schema:url http://link.springer.com/10.1186%2F1471-2199-10-65
80 sgo:license sg:explorer/license/
81 sgo:sdDataset articles
82 rdf:type schema:ScholarlyArticle
83 N075bf860ec2b4e5faca30891e9f73d93 schema:issueNumber 1
84 rdf:type schema:PublicationIssue
85 N0a6b921b913947fcab3c5912e93d876a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Female
87 rdf:type schema:DefinedTerm
88 N154d4bff9c434930935979d93565283f rdf:first sg:person.01177726475.65
89 rdf:rest N388d89a300d2447fabd1543476425689
90 N18f2a8b78a014abea7284b5224c35ddb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Ovum
92 rdf:type schema:DefinedTerm
93 N1dce23d6eda5434c93e0d9e7ed39ef9e rdf:first sg:person.01142167260.64
94 rdf:rest N8ea7cdf6425a49d8b9965f4c39d599e1
95 N23ee97cfdeca44488125f94399fed8d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Regulatory Sequences, Nucleic Acid
97 rdf:type schema:DefinedTerm
98 N2bbe1c4c6f1c4c7f85c357d16e7e0574 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name DEAD-box RNA Helicases
100 rdf:type schema:DefinedTerm
101 N2cf00ddde8d6427faf7504270c3d39ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Molecular Sequence Data
103 rdf:type schema:DefinedTerm
104 N388d89a300d2447fabd1543476425689 rdf:first sg:person.01077072012.56
105 rdf:rest N1dce23d6eda5434c93e0d9e7ed39ef9e
106 N40dfa79736394432a70260317834179b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Transgenes
108 rdf:type schema:DefinedTerm
109 N410046499a7440dfb528131d29f2368e schema:name dimensions_id
110 schema:value pub.1038230304
111 rdf:type schema:PropertyValue
112 N47916cb1258d4d71a71be3ecf3682a66 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Oogenesis
114 rdf:type schema:DefinedTerm
115 N61eafd47d7ed4f24ba58ba88e93744df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Spermatogenesis
117 rdf:type schema:DefinedTerm
118 N79c4d60b87e64638b9ecb3be62cbc185 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Animals, Genetically Modified
120 rdf:type schema:DefinedTerm
121 N84a76ab6e27c41a98d4111fdfd686e2e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Gene Expression
123 rdf:type schema:DefinedTerm
124 N8730b57451e840b5bf927daf2d3decce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Male
126 rdf:type schema:DefinedTerm
127 N8b9f5ccd7305463698e112b688319338 schema:volumeNumber 10
128 rdf:type schema:PublicationVolume
129 N8ea7cdf6425a49d8b9965f4c39d599e1 rdf:first sg:person.01034617712.80
130 rdf:rest rdf:nil
131 N8ecc622503cc4b5aafb5cfcf8b24a1e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Spermatozoa
133 rdf:type schema:DefinedTerm
134 Na866f28bebb244bd92827a510f5e41ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Genes, Reporter
136 rdf:type schema:DefinedTerm
137 Ncaf092815f8246579d54055cbbb9943b rdf:first sg:person.01314155075.61
138 rdf:rest N154d4bff9c434930935979d93565283f
139 Ncdd62f32e4664a918c551cd0ea9af27f schema:name readcube_id
140 schema:value b61de9baec7b95f5b967c8ceb850d8b1bef60b967c0a7149d518622358c770a2
141 rdf:type schema:PropertyValue
142 Nd2def82708704e4cbdcc8b3ce7ac3757 schema:name Springer Nature - SN SciGraph project
143 rdf:type schema:Organization
144 Nea1754fc127d4776b745540317256d65 schema:name pubmed_id
145 schema:value 19573226
146 rdf:type schema:PropertyValue
147 Nebd07356344749c09cdaa0daca49fc86 schema:name nlm_unique_id
148 schema:value 100966983
149 rdf:type schema:PropertyValue
150 Nec6bf045a3c44b8cb767bf8425ef808f schema:name doi
151 schema:value 10.1186/1471-2199-10-65
152 rdf:type schema:PropertyValue
153 Nedd369f56e5647f18df95eaebe7d2674 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Animals
155 rdf:type schema:DefinedTerm
156 Nf2c5d018e7614ff696cc1023f0c95b98 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Anopheles
158 rdf:type schema:DefinedTerm
159 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
160 schema:name Biological Sciences
161 rdf:type schema:DefinedTerm
162 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
163 schema:name Genetics
164 rdf:type schema:DefinedTerm
165 sg:grant.2766231 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2199-10-65
166 rdf:type schema:MonetaryGrant
167 sg:journal.1356898 schema:issn 1471-2199
168 schema:name BMC Molecular Biology
169 rdf:type schema:Periodical
170 sg:person.01034617712.80 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
171 schema:familyName Crisanti
172 schema:givenName Andrea
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034617712.80
174 rdf:type schema:Person
175 sg:person.01077072012.56 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
176 schema:familyName Menichelli
177 schema:givenName Miriam
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077072012.56
179 rdf:type schema:Person
180 sg:person.01142167260.64 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
181 schema:familyName Burt
182 schema:givenName Austin
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142167260.64
184 rdf:type schema:Person
185 sg:person.01177726475.65 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
186 schema:familyName Windbichler
187 schema:givenName Nikolai
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177726475.65
189 rdf:type schema:Person
190 sg:person.01314155075.61 schema:affiliation https://www.grid.ac/institutes/grid.7445.2
191 schema:familyName Papathanos
192 schema:givenName Philippos A
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314155075.61
194 rdf:type schema:Person
195 sg:pub.10.1038/335611a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003416492
196 https://doi.org/10.1038/335611a0
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/35016096 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044128944
199 https://doi.org/10.1038/35016096
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/417452a schema:sameAs https://app.dimensions.ai/details/publication/pub.1000334279
202 https://doi.org/10.1038/417452a
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/nature04818 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012756616
205 https://doi.org/10.1038/nature04818
206 rdf:type schema:CreativeWork
207 sg:pub.10.1038/nbt1152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006635141
208 https://doi.org/10.1038/nbt1152
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/nprot.2006.221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018858735
211 https://doi.org/10.1038/nprot.2006.221
212 rdf:type schema:CreativeWork
213 sg:pub.10.1038/nrg1870 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005461495
214 https://doi.org/10.1038/nrg1870
215 rdf:type schema:CreativeWork
216 sg:pub.10.1186/1741-7007-5-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015981328
217 https://doi.org/10.1186/1741-7007-5-11
218 rdf:type schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1077479960 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1079580222 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1082615428 schema:CreativeWork
222 https://doi.org/10.1016/0092-8674(88)90216-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044397976
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/j.cell.2006.01.054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024020094
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/j.pt.2004.11.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000984865
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.ydbio.2004.08.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050334201
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/s0925-4773(01)00654-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011309379
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/s0960-9822(98)70091-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040678135
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/s0965-1748(02)00087-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037422189
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/s1471-4922(03)00144-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044882879
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1017/s0033583505004063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054006523
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1046/j.0962-1075.2001.00299.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028491153
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1046/j.1365-2583.2002.00336.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1025937319
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1073/pnas.0611511104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050367125
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1073/pnas.0701515104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020055638
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1074/jbc.m206647200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050748920
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1093/nar/29.18.3757 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043449639
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1093/nar/gkl645 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000602953
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1093/nar/gkl720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046669585
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1093/nar/gkm182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039608420
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1098/rspb.2002.2319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001114329
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1111/j.1365-2583.2004.00557.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038697758
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1126/science. 1138595 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062440317
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1126/science.1076181 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062446937
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1126/science.1078278 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062447114
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1126/science.287.5462.2474 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016878686
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1146/annurev.cellbio.12.1.365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003940720
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1371/journal.pgen.1000291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004448292
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1534/genetics.108.089037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046226430
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1603/0022-2585-41.3.447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068118640
277 rdf:type schema:CreativeWork
278 https://www.grid.ac/institutes/grid.7445.2 schema:alternateName Imperial College London
279 schema:name Dept of Biology and Centre for Population Biology, Imperial College London, Silwood Park, SL5 7PY, Ascot, Berks, UK
280 Division of Cell and Molecular Biology, Imperial College London, Imperial College Road, SW7 2AZ, London, UK
281 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...