Identification of two proteins that interact with the Erp virulence factor from Mycobacterium tuberculosis by using the bacterial two-hybrid system View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-12

AUTHORS

Laura I Klepp, Marcelo Soria, Federico C Blanco, María V Bianco, María P Santangelo, Angel A Cataldi, Fabiana Bigi

ABSTRACT

BACKGROUND: The exported repetitive protein (erp) gene encodes a secreted 36-kDa protein with a central domain containing several proline-glycine-leucine-threonine-serine (PGLTS) repeats. It has been demonstrated that erp is a virulence-associated factor since the disruption of this gene impairs the growth of Mycobacterium bovis and Mycobacterium tuberculosis in mice. RESULTS: In order to elucidate the function of Erp we searched for Erp-binding proteins from M. tuberculosis by using a bacterial two-hybrid system. Our results indicate that Erp interacts specifically with two putative membrane proteins, Rv1417 and Rv2617c. Further analysis revealed that the latter two interact with each other, indicating that Rv1417, Rv2617c and Erp are connected through multiple interactions. While Rv1417 is disseminated in several Actinomycetales genera, orthologues of Rv2617c are exclusively present in members of the M. tuberculosis complex (MTC). The central and amino-terminal regions of Erp were determined to be involved in the interaction with Rv1417 and Rv2627c. Erp forms from Mycobacterium smegmatis and Mycobacterium leprae were not able to interact with Rv2617c in two-hybrid assays. Immunolocalization experiments showed that Rv1417 and Rv2617c are found on the cell membrane and Erp on the bacterial cell wall. Finally, comparative genomics and expression studies revealed a possible role of Rv1417 in riboflavin metabolism. CONCLUSION: We identified interactive partners of Erp, an M. tuberculosis protein involved in virulence, which will be the focus of future investigation to decipher the function of the Erp family protein. More... »

PAGES

3

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2199-10-3

DOI

http://dx.doi.org/10.1186/1471-2199-10-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021826079

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19159459


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Profiling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mycobacterium leprae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mycobacterium smegmatis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mycobacterium tuberculosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Two-Hybrid System Techniques", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Klepp", 
        "givenName": "Laura I", 
        "id": "sg:person.01335724306.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335724306.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Buenos Aires", 
          "id": "https://www.grid.ac/institutes/grid.7345.5", 
          "name": [
            "Microbiolog\u00eda Agr\u00edcola Facultad de Agronom\u00eda, Universidad de Buenos Aires, 1417, Buenos Aires, Av. San Martin 4453, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Soria", 
        "givenName": "Marcelo", 
        "id": "sg:person.0752313305.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752313305.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Blanco", 
        "givenName": "Federico C", 
        "id": "sg:person.01162445147.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162445147.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bianco", 
        "givenName": "Mar\u00eda V", 
        "id": "sg:person.01054757062.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054757062.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Santangelo", 
        "givenName": "Mar\u00eda P", 
        "id": "sg:person.01336242565.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336242565.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cataldi", 
        "givenName": "Angel A", 
        "id": "sg:person.0661120075.68", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661120075.68"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bigi", 
        "givenName": "Fabiana", 
        "id": "sg:person.01111426665.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111426665.19"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1128/jb.185.8.2644-2652.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000105071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0923-2508(94)90041-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001286499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0923-2508(94)90041-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001286499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.183.7.2157-2164.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005497023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tube.2008.02.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011880049"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1012013361", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tube.2004.11.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013327683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(05)80360-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013618994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.72.4.2379-2382.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024184124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2180-8-38", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025651276", 
          "https://doi.org/10.1186/1471-2180-8-38"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.10.5752", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025735342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0378-1097(03)00964-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026177817"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1462-5822.2003.00237.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041479630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.02061-05", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042271375"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1462-5822.2007.00943.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051762661"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-143-11-3599", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060369293"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-147-8-2315", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060370804"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/13500872-141-9-2123", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060380943"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.282.5389.759", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062562950"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082508719", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082988613", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-12", 
    "datePublishedReg": "2009-12-01", 
    "description": "BACKGROUND: The exported repetitive protein (erp) gene encodes a secreted 36-kDa protein with a central domain containing several proline-glycine-leucine-threonine-serine (PGLTS) repeats. It has been demonstrated that erp is a virulence-associated factor since the disruption of this gene impairs the growth of Mycobacterium bovis and Mycobacterium tuberculosis in mice.\nRESULTS: In order to elucidate the function of Erp we searched for Erp-binding proteins from M. tuberculosis by using a bacterial two-hybrid system. Our results indicate that Erp interacts specifically with two putative membrane proteins, Rv1417 and Rv2617c. Further analysis revealed that the latter two interact with each other, indicating that Rv1417, Rv2617c and Erp are connected through multiple interactions. While Rv1417 is disseminated in several Actinomycetales genera, orthologues of Rv2617c are exclusively present in members of the M. tuberculosis complex (MTC). The central and amino-terminal regions of Erp were determined to be involved in the interaction with Rv1417 and Rv2627c. Erp forms from Mycobacterium smegmatis and Mycobacterium leprae were not able to interact with Rv2617c in two-hybrid assays. Immunolocalization experiments showed that Rv1417 and Rv2617c are found on the cell membrane and Erp on the bacterial cell wall. Finally, comparative genomics and expression studies revealed a possible role of Rv1417 in riboflavin metabolism.\nCONCLUSION: We identified interactive partners of Erp, an M. tuberculosis protein involved in virulence, which will be the focus of future investigation to decipher the function of the Erp family protein.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1471-2199-10-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2424520", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1356898", 
        "issn": [
          "1471-2199"
        ], 
        "name": "BMC Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "Identification of two proteins that interact with the Erp virulence factor from Mycobacterium tuberculosis by using the bacterial two-hybrid system", 
    "pagination": "3", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "67fcadc74cbe4db342428d5f09680fcdc2677cb7598e553c38b938f4d2c9e52f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19159459"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100966983"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2199-10-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1021826079"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2199-10-3", 
      "https://app.dimensions.ai/details/publication/pub.1021826079"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:19", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000505.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1471-2199-10-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2199-10-3'


 

This table displays all metadata directly associated to this object as RDF triples.

214 TRIPLES      21 PREDICATES      57 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2199-10-3 schema:about N12bcb439e393463ebc2e6af3a2918ac6
2 N45a9b58a1c324261b9e9c75f6bad9510
3 N8e8fcf1242894a10b79165e9b7620ec6
4 Nc2954a57a9af47ef89454c0be92ca21c
5 Nc6ed0edd38d3413d9704fa0ecff68c7d
6 Ne19369f1f7814f21ba32dd87ffa41325
7 Ne2069024a56f4696848c7556820d3aeb
8 Nf6e5744a7d58425894e9d97bb24dc779
9 anzsrc-for:06
10 anzsrc-for:0601
11 schema:author N7136bd4aca4b4514bb2a6be2c21352a5
12 schema:citation sg:pub.10.1186/1471-2180-8-38
13 https://app.dimensions.ai/details/publication/pub.1012013361
14 https://app.dimensions.ai/details/publication/pub.1082508719
15 https://app.dimensions.ai/details/publication/pub.1082988613
16 https://doi.org/10.1016/0923-2508(94)90041-8
17 https://doi.org/10.1016/j.tube.2004.11.002
18 https://doi.org/10.1016/j.tube.2008.02.004
19 https://doi.org/10.1016/s0022-2836(05)80360-2
20 https://doi.org/10.1016/s0378-1097(03)00964-9
21 https://doi.org/10.1046/j.1462-5822.2003.00237.x
22 https://doi.org/10.1073/pnas.95.10.5752
23 https://doi.org/10.1099/00221287-143-11-3599
24 https://doi.org/10.1099/00221287-147-8-2315
25 https://doi.org/10.1099/13500872-141-9-2123
26 https://doi.org/10.1111/j.1462-5822.2007.00943.x
27 https://doi.org/10.1126/science.282.5389.759
28 https://doi.org/10.1128/iai.02061-05
29 https://doi.org/10.1128/iai.72.4.2379-2382.2004
30 https://doi.org/10.1128/jb.183.7.2157-2164.2001
31 https://doi.org/10.1128/jb.185.8.2644-2652.2003
32 schema:datePublished 2009-12
33 schema:datePublishedReg 2009-12-01
34 schema:description BACKGROUND: The exported repetitive protein (erp) gene encodes a secreted 36-kDa protein with a central domain containing several proline-glycine-leucine-threonine-serine (PGLTS) repeats. It has been demonstrated that erp is a virulence-associated factor since the disruption of this gene impairs the growth of Mycobacterium bovis and Mycobacterium tuberculosis in mice. RESULTS: In order to elucidate the function of Erp we searched for Erp-binding proteins from M. tuberculosis by using a bacterial two-hybrid system. Our results indicate that Erp interacts specifically with two putative membrane proteins, Rv1417 and Rv2617c. Further analysis revealed that the latter two interact with each other, indicating that Rv1417, Rv2617c and Erp are connected through multiple interactions. While Rv1417 is disseminated in several Actinomycetales genera, orthologues of Rv2617c are exclusively present in members of the M. tuberculosis complex (MTC). The central and amino-terminal regions of Erp were determined to be involved in the interaction with Rv1417 and Rv2627c. Erp forms from Mycobacterium smegmatis and Mycobacterium leprae were not able to interact with Rv2617c in two-hybrid assays. Immunolocalization experiments showed that Rv1417 and Rv2617c are found on the cell membrane and Erp on the bacterial cell wall. Finally, comparative genomics and expression studies revealed a possible role of Rv1417 in riboflavin metabolism. CONCLUSION: We identified interactive partners of Erp, an M. tuberculosis protein involved in virulence, which will be the focus of future investigation to decipher the function of the Erp family protein.
35 schema:genre research_article
36 schema:inLanguage en
37 schema:isAccessibleForFree true
38 schema:isPartOf Nbd445fe487f04d919c4af3f82986c404
39 Nda5e00b12cd1480e96029cf2d829853a
40 sg:journal.1356898
41 schema:name Identification of two proteins that interact with the Erp virulence factor from Mycobacterium tuberculosis by using the bacterial two-hybrid system
42 schema:pagination 3
43 schema:productId N2d39f370447b4f4c9fe4e8723be3d6a4
44 N5384fba9e8424b99b86d4f138bca1234
45 N6456cef581c240c6a4e7835bba59b68e
46 Nb7cdca437bfe451389ff1926e24e6c2f
47 Ne1f24667ab604e2eb353ea21df6e3af9
48 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021826079
49 https://doi.org/10.1186/1471-2199-10-3
50 schema:sdDatePublished 2019-04-10T18:19
51 schema:sdLicense https://scigraph.springernature.com/explorer/license/
52 schema:sdPublisher N58b8268725584c0eb95ab42ba7251f83
53 schema:url http://link.springer.com/10.1186%2F1471-2199-10-3
54 sgo:license sg:explorer/license/
55 sgo:sdDataset articles
56 rdf:type schema:ScholarlyArticle
57 N12bcb439e393463ebc2e6af3a2918ac6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
58 schema:name Gene Expression Profiling
59 rdf:type schema:DefinedTerm
60 N2c51ec16d7dd4cc6afdeeb64c070a6e7 schema:name Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina
61 rdf:type schema:Organization
62 N2d39f370447b4f4c9fe4e8723be3d6a4 schema:name readcube_id
63 schema:value 67fcadc74cbe4db342428d5f09680fcdc2677cb7598e553c38b938f4d2c9e52f
64 rdf:type schema:PropertyValue
65 N2d6da51bb8224637b320e79885ca23b0 schema:name Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina
66 rdf:type schema:Organization
67 N45a9b58a1c324261b9e9c75f6bad9510 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Mycobacterium tuberculosis
69 rdf:type schema:DefinedTerm
70 N5384fba9e8424b99b86d4f138bca1234 schema:name nlm_unique_id
71 schema:value 100966983
72 rdf:type schema:PropertyValue
73 N558258b651414045826fe8a733c18062 rdf:first sg:person.0752313305.76
74 rdf:rest Nf38f9050706749cfa35d248e158553d7
75 N58b8268725584c0eb95ab42ba7251f83 schema:name Springer Nature - SN SciGraph project
76 rdf:type schema:Organization
77 N6456cef581c240c6a4e7835bba59b68e schema:name dimensions_id
78 schema:value pub.1021826079
79 rdf:type schema:PropertyValue
80 N7136bd4aca4b4514bb2a6be2c21352a5 rdf:first sg:person.01335724306.38
81 rdf:rest N558258b651414045826fe8a733c18062
82 N82eb15a42bc04dff8f1ec4c1e2242b02 schema:name Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina
83 rdf:type schema:Organization
84 N8e8fcf1242894a10b79165e9b7620ec6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Membrane Proteins
86 rdf:type schema:DefinedTerm
87 Naf62394aa1e14c1fa03ba848b89b7dcf schema:name Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina
88 rdf:type schema:Organization
89 Nb7cdca437bfe451389ff1926e24e6c2f schema:name pubmed_id
90 schema:value 19159459
91 rdf:type schema:PropertyValue
92 Nbb659aa89a7f4e22a7b3cba3d0ad19a0 rdf:first sg:person.0661120075.68
93 rdf:rest Nf7d99cc8982740c9a1fe09be730b7d00
94 Nbd445fe487f04d919c4af3f82986c404 schema:volumeNumber 10
95 rdf:type schema:PublicationVolume
96 Nc2954a57a9af47ef89454c0be92ca21c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Mycobacterium smegmatis
98 rdf:type schema:DefinedTerm
99 Nc6ed0edd38d3413d9704fa0ecff68c7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Bacterial Proteins
101 rdf:type schema:DefinedTerm
102 Nca9f7a614af84c5883dbfc4d919c7636 rdf:first sg:person.01336242565.18
103 rdf:rest Nbb659aa89a7f4e22a7b3cba3d0ad19a0
104 Nd16deb7e3bef473da5d1c210f9935b90 schema:name Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina
105 rdf:type schema:Organization
106 Nda5e00b12cd1480e96029cf2d829853a schema:issueNumber 1
107 rdf:type schema:PublicationIssue
108 Ndd9b29edd5254da89d876e0d3da8019f schema:name Institute of Biotechnology, CICVyA-INTA Castelar, Nicolas Repetto and Los Reseros, 1686, Hurlingham, Argentina
109 rdf:type schema:Organization
110 Ne19369f1f7814f21ba32dd87ffa41325 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Mycobacterium leprae
112 rdf:type schema:DefinedTerm
113 Ne1f24667ab604e2eb353ea21df6e3af9 schema:name doi
114 schema:value 10.1186/1471-2199-10-3
115 rdf:type schema:PropertyValue
116 Ne2069024a56f4696848c7556820d3aeb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Two-Hybrid System Techniques
118 rdf:type schema:DefinedTerm
119 Nf38f9050706749cfa35d248e158553d7 rdf:first sg:person.01162445147.42
120 rdf:rest Nfbf3f112936d4eb5b007d8b3e3a4a61c
121 Nf6e5744a7d58425894e9d97bb24dc779 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Escherichia coli
123 rdf:type schema:DefinedTerm
124 Nf7d99cc8982740c9a1fe09be730b7d00 rdf:first sg:person.01111426665.19
125 rdf:rest rdf:nil
126 Nfbf3f112936d4eb5b007d8b3e3a4a61c rdf:first sg:person.01054757062.99
127 rdf:rest Nca9f7a614af84c5883dbfc4d919c7636
128 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
129 schema:name Biological Sciences
130 rdf:type schema:DefinedTerm
131 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
132 schema:name Biochemistry and Cell Biology
133 rdf:type schema:DefinedTerm
134 sg:grant.2424520 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2199-10-3
135 rdf:type schema:MonetaryGrant
136 sg:journal.1356898 schema:issn 1471-2199
137 schema:name BMC Molecular Biology
138 rdf:type schema:Periodical
139 sg:person.01054757062.99 schema:affiliation Nd16deb7e3bef473da5d1c210f9935b90
140 schema:familyName Bianco
141 schema:givenName María V
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054757062.99
143 rdf:type schema:Person
144 sg:person.01111426665.19 schema:affiliation N2d6da51bb8224637b320e79885ca23b0
145 schema:familyName Bigi
146 schema:givenName Fabiana
147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111426665.19
148 rdf:type schema:Person
149 sg:person.01162445147.42 schema:affiliation N2c51ec16d7dd4cc6afdeeb64c070a6e7
150 schema:familyName Blanco
151 schema:givenName Federico C
152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162445147.42
153 rdf:type schema:Person
154 sg:person.01335724306.38 schema:affiliation N82eb15a42bc04dff8f1ec4c1e2242b02
155 schema:familyName Klepp
156 schema:givenName Laura I
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335724306.38
158 rdf:type schema:Person
159 sg:person.01336242565.18 schema:affiliation Naf62394aa1e14c1fa03ba848b89b7dcf
160 schema:familyName Santangelo
161 schema:givenName María P
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336242565.18
163 rdf:type schema:Person
164 sg:person.0661120075.68 schema:affiliation Ndd9b29edd5254da89d876e0d3da8019f
165 schema:familyName Cataldi
166 schema:givenName Angel A
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661120075.68
168 rdf:type schema:Person
169 sg:person.0752313305.76 schema:affiliation https://www.grid.ac/institutes/grid.7345.5
170 schema:familyName Soria
171 schema:givenName Marcelo
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752313305.76
173 rdf:type schema:Person
174 sg:pub.10.1186/1471-2180-8-38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025651276
175 https://doi.org/10.1186/1471-2180-8-38
176 rdf:type schema:CreativeWork
177 https://app.dimensions.ai/details/publication/pub.1012013361 schema:CreativeWork
178 https://app.dimensions.ai/details/publication/pub.1082508719 schema:CreativeWork
179 https://app.dimensions.ai/details/publication/pub.1082988613 schema:CreativeWork
180 https://doi.org/10.1016/0923-2508(94)90041-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001286499
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/j.tube.2004.11.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013327683
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1016/j.tube.2008.02.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011880049
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1016/s0022-2836(05)80360-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013618994
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/s0378-1097(03)00964-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026177817
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1046/j.1462-5822.2003.00237.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041479630
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1073/pnas.95.10.5752 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025735342
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1099/00221287-143-11-3599 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060369293
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1099/00221287-147-8-2315 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060370804
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1099/13500872-141-9-2123 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060380943
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1111/j.1462-5822.2007.00943.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051762661
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1126/science.282.5389.759 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062562950
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1128/iai.02061-05 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042271375
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1128/iai.72.4.2379-2382.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024184124
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1128/jb.183.7.2157-2164.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005497023
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1128/jb.185.8.2644-2652.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000105071
211 rdf:type schema:CreativeWork
212 https://www.grid.ac/institutes/grid.7345.5 schema:alternateName University of Buenos Aires
213 schema:name Microbiología Agrícola Facultad de Agronomía, Universidad de Buenos Aires, 1417, Buenos Aires, Av. San Martin 4453, Argentina
214 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...