Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolates View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-10-08

AUTHORS

Luanne Hall-Stoodley, Laura Nistico, Karthik Sambanthamoorthy, Bethany Dice, Duc Nguyen, William J Mershon, Candice Johnson, Fen Ze Hu, Paul Stoodley, Garth D Ehrlich, J Christopher Post

ABSTRACT

BACKGROUND: Streptococcus pneumoniae is a common respiratory pathogen and a major causative agent of respiratory infections, including otitis media (OM). Pneumococcal biofilms have been demonstrated on biopsies of the middle ear mucosa in children receiving tympanostomy tubes, supporting the hypothesis that chronic OM may involve biofilm development by pathogenic bacteria as part of the infectious process. To better understand pneumococcal biofilm formation six low-passage encapsulated nasopharyngeal isolates of S. pneumoniae were assessed over a six-eight day period in vitro. RESULTS: Multiparametric analysis divided the strains into two groups. Those with a high biofilm forming index (BFI) were structurally complex, exhibited greater lectin colocalization and were more resistant to azithromycin. Those with a low BFI developed less extensive biofilms and were more susceptible to azithromycin. dsDNA was present in the S. pneumoniae biofilm matrix in all strains and treatment with DNase I significantly reduced biofilm biomass. Since capsule expression has been hypothesized to be associated with decreased biofilm development, we also examined expression of cpsA, the first gene in the pneumococcal capsule operon. Interestingly, cpsA was downregulated in biofilms in both high and low BFI strains. CONCLUSION: All pneumococcal strains developed biofilms that exhibited extracellular dsDNA in the biofilm matrix, however strains with a high BFI correlated with greater carbohydrate-associated structural complexity and antibiotic resistance. Furthermore, all strains of S. pneumoniae showed downregulation of the cpsA gene during biofilm growth compared to planktonic culture, regardless of BFI ranking, suggesting downregulation of capsule expression occurs generally during adherent growth. More... »

PAGES

173-173

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2180-8-173

DOI

http://dx.doi.org/10.1186/1471-2180-8-173

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030255514

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18842140


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anti-Bacterial Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Azithromycin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biofilms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Child", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colony Count, Microbial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Deoxyribonuclease I", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Profiling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nasopharynx", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Streptococcus pneumoniae", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA", 
          "id": "http://www.grid.ac/institutes/grid.166341.7", 
          "name": [
            "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA", 
            "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hall-Stoodley", 
        "givenName": "Luanne", 
        "id": "sg:person.0607041661.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607041661.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA", 
          "id": "http://www.grid.ac/institutes/grid.280673.8", 
          "name": [
            "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nistico", 
        "givenName": "Laura", 
        "id": "sg:person.01263223327.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263223327.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA", 
          "id": "http://www.grid.ac/institutes/grid.280673.8", 
          "name": [
            "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sambanthamoorthy", 
        "givenName": "Karthik", 
        "id": "sg:person.01367600263.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367600263.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA", 
          "id": "http://www.grid.ac/institutes/grid.280673.8", 
          "name": [
            "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dice", 
        "givenName": "Bethany", 
        "id": "sg:person.01274441357.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274441357.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA", 
          "id": "http://www.grid.ac/institutes/grid.280673.8", 
          "name": [
            "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nguyen", 
        "givenName": "Duc", 
        "id": "sg:person.0644477423.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0644477423.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tescan USA Inc, 508 Thomson Park Drive, Cranberry Township, PA 16066, USA", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Tescan USA Inc, 508 Thomson Park Drive, Cranberry Township, PA 16066, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mershon", 
        "givenName": "William J", 
        "id": "sg:person.0733063361.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733063361.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA", 
          "id": "http://www.grid.ac/institutes/grid.280673.8", 
          "name": [
            "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Johnson", 
        "givenName": "Candice", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA", 
          "id": "http://www.grid.ac/institutes/grid.166341.7", 
          "name": [
            "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA", 
            "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hu", 
        "givenName": "Fen Ze", 
        "id": "sg:person.010625263442.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010625263442.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA", 
          "id": "http://www.grid.ac/institutes/grid.166341.7", 
          "name": [
            "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA", 
            "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Stoodley", 
        "givenName": "Paul", 
        "id": "sg:person.01316722264.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316722264.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA", 
          "id": "http://www.grid.ac/institutes/grid.166341.7", 
          "name": [
            "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA", 
            "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ehrlich", 
        "givenName": "Garth D", 
        "id": "sg:person.01324252605.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324252605.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA", 
          "id": "http://www.grid.ac/institutes/grid.166341.7", 
          "name": [
            "Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA", 
            "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Post", 
        "givenName": "J Christopher", 
        "id": "sg:person.01055120221.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055120221.02"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nrmicro821", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039651347", 
          "https://doi.org/10.1038/nrmicro821"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2008-10-08", 
    "datePublishedReg": "2008-10-08", 
    "description": "BACKGROUND: Streptococcus pneumoniae is a common respiratory pathogen and a major causative agent of respiratory infections, including otitis media (OM). Pneumococcal biofilms have been demonstrated on biopsies of the middle ear mucosa in children receiving tympanostomy tubes, supporting the hypothesis that chronic OM may involve biofilm development by pathogenic bacteria as part of the infectious process. To better understand pneumococcal biofilm formation six low-passage encapsulated nasopharyngeal isolates of S. pneumoniae were assessed over a six-eight day period in vitro.\nRESULTS: Multiparametric analysis divided the strains into two groups. Those with a high biofilm forming index (BFI) were structurally complex, exhibited greater lectin colocalization and were more resistant to azithromycin. Those with a low BFI developed less extensive biofilms and were more susceptible to azithromycin. dsDNA was present in the S. pneumoniae biofilm matrix in all strains and treatment with DNase I significantly reduced biofilm biomass. Since capsule expression has been hypothesized to be associated with decreased biofilm development, we also examined expression of cpsA, the first gene in the pneumococcal capsule operon. Interestingly, cpsA was downregulated in biofilms in both high and low BFI strains.\nCONCLUSION: All pneumococcal strains developed biofilms that exhibited extracellular dsDNA in the biofilm matrix, however strains with a high BFI correlated with greater carbohydrate-associated structural complexity and antibiotic resistance. Furthermore, all strains of S. pneumoniae showed downregulation of the cpsA gene during biofilm growth compared to planktonic culture, regardless of BFI ranking, suggesting downregulation of capsule expression occurs generally during adherent growth.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/1471-2180-8-173", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2488646", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2488387", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2488072", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1024253", 
        "issn": [
          "1471-2180"
        ], 
        "name": "BMC Microbiology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "keywords": [
      "otitis media", 
      "common respiratory pathogens", 
      "chronic otitis media", 
      "Streptococcus pneumoniae clinical isolates", 
      "middle ear mucosa", 
      "pneumoniae clinical isolates", 
      "major causative agent", 
      "capsule expression", 
      "respiratory infections", 
      "nasopharyngeal isolates", 
      "respiratory pathogens", 
      "tympanostomy tubes", 
      "ear mucosa", 
      "infectious process", 
      "pneumococcal strains", 
      "Streptococcus pneumoniae", 
      "clinical isolates", 
      "capsule operon", 
      "pneumococcal biofilms", 
      "causative agent", 
      "antibiotic resistance", 
      "pneumoniae", 
      "downregulation", 
      "azithromycin", 
      "cpsA gene", 
      "multiparametric analysis", 
      "treatment", 
      "pathogenic bacteria", 
      "high biofilm", 
      "DNase treatment", 
      "expression", 
      "day period", 
      "adherent growth", 
      "biopsy", 
      "isolates", 
      "BFI", 
      "mucosa", 
      "biofilm matrix", 
      "cPSA", 
      "infection", 
      "children", 
      "strains", 
      "planktonic cultures", 
      "genes", 
      "colocalization", 
      "pathogens", 
      "group", 
      "agents", 
      "biofilm biomass", 
      "biofilms", 
      "evidence", 
      "index", 
      "period", 
      "development", 
      "high BFI", 
      "biofilm development", 
      "six", 
      "resistance", 
      "hypothesis", 
      "culture", 
      "growth", 
      "bacteria", 
      "first gene", 
      "dsDNA", 
      "DNase I", 
      "biofilm growth", 
      "tube", 
      "analysis", 
      "part", 
      "extensive biofilms", 
      "medium", 
      "characterization", 
      "degradation", 
      "process", 
      "matrix", 
      "operon", 
      "complexity", 
      "structural complexity", 
      "biomass", 
      "pneumococcal biofilm formation six", 
      "biofilm formation six", 
      "formation six", 
      "greater lectin colocalization", 
      "lectin colocalization", 
      "low BFI", 
      "pneumoniae biofilm matrix", 
      "expression of cpsA", 
      "pneumococcal capsule operon", 
      "low BFI strains", 
      "BFI strains", 
      "extracellular dsDNA", 
      "greater carbohydrate-associated structural complexity", 
      "carbohydrate-associated structural complexity", 
      "capsule downregulation"
    ], 
    "name": "Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolates", 
    "pagination": "173-173", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1030255514"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2180-8-173"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18842140"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2180-8-173", 
      "https://app.dimensions.ai/details/publication/pub.1030255514"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_468.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/1471-2180-8-173"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2180-8-173'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2180-8-173'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2180-8-173'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2180-8-173'


 

This table displays all metadata directly associated to this object as RDF triples.

289 TRIPLES      22 PREDICATES      133 URIs      124 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2180-8-173 schema:about N09ca64a13def425b975a95eb8ed2661a
2 N1ad2f8a830ec44198dcc9c802905e8d3
3 N28e4c671d5eb4c3da1409877410307bf
4 N32d784e391af4bbcb9bd847b893867c4
5 N3586ab05b5bc4277912618eca255331c
6 N4deacae48a084bf1b7ff71079f439e29
7 N6959e1eccf2641adbebabc2afa3f4165
8 N81d820f5556548929b1aa8bf8ec352c3
9 N8275dd9a5e204e9580de4344812e0c29
10 N9c32ae4ac19444a185176fbebc9587a5
11 Nd043b963824d4135a32382516ce661d3
12 Ne0613ee89d324cbcb88a1f450c6e3c1e
13 anzsrc-for:11
14 anzsrc-for:1108
15 schema:author N7054bf845b574e00961b51ec604bd0cf
16 schema:citation sg:pub.10.1038/nrmicro821
17 schema:datePublished 2008-10-08
18 schema:datePublishedReg 2008-10-08
19 schema:description BACKGROUND: Streptococcus pneumoniae is a common respiratory pathogen and a major causative agent of respiratory infections, including otitis media (OM). Pneumococcal biofilms have been demonstrated on biopsies of the middle ear mucosa in children receiving tympanostomy tubes, supporting the hypothesis that chronic OM may involve biofilm development by pathogenic bacteria as part of the infectious process. To better understand pneumococcal biofilm formation six low-passage encapsulated nasopharyngeal isolates of S. pneumoniae were assessed over a six-eight day period in vitro. RESULTS: Multiparametric analysis divided the strains into two groups. Those with a high biofilm forming index (BFI) were structurally complex, exhibited greater lectin colocalization and were more resistant to azithromycin. Those with a low BFI developed less extensive biofilms and were more susceptible to azithromycin. dsDNA was present in the S. pneumoniae biofilm matrix in all strains and treatment with DNase I significantly reduced biofilm biomass. Since capsule expression has been hypothesized to be associated with decreased biofilm development, we also examined expression of cpsA, the first gene in the pneumococcal capsule operon. Interestingly, cpsA was downregulated in biofilms in both high and low BFI strains. CONCLUSION: All pneumococcal strains developed biofilms that exhibited extracellular dsDNA in the biofilm matrix, however strains with a high BFI correlated with greater carbohydrate-associated structural complexity and antibiotic resistance. Furthermore, all strains of S. pneumoniae showed downregulation of the cpsA gene during biofilm growth compared to planktonic culture, regardless of BFI ranking, suggesting downregulation of capsule expression occurs generally during adherent growth.
20 schema:genre article
21 schema:inLanguage en
22 schema:isAccessibleForFree true
23 schema:isPartOf N7803b1ac45b94e0bbb26142e189c7ab9
24 Nbd8f279f954b40689de1a5c5ba9bb754
25 sg:journal.1024253
26 schema:keywords BFI
27 BFI strains
28 DNase I
29 DNase treatment
30 Streptococcus pneumoniae
31 Streptococcus pneumoniae clinical isolates
32 adherent growth
33 agents
34 analysis
35 antibiotic resistance
36 azithromycin
37 bacteria
38 biofilm biomass
39 biofilm development
40 biofilm formation six
41 biofilm growth
42 biofilm matrix
43 biofilms
44 biomass
45 biopsy
46 cPSA
47 capsule downregulation
48 capsule expression
49 capsule operon
50 carbohydrate-associated structural complexity
51 causative agent
52 characterization
53 children
54 chronic otitis media
55 clinical isolates
56 colocalization
57 common respiratory pathogens
58 complexity
59 cpsA gene
60 culture
61 day period
62 degradation
63 development
64 downregulation
65 dsDNA
66 ear mucosa
67 evidence
68 expression
69 expression of cpsA
70 extensive biofilms
71 extracellular dsDNA
72 first gene
73 formation six
74 genes
75 greater carbohydrate-associated structural complexity
76 greater lectin colocalization
77 group
78 growth
79 high BFI
80 high biofilm
81 hypothesis
82 index
83 infection
84 infectious process
85 isolates
86 lectin colocalization
87 low BFI
88 low BFI strains
89 major causative agent
90 matrix
91 medium
92 middle ear mucosa
93 mucosa
94 multiparametric analysis
95 nasopharyngeal isolates
96 operon
97 otitis media
98 part
99 pathogenic bacteria
100 pathogens
101 period
102 planktonic cultures
103 pneumococcal biofilm formation six
104 pneumococcal biofilms
105 pneumococcal capsule operon
106 pneumococcal strains
107 pneumoniae
108 pneumoniae biofilm matrix
109 pneumoniae clinical isolates
110 process
111 resistance
112 respiratory infections
113 respiratory pathogens
114 six
115 strains
116 structural complexity
117 treatment
118 tube
119 tympanostomy tubes
120 schema:name Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolates
121 schema:pagination 173-173
122 schema:productId N29ece225250b4d279d2ad32dffe9a04f
123 N3e92e33d866b40b9baf49ebfa08c0c68
124 N701873885dc34cc7a3fa2f3b62539cdb
125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030255514
126 https://doi.org/10.1186/1471-2180-8-173
127 schema:sdDatePublished 2022-01-01T18:18
128 schema:sdLicense https://scigraph.springernature.com/explorer/license/
129 schema:sdPublisher N764c8450f5bf46d1b8f3265eedd8200a
130 schema:url https://doi.org/10.1186/1471-2180-8-173
131 sgo:license sg:explorer/license/
132 sgo:sdDataset articles
133 rdf:type schema:ScholarlyArticle
134 N059521b3aeb14f789f34da11c4c9233d rdf:first sg:person.01263223327.40
135 rdf:rest N3fb133fa242e41d58a2ac3216fb7ddff
136 N075a1789012a40bcbbe362819545f592 rdf:first Neab2ffd71daa407b9f0992a99764af6d
137 rdf:rest N88f421bc5e444aa393cac3e612d44dad
138 N09ca64a13def425b975a95eb8ed2661a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Gene Expression Profiling
140 rdf:type schema:DefinedTerm
141 N1ad2f8a830ec44198dcc9c802905e8d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Colony Count, Microbial
143 rdf:type schema:DefinedTerm
144 N28e4c671d5eb4c3da1409877410307bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Nasopharynx
146 rdf:type schema:DefinedTerm
147 N29ece225250b4d279d2ad32dffe9a04f schema:name doi
148 schema:value 10.1186/1471-2180-8-173
149 rdf:type schema:PropertyValue
150 N32d784e391af4bbcb9bd847b893867c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Child
152 rdf:type schema:DefinedTerm
153 N3586ab05b5bc4277912618eca255331c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name DNA, Bacterial
155 rdf:type schema:DefinedTerm
156 N3e92e33d866b40b9baf49ebfa08c0c68 schema:name dimensions_id
157 schema:value pub.1030255514
158 rdf:type schema:PropertyValue
159 N3fb133fa242e41d58a2ac3216fb7ddff rdf:first sg:person.01367600263.44
160 rdf:rest N9d49596df81d482db6ded2082fa4b3ff
161 N4deacae48a084bf1b7ff71079f439e29 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Azithromycin
163 rdf:type schema:DefinedTerm
164 N6959e1eccf2641adbebabc2afa3f4165 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Bacterial Proteins
166 rdf:type schema:DefinedTerm
167 N701873885dc34cc7a3fa2f3b62539cdb schema:name pubmed_id
168 schema:value 18842140
169 rdf:type schema:PropertyValue
170 N7054bf845b574e00961b51ec604bd0cf rdf:first sg:person.0607041661.06
171 rdf:rest N059521b3aeb14f789f34da11c4c9233d
172 N764c8450f5bf46d1b8f3265eedd8200a schema:name Springer Nature - SN SciGraph project
173 rdf:type schema:Organization
174 N7803b1ac45b94e0bbb26142e189c7ab9 schema:volumeNumber 8
175 rdf:type schema:PublicationVolume
176 N81d820f5556548929b1aa8bf8ec352c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Streptococcus pneumoniae
178 rdf:type schema:DefinedTerm
179 N8275dd9a5e204e9580de4344812e0c29 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Biofilms
181 rdf:type schema:DefinedTerm
182 N88f421bc5e444aa393cac3e612d44dad rdf:first sg:person.010625263442.13
183 rdf:rest N9455363ddc094090859208acd8283272
184 N9455363ddc094090859208acd8283272 rdf:first sg:person.01316722264.07
185 rdf:rest Nbbcbfee0e3c747dda49bd89c71f5285b
186 N9c32ae4ac19444a185176fbebc9587a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name Humans
188 rdf:type schema:DefinedTerm
189 N9ce79be90e1f46a3b86db74ee7abf75a rdf:first sg:person.0644477423.28
190 rdf:rest Nf725773bc75d4c66bcd00fce009f0949
191 N9d49596df81d482db6ded2082fa4b3ff rdf:first sg:person.01274441357.07
192 rdf:rest N9ce79be90e1f46a3b86db74ee7abf75a
193 Nbbcbfee0e3c747dda49bd89c71f5285b rdf:first sg:person.01324252605.57
194 rdf:rest Nef20653b6ea1483b87be40105d1db6a5
195 Nbd8f279f954b40689de1a5c5ba9bb754 schema:issueNumber 1
196 rdf:type schema:PublicationIssue
197 Nd043b963824d4135a32382516ce661d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name Anti-Bacterial Agents
199 rdf:type schema:DefinedTerm
200 Ne0613ee89d324cbcb88a1f450c6e3c1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
201 schema:name Deoxyribonuclease I
202 rdf:type schema:DefinedTerm
203 Neab2ffd71daa407b9f0992a99764af6d schema:affiliation grid-institutes:grid.280673.8
204 schema:familyName Johnson
205 schema:givenName Candice
206 rdf:type schema:Person
207 Nef20653b6ea1483b87be40105d1db6a5 rdf:first sg:person.01055120221.02
208 rdf:rest rdf:nil
209 Nf725773bc75d4c66bcd00fce009f0949 rdf:first sg:person.0733063361.13
210 rdf:rest N075a1789012a40bcbbe362819545f592
211 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
212 schema:name Medical and Health Sciences
213 rdf:type schema:DefinedTerm
214 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
215 schema:name Medical Microbiology
216 rdf:type schema:DefinedTerm
217 sg:grant.2488072 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2180-8-173
218 rdf:type schema:MonetaryGrant
219 sg:grant.2488387 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2180-8-173
220 rdf:type schema:MonetaryGrant
221 sg:grant.2488646 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2180-8-173
222 rdf:type schema:MonetaryGrant
223 sg:journal.1024253 schema:issn 1471-2180
224 schema:name BMC Microbiology
225 schema:publisher Springer Nature
226 rdf:type schema:Periodical
227 sg:person.01055120221.02 schema:affiliation grid-institutes:grid.166341.7
228 schema:familyName Post
229 schema:givenName J Christopher
230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055120221.02
231 rdf:type schema:Person
232 sg:person.010625263442.13 schema:affiliation grid-institutes:grid.166341.7
233 schema:familyName Hu
234 schema:givenName Fen Ze
235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010625263442.13
236 rdf:type schema:Person
237 sg:person.01263223327.40 schema:affiliation grid-institutes:grid.280673.8
238 schema:familyName Nistico
239 schema:givenName Laura
240 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263223327.40
241 rdf:type schema:Person
242 sg:person.01274441357.07 schema:affiliation grid-institutes:grid.280673.8
243 schema:familyName Dice
244 schema:givenName Bethany
245 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274441357.07
246 rdf:type schema:Person
247 sg:person.01316722264.07 schema:affiliation grid-institutes:grid.166341.7
248 schema:familyName Stoodley
249 schema:givenName Paul
250 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316722264.07
251 rdf:type schema:Person
252 sg:person.01324252605.57 schema:affiliation grid-institutes:grid.166341.7
253 schema:familyName Ehrlich
254 schema:givenName Garth D
255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324252605.57
256 rdf:type schema:Person
257 sg:person.01367600263.44 schema:affiliation grid-institutes:grid.280673.8
258 schema:familyName Sambanthamoorthy
259 schema:givenName Karthik
260 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367600263.44
261 rdf:type schema:Person
262 sg:person.0607041661.06 schema:affiliation grid-institutes:grid.166341.7
263 schema:familyName Hall-Stoodley
264 schema:givenName Luanne
265 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607041661.06
266 rdf:type schema:Person
267 sg:person.0644477423.28 schema:affiliation grid-institutes:grid.280673.8
268 schema:familyName Nguyen
269 schema:givenName Duc
270 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0644477423.28
271 rdf:type schema:Person
272 sg:person.0733063361.13 schema:affiliation grid-institutes:None
273 schema:familyName Mershon
274 schema:givenName William J
275 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733063361.13
276 rdf:type schema:Person
277 sg:pub.10.1038/nrmicro821 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039651347
278 https://doi.org/10.1038/nrmicro821
279 rdf:type schema:CreativeWork
280 grid-institutes:None schema:alternateName Tescan USA Inc, 508 Thomson Park Drive, Cranberry Township, PA 16066, USA
281 schema:name Tescan USA Inc, 508 Thomson Park Drive, Cranberry Township, PA 16066, USA
282 rdf:type schema:Organization
283 grid-institutes:grid.166341.7 schema:alternateName Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA
284 schema:name Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA
285 Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA 15212, USA
286 rdf:type schema:Organization
287 grid-institutes:grid.280673.8 schema:alternateName Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA
288 schema:name Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA
289 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...