Use of flow cytometry for the adhesion analysis of Streptococcus pyogenes mutant strains to epithelial cells: investigation of the possible ... View Full Text


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Article Info

DATE

2006-02-24

AUTHORS

Jukka Hytönen, Sauli Haataja, Jukka Finne

ABSTRACT

BackgroundFlow cytometry based adherence assay is a potentially powerful but little used method in the study of bacterial binding to host structures. We have previously characterized a glycoprotein-binding activity in Streptococcus pyogenes called 'strepadhesin' binding to thyroglobulin, submaxillar mucin, fetuin and asialofetuin. We have identified surface-associated pullulanase (PulA) and cysteine protease (SpeB) as carriers of strepadhesin activity. In the present paper, we investigated the use of flow cytometry as a method to study the binding of Rgg, SpeB and PulA knock-out strains to cultured human epithelial cells.ResultsStreptococcal mutants were readily labelled with CFDA-SE and their binding to epithelial cells could be effectively studied by flow cytometry. A strain deficient in Rgg expression showed increased binding to the analyzed epithelial cell lines of various origin. Inactivation of SpeB had no effect on the adhesion, while PulA knock-out strains displayed decreased binding to the cell lines.ConclusionThese results suggest that the flow cytometric assay is a valuable tool in the analysis of S. pyogenes adherence to host cells. It appears to be an efficient and sensitive tool for the characterization of interactions between the bacteria and the host at the molecular level. The results also suggest a role for Rgg regulated surface molecules, like PulA, in the adhesion of S. pyogenes to host cells. More... »

PAGES

18

References to SciGraph publications

Journal

TITLE

BMC Microbiology

ISSUE

1

VOLUME

6

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1471-2180-6-18

    DOI

    http://dx.doi.org/10.1186/1471-2180-6-18

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1050675818

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16504124


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