A multi locus variable number of tandem repeat analysis (MLVA) scheme for Streptococcus agalactiae genotyping View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-07-27

AUTHORS

Eve Haguenoer, Gaelle Baty, Christine Pourcel, Marie-Frédérique Lartigue, Anne-Sophie Domelier, Agnès Rosenau, Roland Quentin, Laurent Mereghetti, Philippe Lanotte

ABSTRACT

BackgroundMultilocus sequence typing (MLST) is currently the reference method for genotyping Streptococcus agalactiae strains, the leading cause of infectious disease in newborns and a major cause of disease in immunocompromised children and adults. We describe here a genotyping method based on multiple locus variable number of tandem repeat (VNTR) analysis (MLVA) applied to a population of S. agalactiae strains of various origins characterized by MLST and serotyping.ResultsWe studied a collection of 186 strains isolated from humans and cattle and three reference strains (A909, NEM316 and 2603 V/R). Among 34 VNTRs, 6 polymorphic VNTRs loci were selected for use in genotyping of the bacterial population. The MLVA profile consists of a series of allele numbers, corresponding to the number of repeats at each VNTR locus. 98 MLVA genotypes were obtained compared to 51 sequences types generated by MLST. The MLVA scheme generated clusters which corresponded well to the main clonal complexes obtained by MLST. However it provided a higher discriminatory power. The diversity index obtained with MLVA was 0.960 compared to 0.881 with MLST for this population of strains.ConclusionsThe MLVA scheme proposed here is a rapid, cheap and easy genotyping method generating results suitable for exchange and comparison between different laboratories and for the epidemiologic surveillance of S. agalactiae and analyses of outbreaks. More... »

PAGES

171

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2180-11-171

DOI

http://dx.doi.org/10.1186/1471-2180-11-171

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028171741

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21794143


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32 schema:description BackgroundMultilocus sequence typing (MLST) is currently the reference method for genotyping Streptococcus agalactiae strains, the leading cause of infectious disease in newborns and a major cause of disease in immunocompromised children and adults. We describe here a genotyping method based on multiple locus variable number of tandem repeat (VNTR) analysis (MLVA) applied to a population of S. agalactiae strains of various origins characterized by MLST and serotyping.ResultsWe studied a collection of 186 strains isolated from humans and cattle and three reference strains (A909, NEM316 and 2603 V/R). Among 34 VNTRs, 6 polymorphic VNTRs loci were selected for use in genotyping of the bacterial population. The MLVA profile consists of a series of allele numbers, corresponding to the number of repeats at each VNTR locus. 98 MLVA genotypes were obtained compared to 51 sequences types generated by MLST. The MLVA scheme generated clusters which corresponded well to the main clonal complexes obtained by MLST. However it provided a higher discriminatory power. The diversity index obtained with MLVA was 0.960 compared to 0.881 with MLST for this population of strains.ConclusionsThe MLVA scheme proposed here is a rapid, cheap and easy genotyping method generating results suitable for exchange and comparison between different laboratories and for the epidemiologic surveillance of S. agalactiae and analyses of outbreaks.
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40 MLVA
41 MLVA genotypes
42 MLVA profiles
43 MLVA scheme
44 ResultsWe
45 S. agalactiae
46 Streptococcus agalactiae
47 VNTR
48 VNTR loci
49 adults
50 agalactiae
51 allele number
52 analysis
53 analysis of outbreaks
54 analysis scheme
55 bacterial populations
56 cattle
57 cause
58 children
59 clonal complexes
60 clusters
61 collection
62 comparison
63 complexes
64 different laboratories
65 discriminatory power
66 disease
67 diversity index
68 epidemiologic surveillance
69 exchange
70 genotypes
71 genotyping
72 genotyping method
73 high discriminatory power
74 humans
75 immunocompromised children
76 index
77 infectious diseases
78 laboratory
79 loci
80 main clonal complexes
81 major cause
82 method
83 multi-locus variable number
84 multiple-locus variable number
85 newborns
86 number
87 number of repeats
88 origin
89 outbreak
90 polymorphic VNTR loci
91 population
92 population of strains
93 power
94 profile
95 reference
96 reference method
97 repeat analysis
98 repeats
99 results
100 scheme
101 sequence types
102 sequence typing
103 series
104 serotyping
105 strains
106 surveillance
107 tandem repeat analysis
108 tandem repeat analysis (MLVA) scheme
109 types
110 typing
111 use
112 variable number
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