In situ transduction of stromal cells and thymocytes upon intrathymic injection of lentiviral vectors View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2004-12

AUTHORS

Gilles Marodon, David Klatzmann

ABSTRACT

BACKGROUND: The thymus is the primary site for T-cell development and induction of self-tolerance. Previous approaches towards manipulation of T-cell differentiation have used intrathymic injection of antigens, as proteins, cells or adenoviruses, leading to transient expression of the foreign protein. Lentiviral vectors, due to their unique ability to integrate into the genome of quiescent cells, may be best suited for long-term expression of a transgene in the thymus. RESULTS: Young adult mice were injected in the thymus with lentiviral vectors expressing eGFP or the hemaglutinin of the Influenza virus under the control of the ubiquitous phospho glycerate kinase promoter. Thymi were examined 5 to 90 days thereafter directly under a UV-light microscope and by flow cytometry. Intrathymic injection of lentiviral vectors predominantly results in infection of stromal cells that could be detected for at least 3 months. Importantly, hemaglutinin expression by thymic stromal cells mediated negative selection of thymocytes expressing the cognate T-cell receptor. In addition and despite the low multiplicity of infection, transduced thymocytes were also detected, even 30 days after injection. CONCLUSIONS: Our results demonstrate that intrathymic delivery of a lentiviral vector is an efficient means for stable expression of a foreign gene in the thymus. This new method of gene delivery may prove useful for induction of tolerance to a specific antigen and for gene therapy of severe combined immunodeficiencies. More... »

PAGES

18

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2172-5-18

DOI

http://dx.doi.org/10.1186/1471-2172-5-18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027183939

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15318949


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Clonal Deletion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Synthetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Green Fluorescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hemagglutinin Glycoproteins, Influenza Virus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immune Tolerance", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Injections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lentivirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred C57BL", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Transgenic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoglycerate Kinase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Antigen, T-Cell", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stromal Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Cell Antigen Receptor Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thymus Gland", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transgenes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "UPMC/CNRS UMR7087, 83 Bd de l'H\u00f4pital, B\u00e2t CERVI, 75651, PARIS cedex 13, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Marodon", 
        "givenName": "Gilles", 
        "id": "sg:person.01025273641.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025273641.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "UPMC/CNRS UMR7087, 83 Bd de l'H\u00f4pital, B\u00e2t CERVI, 75651, PARIS cedex 13, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Klatzmann", 
        "givenName": "David", 
        "id": "sg:person.0774460013.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774460013.91"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.97.1.274", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001215996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(94)90368-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002708889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0952-7915(99)00069-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004903559"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.immunol.22.012703.104614", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005581783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/npg.els.0000526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008042249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/76095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021315149", 
          "https://doi.org/10.1038/76095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/76095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021315149", 
          "https://doi.org/10.1038/76095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0952-7915(96)80089-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024276546"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci119085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024563205"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci119085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024563205"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90167-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024698434"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.189.11.1735", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024867252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.180.1.25", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030822225"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1521-2254(200009/10)2:5<308::aid-jgm131>3.0.co;2-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031491669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.182.2.357", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034412707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-7613(00)80515-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041327795"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/mthe.2002.0630", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044269335"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2002-02-0578", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045438339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1197-314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045816864", 
          "https://doi.org/10.1038/ng1197-314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood.v100.3.813", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053076037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2119056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062522825"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.272.5259.263", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062552729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci17098", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063416249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.170.1.279", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1075207405"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083104000", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2004-12", 
    "datePublishedReg": "2004-12-01", 
    "description": "BACKGROUND: The thymus is the primary site for T-cell development and induction of self-tolerance. Previous approaches towards manipulation of T-cell differentiation have used intrathymic injection of antigens, as proteins, cells or adenoviruses, leading to transient expression of the foreign protein. Lentiviral vectors, due to their unique ability to integrate into the genome of quiescent cells, may be best suited for long-term expression of a transgene in the thymus.\nRESULTS: Young adult mice were injected in the thymus with lentiviral vectors expressing eGFP or the hemaglutinin of the Influenza virus under the control of the ubiquitous phospho glycerate kinase promoter. Thymi were examined 5 to 90 days thereafter directly under a UV-light microscope and by flow cytometry. Intrathymic injection of lentiviral vectors predominantly results in infection of stromal cells that could be detected for at least 3 months. Importantly, hemaglutinin expression by thymic stromal cells mediated negative selection of thymocytes expressing the cognate T-cell receptor. In addition and despite the low multiplicity of infection, transduced thymocytes were also detected, even 30 days after injection.\nCONCLUSIONS: Our results demonstrate that intrathymic delivery of a lentiviral vector is an efficient means for stable expression of a foreign gene in the thymus. This new method of gene delivery may prove useful for induction of tolerance to a specific antigen and for gene therapy of severe combined immunodeficiencies.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1471-2172-5-18", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1024252", 
        "issn": [
          "1471-2172"
        ], 
        "name": "BMC Immunology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "5"
      }
    ], 
    "name": "In situ transduction of stromal cells and thymocytes upon intrathymic injection of lentiviral vectors", 
    "pagination": "18", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "817e2e0b1aad9487dfa31e73d715d6c081715d9a94a0137c30680e529eb8bf05"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "15318949"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100966980"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2172-5-18"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1027183939"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2172-5-18", 
      "https://app.dimensions.ai/details/publication/pub.1027183939"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:50", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000505.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1471-2172-5-18"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2172-5-18'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2172-5-18'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2172-5-18'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2172-5-18'


 

This table displays all metadata directly associated to this object as RDF triples.

238 TRIPLES      21 PREDICATES      75 URIs      44 LITERALS      32 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2172-5-18 schema:about N097a0d0888b94bcaa7782c6727a0539e
2 N303a72047c894ee0a6e7e48ce194759b
3 N31e9ece94dd847acb8ec5655ea7adb4e
4 N32abdc33d3ae42069a09798388c16637
5 N46e68e74bc95440ea81df58b74d15ebd
6 N475b7d3267314673bca82215e7e5ec69
7 N4859476c403e4760b02ed52efb1f08d0
8 N58e669933c6f45b59887f96d7261d05f
9 N5b3be3f425434bd1a6ab7988bf292c71
10 N669a3fce04d0489b956902b48d643806
11 N6a9525a6180b4508bd31e4b997979538
12 N7006aec922414b5e9d5bf0446cb359ac
13 N7f98de508b1b41b890e6c9566282d535
14 N882bfba24532412e9997ad64341c1a1a
15 Na0cbb90b398a49d393e4c3abebd68bb1
16 Na8be8ed91a6d415394bf4f15a195ef18
17 Nbce8bc9d505c42b7bec610739044932c
18 Nbd653e79aa464227928ada22ed0307a2
19 Nc514c51e9bad47e1b42320317cb7864a
20 Nd8fbcc2d998a4496a4a3b2835c576b34
21 Ne01c625c7f3e422fbcbebb20177e31e2
22 Ne344f0fc334d4a5bba21d738164ab7b7
23 Nea174a44fa1448caac04a6ea5d3737ac
24 anzsrc-for:11
25 anzsrc-for:1107
26 schema:author Nfb2637f0eccc4b97b026dac8bf4a7c94
27 schema:citation sg:pub.10.1038/76095
28 sg:pub.10.1038/ng1197-314
29 https://app.dimensions.ai/details/publication/pub.1083104000
30 https://doi.org/10.1002/1521-2254(200009/10)2:5<308::aid-jgm131>3.0.co;2-3
31 https://doi.org/10.1006/mthe.2002.0630
32 https://doi.org/10.1016/0092-8674(93)90167-o
33 https://doi.org/10.1016/0092-8674(94)90368-9
34 https://doi.org/10.1016/s0952-7915(96)80089-6
35 https://doi.org/10.1016/s0952-7915(99)00069-2
36 https://doi.org/10.1016/s1074-7613(00)80515-4
37 https://doi.org/10.1038/npg.els.0000526
38 https://doi.org/10.1073/pnas.97.1.274
39 https://doi.org/10.1084/jem.180.1.25
40 https://doi.org/10.1084/jem.182.2.357
41 https://doi.org/10.1084/jem.189.11.1735
42 https://doi.org/10.1126/science.2119056
43 https://doi.org/10.1126/science.272.5259.263
44 https://doi.org/10.1146/annurev.immunol.22.012703.104614
45 https://doi.org/10.1172/jci119085
46 https://doi.org/10.1172/jci17098
47 https://doi.org/10.1182/blood-2002-02-0578
48 https://doi.org/10.1182/blood.v100.3.813
49 https://doi.org/10.4049/jimmunol.170.1.279
50 schema:datePublished 2004-12
51 schema:datePublishedReg 2004-12-01
52 schema:description BACKGROUND: The thymus is the primary site for T-cell development and induction of self-tolerance. Previous approaches towards manipulation of T-cell differentiation have used intrathymic injection of antigens, as proteins, cells or adenoviruses, leading to transient expression of the foreign protein. Lentiviral vectors, due to their unique ability to integrate into the genome of quiescent cells, may be best suited for long-term expression of a transgene in the thymus. RESULTS: Young adult mice were injected in the thymus with lentiviral vectors expressing eGFP or the hemaglutinin of the Influenza virus under the control of the ubiquitous phospho glycerate kinase promoter. Thymi were examined 5 to 90 days thereafter directly under a UV-light microscope and by flow cytometry. Intrathymic injection of lentiviral vectors predominantly results in infection of stromal cells that could be detected for at least 3 months. Importantly, hemaglutinin expression by thymic stromal cells mediated negative selection of thymocytes expressing the cognate T-cell receptor. In addition and despite the low multiplicity of infection, transduced thymocytes were also detected, even 30 days after injection. CONCLUSIONS: Our results demonstrate that intrathymic delivery of a lentiviral vector is an efficient means for stable expression of a foreign gene in the thymus. This new method of gene delivery may prove useful for induction of tolerance to a specific antigen and for gene therapy of severe combined immunodeficiencies.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree true
56 schema:isPartOf N50982e04b5a643e09c01ffff934f588c
57 Nae718bc68c6e4586bffc272d9160e4e4
58 sg:journal.1024252
59 schema:name In situ transduction of stromal cells and thymocytes upon intrathymic injection of lentiviral vectors
60 schema:pagination 18
61 schema:productId N303011d9da5f4723b420b14b75501c0b
62 N761bf5af75fd47b98917b3318977112b
63 Na83e653ca35e461793276a5575b2cf22
64 Nb44f27cf51dc4f749a8c9fe3475dca9c
65 Nb86fa8a2d8d044438ee3b59f153c0b95
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027183939
67 https://doi.org/10.1186/1471-2172-5-18
68 schema:sdDatePublished 2019-04-10T15:50
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher Na731bbb3b29448c6a877f76e9a772276
71 schema:url http://link.springer.com/10.1186%2F1471-2172-5-18
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N097a0d0888b94bcaa7782c6727a0539e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Immune Tolerance
77 rdf:type schema:DefinedTerm
78 N303011d9da5f4723b420b14b75501c0b schema:name doi
79 schema:value 10.1186/1471-2172-5-18
80 rdf:type schema:PropertyValue
81 N303a72047c894ee0a6e7e48ce194759b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Promoter Regions, Genetic
83 rdf:type schema:DefinedTerm
84 N31e9ece94dd847acb8ec5655ea7adb4e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Transgenes
86 rdf:type schema:DefinedTerm
87 N32abdc33d3ae42069a09798388c16637 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Mice, Transgenic
89 rdf:type schema:DefinedTerm
90 N39faf9ed35164fb299236ba86411d817 schema:name UPMC/CNRS UMR7087, 83 Bd de l'Hôpital, Bât CERVI, 75651, PARIS cedex 13, France
91 rdf:type schema:Organization
92 N46e68e74bc95440ea81df58b74d15ebd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Mice
94 rdf:type schema:DefinedTerm
95 N475b7d3267314673bca82215e7e5ec69 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name T-Cell Antigen Receptor Specificity
97 rdf:type schema:DefinedTerm
98 N4859476c403e4760b02ed52efb1f08d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Lentivirus
100 rdf:type schema:DefinedTerm
101 N50982e04b5a643e09c01ffff934f588c schema:issueNumber 1
102 rdf:type schema:PublicationIssue
103 N58e669933c6f45b59887f96d7261d05f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Recombinant Fusion Proteins
105 rdf:type schema:DefinedTerm
106 N5b3be3f425434bd1a6ab7988bf292c71 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Hemagglutinin Glycoproteins, Influenza Virus
108 rdf:type schema:DefinedTerm
109 N669a3fce04d0489b956902b48d643806 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Stromal Cells
111 rdf:type schema:DefinedTerm
112 N6a9525a6180b4508bd31e4b997979538 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Phosphoglycerate Kinase
114 rdf:type schema:DefinedTerm
115 N7006aec922414b5e9d5bf0446cb359ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Green Fluorescent Proteins
117 rdf:type schema:DefinedTerm
118 N761bf5af75fd47b98917b3318977112b schema:name pubmed_id
119 schema:value 15318949
120 rdf:type schema:PropertyValue
121 N776480037f464140851aa437f42665f9 schema:name UPMC/CNRS UMR7087, 83 Bd de l'Hôpital, Bât CERVI, 75651, PARIS cedex 13, France
122 rdf:type schema:Organization
123 N7f98de508b1b41b890e6c9566282d535 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Clonal Deletion
125 rdf:type schema:DefinedTerm
126 N882bfba24532412e9997ad64341c1a1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Animals
128 rdf:type schema:DefinedTerm
129 Na0cbb90b398a49d393e4c3abebd68bb1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name T-Lymphocytes
131 rdf:type schema:DefinedTerm
132 Na731bbb3b29448c6a877f76e9a772276 schema:name Springer Nature - SN SciGraph project
133 rdf:type schema:Organization
134 Na83e653ca35e461793276a5575b2cf22 schema:name readcube_id
135 schema:value 817e2e0b1aad9487dfa31e73d715d6c081715d9a94a0137c30680e529eb8bf05
136 rdf:type schema:PropertyValue
137 Na8be8ed91a6d415394bf4f15a195ef18 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Mice, Inbred C57BL
139 rdf:type schema:DefinedTerm
140 Nae718bc68c6e4586bffc272d9160e4e4 schema:volumeNumber 5
141 rdf:type schema:PublicationVolume
142 Nb12d2e1f85954acc96f6c773c9dd7952 rdf:first sg:person.0774460013.91
143 rdf:rest rdf:nil
144 Nb44f27cf51dc4f749a8c9fe3475dca9c schema:name nlm_unique_id
145 schema:value 100966980
146 rdf:type schema:PropertyValue
147 Nb86fa8a2d8d044438ee3b59f153c0b95 schema:name dimensions_id
148 schema:value pub.1027183939
149 rdf:type schema:PropertyValue
150 Nbce8bc9d505c42b7bec610739044932c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Genes, Synthetic
152 rdf:type schema:DefinedTerm
153 Nbd653e79aa464227928ada22ed0307a2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Genetic Vectors
155 rdf:type schema:DefinedTerm
156 Nc514c51e9bad47e1b42320317cb7864a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Genes, Reporter
158 rdf:type schema:DefinedTerm
159 Nd8fbcc2d998a4496a4a3b2835c576b34 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Transduction, Genetic
161 rdf:type schema:DefinedTerm
162 Ne01c625c7f3e422fbcbebb20177e31e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Thymus Gland
164 rdf:type schema:DefinedTerm
165 Ne344f0fc334d4a5bba21d738164ab7b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Injections
167 rdf:type schema:DefinedTerm
168 Nea174a44fa1448caac04a6ea5d3737ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Receptors, Antigen, T-Cell
170 rdf:type schema:DefinedTerm
171 Nfb2637f0eccc4b97b026dac8bf4a7c94 rdf:first sg:person.01025273641.62
172 rdf:rest Nb12d2e1f85954acc96f6c773c9dd7952
173 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
174 schema:name Medical and Health Sciences
175 rdf:type schema:DefinedTerm
176 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
177 schema:name Immunology
178 rdf:type schema:DefinedTerm
179 sg:journal.1024252 schema:issn 1471-2172
180 schema:name BMC Immunology
181 rdf:type schema:Periodical
182 sg:person.01025273641.62 schema:affiliation N776480037f464140851aa437f42665f9
183 schema:familyName Marodon
184 schema:givenName Gilles
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025273641.62
186 rdf:type schema:Person
187 sg:person.0774460013.91 schema:affiliation N39faf9ed35164fb299236ba86411d817
188 schema:familyName Klatzmann
189 schema:givenName David
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774460013.91
191 rdf:type schema:Person
192 sg:pub.10.1038/76095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021315149
193 https://doi.org/10.1038/76095
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/ng1197-314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045816864
196 https://doi.org/10.1038/ng1197-314
197 rdf:type schema:CreativeWork
198 https://app.dimensions.ai/details/publication/pub.1083104000 schema:CreativeWork
199 https://doi.org/10.1002/1521-2254(200009/10)2:5<308::aid-jgm131>3.0.co;2-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031491669
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1006/mthe.2002.0630 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044269335
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/0092-8674(93)90167-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1024698434
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/0092-8674(94)90368-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002708889
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/s0952-7915(96)80089-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024276546
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/s0952-7915(99)00069-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004903559
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/s1074-7613(00)80515-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041327795
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1038/npg.els.0000526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008042249
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1073/pnas.97.1.274 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001215996
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1084/jem.180.1.25 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030822225
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1084/jem.182.2.357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034412707
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1084/jem.189.11.1735 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024867252
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1126/science.2119056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062522825
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1126/science.272.5259.263 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062552729
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1146/annurev.immunol.22.012703.104614 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005581783
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1172/jci119085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024563205
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1172/jci17098 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063416249
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1182/blood-2002-02-0578 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045438339
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1182/blood.v100.3.813 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053076037
236 rdf:type schema:CreativeWork
237 https://doi.org/10.4049/jimmunol.170.1.279 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075207405
238 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...