Genome-wide transcriptome analysis reveals that a pleiotropic antibiotic regulator, AfsS, modulates nutritional stress response in Streptomyces coelicolor A3(2) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-01-29

AUTHORS

Wei Lian, Karthik P Jayapal, Salim Charaniya, Sarika Mehra, Frank Glod, Yun-Seung Kyung, David H Sherman, Wei-Shou Hu

ABSTRACT

BACKGROUND: A small "sigma-like" protein, AfsS, pleiotropically regulates antibiotic biosynthesis in Streptomyces coelicolor. Overexpression of afsS in S. coelicolor and certain related species causes antibiotic stimulatory effects in the host organism. Although recent studies have uncovered some of the upstream events activating this gene, the mechanisms through which this signal is relayed downstream leading to the eventual induction of antibiotic pathways remain unclear. RESULTS: In this study, we employed whole-genome DNA microarrays and quantitative PCRs to examine the transcriptome of an afsS disruption mutant that is completely deficient in the production of actinorhodin, a major S. coelicolor antibiotic. The production of undecylprodigiosin, another prominent antibiotic, was, however, perturbed only marginally in the mutant. Principal component analysis of temporal gene expression profiles identified two major gene classes each exhibiting a distinct coordinate differential expression pattern. Surprisingly, nearly 70% of the >117 differentially expressed genes were conspicuously associated with nutrient starvation response, particularly those of phosphate, nitrogen and sulfate. Furthermore, expression profiles of some transcriptional regulators including at least two sigma factors were perturbed in the mutant. In almost every case, the effect of afsS disruption was not observed until the onset of stationary phase. CONCLUSION: Our data suggests a comprehensive role for S. coelicolor AfsS as a master regulator of both antibiotic synthesis and nutritional stress response, reminiscent of alternative sigma factors found in several bacteria. More... »

PAGES

56-56

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2164-9-56

DOI

http://dx.doi.org/10.1186/1471-2164-9-56

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038415246

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18230178


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38 schema:description BACKGROUND: A small "sigma-like" protein, AfsS, pleiotropically regulates antibiotic biosynthesis in Streptomyces coelicolor. Overexpression of afsS in S. coelicolor and certain related species causes antibiotic stimulatory effects in the host organism. Although recent studies have uncovered some of the upstream events activating this gene, the mechanisms through which this signal is relayed downstream leading to the eventual induction of antibiotic pathways remain unclear. RESULTS: In this study, we employed whole-genome DNA microarrays and quantitative PCRs to examine the transcriptome of an afsS disruption mutant that is completely deficient in the production of actinorhodin, a major S. coelicolor antibiotic. The production of undecylprodigiosin, another prominent antibiotic, was, however, perturbed only marginally in the mutant. Principal component analysis of temporal gene expression profiles identified two major gene classes each exhibiting a distinct coordinate differential expression pattern. Surprisingly, nearly 70% of the >117 differentially expressed genes were conspicuously associated with nutrient starvation response, particularly those of phosphate, nitrogen and sulfate. Furthermore, expression profiles of some transcriptional regulators including at least two sigma factors were perturbed in the mutant. In almost every case, the effect of afsS disruption was not observed until the onset of stationary phase. CONCLUSION: Our data suggests a comprehensive role for S. coelicolor AfsS as a master regulator of both antibiotic synthesis and nutritional stress response, reminiscent of alternative sigma factors found in several bacteria.
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45 schema:keywords AFS
46 DNA microarrays
47 Overexpression of afsS
48 PCR
49 Recent studies
50 Streptomyces coelicolor
51 actinorhodin
52 afsS disruption
53 afsS disruption mutant
54 alternative sigma factor
55 analysis
56 antibiotic biosynthesis
57 antibiotic pathways
58 antibiotic regulator
59 antibiotic stimulatory effects
60 antibiotic synthesis
61 antibiotics
62 bacteria
63 biosynthesis
64 cases
65 class
66 coelicolor
67 coelicolor AfsS
68 component analysis
69 comprehensive role
70 coordinate differential expression pattern
71 data
72 differential expression patterns
73 disruption
74 disruption mutant
75 distinct coordinate differential expression pattern
76 effect
77 events
78 eventual induction
79 expression patterns
80 expression profiles
81 factors
82 gene classes
83 gene expression profiles
84 genes
85 genome-wide transcriptome analysis
86 host organism
87 induction
88 major gene classes
89 master regulator
90 mechanism
91 microarrays
92 mutants
93 nitrogen
94 nutrient starvation response
95 nutritional stress response
96 onset
97 organisms
98 overexpression
99 pathway
100 patterns
101 phase
102 phosphate
103 pleiotropic antibiotic regulator
104 principal component analysis
105 production
106 production of actinorhodin
107 production of undecylprodigiosin
108 profile
109 prominent antibiotics
110 protein
111 quantitative PCR
112 regulator
113 response
114 role
115 sigma factor
116 signals
117 species
118 starvation response
119 stationary phase
120 stimulatory effect
121 stress response
122 study
123 sulfate
124 synthesis
125 temporal gene expression profiles
126 transcriptional regulators
127 transcriptome
128 transcriptome analysis
129 undecylprodigiosin
130 upstream events
131 whole-genome DNA microarrays
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