Design and validation of a supragenome array for determination of the genomic content of Haemophilus influenzae isolates View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-07-17

AUTHORS

Rory A Eutsey, N Luisa Hiller, Joshua P Earl, Benjamin A Janto, Margaret E Dahlgren, Azad Ahmed, Evan Powell, Matthew P Schultz, Janet R Gilsdorf, Lixin Zhang, Arnold Smith, Timothy F Murphy, Sanjay Sethi, Kai Shen, J Christopher Post, Fen Z Hu, Garth D Ehrlich

ABSTRACT

BackgroundHaemophilus influenzae colonizes the human nasopharynx as a commensal, and is etiologically associated with numerous opportunistic infections of the airway; it is also less commonly associated with invasive disease. Clinical isolates of H. influenzae display extensive genomic diversity and plasticity. The development of strategies to successfully prevent, diagnose and treat H. influenzae infections depends on tools to ascertain the gene content of individual isolates.ResultsWe describe and validate a Haemophilus influenzae supragenome hybridization (SGH) array that can be used to characterize the full genic complement of any strain within the species, as well as strains from several highly related species. The array contains 31,307 probes that collectively cover essentially all alleles of the 2890 gene clusters identified from the whole genome sequencing of 24 clinical H. influenzae strains. The finite supragenome model predicts that these data include greater than 85% of all non-rare genes (where rare genes are defined as those present in less than 10% of sequenced strains). The veracity of the array was tested by comparing the whole genome sequences of eight strains with their hybridization data obtained using the supragenome array. The array predictions were correct and reproducible for ~ 98% of the gene content of all of the sequenced strains. This technology was then applied to an investigation of the gene content of 193 geographically and clinically diverse H. influenzae clinical strains. These strains came from multiple locations from five different continents and Papua New Guinea and include isolates from: the middle ears of persons with otitis media and otorrhea; lung aspirates and sputum samples from pneumonia and COPD patients, blood specimens from patients with sepsis; cerebrospinal fluid from patients with meningitis, as well as from pharyngeal specimens from healthy persons.ConclusionsThese analyses provided the most comprehensive and detailed genomic/phylogenetic look at this species to date, and identified a subset of highly divergent strains that form a separate lineage within the species. This array provides a cost-effective and high-throughput tool to determine the gene content of any H. influenzae isolate or lineage. Furthermore, the method for probe selection can be applied to any species, given a group of available whole genome sequences. More... »

PAGES

484

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2164-14-484

DOI

http://dx.doi.org/10.1186/1471-2164-14-484

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037361173

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23865594


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genomics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Haemophilus influenzae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Annotation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Hybridization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oligonucleotide Array Sequence Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.413621.3", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Eutsey", 
        "givenName": "Rory A", 
        "id": "sg:person.0576023665.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576023665.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.147455.6", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
            "Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hiller", 
        "givenName": "N Luisa", 
        "id": "sg:person.01303646644.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303646644.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.413621.3", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Earl", 
        "givenName": "Joshua P", 
        "id": "sg:person.015030316477.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015030316477.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.166341.7", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
            "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Janto", 
        "givenName": "Benjamin A", 
        "id": "sg:person.01332700436.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332700436.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.413621.3", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dahlgren", 
        "givenName": "Margaret E", 
        "id": "sg:person.01241374544.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241374544.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.413621.3", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ahmed", 
        "givenName": "Azad", 
        "id": "sg:person.0754221125.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754221125.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.413621.3", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Powell", 
        "givenName": "Evan", 
        "id": "sg:person.01321124525.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321124525.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.413621.3", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schultz", 
        "givenName": "Matthew P", 
        "id": "sg:person.01150551534.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150551534.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pediatrics and Communicable Diseases, University of Michigan School of Public Health, Ann Arbor, MC, USA", 
          "id": "http://www.grid.ac/institutes/grid.214458.e", 
          "name": [
            "Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MC, USA", 
            "Department of Pediatrics and Communicable Diseases, University of Michigan School of Public Health, Ann Arbor, MC, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gilsdorf", 
        "givenName": "Janet R", 
        "id": "sg:person.01326125654.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326125654.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MC, USA", 
          "id": "http://www.grid.ac/institutes/grid.214458.e", 
          "name": [
            "Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MC, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Lixin", 
        "id": "sg:person.0672726613.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672726613.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Childhood Infections, Seattle Children\u2019s Hospital Research Institute, Seattle, WA, USA", 
          "id": "http://www.grid.ac/institutes/grid.240741.4", 
          "name": [
            "Center for Childhood Infections, Seattle Children\u2019s Hospital Research Institute, Seattle, WA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Smith", 
        "givenName": "Arnold", 
        "id": "sg:person.01104353520.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104353520.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA", 
          "id": "http://www.grid.ac/institutes/grid.273335.3", 
          "name": [
            "Department of Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murphy", 
        "givenName": "Timothy F", 
        "id": "sg:person.015563027057.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015563027057.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA", 
          "id": "http://www.grid.ac/institutes/grid.273335.3", 
          "name": [
            "Department of Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sethi", 
        "givenName": "Sanjay", 
        "id": "sg:person.01206176723.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206176723.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.166341.7", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
            "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA", 
            "Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shen", 
        "givenName": "Kai", 
        "id": "sg:person.01350047321.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350047321.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.166341.7", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
            "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA", 
            "Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Post", 
        "givenName": "J Christopher", 
        "id": "sg:person.01055120221.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055120221.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.166341.7", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
            "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA", 
            "Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hu", 
        "givenName": "Fen Z", 
        "id": "sg:person.010625263442.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010625263442.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.166341.7", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA", 
            "Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA", 
            "Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ehrlich", 
        "givenName": "Garth D", 
        "id": "sg:person.01324252605.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324252605.57"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/1471-2164-12-187", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002886020", 
          "https://doi.org/10.1186/1471-2164-12-187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2180-8-173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030255514", 
          "https://doi.org/10.1186/1471-2180-8-173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2012-13-7-r64", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010707384", 
          "https://doi.org/10.1186/gb-2012-13-7-r64"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-12-70", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036426417", 
          "https://doi.org/10.1186/1471-2164-12-70"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2180-7-56", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052986553", 
          "https://doi.org/10.1186/1471-2180-7-56"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-9-75", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013431920", 
          "https://doi.org/10.1186/1471-2164-9-75"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2007-8-6-r103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047555346", 
          "https://doi.org/10.1186/gb-2007-8-6-r103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2008-9-6-225", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041268307", 
          "https://doi.org/10.1186/gb-2008-9-6-225"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-13-373", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004314788", 
          "https://doi.org/10.1186/1471-2164-13-373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2010-11-10-r107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034216670", 
          "https://doi.org/10.1186/gb-2010-11-10-r107"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-07-17", 
    "datePublishedReg": "2013-07-17", 
    "description": "BackgroundHaemophilus influenzae colonizes the human nasopharynx as a commensal, and is etiologically associated with numerous opportunistic infections of the airway; it is also less commonly associated with invasive disease. Clinical isolates of H. influenzae display extensive genomic diversity and plasticity. The development of strategies to successfully prevent, diagnose and treat H. influenzae infections depends on tools to ascertain the gene content of individual isolates.ResultsWe describe and validate a Haemophilus influenzae supragenome hybridization (SGH) array that can be used to characterize the full genic complement of any strain within the species, as well as strains from several highly related species. The array contains 31,307 probes that collectively cover essentially all alleles of the 2890 gene clusters identified from the whole genome sequencing of 24 clinical H. influenzae strains. The finite supragenome model predicts that these data include greater than 85% of all non-rare genes (where rare genes are defined as those present in less than 10% of sequenced strains). The veracity of the array was tested by comparing the whole genome sequences of eight strains with their hybridization data obtained using the supragenome array. The array predictions were correct and reproducible for ~ 98% of the gene content of all of the sequenced strains. This technology was then applied to an investigation of the gene content of 193 geographically and clinically diverse H. influenzae clinical strains. These strains came from multiple locations from five different continents and Papua New Guinea and include isolates from: the middle ears of persons with otitis media and otorrhea; lung aspirates and sputum samples from pneumonia and COPD patients, blood specimens from patients with sepsis; cerebrospinal fluid from patients with meningitis, as well as from pharyngeal specimens from healthy persons.ConclusionsThese analyses provided the most comprehensive and detailed genomic/phylogenetic look at this species to date, and identified a subset of highly divergent strains that form a separate lineage within the species. This array provides a cost-effective and high-throughput tool to determine the gene content of any H. influenzae isolate or lineage. Furthermore, the method for probe selection can be applied to any species, given a group of available whole genome sequences.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/1471-2164-14-484", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2488072", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2449980", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2458520", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1023790", 
        "issn": [
          "1471-2164"
        ], 
        "name": "BMC Genomics", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "14"
      }
    ], 
    "keywords": [
      "H. influenzae", 
      "Haemophilus influenzae", 
      "H. influenzae infections", 
      "H. influenzae isolates", 
      "numerous opportunistic infections", 
      "clinical H. influenzae", 
      "COPD patients", 
      "influenzae infection", 
      "lung aspirates", 
      "influenzae isolates", 
      "opportunistic infections", 
      "otitis media", 
      "pharyngeal specimens", 
      "invasive disease", 
      "sputum samples", 
      "healthy persons", 
      "blood specimens", 
      "cerebrospinal fluid", 
      "clinical isolates", 
      "middle ear", 
      "human nasopharynx", 
      "influenzae", 
      "patients", 
      "clinical strains", 
      "ConclusionsThese analyses", 
      "gene content", 
      "whole genome sequences", 
      "development of strategies", 
      "whole-genome sequencing", 
      "infection", 
      "genome sequence", 
      "divergent strains", 
      "isolates", 
      "available whole genome sequences", 
      "extensive genomic diversity", 
      "sepsis", 
      "meningitis", 
      "pneumonia", 
      "airway", 
      "nasopharynx", 
      "aspirates", 
      "disease", 
      "hybridization arrays", 
      "persons", 
      "ResultsWe", 
      "high-throughput tool", 
      "genome sequencing", 
      "gene cluster", 
      "genomic diversity", 
      "genomic content", 
      "ear", 
      "separate lineages", 
      "strains", 
      "individual isolates", 
      "hybridization data", 
      "commensal", 
      "specimens", 
      "Papua New Guinea", 
      "species", 
      "group", 
      "lineages", 
      "subset", 
      "alleles", 
      "plasticity", 
      "data", 
      "sequencing", 
      "different continents", 
      "array predictions", 
      "genes", 
      "New Guinea", 
      "date", 
      "probe selection", 
      "complement", 
      "sequence", 
      "fluid", 
      "tool", 
      "samples", 
      "diversity", 
      "development", 
      "strategies", 
      "multiple locations", 
      "content", 
      "investigation", 
      "analysis", 
      "validation", 
      "Guinea", 
      "location", 
      "array", 
      "selection", 
      "probe", 
      "method", 
      "medium", 
      "model", 
      "clusters", 
      "continent", 
      "determination", 
      "design", 
      "prediction", 
      "look", 
      "technology", 
      "veracity"
    ], 
    "name": "Design and validation of a supragenome array for determination of the genomic content of Haemophilus influenzae isolates", 
    "pagination": "484", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037361173"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2164-14-484"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "23865594"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2164-14-484", 
      "https://app.dimensions.ai/details/publication/pub.1037361173"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T17:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_611.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/1471-2164-14-484"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2164-14-484'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2164-14-484'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2164-14-484'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2164-14-484'


 

This table displays all metadata directly associated to this object as RDF triples.

368 TRIPLES      21 PREDICATES      143 URIs      125 LITERALS      14 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2164-14-484 schema:about N28f510307967451689ce18d26d7fcbdc
2 N3681241a5f594e7e967ef2453734aecd
3 N41590a19e4e94f61a5558bd7a3315df9
4 N55d085ee78d046c598dfb29f1a5ebc1d
5 N64c0d4822c1a423fa8fa35175cde7d40
6 N7cd408b043404530a3012d6057a1cc1b
7 Nc0556bb8db214fe99c5b12b5f5aad26c
8 anzsrc-for:06
9 anzsrc-for:0604
10 schema:author Ncddfb0043c584884a41abbaecc72ea1e
11 schema:citation sg:pub.10.1186/1471-2164-12-187
12 sg:pub.10.1186/1471-2164-12-70
13 sg:pub.10.1186/1471-2164-13-373
14 sg:pub.10.1186/1471-2164-9-75
15 sg:pub.10.1186/1471-2180-7-56
16 sg:pub.10.1186/1471-2180-8-173
17 sg:pub.10.1186/gb-2007-8-6-r103
18 sg:pub.10.1186/gb-2008-9-6-225
19 sg:pub.10.1186/gb-2010-11-10-r107
20 sg:pub.10.1186/gb-2012-13-7-r64
21 schema:datePublished 2013-07-17
22 schema:datePublishedReg 2013-07-17
23 schema:description BackgroundHaemophilus influenzae colonizes the human nasopharynx as a commensal, and is etiologically associated with numerous opportunistic infections of the airway; it is also less commonly associated with invasive disease. Clinical isolates of H. influenzae display extensive genomic diversity and plasticity. The development of strategies to successfully prevent, diagnose and treat H. influenzae infections depends on tools to ascertain the gene content of individual isolates.ResultsWe describe and validate a Haemophilus influenzae supragenome hybridization (SGH) array that can be used to characterize the full genic complement of any strain within the species, as well as strains from several highly related species. The array contains 31,307 probes that collectively cover essentially all alleles of the 2890 gene clusters identified from the whole genome sequencing of 24 clinical H. influenzae strains. The finite supragenome model predicts that these data include greater than 85% of all non-rare genes (where rare genes are defined as those present in less than 10% of sequenced strains). The veracity of the array was tested by comparing the whole genome sequences of eight strains with their hybridization data obtained using the supragenome array. The array predictions were correct and reproducible for ~ 98% of the gene content of all of the sequenced strains. This technology was then applied to an investigation of the gene content of 193 geographically and clinically diverse H. influenzae clinical strains. These strains came from multiple locations from five different continents and Papua New Guinea and include isolates from: the middle ears of persons with otitis media and otorrhea; lung aspirates and sputum samples from pneumonia and COPD patients, blood specimens from patients with sepsis; cerebrospinal fluid from patients with meningitis, as well as from pharyngeal specimens from healthy persons.ConclusionsThese analyses provided the most comprehensive and detailed genomic/phylogenetic look at this species to date, and identified a subset of highly divergent strains that form a separate lineage within the species. This array provides a cost-effective and high-throughput tool to determine the gene content of any H. influenzae isolate or lineage. Furthermore, the method for probe selection can be applied to any species, given a group of available whole genome sequences.
24 schema:genre article
25 schema:isAccessibleForFree true
26 schema:isPartOf N58bf24a68f9d47a593c72a31e32908a9
27 N8e67f15b47554684a068fec45f8c26c4
28 sg:journal.1023790
29 schema:keywords COPD patients
30 ConclusionsThese analyses
31 Guinea
32 H. influenzae
33 H. influenzae infections
34 H. influenzae isolates
35 Haemophilus influenzae
36 New Guinea
37 Papua New Guinea
38 ResultsWe
39 airway
40 alleles
41 analysis
42 array
43 array predictions
44 aspirates
45 available whole genome sequences
46 blood specimens
47 cerebrospinal fluid
48 clinical H. influenzae
49 clinical isolates
50 clinical strains
51 clusters
52 commensal
53 complement
54 content
55 continent
56 data
57 date
58 design
59 determination
60 development
61 development of strategies
62 different continents
63 disease
64 divergent strains
65 diversity
66 ear
67 extensive genomic diversity
68 fluid
69 gene cluster
70 gene content
71 genes
72 genome sequence
73 genome sequencing
74 genomic content
75 genomic diversity
76 group
77 healthy persons
78 high-throughput tool
79 human nasopharynx
80 hybridization arrays
81 hybridization data
82 individual isolates
83 infection
84 influenzae
85 influenzae infection
86 influenzae isolates
87 invasive disease
88 investigation
89 isolates
90 lineages
91 location
92 look
93 lung aspirates
94 medium
95 meningitis
96 method
97 middle ear
98 model
99 multiple locations
100 nasopharynx
101 numerous opportunistic infections
102 opportunistic infections
103 otitis media
104 patients
105 persons
106 pharyngeal specimens
107 plasticity
108 pneumonia
109 prediction
110 probe
111 probe selection
112 samples
113 selection
114 separate lineages
115 sepsis
116 sequence
117 sequencing
118 species
119 specimens
120 sputum samples
121 strains
122 strategies
123 subset
124 technology
125 tool
126 validation
127 veracity
128 whole genome sequences
129 whole-genome sequencing
130 schema:name Design and validation of a supragenome array for determination of the genomic content of Haemophilus influenzae isolates
131 schema:pagination 484
132 schema:productId N0f51253a6dd24781be3d2b2e6baa6b39
133 N2cf6483032fe44fc955815e1487138ed
134 N4180dd833aa4403ebcd7f302cfd6134f
135 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037361173
136 https://doi.org/10.1186/1471-2164-14-484
137 schema:sdDatePublished 2022-08-04T17:00
138 schema:sdLicense https://scigraph.springernature.com/explorer/license/
139 schema:sdPublisher N7d510e8c2d5340138b7c61db33d2f82b
140 schema:url https://doi.org/10.1186/1471-2164-14-484
141 sgo:license sg:explorer/license/
142 sgo:sdDataset articles
143 rdf:type schema:ScholarlyArticle
144 N0f51253a6dd24781be3d2b2e6baa6b39 schema:name pubmed_id
145 schema:value 23865594
146 rdf:type schema:PropertyValue
147 N28f510307967451689ce18d26d7fcbdc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Haemophilus influenzae
149 rdf:type schema:DefinedTerm
150 N2cf6483032fe44fc955815e1487138ed schema:name doi
151 schema:value 10.1186/1471-2164-14-484
152 rdf:type schema:PropertyValue
153 N2ef801b539f14c16872a3a0bc85e9c1d rdf:first sg:person.01332700436.44
154 rdf:rest N7332dd0923f54f048c2af2f94562fdcb
155 N3681241a5f594e7e967ef2453734aecd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Nucleic Acid Hybridization
157 rdf:type schema:DefinedTerm
158 N3c0d3ea7ff354ba59b68db809bc1458c rdf:first sg:person.01104353520.48
159 rdf:rest Na6629b623b5b49f9ba29f775f954d4c4
160 N3c5060bd2a4b4e7b9a19bade8ef68ed7 rdf:first sg:person.01326125654.44
161 rdf:rest Ne41d440ba18146c79c75e0be652f9a10
162 N41590a19e4e94f61a5558bd7a3315df9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Sequence Analysis
164 rdf:type schema:DefinedTerm
165 N4180dd833aa4403ebcd7f302cfd6134f schema:name dimensions_id
166 schema:value pub.1037361173
167 rdf:type schema:PropertyValue
168 N55d085ee78d046c598dfb29f1a5ebc1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Genomics
170 rdf:type schema:DefinedTerm
171 N58bf24a68f9d47a593c72a31e32908a9 schema:issueNumber 1
172 rdf:type schema:PublicationIssue
173 N5d5dbc5527294ea490f9ebf29ab5d7a3 rdf:first sg:person.01324252605.57
174 rdf:rest rdf:nil
175 N64c0d4822c1a423fa8fa35175cde7d40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Oligonucleotide Array Sequence Analysis
177 rdf:type schema:DefinedTerm
178 N71bf4a8225c448c6a90dd27e1ebc5b49 rdf:first sg:person.01321124525.00
179 rdf:rest Nd4c24c8672684ff2a8ef94b9b5b1a0d2
180 N7332dd0923f54f048c2af2f94562fdcb rdf:first sg:person.01241374544.00
181 rdf:rest N79301691b77048e4aede476f762c3748
182 N79301691b77048e4aede476f762c3748 rdf:first sg:person.0754221125.53
183 rdf:rest N71bf4a8225c448c6a90dd27e1ebc5b49
184 N7cd408b043404530a3012d6057a1cc1b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Genes, Bacterial
186 rdf:type schema:DefinedTerm
187 N7d510e8c2d5340138b7c61db33d2f82b schema:name Springer Nature - SN SciGraph project
188 rdf:type schema:Organization
189 N8e67f15b47554684a068fec45f8c26c4 schema:volumeNumber 14
190 rdf:type schema:PublicationVolume
191 Na6629b623b5b49f9ba29f775f954d4c4 rdf:first sg:person.015563027057.11
192 rdf:rest Nd12ebad3419f44eb800a3a9a1556ccfc
193 Naacf2eed6fd248b48d7cfe15f889b6c5 rdf:first sg:person.01055120221.02
194 rdf:rest Nf55b8e4a521b44a0af7e9705e94c5bcb
195 Naf8b2893aefd4ec59e17b507716626f2 rdf:first sg:person.01350047321.57
196 rdf:rest Naacf2eed6fd248b48d7cfe15f889b6c5
197 Nc0556bb8db214fe99c5b12b5f5aad26c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name Molecular Sequence Annotation
199 rdf:type schema:DefinedTerm
200 Nc0e003b5f8554c7c8609eeac6936d0dc rdf:first sg:person.01303646644.41
201 rdf:rest Ne410ac568b5b4ddbb631b40772ecaa02
202 Ncddfb0043c584884a41abbaecc72ea1e rdf:first sg:person.0576023665.62
203 rdf:rest Nc0e003b5f8554c7c8609eeac6936d0dc
204 Nd12ebad3419f44eb800a3a9a1556ccfc rdf:first sg:person.01206176723.07
205 rdf:rest Naf8b2893aefd4ec59e17b507716626f2
206 Nd4c24c8672684ff2a8ef94b9b5b1a0d2 rdf:first sg:person.01150551534.22
207 rdf:rest N3c5060bd2a4b4e7b9a19bade8ef68ed7
208 Ne410ac568b5b4ddbb631b40772ecaa02 rdf:first sg:person.015030316477.18
209 rdf:rest N2ef801b539f14c16872a3a0bc85e9c1d
210 Ne41d440ba18146c79c75e0be652f9a10 rdf:first sg:person.0672726613.97
211 rdf:rest N3c0d3ea7ff354ba59b68db809bc1458c
212 Nf55b8e4a521b44a0af7e9705e94c5bcb rdf:first sg:person.010625263442.13
213 rdf:rest N5d5dbc5527294ea490f9ebf29ab5d7a3
214 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
215 schema:name Biological Sciences
216 rdf:type schema:DefinedTerm
217 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
218 schema:name Genetics
219 rdf:type schema:DefinedTerm
220 sg:grant.2449980 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2164-14-484
221 rdf:type schema:MonetaryGrant
222 sg:grant.2458520 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2164-14-484
223 rdf:type schema:MonetaryGrant
224 sg:grant.2488072 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2164-14-484
225 rdf:type schema:MonetaryGrant
226 sg:journal.1023790 schema:issn 1471-2164
227 schema:name BMC Genomics
228 schema:publisher Springer Nature
229 rdf:type schema:Periodical
230 sg:person.01055120221.02 schema:affiliation grid-institutes:grid.166341.7
231 schema:familyName Post
232 schema:givenName J Christopher
233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055120221.02
234 rdf:type schema:Person
235 sg:person.010625263442.13 schema:affiliation grid-institutes:grid.166341.7
236 schema:familyName Hu
237 schema:givenName Fen Z
238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010625263442.13
239 rdf:type schema:Person
240 sg:person.01104353520.48 schema:affiliation grid-institutes:grid.240741.4
241 schema:familyName Smith
242 schema:givenName Arnold
243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104353520.48
244 rdf:type schema:Person
245 sg:person.01150551534.22 schema:affiliation grid-institutes:grid.413621.3
246 schema:familyName Schultz
247 schema:givenName Matthew P
248 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150551534.22
249 rdf:type schema:Person
250 sg:person.01206176723.07 schema:affiliation grid-institutes:grid.273335.3
251 schema:familyName Sethi
252 schema:givenName Sanjay
253 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206176723.07
254 rdf:type schema:Person
255 sg:person.01241374544.00 schema:affiliation grid-institutes:grid.413621.3
256 schema:familyName Dahlgren
257 schema:givenName Margaret E
258 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241374544.00
259 rdf:type schema:Person
260 sg:person.01303646644.41 schema:affiliation grid-institutes:grid.147455.6
261 schema:familyName Hiller
262 schema:givenName N Luisa
263 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303646644.41
264 rdf:type schema:Person
265 sg:person.01321124525.00 schema:affiliation grid-institutes:grid.413621.3
266 schema:familyName Powell
267 schema:givenName Evan
268 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321124525.00
269 rdf:type schema:Person
270 sg:person.01324252605.57 schema:affiliation grid-institutes:grid.166341.7
271 schema:familyName Ehrlich
272 schema:givenName Garth D
273 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324252605.57
274 rdf:type schema:Person
275 sg:person.01326125654.44 schema:affiliation grid-institutes:grid.214458.e
276 schema:familyName Gilsdorf
277 schema:givenName Janet R
278 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326125654.44
279 rdf:type schema:Person
280 sg:person.01332700436.44 schema:affiliation grid-institutes:grid.166341.7
281 schema:familyName Janto
282 schema:givenName Benjamin A
283 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332700436.44
284 rdf:type schema:Person
285 sg:person.01350047321.57 schema:affiliation grid-institutes:grid.166341.7
286 schema:familyName Shen
287 schema:givenName Kai
288 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350047321.57
289 rdf:type schema:Person
290 sg:person.015030316477.18 schema:affiliation grid-institutes:grid.413621.3
291 schema:familyName Earl
292 schema:givenName Joshua P
293 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015030316477.18
294 rdf:type schema:Person
295 sg:person.015563027057.11 schema:affiliation grid-institutes:grid.273335.3
296 schema:familyName Murphy
297 schema:givenName Timothy F
298 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015563027057.11
299 rdf:type schema:Person
300 sg:person.0576023665.62 schema:affiliation grid-institutes:grid.413621.3
301 schema:familyName Eutsey
302 schema:givenName Rory A
303 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576023665.62
304 rdf:type schema:Person
305 sg:person.0672726613.97 schema:affiliation grid-institutes:grid.214458.e
306 schema:familyName Zhang
307 schema:givenName Lixin
308 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672726613.97
309 rdf:type schema:Person
310 sg:person.0754221125.53 schema:affiliation grid-institutes:grid.413621.3
311 schema:familyName Ahmed
312 schema:givenName Azad
313 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754221125.53
314 rdf:type schema:Person
315 sg:pub.10.1186/1471-2164-12-187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002886020
316 https://doi.org/10.1186/1471-2164-12-187
317 rdf:type schema:CreativeWork
318 sg:pub.10.1186/1471-2164-12-70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036426417
319 https://doi.org/10.1186/1471-2164-12-70
320 rdf:type schema:CreativeWork
321 sg:pub.10.1186/1471-2164-13-373 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004314788
322 https://doi.org/10.1186/1471-2164-13-373
323 rdf:type schema:CreativeWork
324 sg:pub.10.1186/1471-2164-9-75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013431920
325 https://doi.org/10.1186/1471-2164-9-75
326 rdf:type schema:CreativeWork
327 sg:pub.10.1186/1471-2180-7-56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052986553
328 https://doi.org/10.1186/1471-2180-7-56
329 rdf:type schema:CreativeWork
330 sg:pub.10.1186/1471-2180-8-173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030255514
331 https://doi.org/10.1186/1471-2180-8-173
332 rdf:type schema:CreativeWork
333 sg:pub.10.1186/gb-2007-8-6-r103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047555346
334 https://doi.org/10.1186/gb-2007-8-6-r103
335 rdf:type schema:CreativeWork
336 sg:pub.10.1186/gb-2008-9-6-225 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041268307
337 https://doi.org/10.1186/gb-2008-9-6-225
338 rdf:type schema:CreativeWork
339 sg:pub.10.1186/gb-2010-11-10-r107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034216670
340 https://doi.org/10.1186/gb-2010-11-10-r107
341 rdf:type schema:CreativeWork
342 sg:pub.10.1186/gb-2012-13-7-r64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010707384
343 https://doi.org/10.1186/gb-2012-13-7-r64
344 rdf:type schema:CreativeWork
345 grid-institutes:grid.147455.6 schema:alternateName Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
346 schema:name Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA
347 Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
348 rdf:type schema:Organization
349 grid-institutes:grid.166341.7 schema:alternateName Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA
350 Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA
351 schema:name Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA
352 Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA
353 Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, PA, USA
354 rdf:type schema:Organization
355 grid-institutes:grid.214458.e schema:alternateName Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MC, USA
356 Department of Pediatrics and Communicable Diseases, University of Michigan School of Public Health, Ann Arbor, MC, USA
357 schema:name Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MC, USA
358 Department of Pediatrics and Communicable Diseases, University of Michigan School of Public Health, Ann Arbor, MC, USA
359 rdf:type schema:Organization
360 grid-institutes:grid.240741.4 schema:alternateName Center for Childhood Infections, Seattle Children’s Hospital Research Institute, Seattle, WA, USA
361 schema:name Center for Childhood Infections, Seattle Children’s Hospital Research Institute, Seattle, WA, USA
362 rdf:type schema:Organization
363 grid-institutes:grid.273335.3 schema:alternateName Department of Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA
364 schema:name Department of Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA
365 rdf:type schema:Organization
366 grid-institutes:grid.413621.3 schema:alternateName Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA
367 schema:name Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor, 15212, South Tower, Pittsburgh, PA, USA
368 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...