Identification of avian W-linked contigs by short-read sequencing View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-05-14

AUTHORS

Nancy Chen, Daniel W Bellott, David C Page, Andrew G Clark

ABSTRACT

BackgroundThe female-specific W chromosomes and male-specific Y chromosomes have proven difficult to assemble with whole-genome shotgun methods, creating a demand for new approaches to identify sequence contigs specific to these sex chromosomes. Here, we develop and apply a novel method for identifying sequences that are W-specific.ResultsUsing the Illumina Genome Analyzer, we generated sequence reads from a male domestic chicken (ZZ) and mapped them to the existing female (ZW) genome sequence. This method allowed us to identify segments of the female genome that are underrepresented in the male genome and are therefore likely to be female specific. We developed a Bayesian classifier to automate the calling of W-linked contigs and successfully identified more than 60 novel W-specific sequences.ConclusionsOur classifier can be applied to improve heterogametic whole-genome shotgun assemblies of the W or Y chromosome of any organism. This study greatly improves our knowledge of the W chromosome and will enhance future studies of avian sex determination and sex chromosome evolution. More... »

PAGES

183

References to SciGraph publications

  • 2002-12-31. Heterochromatic sequences in a Drosophila whole-genome shotgun assembly in GENOME BIOLOGY
  • 2003-03. Y Chromosome and Other Heterochromatic Sequences of the Drosophila Melanogaster Genome: How Far can we go? in GENETICA
  • 2004-12-09. Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution in NATURE
  • 2004-12. A genetic variation map for chicken with 2.8 million single-nucleotide polymorphisms in NATURE
  • 2009-08-26. The avian Z-linked gene DMRT1 is required for male sex determination in the chicken in NATURE
  • 2002-09. Molecular and cytological characterization of SspI-family repetitive sequence on the chicken W chromosome in CHROMOSOME RESEARCH
  • 2011-03-01. Genome structural variation discovery and genotyping in NATURE REVIEWS GENETICS
  • 2001. Chicken spindling genes on W and Z chromosomes: transcriptional expression of both genes and dynamic behavior of spindlin in interphase and mitotic cells in CHROMOSOME RESEARCH
  • 1999-06. Comparative Painting Reveals Strong Chromosome Homology Over 80 Million Years of Bird Evolution in CHROMOSOME RESEARCH
  • 2001-08. Strategies for the systematic sequencing of complex genomes in NATURE REVIEWS GENETICS
  • 2010-11-21. Limitations of next-generation genome sequence assembly in NATURE METHODS
  • 2004-01-30. Versatile and open software for comparing large genomes in GENOME BIOLOGY
  • 2010-01-13. Chimpanzee and human Y chromosomes are remarkably divergent in structure and gene content in NATURE
  • 2003-06. The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes in NATURE
  • 2009-10-15. Computational methods for discovering structural variation with next-generation sequencing in NATURE METHODS
  • 2000-04. ASW: a gene with conserved avian W-linkage and female specific expression in chick embryonic gonad in DEVELOPMENT GENES AND EVOLUTION
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1471-2164-13-183

    DOI

    http://dx.doi.org/10.1186/1471-2164-13-183

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1048193163

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22583744


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