Genome sequence of adherent-invasive Escherichia coli and comparative genomic analysis with other E. coli pathotypes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-11-25

AUTHORS

John HE Nash, Andre Villegas, Andrew M Kropinski, Renan Aguilar-Valenzuela, Paulina Konczy, Mariola Mascarenhas, Kim Ziebell, Alfredo G Torres, Mohamed A Karmali, Brian K Coombes

ABSTRACT

BackgroundAdherent and invasive Escherichia coli (AIEC) are commonly found in ileal lesions of Crohn's Disease (CD) patients, where they adhere to intestinal epithelial cells and invade into and survive in epithelial cells and macrophages, thereby gaining access to a typically restricted host niche. Colonization leads to strong inflammatory responses in the gut suggesting that AIEC could play a role in CD immunopathology. Despite extensive investigation, the genetic determinants accounting for the AIEC phenotype remain poorly defined. To address this, we present the complete genome sequence of an AIEC, revealing the genetic blueprint for this disease-associated E. coli pathotype.ResultsWe sequenced the complete genome of E. coli NRG857c (O83:H1), a clinical isolate of AIEC from the ileum of a Crohn's Disease patient. Our sequence data confirmed a phylogenetic linkage between AIEC and extraintestinal pathogenic E. coli causing urinary tract infections and neonatal meningitis. The comparison of the NRG857c AIEC genome with other pathogenic and commensal E. coli allowed for the identification of unique genetic features of the AIEC pathotype, including 41 genomic islands, and unique genes that are found only in strains exhibiting the adherent and invasive phenotype.ConclusionsUp to now, the virulence-like features associated with AIEC are detectable only phenotypically. AIEC genome sequence data will facilitate the identification of genetic determinants implicated in invasion and intracellular growth, as well as enable functional genomic studies of AIEC gene expression during health and disease. More... »

PAGES

667

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2164-11-667

DOI

http://dx.doi.org/10.1186/1471-2164-11-667

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042873783

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21108814


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32 schema:description BackgroundAdherent and invasive Escherichia coli (AIEC) are commonly found in ileal lesions of Crohn's Disease (CD) patients, where they adhere to intestinal epithelial cells and invade into and survive in epithelial cells and macrophages, thereby gaining access to a typically restricted host niche. Colonization leads to strong inflammatory responses in the gut suggesting that AIEC could play a role in CD immunopathology. Despite extensive investigation, the genetic determinants accounting for the AIEC phenotype remain poorly defined. To address this, we present the complete genome sequence of an AIEC, revealing the genetic blueprint for this disease-associated E. coli pathotype.ResultsWe sequenced the complete genome of E. coli NRG857c (O83:H1), a clinical isolate of AIEC from the ileum of a Crohn's Disease patient. Our sequence data confirmed a phylogenetic linkage between AIEC and extraintestinal pathogenic E. coli causing urinary tract infections and neonatal meningitis. The comparison of the NRG857c AIEC genome with other pathogenic and commensal E. coli allowed for the identification of unique genetic features of the AIEC pathotype, including 41 genomic islands, and unique genes that are found only in strains exhibiting the adherent and invasive phenotype.ConclusionsUp to now, the virulence-like features associated with AIEC are detectable only phenotypically. AIEC genome sequence data will facilitate the identification of genetic determinants implicated in invasion and intracellular growth, as well as enable functional genomic studies of AIEC gene expression during health and disease.
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39 AIEC pathotype
40 AIEC phenotype
41 ConclusionsUp
42 Crohn's disease patients
43 E. coli
44 E. coli pathotypes
45 Escherichia coli
46 ResultsWe
47 access
48 adherent
49 adherent-invasive Escherichia coli
50 analysis
51 blueprint
52 cells
53 clinical isolates
54 coli
55 coli pathotypes
56 colonization
57 commensal E. coli
58 comparative genomic analysis
59 comparison
60 complete genome
61 complete genome sequence
62 data
63 determinants
64 disease
65 disease patients
66 epithelial cells
67 expression
68 extensive investigation
69 extraintestinal pathogenic E. coli
70 features
71 functional genomic studies
72 gene expression
73 genes
74 genetic blueprint
75 genetic determinants
76 genetic features
77 genome
78 genome sequence
79 genome sequence data
80 genomic analysis
81 genomic islands
82 genomic studies
83 growth
84 gut
85 health
86 host niches
87 identification
88 ileal lesions
89 ileum
90 immunopathology
91 infection
92 inflammatory response
93 intestinal epithelial cells
94 intracellular growth
95 invasion
96 invasive Escherichia coli
97 invasive phenotype
98 investigation
99 islands
100 isolates
101 lesions
102 linkage
103 macrophages
104 meningitis
105 neonatal meningitis
106 niche
107 pathogenic E. coli
108 pathotypes
109 patients
110 phenotype
111 phylogenetic linkage
112 response
113 role
114 sequence
115 sequence data
116 strains
117 strong inflammatory response
118 study
119 tract infections
120 unique genes
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122 urinary tract infection
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