SPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-12

AUTHORS

Paul J Muhlrad, Jessica N Clark, Ubaydah Nasri, Nicholas G Sullivan, Craig W LaMunyon

ABSTRACT

BACKGROUND: The SPE-8 group gene products transduce the signal for spermatid activation initiated by extracellular zinc in C. elegans. Mutations in the spe-8 group genes result in hermaphrodite-derived spermatids that cannot activate to crawling spermatozoa, although spermatids from mutant males activate through a pathway induced by extracellular TRY-5 protease present in male seminal fluid. RESULTS: Here, we identify SPE-8 as a member of a large family of sperm-expressed non-receptor-like protein-tyrosine kinases. A rescuing SPE-8::GFP translational fusion reporter localizes to the plasma membrane in all spermatogenic cells from the primary spermatocyte stage through spermatids. Once spermatids become activated to spermatozoa, the reporter moves from the plasma membrane to the cytoplasm. Mutations in the spe-8 group genes spe-12, spe-19, and spe-27 disrupt localization of the reporter to the plasma membrane, while localization appears near normal in a spe-29 mutant background. CONCLUSIONS: These results suggest that the SPE-8 group proteins form a functional complex localized at the plasma membrane, and that SPE-8 is correctly positioned only when all members of the SPE-8 group are present, with the possible exception of SPE-29. Further, SPE-8 is released from the membrane when the activation signal is transduced into the spermatid. More... »

PAGES

83

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2156-15-83

DOI

http://dx.doi.org/10.1186/1471-2156-15-83

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015820844

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25022984


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caenorhabditis elegans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caenorhabditis elegans Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Membrane", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Helminth", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Exons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Tertiary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein-Tyrosine Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spermatids", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Colorado Boulder", 
          "id": "https://www.grid.ac/institutes/grid.266190.a", 
          "name": [
            "Department of Molecular and Cellular Biology, University of Arizona, 1007 Lowell St., 85721, Tucson, AZ, USA", 
            "Department of Molecular, Cellular and Developmental Biology, Currently, University of Colorado Boulder, 347 UCB, CO 80309, Boulder, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Muhlrad", 
        "givenName": "Paul J", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "California State Polytechnic University", 
          "id": "https://www.grid.ac/institutes/grid.155203.0", 
          "name": [
            "Department of Biological Science, California State Polytechnic University, 3801 W. Temple Ave, 91768, Pomona, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Clark", 
        "givenName": "Jessica N", 
        "id": "sg:person.0733641220.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733641220.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "California State Polytechnic University", 
          "id": "https://www.grid.ac/institutes/grid.155203.0", 
          "name": [
            "Department of Biological Science, California State Polytechnic University, 3801 W. Temple Ave, 91768, Pomona, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nasri", 
        "givenName": "Ubaydah", 
        "id": "sg:person.0775714212.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0775714212.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "California State Polytechnic University", 
          "id": "https://www.grid.ac/institutes/grid.155203.0", 
          "name": [
            "Department of Biological Science, California State Polytechnic University, 3801 W. Temple Ave, 91768, Pomona, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sullivan", 
        "givenName": "Nicholas G", 
        "id": "sg:person.01050067620.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050067620.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "California State Polytechnic University", 
          "id": "https://www.grid.ac/institutes/grid.155203.0", 
          "name": [
            "Department of Biological Science, California State Polytechnic University, 3801 W. Temple Ave, 91768, Pomona, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "LaMunyon", 
        "givenName": "Craig W", 
        "id": "sg:person.0756132007.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756132007.01"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1158/1541-7786.mcr-08-0117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005225308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2006.01.035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005873493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2010.5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006999469", 
          "https://doi.org/10.1038/nprot.2010.5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2010.5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006999469", 
          "https://doi.org/10.1038/nprot.2010.5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1999.3310", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007216874"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.15.8.4553", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007236613"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m001975200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009320232"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-15-168", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015992637", 
          "https://doi.org/10.1186/1471-2164-15-168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2004.07.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016040663"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-nutr-080508-141119", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016072376"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.semcdb.2014.04.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016245586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0057266", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016413222"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2443.1996.00258.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017253615"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2443.1996.00258.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017253615"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(00)00059-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019951226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.e06-08-0741", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020254143"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.00914", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021194286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.00469", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021984268"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0303-7207(99)00205-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028918693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81339-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030803090"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng.248", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031784174", 
          "https://doi.org/10.1038/ng.248"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/385595a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032796118", 
          "https://doi.org/10.1038/385595a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2407-9-366", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033054179", 
          "https://doi.org/10.1186/1471-2407-9-366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/mrd.21326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034992394"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0854.2011.01208.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035902185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.091025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036722063"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.69.1.373", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037305893"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(83)90336-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040853414"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ydbio.2005.04.036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041213872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkq979", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045239525"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0282(1998)47:3<243::aid-bip4>3.0.co;2-p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045578810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/dvdy.21789", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045630427"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mce.2013.07.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045767364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/onc.2013.277", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045877184", 
          "https://doi.org/10.1038/onc.2013.277"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1002375", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047444550"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-10-3757", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047623351"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.e11-06-0517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053339532"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gki524", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053671309"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1210795", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053718057", 
          "https://doi.org/10.1038/sj.onc.1210795"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/scisignal.2000393", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062682142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1615/critrevoncog.v9.i1.40", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068132686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2741/3961", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070916122"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074365937", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074734660", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/02324bm01", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1075036237"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075055381", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079607622", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080300025", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082926623", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-12", 
    "datePublishedReg": "2014-12-01", 
    "description": "BACKGROUND: The SPE-8 group gene products transduce the signal for spermatid activation initiated by extracellular zinc in C. elegans. Mutations in the spe-8 group genes result in hermaphrodite-derived spermatids that cannot activate to crawling spermatozoa, although spermatids from mutant males activate through a pathway induced by extracellular TRY-5 protease present in male seminal fluid.\nRESULTS: Here, we identify SPE-8 as a member of a large family of sperm-expressed non-receptor-like protein-tyrosine kinases. A rescuing SPE-8::GFP translational fusion reporter localizes to the plasma membrane in all spermatogenic cells from the primary spermatocyte stage through spermatids. Once spermatids become activated to spermatozoa, the reporter moves from the plasma membrane to the cytoplasm. Mutations in the spe-8 group genes spe-12, spe-19, and spe-27 disrupt localization of the reporter to the plasma membrane, while localization appears near normal in a spe-29 mutant background.\nCONCLUSIONS: These results suggest that the SPE-8 group proteins form a functional complex localized at the plasma membrane, and that SPE-8 is correctly positioned only when all members of the SPE-8 group are present, with the possible exception of SPE-29. Further, SPE-8 is released from the membrane when the activation signal is transduced into the spermatid.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1471-2156-15-83", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2679998", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1024251", 
        "issn": [
          "1471-2156"
        ], 
        "name": "BMC Genetics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "SPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid activation signaling pathway in C. elegans", 
    "pagination": "83", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "4b62042f5694534da82a9978159b01f68c2bc44ad584765715f8af48c2b9988b"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25022984"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100966978"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2156-15-83"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1015820844"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2156-15-83", 
      "https://app.dimensions.ai/details/publication/pub.1015820844"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000504.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1471-2156-15-83"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2156-15-83'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2156-15-83'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2156-15-83'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2156-15-83'


 

This table displays all metadata directly associated to this object as RDF triples.

307 TRIPLES      21 PREDICATES      92 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2156-15-83 schema:about N029064cfacbd497c9171458968a63b31
2 N19e1498be41e4eefa2ac63953c480b29
3 N1c71246e2f214a62898708c4674d03d1
4 N37ded79f04884e0ab2a219d48e7ef240
5 N4c509c38a53245aa94ef647353f504c0
6 N4f239e3917744cc29ed3c2860d1bfd60
7 N5972b155f038419e85664e89dcc1492c
8 N5ab2c5ad2c3d425195103690bdc9368d
9 N65b83c8c9baf4595b7c3db63813d9b43
10 N81464870867340d7b043f16ed4053284
11 N8d5c2792bf83493ead82fcfcdd0044b9
12 N9fb850522df4433ea36f3dcbfa3d8ed3
13 Nb6d0d6d805174529aee79b5933f61ef5
14 Nce5402598bb543b0961b609cf6425bf5
15 Ndf8b950388c74313a569f024dceff69e
16 Nf4942102689443aaab808b8eb184b19b
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author N659fba38551542af8c2cf62c97c86a0f
20 schema:citation sg:pub.10.1038/385595a0
21 sg:pub.10.1038/ng.248
22 sg:pub.10.1038/nprot.2010.5
23 sg:pub.10.1038/onc.2013.277
24 sg:pub.10.1038/sj.onc.1210795
25 sg:pub.10.1186/1471-2164-15-168
26 sg:pub.10.1186/1471-2407-9-366
27 https://app.dimensions.ai/details/publication/pub.1074365937
28 https://app.dimensions.ai/details/publication/pub.1074734660
29 https://app.dimensions.ai/details/publication/pub.1075055381
30 https://app.dimensions.ai/details/publication/pub.1079607622
31 https://app.dimensions.ai/details/publication/pub.1080300025
32 https://app.dimensions.ai/details/publication/pub.1082926623
33 https://doi.org/10.1002/(sici)1097-0282(1998)47:3<243::aid-bip4>3.0.co;2-p
34 https://doi.org/10.1002/dvdy.21789
35 https://doi.org/10.1002/mrd.21326
36 https://doi.org/10.1006/jmbi.1999.3310
37 https://doi.org/10.1016/0012-1606(83)90336-6
38 https://doi.org/10.1016/j.cub.2004.07.025
39 https://doi.org/10.1016/j.mce.2013.07.017
40 https://doi.org/10.1016/j.molcel.2006.01.035
41 https://doi.org/10.1016/j.semcdb.2014.04.005
42 https://doi.org/10.1016/j.ydbio.2005.04.036
43 https://doi.org/10.1016/s0092-8674(00)81339-2
44 https://doi.org/10.1016/s0303-7207(99)00205-1
45 https://doi.org/10.1016/s1097-2765(00)00059-9
46 https://doi.org/10.1046/j.1365-2443.1996.00258.x
47 https://doi.org/10.1074/jbc.m001975200
48 https://doi.org/10.1091/mbc.e06-08-0741
49 https://doi.org/10.1091/mbc.e11-06-0517
50 https://doi.org/10.1093/nar/gki524
51 https://doi.org/10.1093/nar/gkq979
52 https://doi.org/10.1111/j.1600-0854.2011.01208.x
53 https://doi.org/10.1126/scisignal.2000393
54 https://doi.org/10.1128/mcb.15.8.4553
55 https://doi.org/10.1146/annurev-nutr-080508-141119
56 https://doi.org/10.1146/annurev.biochem.69.1.373
57 https://doi.org/10.1158/0008-5472.can-10-3757
58 https://doi.org/10.1158/1541-7786.mcr-08-0117
59 https://doi.org/10.1242/dev.00914
60 https://doi.org/10.1242/dev.091025
61 https://doi.org/10.1242/jcs.00469
62 https://doi.org/10.1371/journal.pgen.1002375
63 https://doi.org/10.1371/journal.pone.0057266
64 https://doi.org/10.1615/critrevoncog.v9.i1.40
65 https://doi.org/10.2144/02324bm01
66 https://doi.org/10.2741/3961
67 schema:datePublished 2014-12
68 schema:datePublishedReg 2014-12-01
69 schema:description BACKGROUND: The SPE-8 group gene products transduce the signal for spermatid activation initiated by extracellular zinc in C. elegans. Mutations in the spe-8 group genes result in hermaphrodite-derived spermatids that cannot activate to crawling spermatozoa, although spermatids from mutant males activate through a pathway induced by extracellular TRY-5 protease present in male seminal fluid. RESULTS: Here, we identify SPE-8 as a member of a large family of sperm-expressed non-receptor-like protein-tyrosine kinases. A rescuing SPE-8::GFP translational fusion reporter localizes to the plasma membrane in all spermatogenic cells from the primary spermatocyte stage through spermatids. Once spermatids become activated to spermatozoa, the reporter moves from the plasma membrane to the cytoplasm. Mutations in the spe-8 group genes spe-12, spe-19, and spe-27 disrupt localization of the reporter to the plasma membrane, while localization appears near normal in a spe-29 mutant background. CONCLUSIONS: These results suggest that the SPE-8 group proteins form a functional complex localized at the plasma membrane, and that SPE-8 is correctly positioned only when all members of the SPE-8 group are present, with the possible exception of SPE-29. Further, SPE-8 is released from the membrane when the activation signal is transduced into the spermatid.
70 schema:genre research_article
71 schema:inLanguage en
72 schema:isAccessibleForFree true
73 schema:isPartOf N174858c464024e2689ffeea08dde066b
74 N9f07cdc055ed48f984d8199cd80f7cbf
75 sg:journal.1024251
76 schema:name SPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid activation signaling pathway in C. elegans
77 schema:pagination 83
78 schema:productId N10e608c1e2164912a1285cab166902b9
79 N57ef42ec730e4d06b6b94cac9aac6c6e
80 N62acd3e25d24474fbe61304d3ae3505d
81 Na51e52058b734c5a9c768e413554ea7b
82 Ncb93cd56f3c8407f9725fc321aca9f14
83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015820844
84 https://doi.org/10.1186/1471-2156-15-83
85 schema:sdDatePublished 2019-04-10T19:55
86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
87 schema:sdPublisher N7e4ed7c7b85e4ace8c32697ff9478bd7
88 schema:url http://link.springer.com/10.1186%2F1471-2156-15-83
89 sgo:license sg:explorer/license/
90 sgo:sdDataset articles
91 rdf:type schema:ScholarlyArticle
92 N029064cfacbd497c9171458968a63b31 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Amino Acid Sequence
94 rdf:type schema:DefinedTerm
95 N10e608c1e2164912a1285cab166902b9 schema:name dimensions_id
96 schema:value pub.1015820844
97 rdf:type schema:PropertyValue
98 N174858c464024e2689ffeea08dde066b schema:volumeNumber 15
99 rdf:type schema:PublicationVolume
100 N19e1498be41e4eefa2ac63953c480b29 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Signal Transduction
102 rdf:type schema:DefinedTerm
103 N1c71246e2f214a62898708c4674d03d1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Exons
105 rdf:type schema:DefinedTerm
106 N37ded79f04884e0ab2a219d48e7ef240 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Animals
108 rdf:type schema:DefinedTerm
109 N392e8358b7af426b9fa7244177b9f59f rdf:first sg:person.01050067620.76
110 rdf:rest Nc5b8176b892e448fb7d0790c2c44e4a3
111 N4c509c38a53245aa94ef647353f504c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Mutation
113 rdf:type schema:DefinedTerm
114 N4f239e3917744cc29ed3c2860d1bfd60 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Male
116 rdf:type schema:DefinedTerm
117 N57ef42ec730e4d06b6b94cac9aac6c6e schema:name pubmed_id
118 schema:value 25022984
119 rdf:type schema:PropertyValue
120 N5972b155f038419e85664e89dcc1492c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Protein Structure, Tertiary
122 rdf:type schema:DefinedTerm
123 N5ab2c5ad2c3d425195103690bdc9368d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Models, Molecular
125 rdf:type schema:DefinedTerm
126 N62acd3e25d24474fbe61304d3ae3505d schema:name nlm_unique_id
127 schema:value 100966978
128 rdf:type schema:PropertyValue
129 N659fba38551542af8c2cf62c97c86a0f rdf:first Nf98cfdc4dcc74b639e6013c987a79a76
130 rdf:rest Ne488942f1e76471d90541bc899d877f5
131 N65b83c8c9baf4595b7c3db63813d9b43 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Molecular Sequence Data
133 rdf:type schema:DefinedTerm
134 N7e4ed7c7b85e4ace8c32697ff9478bd7 schema:name Springer Nature - SN SciGraph project
135 rdf:type schema:Organization
136 N81464870867340d7b043f16ed4053284 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Cell Membrane
138 rdf:type schema:DefinedTerm
139 N8d5c2792bf83493ead82fcfcdd0044b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Protein-Tyrosine Kinases
141 rdf:type schema:DefinedTerm
142 N9f07cdc055ed48f984d8199cd80f7cbf schema:issueNumber 1
143 rdf:type schema:PublicationIssue
144 N9fb850522df4433ea36f3dcbfa3d8ed3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Spermatids
146 rdf:type schema:DefinedTerm
147 Na51e52058b734c5a9c768e413554ea7b schema:name readcube_id
148 schema:value 4b62042f5694534da82a9978159b01f68c2bc44ad584765715f8af48c2b9988b
149 rdf:type schema:PropertyValue
150 Nb6d0d6d805174529aee79b5933f61ef5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Caenorhabditis elegans
152 rdf:type schema:DefinedTerm
153 Nc5b8176b892e448fb7d0790c2c44e4a3 rdf:first sg:person.0756132007.01
154 rdf:rest rdf:nil
155 Ncb93cd56f3c8407f9725fc321aca9f14 schema:name doi
156 schema:value 10.1186/1471-2156-15-83
157 rdf:type schema:PropertyValue
158 Nce5402598bb543b0961b609cf6425bf5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Sequence Analysis, DNA
160 rdf:type schema:DefinedTerm
161 Ndf8b950388c74313a569f024dceff69e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name DNA, Helminth
163 rdf:type schema:DefinedTerm
164 Ne488942f1e76471d90541bc899d877f5 rdf:first sg:person.0733641220.84
165 rdf:rest Ned0b07639084482d913dca9772b65fc7
166 Ned0b07639084482d913dca9772b65fc7 rdf:first sg:person.0775714212.44
167 rdf:rest N392e8358b7af426b9fa7244177b9f59f
168 Nf4942102689443aaab808b8eb184b19b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Caenorhabditis elegans Proteins
170 rdf:type schema:DefinedTerm
171 Nf98cfdc4dcc74b639e6013c987a79a76 schema:affiliation https://www.grid.ac/institutes/grid.266190.a
172 schema:familyName Muhlrad
173 schema:givenName Paul J
174 rdf:type schema:Person
175 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
176 schema:name Biological Sciences
177 rdf:type schema:DefinedTerm
178 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
179 schema:name Biochemistry and Cell Biology
180 rdf:type schema:DefinedTerm
181 sg:grant.2679998 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2156-15-83
182 rdf:type schema:MonetaryGrant
183 sg:journal.1024251 schema:issn 1471-2156
184 schema:name BMC Genetics
185 rdf:type schema:Periodical
186 sg:person.01050067620.76 schema:affiliation https://www.grid.ac/institutes/grid.155203.0
187 schema:familyName Sullivan
188 schema:givenName Nicholas G
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050067620.76
190 rdf:type schema:Person
191 sg:person.0733641220.84 schema:affiliation https://www.grid.ac/institutes/grid.155203.0
192 schema:familyName Clark
193 schema:givenName Jessica N
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733641220.84
195 rdf:type schema:Person
196 sg:person.0756132007.01 schema:affiliation https://www.grid.ac/institutes/grid.155203.0
197 schema:familyName LaMunyon
198 schema:givenName Craig W
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756132007.01
200 rdf:type schema:Person
201 sg:person.0775714212.44 schema:affiliation https://www.grid.ac/institutes/grid.155203.0
202 schema:familyName Nasri
203 schema:givenName Ubaydah
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0775714212.44
205 rdf:type schema:Person
206 sg:pub.10.1038/385595a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032796118
207 https://doi.org/10.1038/385595a0
208 rdf:type schema:CreativeWork
209 sg:pub.10.1038/ng.248 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031784174
210 https://doi.org/10.1038/ng.248
211 rdf:type schema:CreativeWork
212 sg:pub.10.1038/nprot.2010.5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006999469
213 https://doi.org/10.1038/nprot.2010.5
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/onc.2013.277 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045877184
216 https://doi.org/10.1038/onc.2013.277
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/sj.onc.1210795 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053718057
219 https://doi.org/10.1038/sj.onc.1210795
220 rdf:type schema:CreativeWork
221 sg:pub.10.1186/1471-2164-15-168 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015992637
222 https://doi.org/10.1186/1471-2164-15-168
223 rdf:type schema:CreativeWork
224 sg:pub.10.1186/1471-2407-9-366 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033054179
225 https://doi.org/10.1186/1471-2407-9-366
226 rdf:type schema:CreativeWork
227 https://app.dimensions.ai/details/publication/pub.1074365937 schema:CreativeWork
228 https://app.dimensions.ai/details/publication/pub.1074734660 schema:CreativeWork
229 https://app.dimensions.ai/details/publication/pub.1075055381 schema:CreativeWork
230 https://app.dimensions.ai/details/publication/pub.1079607622 schema:CreativeWork
231 https://app.dimensions.ai/details/publication/pub.1080300025 schema:CreativeWork
232 https://app.dimensions.ai/details/publication/pub.1082926623 schema:CreativeWork
233 https://doi.org/10.1002/(sici)1097-0282(1998)47:3<243::aid-bip4>3.0.co;2-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1045578810
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1002/dvdy.21789 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045630427
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1002/mrd.21326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034992394
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1006/jmbi.1999.3310 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007216874
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/0012-1606(83)90336-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040853414
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/j.cub.2004.07.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016040663
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/j.mce.2013.07.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045767364
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/j.molcel.2006.01.035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005873493
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/j.semcdb.2014.04.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016245586
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1016/j.ydbio.2005.04.036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041213872
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1016/s0092-8674(00)81339-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030803090
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1016/s0303-7207(99)00205-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028918693
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1016/s1097-2765(00)00059-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019951226
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1046/j.1365-2443.1996.00258.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017253615
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1074/jbc.m001975200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009320232
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1091/mbc.e06-08-0741 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020254143
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1091/mbc.e11-06-0517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053339532
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1093/nar/gki524 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053671309
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1093/nar/gkq979 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045239525
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1111/j.1600-0854.2011.01208.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035902185
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1126/scisignal.2000393 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062682142
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1128/mcb.15.8.4553 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007236613
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1146/annurev-nutr-080508-141119 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016072376
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1146/annurev.biochem.69.1.373 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037305893
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1158/0008-5472.can-10-3757 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047623351
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1158/1541-7786.mcr-08-0117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005225308
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1242/dev.00914 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021194286
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1242/dev.091025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036722063
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1242/jcs.00469 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021984268
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1371/journal.pgen.1002375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047444550
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1371/journal.pone.0057266 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016413222
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1615/critrevoncog.v9.i1.40 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068132686
296 rdf:type schema:CreativeWork
297 https://doi.org/10.2144/02324bm01 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075036237
298 rdf:type schema:CreativeWork
299 https://doi.org/10.2741/3961 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070916122
300 rdf:type schema:CreativeWork
301 https://www.grid.ac/institutes/grid.155203.0 schema:alternateName California State Polytechnic University
302 schema:name Department of Biological Science, California State Polytechnic University, 3801 W. Temple Ave, 91768, Pomona, CA, USA
303 rdf:type schema:Organization
304 https://www.grid.ac/institutes/grid.266190.a schema:alternateName University of Colorado Boulder
305 schema:name Department of Molecular and Cellular Biology, University of Arizona, 1007 Lowell St., 85721, Tucson, AZ, USA
306 Department of Molecular, Cellular and Developmental Biology, Currently, University of Colorado Boulder, 347 UCB, CO 80309, Boulder, USA
307 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...