Ancient DNA from lake sediments: Bridging the gap between paleoecology and genetics View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-12

AUTHORS

Lynn L Anderson-Carpenter, Jason S McLachlan, Stephen T Jackson, Melanie Kuch, Candice Y Lumibao, Hendrik N Poinar

ABSTRACT

BACKGROUND: Quaternary plant ecology in much of the world has historically relied on morphological identification of macro- and microfossils from sediments of small freshwater lakes. Here, we report new protocols that reliably yield DNA sequence data from Holocene plant macrofossils and bulk lake sediment used to infer ecological change. This will allow changes in census populations, estimated from fossils and associated sediment, to be directly associated with population genetic changes. RESULTS: We successfully sequenced DNA from 64 samples (out of 126) comprised of bulk sediment and seeds, leaf fragments, budscales, and samaras extracted from Holocene lake sediments in the western Great Lakes region of North America. Overall, DNA yields were low. However, we were able to reliably amplify samples with as few as 10 copies of a short cpDNA fragment with little detectable PCR inhibition. Our success rate was highest for sediments < 2000 years old, but we were able to successfully amplify DNA from samples up to 4600 years old. DNA sequences matched the taxonomic identity of the macrofossil from which they were extracted 79% of the time. Exceptions suggest that DNA molecules from surrounding nearby sediments may permeate or adhere to macrofossils in sediments. CONCLUSIONS: An ability to extract ancient DNA from Holocene sediments potentially allows exciting new insights into the genetic consequences of long-term environmental change. The low DNA copy numbers we found in fossil material and the discovery of multiple sequence variants from single macrofossil extractions highlight the need for careful experimental and laboratory protocols. Further application of these protocols should lead to better understanding of the ecological and evolutionary consequences of environmental change. More... »

PAGES

30

References to SciGraph publications

  • 2008-03. Ancient plant DNA in archaeobotany in VEGETATION HISTORY AND ARCHAEOBOTANY
  • 2010-02. A multidisciplinary study of archaeological grape seeds in THE SCIENCE OF NATURE
  • 2001-05. Ancient DNA in NATURE REVIEWS GENETICS
  • 2000-06. The genetic legacy of the Quaternary ice ages in NATURE
  • 1998-10. A diatom-inferred water depth reconstruction for an Upper Peninsula, Michigan, lake in JOURNAL OF PALEOLIMNOLOGY
  • 1999-01. Pollen dispersal models in Quaternary plant ecology: Assumptions, parameters, and prescriptions in THE BOTANICAL REVIEW
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1471-2148-11-30

    DOI

    http://dx.doi.org/10.1186/1471-2148-11-30

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1029152843

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21272315


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0602", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Ecology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Plant", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fossils", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Geologic Sediments", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Great Lakes Region", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phylogeny", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Notre Dame", 
              "id": "https://www.grid.ac/institutes/grid.131063.6", 
              "name": [
                "Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, IN, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Anderson-Carpenter", 
            "givenName": "Lynn L", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Notre Dame", 
              "id": "https://www.grid.ac/institutes/grid.131063.6", 
              "name": [
                "Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, IN, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "McLachlan", 
            "givenName": "Jason S", 
            "id": "sg:person.01130045624.71", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130045624.71"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Wyoming", 
              "id": "https://www.grid.ac/institutes/grid.135963.b", 
              "name": [
                "Department of Botany and Program in Ecology, University of Wyoming, 82071, Laramie, WY, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jackson", 
            "givenName": "Stephen T", 
            "id": "sg:person.0751455170.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751455170.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "McMaster University", 
              "id": "https://www.grid.ac/institutes/grid.25073.33", 
              "name": [
                "McMaster Ancient DNA Centre, Department of Anthropology and Biology, McMaster University, L8S 4L9, Hamilton, Ontario, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kuch", 
            "givenName": "Melanie", 
            "id": "sg:person.01311102275.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311102275.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Notre Dame", 
              "id": "https://www.grid.ac/institutes/grid.131063.6", 
              "name": [
                "Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, IN, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lumibao", 
            "givenName": "Candice Y", 
            "id": "sg:person.0715542231.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715542231.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "McMaster University", 
              "id": "https://www.grid.ac/institutes/grid.25073.33", 
              "name": [
                "McMaster Ancient DNA Centre, Department of Anthropology and Biology, McMaster University, L8S 4L9, Hamilton, Ontario, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Poinar", 
            "givenName": "Hendrik N", 
            "id": "sg:person.0676355246.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676355246.40"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1046/j.1365-294x.2000.01020.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002156210"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.yqres.2003.07.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002735120"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1890/070160", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004948744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00114-009-0629-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006396386", 
              "https://doi.org/10.1007/s00114-009-0629-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00114-009-0629-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006396386", 
              "https://doi.org/10.1007/s00114-009-0629-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.94.18.9996", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006566652"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.0305-0270.2003.00987.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007373325"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.0305-0270.2003.00987.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007373325"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0606292104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007521475"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-294x.2005.02644.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007650663"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-294x.2005.02644.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007650663"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1469-8137.2006.01740.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007999440"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0378-1127(01)00646-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008149108"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1890/04-1036", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011871545"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pbio.0020290", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014422660"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.genet.37.110801.143214", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014567048"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jas.2005.04.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014943609"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jas.2005.04.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014943609"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00334-007-0125-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016482931", 
              "https://doi.org/10.1007/s00334-007-0125-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00334-007-0125-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016482931", 
              "https://doi.org/10.1007/s00334-007-0125-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1890/0012-9658(2006)87[1124:romcvi]2.0.co;2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017212883"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1469-8137.2005.01360.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019908376"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/29.23.4793", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020812153"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1472-4642.2002.00152.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022080787"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/molbev/msm016", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022853526"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0033-5894(88)90004-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024716542"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1890/02-4045", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025221165"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1502-3885.1980.tb01032.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025435395"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.11.5129", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027768639"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02856557", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028391718", 
              "https://doi.org/10.1007/bf02856557"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02856557", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028391718", 
              "https://doi.org/10.1007/bf02856557"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1523-1739.2007.00676.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029174901"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1523-1739.2007.00676.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029174901"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35016000", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033046918", 
              "https://doi.org/10.1038/35016000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35016000", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033046918", 
              "https://doi.org/10.1038/35016000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0146-6380(02)00168-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034964913"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0146-6380(02)00168-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034964913"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35072071", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037967401", 
              "https://doi.org/10.1038/35072071"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35072071", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037967401", 
              "https://doi.org/10.1038/35072071"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.040453097", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038539222"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1098/rspb.2004.2813", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038798539"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-294x.2009.04092.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040210228"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/molbev/msn095", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040669398"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1469-8137.2006.01781.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042480496"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cub.2008.07.061", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043395348"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jas.2005.06.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043762452"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0912510106", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044467311"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.292.5517.673", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048291442"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/b0-44-452747-8/00225-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048380724"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1007948616511", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050258608", 
              "https://doi.org/10.1023/a:1007948616511"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2307/1942499", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051251882"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1086/284651", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1058594774"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1067814", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062445754"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1084114", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062448079"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1101074", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062450860"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1123360", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062453436"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1172977", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062460079"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1172977", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062460079"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.254.5032.692", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062542923"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.289.5482.1139b", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062570650"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/0959683606069383", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1063897850"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/0959683606069383", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1063897850"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/0959683608093540", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1063898081"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/0959683608093540", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1063898081"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1191/0959683603hl608rp", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064152676"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1191/0959683603hl608rp", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064152676"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1306/74d71807-2b21-11d7-8648000102c1865d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064948896"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2144/000113244", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069096078"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2307/1931618", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069656045"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2307/1938503", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069662411"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2307/1940219", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069663910"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2307/2410719", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069919448"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076065061", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2011-12", 
        "datePublishedReg": "2011-12-01", 
        "description": "BACKGROUND: Quaternary plant ecology in much of the world has historically relied on morphological identification of macro- and microfossils from sediments of small freshwater lakes. Here, we report new protocols that reliably yield DNA sequence data from Holocene plant macrofossils and bulk lake sediment used to infer ecological change. This will allow changes in census populations, estimated from fossils and associated sediment, to be directly associated with population genetic changes.\nRESULTS: We successfully sequenced DNA from 64 samples (out of 126) comprised of bulk sediment and seeds, leaf fragments, budscales, and samaras extracted from Holocene lake sediments in the western Great Lakes region of North America. Overall, DNA yields were low. However, we were able to reliably amplify samples with as few as 10 copies of a short cpDNA fragment with little detectable PCR inhibition. Our success rate was highest for sediments < 2000 years old, but we were able to successfully amplify DNA from samples up to 4600 years old. DNA sequences matched the taxonomic identity of the macrofossil from which they were extracted 79% of the time. Exceptions suggest that DNA molecules from surrounding nearby sediments may permeate or adhere to macrofossils in sediments.\nCONCLUSIONS: An ability to extract ancient DNA from Holocene sediments potentially allows exciting new insights into the genetic consequences of long-term environmental change. The low DNA copy numbers we found in fossil material and the discovery of multiple sequence variants from single macrofossil extractions highlight the need for careful experimental and laboratory protocols. Further application of these protocols should lead to better understanding of the ecological and evolutionary consequences of environmental change.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1186/1471-2148-11-30", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.3044093", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1024249", 
            "issn": [
              "1471-2148"
            ], 
            "name": "BMC Evolutionary Biology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "11"
          }
        ], 
        "name": "Ancient DNA from lake sediments: Bridging the gap between paleoecology and genetics", 
        "pagination": "30", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "8ae12c031b9539e5b426aa10e5166786eb66c3f6ab5b594e60c475452ba43243"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "21272315"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "100966975"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/1471-2148-11-30"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1029152843"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/1471-2148-11-30", 
          "https://app.dimensions.ai/details/publication/pub.1029152843"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T18:19", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000505.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1186%2F1471-2148-11-30"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2148-11-30'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2148-11-30'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2148-11-30'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2148-11-30'


     

    This table displays all metadata directly associated to this object as RDF triples.

    320 TRIPLES      21 PREDICATES      95 URIs      28 LITERALS      16 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/1471-2148-11-30 schema:about N2f46ece8f61a468989b79a198d10882b
    2 N418f004e6bc14dee9defff35985feb35
    3 N43b9b1c07ff045eeb67b23cd7d84858d
    4 N55808b4dc10243c591f8ce44f8c983af
    5 N66d0699e16a147f8966bacc9be0c449d
    6 N6fefad9cbc364789a2d1d29a6d140165
    7 Nad6e28c243d748dc8725774fa89fc2da
    8 anzsrc-for:06
    9 anzsrc-for:0602
    10 schema:author N4c7e825d092441cd9dc1af88d370bc13
    11 schema:citation sg:pub.10.1007/bf02856557
    12 sg:pub.10.1007/s00114-009-0629-3
    13 sg:pub.10.1007/s00334-007-0125-7
    14 sg:pub.10.1023/a:1007948616511
    15 sg:pub.10.1038/35016000
    16 sg:pub.10.1038/35072071
    17 https://app.dimensions.ai/details/publication/pub.1076065061
    18 https://doi.org/10.1016/0033-5894(88)90004-x
    19 https://doi.org/10.1016/b0-44-452747-8/00225-8
    20 https://doi.org/10.1016/j.cub.2008.07.061
    21 https://doi.org/10.1016/j.jas.2005.04.002
    22 https://doi.org/10.1016/j.jas.2005.06.011
    23 https://doi.org/10.1016/j.yqres.2003.07.013
    24 https://doi.org/10.1016/s0146-6380(02)00168-7
    25 https://doi.org/10.1016/s0378-1127(01)00646-6
    26 https://doi.org/10.1046/j.0305-0270.2003.00987.x
    27 https://doi.org/10.1046/j.1365-294x.2000.01020.x
    28 https://doi.org/10.1046/j.1472-4642.2002.00152.x
    29 https://doi.org/10.1073/pnas.040453097
    30 https://doi.org/10.1073/pnas.0606292104
    31 https://doi.org/10.1073/pnas.0912510106
    32 https://doi.org/10.1073/pnas.91.11.5129
    33 https://doi.org/10.1073/pnas.94.18.9996
    34 https://doi.org/10.1086/284651
    35 https://doi.org/10.1093/molbev/msm016
    36 https://doi.org/10.1093/molbev/msn095
    37 https://doi.org/10.1093/nar/29.23.4793
    38 https://doi.org/10.1098/rspb.2004.2813
    39 https://doi.org/10.1111/j.1365-294x.2005.02644.x
    40 https://doi.org/10.1111/j.1365-294x.2009.04092.x
    41 https://doi.org/10.1111/j.1469-8137.2005.01360.x
    42 https://doi.org/10.1111/j.1469-8137.2006.01740.x
    43 https://doi.org/10.1111/j.1469-8137.2006.01781.x
    44 https://doi.org/10.1111/j.1502-3885.1980.tb01032.x
    45 https://doi.org/10.1111/j.1523-1739.2007.00676.x
    46 https://doi.org/10.1126/science.1067814
    47 https://doi.org/10.1126/science.1084114
    48 https://doi.org/10.1126/science.1101074
    49 https://doi.org/10.1126/science.1123360
    50 https://doi.org/10.1126/science.1172977
    51 https://doi.org/10.1126/science.254.5032.692
    52 https://doi.org/10.1126/science.289.5482.1139b
    53 https://doi.org/10.1126/science.292.5517.673
    54 https://doi.org/10.1146/annurev.genet.37.110801.143214
    55 https://doi.org/10.1177/0959683606069383
    56 https://doi.org/10.1177/0959683608093540
    57 https://doi.org/10.1191/0959683603hl608rp
    58 https://doi.org/10.1306/74d71807-2b21-11d7-8648000102c1865d
    59 https://doi.org/10.1371/journal.pbio.0020290
    60 https://doi.org/10.1890/0012-9658(2006)87[1124:romcvi]2.0.co;2
    61 https://doi.org/10.1890/02-4045
    62 https://doi.org/10.1890/04-1036
    63 https://doi.org/10.1890/070160
    64 https://doi.org/10.2144/000113244
    65 https://doi.org/10.2307/1931618
    66 https://doi.org/10.2307/1938503
    67 https://doi.org/10.2307/1940219
    68 https://doi.org/10.2307/1942499
    69 https://doi.org/10.2307/2410719
    70 schema:datePublished 2011-12
    71 schema:datePublishedReg 2011-12-01
    72 schema:description BACKGROUND: Quaternary plant ecology in much of the world has historically relied on morphological identification of macro- and microfossils from sediments of small freshwater lakes. Here, we report new protocols that reliably yield DNA sequence data from Holocene plant macrofossils and bulk lake sediment used to infer ecological change. This will allow changes in census populations, estimated from fossils and associated sediment, to be directly associated with population genetic changes. RESULTS: We successfully sequenced DNA from 64 samples (out of 126) comprised of bulk sediment and seeds, leaf fragments, budscales, and samaras extracted from Holocene lake sediments in the western Great Lakes region of North America. Overall, DNA yields were low. However, we were able to reliably amplify samples with as few as 10 copies of a short cpDNA fragment with little detectable PCR inhibition. Our success rate was highest for sediments < 2000 years old, but we were able to successfully amplify DNA from samples up to 4600 years old. DNA sequences matched the taxonomic identity of the macrofossil from which they were extracted 79% of the time. Exceptions suggest that DNA molecules from surrounding nearby sediments may permeate or adhere to macrofossils in sediments. CONCLUSIONS: An ability to extract ancient DNA from Holocene sediments potentially allows exciting new insights into the genetic consequences of long-term environmental change. The low DNA copy numbers we found in fossil material and the discovery of multiple sequence variants from single macrofossil extractions highlight the need for careful experimental and laboratory protocols. Further application of these protocols should lead to better understanding of the ecological and evolutionary consequences of environmental change.
    73 schema:genre research_article
    74 schema:inLanguage en
    75 schema:isAccessibleForFree true
    76 schema:isPartOf N172a1568184744cead4028796b15d80e
    77 Nac4c2f5d349d452c8bfce9cc69057f5d
    78 sg:journal.1024249
    79 schema:name Ancient DNA from lake sediments: Bridging the gap between paleoecology and genetics
    80 schema:pagination 30
    81 schema:productId N3016e91ed11b49eb9d4d88d11b40f094
    82 N6c2316392b2041dfb13bcae712d97cdc
    83 Na378e46f1b2c447f977e27dbda74f63a
    84 Nbc64faf37f6c4fb2b181a20cabf870c4
    85 Nc3631c0839764988a01a00c3bcc6d9f2
    86 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029152843
    87 https://doi.org/10.1186/1471-2148-11-30
    88 schema:sdDatePublished 2019-04-10T18:19
    89 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    90 schema:sdPublisher Nad7aafb9ad234eb3bd68f63c17ce8dc9
    91 schema:url http://link.springer.com/10.1186%2F1471-2148-11-30
    92 sgo:license sg:explorer/license/
    93 sgo:sdDataset articles
    94 rdf:type schema:ScholarlyArticle
    95 N13a12d7815684399a94531f9bd3c6bbd rdf:first sg:person.01311102275.09
    96 rdf:rest N63b6b50b6bfd488ea676eb1b20033934
    97 N172a1568184744cead4028796b15d80e schema:issueNumber 1
    98 rdf:type schema:PublicationIssue
    99 N248fc7db2f64428680f7ac4458f15b60 schema:affiliation https://www.grid.ac/institutes/grid.131063.6
    100 schema:familyName Anderson-Carpenter
    101 schema:givenName Lynn L
    102 rdf:type schema:Person
    103 N2f46ece8f61a468989b79a198d10882b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Geologic Sediments
    105 rdf:type schema:DefinedTerm
    106 N3016e91ed11b49eb9d4d88d11b40f094 schema:name pubmed_id
    107 schema:value 21272315
    108 rdf:type schema:PropertyValue
    109 N418f004e6bc14dee9defff35985feb35 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Fossils
    111 rdf:type schema:DefinedTerm
    112 N43b9b1c07ff045eeb67b23cd7d84858d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Phylogeny
    114 rdf:type schema:DefinedTerm
    115 N4c7e825d092441cd9dc1af88d370bc13 rdf:first N248fc7db2f64428680f7ac4458f15b60
    116 rdf:rest N6faaa1b3d32e4e809c839dc38d771e62
    117 N55808b4dc10243c591f8ce44f8c983af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name DNA, Plant
    119 rdf:type schema:DefinedTerm
    120 N63b6b50b6bfd488ea676eb1b20033934 rdf:first sg:person.0715542231.05
    121 rdf:rest Nc782f144e72c43b9a8e56160665a86a6
    122 N66d0699e16a147f8966bacc9be0c449d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Plants
    124 rdf:type schema:DefinedTerm
    125 N6c2316392b2041dfb13bcae712d97cdc schema:name readcube_id
    126 schema:value 8ae12c031b9539e5b426aa10e5166786eb66c3f6ab5b594e60c475452ba43243
    127 rdf:type schema:PropertyValue
    128 N6faaa1b3d32e4e809c839dc38d771e62 rdf:first sg:person.01130045624.71
    129 rdf:rest Nd54d97ccec0145949ffc6499f5cb6c12
    130 N6fefad9cbc364789a2d1d29a6d140165 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    131 schema:name Great Lakes Region
    132 rdf:type schema:DefinedTerm
    133 Na378e46f1b2c447f977e27dbda74f63a schema:name nlm_unique_id
    134 schema:value 100966975
    135 rdf:type schema:PropertyValue
    136 Nac4c2f5d349d452c8bfce9cc69057f5d schema:volumeNumber 11
    137 rdf:type schema:PublicationVolume
    138 Nad6e28c243d748dc8725774fa89fc2da schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    139 schema:name Molecular Sequence Data
    140 rdf:type schema:DefinedTerm
    141 Nad7aafb9ad234eb3bd68f63c17ce8dc9 schema:name Springer Nature - SN SciGraph project
    142 rdf:type schema:Organization
    143 Nbc64faf37f6c4fb2b181a20cabf870c4 schema:name dimensions_id
    144 schema:value pub.1029152843
    145 rdf:type schema:PropertyValue
    146 Nc3631c0839764988a01a00c3bcc6d9f2 schema:name doi
    147 schema:value 10.1186/1471-2148-11-30
    148 rdf:type schema:PropertyValue
    149 Nc782f144e72c43b9a8e56160665a86a6 rdf:first sg:person.0676355246.40
    150 rdf:rest rdf:nil
    151 Nd54d97ccec0145949ffc6499f5cb6c12 rdf:first sg:person.0751455170.02
    152 rdf:rest N13a12d7815684399a94531f9bd3c6bbd
    153 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    154 schema:name Biological Sciences
    155 rdf:type schema:DefinedTerm
    156 anzsrc-for:0602 schema:inDefinedTermSet anzsrc-for:
    157 schema:name Ecology
    158 rdf:type schema:DefinedTerm
    159 sg:grant.3044093 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2148-11-30
    160 rdf:type schema:MonetaryGrant
    161 sg:journal.1024249 schema:issn 1471-2148
    162 schema:name BMC Evolutionary Biology
    163 rdf:type schema:Periodical
    164 sg:person.01130045624.71 schema:affiliation https://www.grid.ac/institutes/grid.131063.6
    165 schema:familyName McLachlan
    166 schema:givenName Jason S
    167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130045624.71
    168 rdf:type schema:Person
    169 sg:person.01311102275.09 schema:affiliation https://www.grid.ac/institutes/grid.25073.33
    170 schema:familyName Kuch
    171 schema:givenName Melanie
    172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311102275.09
    173 rdf:type schema:Person
    174 sg:person.0676355246.40 schema:affiliation https://www.grid.ac/institutes/grid.25073.33
    175 schema:familyName Poinar
    176 schema:givenName Hendrik N
    177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0676355246.40
    178 rdf:type schema:Person
    179 sg:person.0715542231.05 schema:affiliation https://www.grid.ac/institutes/grid.131063.6
    180 schema:familyName Lumibao
    181 schema:givenName Candice Y
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715542231.05
    183 rdf:type schema:Person
    184 sg:person.0751455170.02 schema:affiliation https://www.grid.ac/institutes/grid.135963.b
    185 schema:familyName Jackson
    186 schema:givenName Stephen T
    187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751455170.02
    188 rdf:type schema:Person
    189 sg:pub.10.1007/bf02856557 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028391718
    190 https://doi.org/10.1007/bf02856557
    191 rdf:type schema:CreativeWork
    192 sg:pub.10.1007/s00114-009-0629-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006396386
    193 https://doi.org/10.1007/s00114-009-0629-3
    194 rdf:type schema:CreativeWork
    195 sg:pub.10.1007/s00334-007-0125-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016482931
    196 https://doi.org/10.1007/s00334-007-0125-7
    197 rdf:type schema:CreativeWork
    198 sg:pub.10.1023/a:1007948616511 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050258608
    199 https://doi.org/10.1023/a:1007948616511
    200 rdf:type schema:CreativeWork
    201 sg:pub.10.1038/35016000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033046918
    202 https://doi.org/10.1038/35016000
    203 rdf:type schema:CreativeWork
    204 sg:pub.10.1038/35072071 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037967401
    205 https://doi.org/10.1038/35072071
    206 rdf:type schema:CreativeWork
    207 https://app.dimensions.ai/details/publication/pub.1076065061 schema:CreativeWork
    208 https://doi.org/10.1016/0033-5894(88)90004-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024716542
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1016/b0-44-452747-8/00225-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048380724
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1016/j.cub.2008.07.061 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043395348
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1016/j.jas.2005.04.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014943609
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1016/j.jas.2005.06.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043762452
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1016/j.yqres.2003.07.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002735120
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1016/s0146-6380(02)00168-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034964913
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1016/s0378-1127(01)00646-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008149108
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1046/j.0305-0270.2003.00987.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007373325
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1046/j.1365-294x.2000.01020.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002156210
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1046/j.1472-4642.2002.00152.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022080787
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1073/pnas.040453097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038539222
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1073/pnas.0606292104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007521475
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1073/pnas.0912510106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044467311
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1073/pnas.91.11.5129 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027768639
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1073/pnas.94.18.9996 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006566652
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1086/284651 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058594774
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1093/molbev/msm016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022853526
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1093/molbev/msn095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040669398
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1093/nar/29.23.4793 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020812153
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1098/rspb.2004.2813 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038798539
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1111/j.1365-294x.2005.02644.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007650663
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1111/j.1365-294x.2009.04092.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040210228
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1111/j.1469-8137.2005.01360.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019908376
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1111/j.1469-8137.2006.01740.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007999440
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1111/j.1469-8137.2006.01781.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042480496
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1111/j.1502-3885.1980.tb01032.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1025435395
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1111/j.1523-1739.2007.00676.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1029174901
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1126/science.1067814 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062445754
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1126/science.1084114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062448079
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1126/science.1101074 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062450860
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1126/science.1123360 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062453436
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1126/science.1172977 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062460079
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1126/science.254.5032.692 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062542923
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1126/science.289.5482.1139b schema:sameAs https://app.dimensions.ai/details/publication/pub.1062570650
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1126/science.292.5517.673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048291442
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1146/annurev.genet.37.110801.143214 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014567048
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1177/0959683606069383 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063897850
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1177/0959683608093540 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063898081
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1191/0959683603hl608rp schema:sameAs https://app.dimensions.ai/details/publication/pub.1064152676
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1306/74d71807-2b21-11d7-8648000102c1865d schema:sameAs https://app.dimensions.ai/details/publication/pub.1064948896
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1371/journal.pbio.0020290 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014422660
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1890/0012-9658(2006)87[1124:romcvi]2.0.co;2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017212883
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1890/02-4045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025221165
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1890/04-1036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011871545
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1890/070160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004948744
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.2144/000113244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069096078
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.2307/1931618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069656045
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.2307/1938503 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069662411
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.2307/1940219 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069663910
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.2307/1942499 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051251882
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.2307/2410719 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069919448
    311 rdf:type schema:CreativeWork
    312 https://www.grid.ac/institutes/grid.131063.6 schema:alternateName University of Notre Dame
    313 schema:name Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, IN, USA
    314 rdf:type schema:Organization
    315 https://www.grid.ac/institutes/grid.135963.b schema:alternateName University of Wyoming
    316 schema:name Department of Botany and Program in Ecology, University of Wyoming, 82071, Laramie, WY, USA
    317 rdf:type schema:Organization
    318 https://www.grid.ac/institutes/grid.25073.33 schema:alternateName McMaster University
    319 schema:name McMaster Ancient DNA Centre, Department of Anthropology and Biology, McMaster University, L8S 4L9, Hamilton, Ontario, Canada
    320 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...