Photo-activation of the hydrophobic probe iodonaphthylazide in cells alters membrane protein function leading to cell death View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-12

AUTHORS

Mathias Viard, Himanshu Garg, Robert Blumenthal, Yossef Raviv

ABSTRACT

BACKGROUND: Photo-activation of the hydrophobic membrane probe 1, 5 iodonaphthylazide (INA) by irradiation with UV light (310-380 nm) results in the covalent modification of transmembrane anchors of membrane proteins. This unique selectivity of INA towards the transmembrane anchor has been exploited to specifically label proteins inserted in membranes. Previously, we have demonstrated that photo-activation of INA in enveloped viruses resulted in the inhibition of viral membrane protein-induced membrane fusion and viral entry into cells. In this study we show that photo-activation of INA in various cell lines, including those over-expressing the multi-drug resistance transporters MRP1 or Pgp, leads to cell death. We analyzed mechanisms of cell killing by INA-UV treatment. The effects of INA-UV treatment on signaling via various cell surface receptors, on the activity of the multi-drug resistance transporter MRP1 and on membrane protein lateral mobility were also investigated. RESULTS: INA treatment of various cell lines followed by irradiation with UV light (310-380 nm) resulted in loss of cell viability in a dose dependent manner. The mechanism of cell death appeared to be apoptosis as indicated by phosphatidylserine exposure, mitochondrial depolarization and DNA fragmentation. Inhibition by pan-caspase inhibitors and cleavage of caspase specific substrates indicated that at low concentrations of INA apoptosis was caspase dependent. The INA-UV treatment showed similar cell killing efficacy in cells over-expressing MRP1 function as control cells. Efflux of an MRP1 substrate was blocked by INA-UV treatment of the MRP1-overexpressing cells. Although INA-UV treatment resulted in inhibition of calcium mobilization triggered by chemokine receptor signaling, Akt phosphorylation triggered by IGF1 receptor signaling was enhanced. Furthermore, fluorescence recovery after photobleaching experiments indicated that INA-UV treatment resulted in reduced lateral mobility of a seven transmembrane G protein-coupled receptor. CONCLUSION: INA is a photo-activable agent that induces apoptosis in various cancer cell lines. It reacts with membrane proteins to alter the normal physiological function resulting in apoptosis. This activity of INA maybe exploited for use as an anti-cancer agent. More... »

PAGES

21

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1471-2121-10-21

DOI

http://dx.doi.org/10.1186/1471-2121-10-21

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049968743

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19323821


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Azides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caspases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Dyes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Multidrug Resistance-Associated Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, G-Protein-Coupled", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ultraviolet Rays", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Cancer Institute", 
          "id": "https://www.grid.ac/institutes/grid.48336.3a", 
          "name": [
            "Nanobiology Program, Center of Cancer Research, National Cancer Institute, Frederick, Maryland, USA", 
            "Basic Research Program, SAIC-Frederick Inc, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Viard", 
        "givenName": "Mathias", 
        "id": "sg:person.01047331332.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047331332.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Cancer Research", 
          "id": "https://www.grid.ac/institutes/grid.417768.b", 
          "name": [
            "Nanobiology Program, Center of Cancer Research, National Cancer Institute, Frederick, Maryland, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Garg", 
        "givenName": "Himanshu", 
        "id": "sg:person.0677751273.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677751273.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Cancer Research", 
          "id": "https://www.grid.ac/institutes/grid.417768.b", 
          "name": [
            "Nanobiology Program, Center of Cancer Research, National Cancer Institute, Frederick, Maryland, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Blumenthal", 
        "givenName": "Robert", 
        "id": "sg:person.0622515432.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622515432.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Cancer Institute", 
          "id": "https://www.grid.ac/institutes/grid.48336.3a", 
          "name": [
            "Nanobiology Program, Center of Cancer Research, National Cancer Institute, Frederick, Maryland, USA", 
            "Basic Research Program, SAIC-Frederick Inc, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Raviv", 
        "givenName": "Yossef", 
        "id": "sg:person.01251671127.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251671127.06"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/ijc.22024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000262085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/viro.2001.1237", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002052911"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/09687680701613713", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003218678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bi.62.070193.002125", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003490677"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35036052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004200173", 
          "https://doi.org/10.1038/35036052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35036052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004200173", 
          "https://doi.org/10.1038/35036052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35036052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004200173", 
          "https://doi.org/10.1038/35036052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni0403-308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006069267", 
          "https://doi.org/10.1038/ni0403-308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni0403-308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006069267", 
          "https://doi.org/10.1038/ni0403-308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/ec.00114-06", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009156224"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.65.3.675", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009746539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200309101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009857792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.77.12.7067-7077.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011461355"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/09687689509038509", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011656281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/18.22.6211", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012199783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3150021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012695534"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3150021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012695534"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0005-2736(96)00237-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012980274"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3260001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014115395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3260001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014115395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.79.19.12394-12400.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014547247"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2404357", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015388322", 
          "https://doi.org/10.1038/sj.leu.2404357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2404357", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015388322", 
          "https://doi.org/10.1038/sj.leu.2404357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201947", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018076908", 
          "https://doi.org/10.1038/sj.onc.1201947"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201947", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018076908", 
          "https://doi.org/10.1038/sj.onc.1201947"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10495-006-0190-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018170569", 
          "https://doi.org/10.1007/s10495-006-0190-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10495-006-0190-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018170569", 
          "https://doi.org/10.1007/s10495-006-0190-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/289806a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019302886", 
          "https://doi.org/10.1038/289806a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4401400", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019493693", 
          "https://doi.org/10.1038/sj.cdd.4401400"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4401400", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019493693", 
          "https://doi.org/10.1038/sj.cdd.4401400"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1517/17425255.3.6.805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022761062"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35073068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024425726", 
          "https://doi.org/10.1038/35073068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35073068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024425726", 
          "https://doi.org/10.1038/35073068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(02)00973-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024839636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1349-7006.2007.00606.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027380026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(94)90344-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028334480"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.32.19041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030172522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.semcancer.2003.10.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032956206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.02233-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034612310"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(95)00677-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035310560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.febslet.2005.11.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036939610"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0005-2736(98)00014-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039049398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01534700", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039221063", 
          "https://doi.org/10.1007/bf01534700"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.20030267", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041106320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.17.6103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042356499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1743-422x-3-104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043875359", 
          "https://doi.org/10.1186/1743-422x-3-104"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3350597", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044124517"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3350597", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044124517"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2007.04.115", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048069924"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/371346a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048646533", 
          "https://doi.org/10.1038/371346a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2005-08-3182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050475732"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.febslet.2005.12.060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052553989"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/520605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053714396"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00298a016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055169195"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00558a001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055180713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00601a020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055182261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi701920f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055207971"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi701920f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055207971"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9702851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055213621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9702851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055213621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1360704", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062477732"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.663661", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062639318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/ijo.27.5.1329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071515872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077437704", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077945666", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079981956", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpendo.1978.235.2.e97", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1081998480"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082689984", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1996.tb01045.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083023835"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-12", 
    "datePublishedReg": "2009-12-01", 
    "description": "BACKGROUND: Photo-activation of the hydrophobic membrane probe 1, 5 iodonaphthylazide (INA) by irradiation with UV light (310-380 nm) results in the covalent modification of transmembrane anchors of membrane proteins. This unique selectivity of INA towards the transmembrane anchor has been exploited to specifically label proteins inserted in membranes. Previously, we have demonstrated that photo-activation of INA in enveloped viruses resulted in the inhibition of viral membrane protein-induced membrane fusion and viral entry into cells. In this study we show that photo-activation of INA in various cell lines, including those over-expressing the multi-drug resistance transporters MRP1 or Pgp, leads to cell death. We analyzed mechanisms of cell killing by INA-UV treatment. The effects of INA-UV treatment on signaling via various cell surface receptors, on the activity of the multi-drug resistance transporter MRP1 and on membrane protein lateral mobility were also investigated.\nRESULTS: INA treatment of various cell lines followed by irradiation with UV light (310-380 nm) resulted in loss of cell viability in a dose dependent manner. The mechanism of cell death appeared to be apoptosis as indicated by phosphatidylserine exposure, mitochondrial depolarization and DNA fragmentation. Inhibition by pan-caspase inhibitors and cleavage of caspase specific substrates indicated that at low concentrations of INA apoptosis was caspase dependent. The INA-UV treatment showed similar cell killing efficacy in cells over-expressing MRP1 function as control cells. Efflux of an MRP1 substrate was blocked by INA-UV treatment of the MRP1-overexpressing cells. Although INA-UV treatment resulted in inhibition of calcium mobilization triggered by chemokine receptor signaling, Akt phosphorylation triggered by IGF1 receptor signaling was enhanced. Furthermore, fluorescence recovery after photobleaching experiments indicated that INA-UV treatment resulted in reduced lateral mobility of a seven transmembrane G protein-coupled receptor.\nCONCLUSION: INA is a photo-activable agent that induces apoptosis in various cancer cell lines. It reacts with membrane proteins to alter the normal physiological function resulting in apoptosis. This activity of INA maybe exploited for use as an anti-cancer agent.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1471-2121-10-21", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2723768", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5246872", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2723574", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1024246", 
        "issn": [
          "2330-0175", 
          "1471-2121"
        ], 
        "name": "BMC Cell Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "name": "Photo-activation of the hydrophobic probe iodonaphthylazide in cells alters membrane protein function leading to cell death", 
    "pagination": "21", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ee08fb23c3baa4123ac25dd3ce12ece06e0d600f4bd5311f0cc1ce7fe5556633"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19323821"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100966972"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1471-2121-10-21"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1049968743"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1471-2121-10-21", 
      "https://app.dimensions.ai/details/publication/pub.1049968743"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:23", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000508.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1471-2121-10-21"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1471-2121-10-21'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1471-2121-10-21'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1471-2121-10-21'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1471-2121-10-21'


 

This table displays all metadata directly associated to this object as RDF triples.

319 TRIPLES      21 PREDICATES      96 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1471-2121-10-21 schema:about N03b9e1604fe64c71bcc7b9124cd21b91
2 N0ce75afd74cc4628b5e9b8dd51e58bc3
3 N0d3ebb96f5e04898acaa302fbe974d6a
4 N1b6f101f00604feb99622abc0044f5c2
5 N5328064d8c354e059bd4ab77748219ce
6 N94b9f7e40a46463a85010ac50418cdf2
7 Na08ebc77628247e19fb155c1d807dc49
8 Nb67f580b2ac94834819692b7081a070b
9 Nbb67527d20224b529a767eb68da23d36
10 Nc79e97bb53f249b4933c52723b7336f9
11 Nd899bec4869047a3b150b2fd711d5e25
12 anzsrc-for:06
13 anzsrc-for:0601
14 schema:author Ne3288c8c80c54e1ea8ce1ee74674a746
15 schema:citation sg:pub.10.1007/bf01534700
16 sg:pub.10.1007/s10495-006-0190-x
17 sg:pub.10.1038/289806a0
18 sg:pub.10.1038/35036052
19 sg:pub.10.1038/35073068
20 sg:pub.10.1038/371346a0
21 sg:pub.10.1038/ni0403-308
22 sg:pub.10.1038/sj.cdd.4401400
23 sg:pub.10.1038/sj.leu.2404357
24 sg:pub.10.1038/sj.onc.1201947
25 sg:pub.10.1186/1743-422x-3-104
26 https://app.dimensions.ai/details/publication/pub.1077437704
27 https://app.dimensions.ai/details/publication/pub.1077945666
28 https://app.dimensions.ai/details/publication/pub.1079981956
29 https://app.dimensions.ai/details/publication/pub.1082689984
30 https://doi.org/10.1002/ijc.22024
31 https://doi.org/10.1002/j.1460-2075.1996.tb01045.x
32 https://doi.org/10.1006/viro.2001.1237
33 https://doi.org/10.1016/0014-5793(95)00677-2
34 https://doi.org/10.1016/0092-8674(94)90344-1
35 https://doi.org/10.1016/j.bbrc.2007.04.115
36 https://doi.org/10.1016/j.febslet.2005.11.040
37 https://doi.org/10.1016/j.febslet.2005.12.060
38 https://doi.org/10.1016/j.semcancer.2003.10.003
39 https://doi.org/10.1016/s0005-2736(96)00237-4
40 https://doi.org/10.1016/s0005-2736(98)00014-5
41 https://doi.org/10.1016/s0960-9822(02)00973-9
42 https://doi.org/10.1021/bi00298a016
43 https://doi.org/10.1021/bi00558a001
44 https://doi.org/10.1021/bi00601a020
45 https://doi.org/10.1021/bi701920f
46 https://doi.org/10.1021/bi9702851
47 https://doi.org/10.1042/bj3150021
48 https://doi.org/10.1042/bj3260001
49 https://doi.org/10.1042/bj3350597
50 https://doi.org/10.1073/pnas.84.17.6103
51 https://doi.org/10.1074/jbc.270.32.19041
52 https://doi.org/10.1080/09687680701613713
53 https://doi.org/10.1083/jcb.200309101
54 https://doi.org/10.1084/jem.20030267
55 https://doi.org/10.1086/520605
56 https://doi.org/10.1093/emboj/18.22.6211
57 https://doi.org/10.1111/j.1349-7006.2007.00606.x
58 https://doi.org/10.1124/mol.65.3.675
59 https://doi.org/10.1126/science.1360704
60 https://doi.org/10.1126/science.663661
61 https://doi.org/10.1128/ec.00114-06
62 https://doi.org/10.1128/jvi.02233-07
63 https://doi.org/10.1128/jvi.77.12.7067-7077.2003
64 https://doi.org/10.1128/jvi.79.19.12394-12400.2005
65 https://doi.org/10.1146/annurev.bi.62.070193.002125
66 https://doi.org/10.1152/ajpendo.1978.235.2.e97
67 https://doi.org/10.1182/blood-2005-08-3182
68 https://doi.org/10.1517/17425255.3.6.805
69 https://doi.org/10.3109/09687689509038509
70 https://doi.org/10.3892/ijo.27.5.1329
71 schema:datePublished 2009-12
72 schema:datePublishedReg 2009-12-01
73 schema:description BACKGROUND: Photo-activation of the hydrophobic membrane probe 1, 5 iodonaphthylazide (INA) by irradiation with UV light (310-380 nm) results in the covalent modification of transmembrane anchors of membrane proteins. This unique selectivity of INA towards the transmembrane anchor has been exploited to specifically label proteins inserted in membranes. Previously, we have demonstrated that photo-activation of INA in enveloped viruses resulted in the inhibition of viral membrane protein-induced membrane fusion and viral entry into cells. In this study we show that photo-activation of INA in various cell lines, including those over-expressing the multi-drug resistance transporters MRP1 or Pgp, leads to cell death. We analyzed mechanisms of cell killing by INA-UV treatment. The effects of INA-UV treatment on signaling via various cell surface receptors, on the activity of the multi-drug resistance transporter MRP1 and on membrane protein lateral mobility were also investigated. RESULTS: INA treatment of various cell lines followed by irradiation with UV light (310-380 nm) resulted in loss of cell viability in a dose dependent manner. The mechanism of cell death appeared to be apoptosis as indicated by phosphatidylserine exposure, mitochondrial depolarization and DNA fragmentation. Inhibition by pan-caspase inhibitors and cleavage of caspase specific substrates indicated that at low concentrations of INA apoptosis was caspase dependent. The INA-UV treatment showed similar cell killing efficacy in cells over-expressing MRP1 function as control cells. Efflux of an MRP1 substrate was blocked by INA-UV treatment of the MRP1-overexpressing cells. Although INA-UV treatment resulted in inhibition of calcium mobilization triggered by chemokine receptor signaling, Akt phosphorylation triggered by IGF1 receptor signaling was enhanced. Furthermore, fluorescence recovery after photobleaching experiments indicated that INA-UV treatment resulted in reduced lateral mobility of a seven transmembrane G protein-coupled receptor. CONCLUSION: INA is a photo-activable agent that induces apoptosis in various cancer cell lines. It reacts with membrane proteins to alter the normal physiological function resulting in apoptosis. This activity of INA maybe exploited for use as an anti-cancer agent.
74 schema:genre research_article
75 schema:inLanguage en
76 schema:isAccessibleForFree true
77 schema:isPartOf N01da12a3fd2b47cfad52b7a04aaefa60
78 N373780488b0f434c97b7563e4520f323
79 sg:journal.1024246
80 schema:name Photo-activation of the hydrophobic probe iodonaphthylazide in cells alters membrane protein function leading to cell death
81 schema:pagination 21
82 schema:productId N03c7f9a8f9744ff1bae73ad7c1c9f7c9
83 N212a2d3efd044ff48e6c14e729dc1fc4
84 N4041750a530f4685ba7a7dbc1cdf809a
85 N4dbe665c10654d3083252456f3dd5373
86 N9c2c25f69f304c31b6e996d973928a93
87 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049968743
88 https://doi.org/10.1186/1471-2121-10-21
89 schema:sdDatePublished 2019-04-10T23:23
90 schema:sdLicense https://scigraph.springernature.com/explorer/license/
91 schema:sdPublisher N75f6f28de4b9492da281b427461487f8
92 schema:url http://link.springer.com/10.1186%2F1471-2121-10-21
93 sgo:license sg:explorer/license/
94 sgo:sdDataset articles
95 rdf:type schema:ScholarlyArticle
96 N01da12a3fd2b47cfad52b7a04aaefa60 schema:issueNumber 1
97 rdf:type schema:PublicationIssue
98 N03b9e1604fe64c71bcc7b9124cd21b91 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Membrane Proteins
100 rdf:type schema:DefinedTerm
101 N03c7f9a8f9744ff1bae73ad7c1c9f7c9 schema:name pubmed_id
102 schema:value 19323821
103 rdf:type schema:PropertyValue
104 N0ce75afd74cc4628b5e9b8dd51e58bc3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Caspases
106 rdf:type schema:DefinedTerm
107 N0d3ebb96f5e04898acaa302fbe974d6a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Cell Line, Tumor
109 rdf:type schema:DefinedTerm
110 N10384107d017482ea94ca3eb3ed1d07f rdf:first sg:person.0622515432.21
111 rdf:rest N50e4efbaae2e46768e0a2b7c01666ab6
112 N1b6f101f00604feb99622abc0044f5c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Humans
114 rdf:type schema:DefinedTerm
115 N212a2d3efd044ff48e6c14e729dc1fc4 schema:name readcube_id
116 schema:value ee08fb23c3baa4123ac25dd3ce12ece06e0d600f4bd5311f0cc1ce7fe5556633
117 rdf:type schema:PropertyValue
118 N373780488b0f434c97b7563e4520f323 schema:volumeNumber 10
119 rdf:type schema:PublicationVolume
120 N4041750a530f4685ba7a7dbc1cdf809a schema:name doi
121 schema:value 10.1186/1471-2121-10-21
122 rdf:type schema:PropertyValue
123 N4dbe665c10654d3083252456f3dd5373 schema:name nlm_unique_id
124 schema:value 100966972
125 rdf:type schema:PropertyValue
126 N50e4efbaae2e46768e0a2b7c01666ab6 rdf:first sg:person.01251671127.06
127 rdf:rest rdf:nil
128 N5328064d8c354e059bd4ab77748219ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Ultraviolet Rays
130 rdf:type schema:DefinedTerm
131 N75f6f28de4b9492da281b427461487f8 schema:name Springer Nature - SN SciGraph project
132 rdf:type schema:Organization
133 N94b9f7e40a46463a85010ac50418cdf2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Azides
135 rdf:type schema:DefinedTerm
136 N9c2c25f69f304c31b6e996d973928a93 schema:name dimensions_id
137 schema:value pub.1049968743
138 rdf:type schema:PropertyValue
139 Na08ebc77628247e19fb155c1d807dc49 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Receptors, G-Protein-Coupled
141 rdf:type schema:DefinedTerm
142 Na2b8937b344246b2bfe64941f3d1573e rdf:first sg:person.0677751273.15
143 rdf:rest N10384107d017482ea94ca3eb3ed1d07f
144 Nb67f580b2ac94834819692b7081a070b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Signal Transduction
146 rdf:type schema:DefinedTerm
147 Nbb67527d20224b529a767eb68da23d36 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Apoptosis
149 rdf:type schema:DefinedTerm
150 Nc79e97bb53f249b4933c52723b7336f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Multidrug Resistance-Associated Proteins
152 rdf:type schema:DefinedTerm
153 Nd899bec4869047a3b150b2fd711d5e25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Fluorescent Dyes
155 rdf:type schema:DefinedTerm
156 Ne3288c8c80c54e1ea8ce1ee74674a746 rdf:first sg:person.01047331332.06
157 rdf:rest Na2b8937b344246b2bfe64941f3d1573e
158 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
159 schema:name Biological Sciences
160 rdf:type schema:DefinedTerm
161 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
162 schema:name Biochemistry and Cell Biology
163 rdf:type schema:DefinedTerm
164 sg:grant.2723574 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2121-10-21
165 rdf:type schema:MonetaryGrant
166 sg:grant.2723768 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2121-10-21
167 rdf:type schema:MonetaryGrant
168 sg:grant.5246872 http://pending.schema.org/fundedItem sg:pub.10.1186/1471-2121-10-21
169 rdf:type schema:MonetaryGrant
170 sg:journal.1024246 schema:issn 1471-2121
171 2330-0175
172 schema:name BMC Cell Biology
173 rdf:type schema:Periodical
174 sg:person.01047331332.06 schema:affiliation https://www.grid.ac/institutes/grid.48336.3a
175 schema:familyName Viard
176 schema:givenName Mathias
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047331332.06
178 rdf:type schema:Person
179 sg:person.01251671127.06 schema:affiliation https://www.grid.ac/institutes/grid.48336.3a
180 schema:familyName Raviv
181 schema:givenName Yossef
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251671127.06
183 rdf:type schema:Person
184 sg:person.0622515432.21 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
185 schema:familyName Blumenthal
186 schema:givenName Robert
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622515432.21
188 rdf:type schema:Person
189 sg:person.0677751273.15 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
190 schema:familyName Garg
191 schema:givenName Himanshu
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677751273.15
193 rdf:type schema:Person
194 sg:pub.10.1007/bf01534700 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039221063
195 https://doi.org/10.1007/bf01534700
196 rdf:type schema:CreativeWork
197 sg:pub.10.1007/s10495-006-0190-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1018170569
198 https://doi.org/10.1007/s10495-006-0190-x
199 rdf:type schema:CreativeWork
200 sg:pub.10.1038/289806a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019302886
201 https://doi.org/10.1038/289806a0
202 rdf:type schema:CreativeWork
203 sg:pub.10.1038/35036052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004200173
204 https://doi.org/10.1038/35036052
205 rdf:type schema:CreativeWork
206 sg:pub.10.1038/35073068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024425726
207 https://doi.org/10.1038/35073068
208 rdf:type schema:CreativeWork
209 sg:pub.10.1038/371346a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048646533
210 https://doi.org/10.1038/371346a0
211 rdf:type schema:CreativeWork
212 sg:pub.10.1038/ni0403-308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006069267
213 https://doi.org/10.1038/ni0403-308
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/sj.cdd.4401400 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019493693
216 https://doi.org/10.1038/sj.cdd.4401400
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/sj.leu.2404357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015388322
219 https://doi.org/10.1038/sj.leu.2404357
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/sj.onc.1201947 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018076908
222 https://doi.org/10.1038/sj.onc.1201947
223 rdf:type schema:CreativeWork
224 sg:pub.10.1186/1743-422x-3-104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043875359
225 https://doi.org/10.1186/1743-422x-3-104
226 rdf:type schema:CreativeWork
227 https://app.dimensions.ai/details/publication/pub.1077437704 schema:CreativeWork
228 https://app.dimensions.ai/details/publication/pub.1077945666 schema:CreativeWork
229 https://app.dimensions.ai/details/publication/pub.1079981956 schema:CreativeWork
230 https://app.dimensions.ai/details/publication/pub.1082689984 schema:CreativeWork
231 https://doi.org/10.1002/ijc.22024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000262085
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1002/j.1460-2075.1996.tb01045.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1083023835
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1006/viro.2001.1237 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002052911
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/0014-5793(95)00677-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035310560
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/0092-8674(94)90344-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028334480
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/j.bbrc.2007.04.115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048069924
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/j.febslet.2005.11.040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036939610
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/j.febslet.2005.12.060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052553989
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/j.semcancer.2003.10.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032956206
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/s0005-2736(96)00237-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012980274
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1016/s0005-2736(98)00014-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039049398
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1016/s0960-9822(02)00973-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024839636
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1021/bi00298a016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055169195
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1021/bi00558a001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055180713
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1021/bi00601a020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055182261
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1021/bi701920f schema:sameAs https://app.dimensions.ai/details/publication/pub.1055207971
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1021/bi9702851 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055213621
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1042/bj3150021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012695534
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1042/bj3260001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014115395
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1042/bj3350597 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044124517
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1073/pnas.84.17.6103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042356499
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1074/jbc.270.32.19041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030172522
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1080/09687680701613713 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003218678
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1083/jcb.200309101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009857792
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1084/jem.20030267 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041106320
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1086/520605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053714396
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1093/emboj/18.22.6211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012199783
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1111/j.1349-7006.2007.00606.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1027380026
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1124/mol.65.3.675 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009746539
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1126/science.1360704 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062477732
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1126/science.663661 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062639318
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1128/ec.00114-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009156224
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1128/jvi.02233-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034612310
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1128/jvi.77.12.7067-7077.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011461355
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1128/jvi.79.19.12394-12400.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014547247
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1146/annurev.bi.62.070193.002125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003490677
302 rdf:type schema:CreativeWork
303 https://doi.org/10.1152/ajpendo.1978.235.2.e97 schema:sameAs https://app.dimensions.ai/details/publication/pub.1081998480
304 rdf:type schema:CreativeWork
305 https://doi.org/10.1182/blood-2005-08-3182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050475732
306 rdf:type schema:CreativeWork
307 https://doi.org/10.1517/17425255.3.6.805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022761062
308 rdf:type schema:CreativeWork
309 https://doi.org/10.3109/09687689509038509 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011656281
310 rdf:type schema:CreativeWork
311 https://doi.org/10.3892/ijo.27.5.1329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071515872
312 rdf:type schema:CreativeWork
313 https://www.grid.ac/institutes/grid.417768.b schema:alternateName Center for Cancer Research
314 schema:name Nanobiology Program, Center of Cancer Research, National Cancer Institute, Frederick, Maryland, USA
315 rdf:type schema:Organization
316 https://www.grid.ac/institutes/grid.48336.3a schema:alternateName National Cancer Institute
317 schema:name Basic Research Program, SAIC-Frederick Inc, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA
318 Nanobiology Program, Center of Cancer Research, National Cancer Institute, Frederick, Maryland, USA
319 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...