Following damage, the majority of bone marrow-derived airway cells express an epithelial marker View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-12

AUTHORS

Heather MacPherson, Pamela A Keir, Carol J Edwards, Sheila Webb, Julia R Dorin

ABSTRACT

BACKGROUND: Adult-derived bone marrow stem cells are capable of reconstituting the haematopoietic system. However there is ongoing debate in the literature as to whether bone marrow derived cells have the ability to populate other tissues and express tissue specific markers. The airway has been an organ of major interest and was one of the first where this was demonstrated. We have previously demonstrated that the mouse airway can be repopulated by side population bone marrow transplanted cells. Here we investigate the frequency and phenotypic nature of these bone marrow derived cells. METHODS: Female mice were engrafted with male whole bone marrow or side population (SP) cells and subjected to detergent-induced damage after 3 months. Donor cells were identified by Y chromosome fluorescence in situ hybridisation and their phenotype was assessed by immunohistochemistry on the same sections. Slides were visualised by a combination of widefield and deconvolved microscopy and whole cells were analysed on cytospin preparations. RESULTS: The frequencies of engraftment of male cells in the airway of mice that show this (9/10), range from 1.0-1.6% with whole marrow and 0.6-1.5% with SP cells. Undamaged controls have only between 0.1 and 0.2% male cells in the trachea. By widefield microscopy analysis we find 60.2% (53/88) of male donor derived cells express cytokeratins as a marker of epithelial cells. These results were reinforced using deconvolved microscopy and scored by two independent investigators. In addition cytospin analysis of cells dissociated from the damaged trachea of engrafted mice also reveals donor derived Y chromosome positive cells that are immunopositive for cytokeratin. Using cytokeratin and the universal haematopoietic marker CD45 immunohistochemistry, we find the donor derived cells fall into four phenotypic classes. We do not detect cytokeratin positive cells in whole bone marrow using cytokeratin immunostaining and we do not detect any cytokeratin mRNA in SP or bone marrow samples by RT-PCR. CONCLUSION: The appearance of bone marrow derived cells in the tracheal epithelium is enriched by detergent-induced tissue damage and the majority of these cells express an epithelial marker. The cytokeratin positive donor derived cells in the tracheal epithelium are not present in the injected donor cells and must have acquired this novel phenotype in vivo. More... »

PAGES

145

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1465-9921-7-145

DOI

http://dx.doi.org/10.1186/1465-9921-7-145

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038242037

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17177981


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1102", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Cardiorespiratory Medicine and Haematology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Marrow Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Marrow Transplantation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epithelial Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leukocyte Common Antigens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Respiratory Mucosa", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trachea", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tracheal Diseases", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Western General Hospital", 
          "id": "https://www.grid.ac/institutes/grid.417068.c", 
          "name": [
            "MRC Human Genetics Unit, Western General Hospital, Crewe Road South, EH4 2XU, Edinburgh, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "MacPherson", 
        "givenName": "Heather", 
        "id": "sg:person.01023071616.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023071616.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Western General Hospital", 
          "id": "https://www.grid.ac/institutes/grid.417068.c", 
          "name": [
            "MRC Human Genetics Unit, Western General Hospital, Crewe Road South, EH4 2XU, Edinburgh, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Keir", 
        "givenName": "Pamela A", 
        "id": "sg:person.01265163030.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265163030.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Western General Hospital", 
          "id": "https://www.grid.ac/institutes/grid.417068.c", 
          "name": [
            "MRC Human Genetics Unit, Western General Hospital, Crewe Road South, EH4 2XU, Edinburgh, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Edwards", 
        "givenName": "Carol J", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Western General Hospital", 
          "id": "https://www.grid.ac/institutes/grid.417068.c", 
          "name": [
            "MRC Human Genetics Unit, Western General Hospital, Crewe Road South, EH4 2XU, Edinburgh, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Webb", 
        "givenName": "Sheila", 
        "id": "sg:person.01234307221.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234307221.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Western General Hospital", 
          "id": "https://www.grid.ac/institutes/grid.417068.c", 
          "name": [
            "MRC Human Genetics Unit, Western General Hospital, Crewe Road South, EH4 2XU, Edinburgh, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dorin", 
        "givenName": "Julia R", 
        "id": "sg:person.01053664373.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053664373.40"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1172/jci21301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001156352"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1682", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007433431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1165/ajrcmb.20.6.3475", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007784310"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(01)00328-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009943717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2184.2004.00300.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010815781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05282", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011740155", 
          "https://doi.org/10.1038/nature05282"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05282", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011740155", 
          "https://doi.org/10.1038/nature05282"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05282", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011740155", 
          "https://doi.org/10.1038/nature05282"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(82)90400-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012378105"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(87)90021-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013321498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00062752-200305000-00003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014206842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00062752-200305000-00003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014206842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00062752-200305000-00003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014206842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(02)00931-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025587809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(02)00931-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025587809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1165/rcmb.2005-0175rc", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026332134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1165/rcmb.2005-0175rc", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026332134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0508593103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027104649"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymthe.2005.09.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036917113"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02069", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037722033", 
          "https://doi.org/10.1038/nature02069"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02069", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037722033", 
          "https://doi.org/10.1038/nature02069"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/81326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039353607", 
          "https://doi.org/10.1038/81326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/81326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039353607", 
          "https://doi.org/10.1038/81326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajplung.00006.2006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040453106"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.02375", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040980756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1164/rccm.200502-309oc", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042877223"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0510758103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043228323"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.183.4.1797", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044033062"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1165/rcmb.2005-0332oc", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044304579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1165/rcmb.2005-0332oc", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044304579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1165/ajrcmb.24.6.4217", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044521169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm963", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047367124", 
          "https://doi.org/10.1038/nm963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm963", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047367124", 
          "https://doi.org/10.1038/nm963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1165/rcmb.2005-0129oc", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049086465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1165/rcmb.2005-0129oc", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049086465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01531", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053292933", 
          "https://doi.org/10.1038/nature01531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01531", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053292933", 
          "https://doi.org/10.1038/nature01531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1098925", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062449702"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.305.5680.27a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062585384"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci15182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063415340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci18847", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063416475"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajplung.2000.279.4.l766", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074701860"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074963614", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082983974", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-12", 
    "datePublishedReg": "2006-12-01", 
    "description": "BACKGROUND: Adult-derived bone marrow stem cells are capable of reconstituting the haematopoietic system. However there is ongoing debate in the literature as to whether bone marrow derived cells have the ability to populate other tissues and express tissue specific markers. The airway has been an organ of major interest and was one of the first where this was demonstrated. We have previously demonstrated that the mouse airway can be repopulated by side population bone marrow transplanted cells. Here we investigate the frequency and phenotypic nature of these bone marrow derived cells.\nMETHODS: Female mice were engrafted with male whole bone marrow or side population (SP) cells and subjected to detergent-induced damage after 3 months. Donor cells were identified by Y chromosome fluorescence in situ hybridisation and their phenotype was assessed by immunohistochemistry on the same sections. Slides were visualised by a combination of widefield and deconvolved microscopy and whole cells were analysed on cytospin preparations.\nRESULTS: The frequencies of engraftment of male cells in the airway of mice that show this (9/10), range from 1.0-1.6% with whole marrow and 0.6-1.5% with SP cells. Undamaged controls have only between 0.1 and 0.2% male cells in the trachea. By widefield microscopy analysis we find 60.2% (53/88) of male donor derived cells express cytokeratins as a marker of epithelial cells. These results were reinforced using deconvolved microscopy and scored by two independent investigators. In addition cytospin analysis of cells dissociated from the damaged trachea of engrafted mice also reveals donor derived Y chromosome positive cells that are immunopositive for cytokeratin. Using cytokeratin and the universal haematopoietic marker CD45 immunohistochemistry, we find the donor derived cells fall into four phenotypic classes. We do not detect cytokeratin positive cells in whole bone marrow using cytokeratin immunostaining and we do not detect any cytokeratin mRNA in SP or bone marrow samples by RT-PCR.\nCONCLUSION: The appearance of bone marrow derived cells in the tracheal epithelium is enriched by detergent-induced tissue damage and the majority of these cells express an epithelial marker. The cytokeratin positive donor derived cells in the tracheal epithelium are not present in the injected donor cells and must have acquired this novel phenotype in vivo.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1465-9921-7-145", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2752717", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1028525", 
        "issn": [
          "1465-9921", 
          "1465-993X"
        ], 
        "name": "Respiratory Research", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "Following damage, the majority of bone marrow-derived airway cells express an epithelial marker", 
    "pagination": "145", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b62c38d436eae4f9edd0ab884ab7ce1377b7e4fba9907891ce8060a3016fecfd"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17177981"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101090633"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1465-9921-7-145"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1038242037"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1465-9921-7-145", 
      "https://app.dimensions.ai/details/publication/pub.1038242037"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T01:58", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000506.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186/1465-9921-7-145"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1465-9921-7-145'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1465-9921-7-145'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1465-9921-7-145'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1465-9921-7-145'


 

This table displays all metadata directly associated to this object as RDF triples.

253 TRIPLES      21 PREDICATES      75 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1465-9921-7-145 schema:about N0d866cff2c7547d081f450f4edb768a7
2 N0eefbd2baaa44c19ba19e2639c8b5069
3 N2806fb74273349ea92cea4fb0f826652
4 N49b7b6bc90594493bc7854ce60a18e6a
5 N761ab5e78f334c498418c02c843cc1d8
6 N8dc5c0a0ca8444d3a8aa59e20228384f
7 Na8240ded7751430a9ecaf7b10f16d648
8 Naed4ee05b3704f38b41f4ac30fab360f
9 Nb10e4e70734f4a7b828c25978ce2388d
10 Nb4da8daa767d4e4480d25482f0093dac
11 Nb55aebbfa1cc47abb127931ae0b3172e
12 Ne276fa727f7241b6aeb9e37cec2e5e43
13 Ne918884d419b4db6b67f4bb9e80e8223
14 Nfa508907cd6a41efa8b75b14ac6c6e97
15 anzsrc-for:11
16 anzsrc-for:1102
17 schema:author Na7069c96d48e4093990a817ef1bde31b
18 schema:citation sg:pub.10.1038/81326
19 sg:pub.10.1038/nature01531
20 sg:pub.10.1038/nature02069
21 sg:pub.10.1038/nature05282
22 sg:pub.10.1038/nm963
23 https://app.dimensions.ai/details/publication/pub.1074963614
24 https://app.dimensions.ai/details/publication/pub.1082983974
25 https://doi.org/10.1002/path.1682
26 https://doi.org/10.1016/0003-2697(87)90021-2
27 https://doi.org/10.1016/0092-8674(82)90400-7
28 https://doi.org/10.1016/j.ymthe.2005.09.004
29 https://doi.org/10.1016/s0092-8674(01)00328-2
30 https://doi.org/10.1016/s0301-472x(02)00931-1
31 https://doi.org/10.1073/pnas.0508593103
32 https://doi.org/10.1073/pnas.0510758103
33 https://doi.org/10.1084/jem.183.4.1797
34 https://doi.org/10.1097/00062752-200305000-00003
35 https://doi.org/10.1111/j.1365-2184.2004.00300.x
36 https://doi.org/10.1126/science.1098925
37 https://doi.org/10.1126/science.305.5680.27a
38 https://doi.org/10.1152/ajplung.00006.2006
39 https://doi.org/10.1152/ajplung.2000.279.4.l766
40 https://doi.org/10.1164/rccm.200502-309oc
41 https://doi.org/10.1165/ajrcmb.20.6.3475
42 https://doi.org/10.1165/ajrcmb.24.6.4217
43 https://doi.org/10.1165/rcmb.2005-0129oc
44 https://doi.org/10.1165/rcmb.2005-0175rc
45 https://doi.org/10.1165/rcmb.2005-0332oc
46 https://doi.org/10.1172/jci15182
47 https://doi.org/10.1172/jci18847
48 https://doi.org/10.1172/jci21301
49 https://doi.org/10.1242/jcs.02375
50 schema:datePublished 2006-12
51 schema:datePublishedReg 2006-12-01
52 schema:description BACKGROUND: Adult-derived bone marrow stem cells are capable of reconstituting the haematopoietic system. However there is ongoing debate in the literature as to whether bone marrow derived cells have the ability to populate other tissues and express tissue specific markers. The airway has been an organ of major interest and was one of the first where this was demonstrated. We have previously demonstrated that the mouse airway can be repopulated by side population bone marrow transplanted cells. Here we investigate the frequency and phenotypic nature of these bone marrow derived cells. METHODS: Female mice were engrafted with male whole bone marrow or side population (SP) cells and subjected to detergent-induced damage after 3 months. Donor cells were identified by Y chromosome fluorescence in situ hybridisation and their phenotype was assessed by immunohistochemistry on the same sections. Slides were visualised by a combination of widefield and deconvolved microscopy and whole cells were analysed on cytospin preparations. RESULTS: The frequencies of engraftment of male cells in the airway of mice that show this (9/10), range from 1.0-1.6% with whole marrow and 0.6-1.5% with SP cells. Undamaged controls have only between 0.1 and 0.2% male cells in the trachea. By widefield microscopy analysis we find 60.2% (53/88) of male donor derived cells express cytokeratins as a marker of epithelial cells. These results were reinforced using deconvolved microscopy and scored by two independent investigators. In addition cytospin analysis of cells dissociated from the damaged trachea of engrafted mice also reveals donor derived Y chromosome positive cells that are immunopositive for cytokeratin. Using cytokeratin and the universal haematopoietic marker CD45 immunohistochemistry, we find the donor derived cells fall into four phenotypic classes. We do not detect cytokeratin positive cells in whole bone marrow using cytokeratin immunostaining and we do not detect any cytokeratin mRNA in SP or bone marrow samples by RT-PCR. CONCLUSION: The appearance of bone marrow derived cells in the tracheal epithelium is enriched by detergent-induced tissue damage and the majority of these cells express an epithelial marker. The cytokeratin positive donor derived cells in the tracheal epithelium are not present in the injected donor cells and must have acquired this novel phenotype in vivo.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree true
56 schema:isPartOf Ncfd35db9109a4463b2f5a929e3c57d63
57 Nf1f843ea32034435b418b4ccd28e6ca0
58 sg:journal.1028525
59 schema:name Following damage, the majority of bone marrow-derived airway cells express an epithelial marker
60 schema:pagination 145
61 schema:productId N2b69d657decd4b449a0f5016c4ea1ce4
62 N872f4aceab2f408a96929228659e5fd9
63 N9f1a25d890bb4b6fa205bb64fe3490a8
64 Nba55138a406b4b7d968a0ea2963301d8
65 Nd6e878fd87c84f70860c88cb859799d6
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038242037
67 https://doi.org/10.1186/1465-9921-7-145
68 schema:sdDatePublished 2019-04-11T01:58
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher Nca918d2c3b0a41829c7df6642dbe610b
71 schema:url http://link.springer.com/10.1186/1465-9921-7-145
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N0d866cff2c7547d081f450f4edb768a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Cells, Cultured
77 rdf:type schema:DefinedTerm
78 N0eefbd2baaa44c19ba19e2639c8b5069 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Tracheal Diseases
80 rdf:type schema:DefinedTerm
81 N2806fb74273349ea92cea4fb0f826652 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Mice
83 rdf:type schema:DefinedTerm
84 N2b69d657decd4b449a0f5016c4ea1ce4 schema:name pubmed_id
85 schema:value 17177981
86 rdf:type schema:PropertyValue
87 N36e0674681054a768a39911ccc4c886f rdf:first sg:person.01053664373.40
88 rdf:rest rdf:nil
89 N49b7b6bc90594493bc7854ce60a18e6a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Respiratory Mucosa
91 rdf:type schema:DefinedTerm
92 N4f447ae8ff43489496595bebaa3dd9c2 rdf:first sg:person.01265163030.93
93 rdf:rest Nd859f361c9354c4094f60d6afce18fe5
94 N761ab5e78f334c498418c02c843cc1d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Bone Marrow Transplantation
96 rdf:type schema:DefinedTerm
97 N872f4aceab2f408a96929228659e5fd9 schema:name readcube_id
98 schema:value b62c38d436eae4f9edd0ab884ab7ce1377b7e4fba9907891ce8060a3016fecfd
99 rdf:type schema:PropertyValue
100 N8dc5c0a0ca8444d3a8aa59e20228384f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Female
102 rdf:type schema:DefinedTerm
103 N8f4157c912c845dcb170e8217ef9bdfe schema:affiliation https://www.grid.ac/institutes/grid.417068.c
104 schema:familyName Edwards
105 schema:givenName Carol J
106 rdf:type schema:Person
107 N9f1a25d890bb4b6fa205bb64fe3490a8 schema:name dimensions_id
108 schema:value pub.1038242037
109 rdf:type schema:PropertyValue
110 Na7069c96d48e4093990a817ef1bde31b rdf:first sg:person.01023071616.54
111 rdf:rest N4f447ae8ff43489496595bebaa3dd9c2
112 Na8240ded7751430a9ecaf7b10f16d648 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Leukocyte Common Antigens
114 rdf:type schema:DefinedTerm
115 Naed4ee05b3704f38b41f4ac30fab360f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Epithelial Cells
117 rdf:type schema:DefinedTerm
118 Nb10e4e70734f4a7b828c25978ce2388d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Cell Differentiation
120 rdf:type schema:DefinedTerm
121 Nb4da8daa767d4e4480d25482f0093dac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Male
123 rdf:type schema:DefinedTerm
124 Nb55aebbfa1cc47abb127931ae0b3172e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Bone Marrow Cells
126 rdf:type schema:DefinedTerm
127 Nba55138a406b4b7d968a0ea2963301d8 schema:name doi
128 schema:value 10.1186/1465-9921-7-145
129 rdf:type schema:PropertyValue
130 Nca918d2c3b0a41829c7df6642dbe610b schema:name Springer Nature - SN SciGraph project
131 rdf:type schema:Organization
132 Ncfd35db9109a4463b2f5a929e3c57d63 schema:issueNumber 1
133 rdf:type schema:PublicationIssue
134 Nd6e878fd87c84f70860c88cb859799d6 schema:name nlm_unique_id
135 schema:value 101090633
136 rdf:type schema:PropertyValue
137 Nd859f361c9354c4094f60d6afce18fe5 rdf:first N8f4157c912c845dcb170e8217ef9bdfe
138 rdf:rest Nfc2daafc564f445bae89ad3e22da5f7f
139 Ne276fa727f7241b6aeb9e37cec2e5e43 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Animals
141 rdf:type schema:DefinedTerm
142 Ne918884d419b4db6b67f4bb9e80e8223 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Trachea
144 rdf:type schema:DefinedTerm
145 Nf1f843ea32034435b418b4ccd28e6ca0 schema:volumeNumber 7
146 rdf:type schema:PublicationVolume
147 Nfa508907cd6a41efa8b75b14ac6c6e97 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Biomarkers
149 rdf:type schema:DefinedTerm
150 Nfc2daafc564f445bae89ad3e22da5f7f rdf:first sg:person.01234307221.28
151 rdf:rest N36e0674681054a768a39911ccc4c886f
152 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
153 schema:name Medical and Health Sciences
154 rdf:type schema:DefinedTerm
155 anzsrc-for:1102 schema:inDefinedTermSet anzsrc-for:
156 schema:name Cardiorespiratory Medicine and Haematology
157 rdf:type schema:DefinedTerm
158 sg:grant.2752717 http://pending.schema.org/fundedItem sg:pub.10.1186/1465-9921-7-145
159 rdf:type schema:MonetaryGrant
160 sg:journal.1028525 schema:issn 1465-9921
161 1465-993X
162 schema:name Respiratory Research
163 rdf:type schema:Periodical
164 sg:person.01023071616.54 schema:affiliation https://www.grid.ac/institutes/grid.417068.c
165 schema:familyName MacPherson
166 schema:givenName Heather
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023071616.54
168 rdf:type schema:Person
169 sg:person.01053664373.40 schema:affiliation https://www.grid.ac/institutes/grid.417068.c
170 schema:familyName Dorin
171 schema:givenName Julia R
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053664373.40
173 rdf:type schema:Person
174 sg:person.01234307221.28 schema:affiliation https://www.grid.ac/institutes/grid.417068.c
175 schema:familyName Webb
176 schema:givenName Sheila
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234307221.28
178 rdf:type schema:Person
179 sg:person.01265163030.93 schema:affiliation https://www.grid.ac/institutes/grid.417068.c
180 schema:familyName Keir
181 schema:givenName Pamela A
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265163030.93
183 rdf:type schema:Person
184 sg:pub.10.1038/81326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039353607
185 https://doi.org/10.1038/81326
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/nature01531 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053292933
188 https://doi.org/10.1038/nature01531
189 rdf:type schema:CreativeWork
190 sg:pub.10.1038/nature02069 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037722033
191 https://doi.org/10.1038/nature02069
192 rdf:type schema:CreativeWork
193 sg:pub.10.1038/nature05282 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011740155
194 https://doi.org/10.1038/nature05282
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/nm963 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047367124
197 https://doi.org/10.1038/nm963
198 rdf:type schema:CreativeWork
199 https://app.dimensions.ai/details/publication/pub.1074963614 schema:CreativeWork
200 https://app.dimensions.ai/details/publication/pub.1082983974 schema:CreativeWork
201 https://doi.org/10.1002/path.1682 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007433431
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/0003-2697(87)90021-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013321498
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/0092-8674(82)90400-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012378105
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/j.ymthe.2005.09.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036917113
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/s0092-8674(01)00328-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009943717
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/s0301-472x(02)00931-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025587809
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1073/pnas.0508593103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027104649
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1073/pnas.0510758103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043228323
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1084/jem.183.4.1797 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044033062
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1097/00062752-200305000-00003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014206842
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1111/j.1365-2184.2004.00300.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010815781
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1126/science.1098925 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062449702
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1126/science.305.5680.27a schema:sameAs https://app.dimensions.ai/details/publication/pub.1062585384
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1152/ajplung.00006.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040453106
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1152/ajplung.2000.279.4.l766 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074701860
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1164/rccm.200502-309oc schema:sameAs https://app.dimensions.ai/details/publication/pub.1042877223
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1165/ajrcmb.20.6.3475 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007784310
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1165/ajrcmb.24.6.4217 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044521169
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1165/rcmb.2005-0129oc schema:sameAs https://app.dimensions.ai/details/publication/pub.1049086465
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1165/rcmb.2005-0175rc schema:sameAs https://app.dimensions.ai/details/publication/pub.1026332134
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1165/rcmb.2005-0332oc schema:sameAs https://app.dimensions.ai/details/publication/pub.1044304579
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1172/jci15182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063415340
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1172/jci18847 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063416475
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1172/jci21301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001156352
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1242/jcs.02375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040980756
250 rdf:type schema:CreativeWork
251 https://www.grid.ac/institutes/grid.417068.c schema:alternateName Western General Hospital
252 schema:name MRC Human Genetics Unit, Western General Hospital, Crewe Road South, EH4 2XU, Edinburgh, UK
253 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...