CD8 positive T cells express IL-17 in patients with chronic obstructive pulmonary disease View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-12

AUTHORS

Ying Chang, Jessica Nadigel, Nicholas Boulais, Jean Bourbeau, François Maltais, David H Eidelman, Qutayba Hamid

ABSTRACT

BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a progressive and irreversible chronic inflammatory disease of the lung. The nature of the immune reaction in COPD raises the possibility that IL-17 and related cytokines may contribute to this disorder. This study analyzed the expression of IL-17A and IL-17F as well as the phenotype of cells producing them in bronchial biopsies from COPD patients. METHODS: Bronchoscopic biopsies of the airway were obtained from 16 COPD subjects (GOLD stage 1-4) and 15 control subjects. Paraffin sections were used for the investigation of IL-17A and IL-17F expression in the airways by immunohistochemistry, and frozen sections were used for the immunofluorescence double staining of IL-17A or IL-17F paired with CD4 or CD8. In order to confirm the expression of IL-17A and IL-17F at the mRNA level, a quantitative RT-PCR was performed on the total mRNA extracted from entire section or CD8 positive cells selected by laser capture microdissection. RESULTS: IL-17F immunoreactivity was significantly higher in the bronchial biopsies of COPD patients compared to control subjects (P < 0.0001). In the submucosa, the absolute number of both IL-17A and IL-17F positive cells was higher in COPD patients (P < 0.0001). After adjusting for the total number of cells in the submucosa, we still found that more cells were positive for both IL-17A (P < 0.0001) and IL-17F (P < 0.0001) in COPD patients compared to controls. The mRNA expression of IL-17A and IL-17F in airways of COPD patients was confirmed by RT-PCR. The expression of IL-17A and IL-17F was co-localized with not only CD4 but also CD8, which was further confirmed by RT-PCR on laser capture microdissection selected CD8 positive cells. CONCLUSION: These findings support the notion that Th17 cytokines could play important roles in the pathogenesis of COPD, raising the possibility of using this mechanism as the basis for novel therapeutic approaches. More... »

PAGES

43

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1465-9921-12-43

DOI

http://dx.doi.org/10.1186/1465-9921-12-43

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007667064

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21477350


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RDF/XML is a standard XML format for linked data.

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297 https://www.grid.ac/institutes/grid.63984.30 schema:alternateName McGill University Health Centre
298 schema:name Respiratory Division, Research Institute of McGill University Health Centre, 2155 Guy Street, Suite 900, H3H 2R9, Montreal, QC, Canada
299 rdf:type schema:Organization
 




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