Diminished Ovarian Reserve Chemotherapy-Induced Mouse Model: A Tool for the Preclinical Assessment of New Therapies for Ovarian Damage. View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-02-21

AUTHORS

Anna Buigues, Maria Marchante, Sonia Herraiz, Antonio Pellicer

ABSTRACT

Diminished ovarian reserve (DOR) and primary ovarian insufficiency (POI) are primary factors leading to infertility. However, there is a lack of appropriate animal models of DOR usable for assessing new therapeutic strategies. In this study, we aimed to evaluate whether chemotherapy treatment in mice could reproduce features similar of that observed in women with DOR. Twenty-one Nonobese diabetic/severe combined immunodeficiency (NOD/SCID) female mice were allocated to 3 groups (n = 7/group): control, single dose of vehicle (Dimethyl Sulfoxide [DMSO]); DOR, single reduced chemotherapy dose; and POI, single standard chemotherapy dose. After 21 days, mice underwent ovarian hyperstimulation and mating. Part of the animals were harvested to analyze ovarian reserve, ovulation and fertilization rates, and morphology, apoptosis, and vascularization of the ovarian stroma. The remaining mice underwent multiple matings to assess pregnancy rates and litter sizes. The DOR and POI mice showed an impaired estrous cyclicity and a decrease in ovarian mass, number of follicles, Metaphase II (MII) oocytes, and embryos as well as in ovarian stroma vascularization. Mice in both models showed also an increase in the percentage of morphologically abnormal follicles, stromal degeneration, and apoptosis. Similar to that observed in DOR and POI patients, these impairments were less severe in DOR than in POI mice. None of the POI females were able to achieve a pregnancy. Meanwhile, DOR females achieved several consecutive pregnancies, although litter size was decreased when compared to controls. In conclusion, a mouse model which displayed most of the ovarian characteristics and fertility outcomes of women with DOR has been established using a single dose of chemotherapy. More... »

PAGES

1933719119831784

Identifiers

URI

http://scigraph.springernature.com/pub.10.1177/1933719119831784

DOI

http://dx.doi.org/10.1177/1933719119831784

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112291919

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30791852


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1114", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Paediatrics and Reproductive Medicine", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "2 Department of Pediatrics, Obstetrics and Gynecology, School of Medicine, Valencia University, Valencia, Spain.", 
          "id": "http://www.grid.ac/institutes/grid.5338.d", 
          "name": [
            "1 IVI Foundation, Valencia Spain.", 
            "2 Department of Pediatrics, Obstetrics and Gynecology, School of Medicine, Valencia University, Valencia, Spain."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Buigues", 
        "givenName": "Anna", 
        "id": "sg:person.011724070757.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011724070757.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "2 Department of Pediatrics, Obstetrics and Gynecology, School of Medicine, Valencia University, Valencia, Spain.", 
          "id": "http://www.grid.ac/institutes/grid.5338.d", 
          "name": [
            "1 IVI Foundation, Valencia Spain.", 
            "2 Department of Pediatrics, Obstetrics and Gynecology, School of Medicine, Valencia University, Valencia, Spain."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Marchante", 
        "givenName": "Maria", 
        "id": "sg:person.010363147373.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010363147373.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "4 IVI-RMA Valencia, Valencia, Spain.", 
          "id": "http://www.grid.ac/institutes/grid.419275.c", 
          "name": [
            "1 IVI Foundation, Valencia Spain.", 
            "3 Reproductive Medicine Research Group, IIS La Fe, Valencia, Spain.", 
            "4 IVI-RMA Valencia, Valencia, Spain."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Herraiz", 
        "givenName": "Sonia", 
        "id": "sg:person.0725244145.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725244145.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "5 IVI-RMA Rome, Rome, Italy.", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "1 IVI Foundation, Valencia Spain.", 
            "3 Reproductive Medicine Research Group, IIS La Fe, Valencia, Spain.", 
            "5 IVI-RMA Rome, Rome, Italy."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pellicer", 
        "givenName": "Antonio", 
        "id": "sg:person.01125174440.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125174440.05"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1631/jzus.b1200181", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050882124", 
          "https://doi.org/10.1631/jzus.b1200181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04929", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009505802", 
          "https://doi.org/10.1038/nature04929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10815-015-0595-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036792268", 
          "https://doi.org/10.1007/s10815-015-0595-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bmt.1702695", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048075300", 
          "https://doi.org/10.1038/sj.bmt.1702695"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02316", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031245923", 
          "https://doi.org/10.1038/nature02316"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-02-21", 
    "datePublishedReg": "2019-02-21", 
    "description": "Diminished ovarian reserve (DOR) and primary ovarian insufficiency (POI) are primary factors leading to infertility. However, there is a lack of appropriate animal models of DOR usable for assessing new therapeutic strategies. In this study, we aimed to evaluate whether chemotherapy treatment in mice could reproduce features similar of that observed in women with DOR. Twenty-one Nonobese diabetic/severe combined immunodeficiency (NOD/SCID) female mice were allocated to 3 groups (n = 7/group): control, single dose of vehicle (Dimethyl Sulfoxide [DMSO]); DOR, single reduced chemotherapy dose; and POI, single standard chemotherapy dose. After 21 days, mice underwent ovarian hyperstimulation and mating. Part of the animals were harvested to analyze ovarian reserve, ovulation and fertilization rates, and morphology, apoptosis, and vascularization of the ovarian stroma. The remaining mice underwent multiple matings to assess pregnancy rates and litter sizes. The DOR and POI mice showed an impaired estrous cyclicity and a decrease in ovarian mass, number of follicles, Metaphase II (MII) oocytes, and embryos as well as in ovarian stroma vascularization. Mice in both models showed also an increase in the percentage of morphologically abnormal follicles, stromal degeneration, and apoptosis. Similar to that observed in DOR and POI patients, these impairments were less severe in DOR than in POI mice. None of the POI females were able to achieve a pregnancy. Meanwhile, DOR females achieved several consecutive pregnancies, although litter size was decreased when compared to controls. In conclusion, a mouse model which displayed most of the ovarian characteristics and fertility outcomes of women with DOR has been established using a single dose of chemotherapy.", 
    "genre": "article", 
    "id": "sg:pub.10.1177/1933719119831784", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1112196", 
        "issn": [
          "1933-7191", 
          "1933-7205"
        ], 
        "name": "Reproductive Sciences", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }
    ], 
    "keywords": [
      "primary ovarian insufficiency", 
      "POI mice", 
      "ovarian reserve", 
      "chemotherapy dose", 
      "single dose", 
      "mouse model", 
      "number of follicles", 
      "appropriate animal models", 
      "nonobese diabetic/", 
      "new therapeutic strategies", 
      "metaphase II oocytes", 
      "stromal degeneration", 
      "ovarian hyperstimulation", 
      "ovarian damage", 
      "ovarian masses", 
      "POI patients", 
      "ovarian stroma", 
      "consecutive pregnancies", 
      "chemotherapy treatment", 
      "abnormal follicles", 
      "female mice", 
      "pregnancy rate", 
      "estrous cyclicity", 
      "ovarian insufficiency", 
      "diabetic/", 
      "therapeutic strategies", 
      "preclinical assessment", 
      "new therapies", 
      "ovarian characteristics", 
      "animal models", 
      "fertility outcomes", 
      "litter size", 
      "mice", 
      "dose", 
      "pregnancy", 
      "fertilization rate", 
      "follicles", 
      "women", 
      "vascularization", 
      "DOR", 
      "apoptosis", 
      "females", 
      "hyperstimulation", 
      "chemotherapy", 
      "patients", 
      "infertility", 
      "therapy", 
      "insufficiency", 
      "ovulation", 
      "degeneration", 
      "stroma", 
      "impairment", 
      "outcomes", 
      "treatment", 
      "control", 
      "days", 
      "animals", 
      "oocytes", 
      "rate", 
      "conclusion", 
      "group", 
      "percentage", 
      "damage", 
      "assessment", 
      "factors", 
      "decrease", 
      "study", 
      "primary factor", 
      "increase", 
      "lack", 
      "embryos", 
      "reserves", 
      "cyclicity", 
      "mating", 
      "mass", 
      "strategies", 
      "model", 
      "number", 
      "size", 
      "features", 
      "characteristics", 
      "part", 
      "tool", 
      "morphology", 
      "vehicles", 
      "multiple mating"
    ], 
    "name": "Diminished Ovarian Reserve Chemotherapy-Induced Mouse Model: A Tool for the Preclinical Assessment of New Therapies for Ovarian Damage.", 
    "pagination": "1933719119831784", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112291919"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1177/1933719119831784"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30791852"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1177/1933719119831784", 
      "https://app.dimensions.ai/details/publication/pub.1112291919"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:36", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_819.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1177/1933719119831784"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1177/1933719119831784'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1177/1933719119831784'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1177/1933719119831784'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1177/1933719119831784'


 

This table displays all metadata directly associated to this object as RDF triples.

198 TRIPLES      22 PREDICATES      116 URIs      102 LITERALS      5 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1177/1933719119831784 schema:about anzsrc-for:11
2 anzsrc-for:1112
3 anzsrc-for:1114
4 schema:author N298e0ba3928346da97dc3a7dcaba824a
5 schema:citation sg:pub.10.1007/s10815-015-0595-y
6 sg:pub.10.1038/nature02316
7 sg:pub.10.1038/nature04929
8 sg:pub.10.1038/sj.bmt.1702695
9 sg:pub.10.1631/jzus.b1200181
10 schema:datePublished 2019-02-21
11 schema:datePublishedReg 2019-02-21
12 schema:description Diminished ovarian reserve (DOR) and primary ovarian insufficiency (POI) are primary factors leading to infertility. However, there is a lack of appropriate animal models of DOR usable for assessing new therapeutic strategies. In this study, we aimed to evaluate whether chemotherapy treatment in mice could reproduce features similar of that observed in women with DOR. Twenty-one Nonobese diabetic/severe combined immunodeficiency (NOD/SCID) female mice were allocated to 3 groups (n = 7/group): control, single dose of vehicle (Dimethyl Sulfoxide [DMSO]); DOR, single reduced chemotherapy dose; and POI, single standard chemotherapy dose. After 21 days, mice underwent ovarian hyperstimulation and mating. Part of the animals were harvested to analyze ovarian reserve, ovulation and fertilization rates, and morphology, apoptosis, and vascularization of the ovarian stroma. The remaining mice underwent multiple matings to assess pregnancy rates and litter sizes. The DOR and POI mice showed an impaired estrous cyclicity and a decrease in ovarian mass, number of follicles, Metaphase II (MII) oocytes, and embryos as well as in ovarian stroma vascularization. Mice in both models showed also an increase in the percentage of morphologically abnormal follicles, stromal degeneration, and apoptosis. Similar to that observed in DOR and POI patients, these impairments were less severe in DOR than in POI mice. None of the POI females were able to achieve a pregnancy. Meanwhile, DOR females achieved several consecutive pregnancies, although litter size was decreased when compared to controls. In conclusion, a mouse model which displayed most of the ovarian characteristics and fertility outcomes of women with DOR has been established using a single dose of chemotherapy.
13 schema:genre article
14 schema:inLanguage en
15 schema:isAccessibleForFree false
16 schema:isPartOf sg:journal.1112196
17 schema:keywords DOR
18 POI mice
19 POI patients
20 abnormal follicles
21 animal models
22 animals
23 apoptosis
24 appropriate animal models
25 assessment
26 characteristics
27 chemotherapy
28 chemotherapy dose
29 chemotherapy treatment
30 conclusion
31 consecutive pregnancies
32 control
33 cyclicity
34 damage
35 days
36 decrease
37 degeneration
38 diabetic/
39 dose
40 embryos
41 estrous cyclicity
42 factors
43 features
44 female mice
45 females
46 fertility outcomes
47 fertilization rate
48 follicles
49 group
50 hyperstimulation
51 impairment
52 increase
53 infertility
54 insufficiency
55 lack
56 litter size
57 mass
58 mating
59 metaphase II oocytes
60 mice
61 model
62 morphology
63 mouse model
64 multiple mating
65 new therapeutic strategies
66 new therapies
67 nonobese diabetic/
68 number
69 number of follicles
70 oocytes
71 outcomes
72 ovarian characteristics
73 ovarian damage
74 ovarian hyperstimulation
75 ovarian insufficiency
76 ovarian masses
77 ovarian reserve
78 ovarian stroma
79 ovulation
80 part
81 patients
82 percentage
83 preclinical assessment
84 pregnancy
85 pregnancy rate
86 primary factor
87 primary ovarian insufficiency
88 rate
89 reserves
90 single dose
91 size
92 strategies
93 stroma
94 stromal degeneration
95 study
96 therapeutic strategies
97 therapy
98 tool
99 treatment
100 vascularization
101 vehicles
102 women
103 schema:name Diminished Ovarian Reserve Chemotherapy-Induced Mouse Model: A Tool for the Preclinical Assessment of New Therapies for Ovarian Damage.
104 schema:pagination 1933719119831784
105 schema:productId N4e09e0a735a348e9b2f5286682ae9c3e
106 N51375b0d58474b34b2c04be60809adb2
107 Nb9922d757b394fef87619d52a27738c8
108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112291919
109 https://doi.org/10.1177/1933719119831784
110 schema:sdDatePublished 2022-05-20T07:36
111 schema:sdLicense https://scigraph.springernature.com/explorer/license/
112 schema:sdPublisher N8188808e15744c759bb4cbd5acea82e8
113 schema:url https://doi.org/10.1177/1933719119831784
114 sgo:license sg:explorer/license/
115 sgo:sdDataset articles
116 rdf:type schema:ScholarlyArticle
117 N298e0ba3928346da97dc3a7dcaba824a rdf:first sg:person.011724070757.34
118 rdf:rest Nb3b0e59f801c433ea68c026bc19d0d7a
119 N36b299d11e674a9583a845eeebebda91 rdf:first sg:person.01125174440.05
120 rdf:rest rdf:nil
121 N4e09e0a735a348e9b2f5286682ae9c3e schema:name pubmed_id
122 schema:value 30791852
123 rdf:type schema:PropertyValue
124 N51375b0d58474b34b2c04be60809adb2 schema:name dimensions_id
125 schema:value pub.1112291919
126 rdf:type schema:PropertyValue
127 N8188808e15744c759bb4cbd5acea82e8 schema:name Springer Nature - SN SciGraph project
128 rdf:type schema:Organization
129 Nb3b0e59f801c433ea68c026bc19d0d7a rdf:first sg:person.010363147373.02
130 rdf:rest Nc748752437b44f0a9b100860c711d35f
131 Nb9922d757b394fef87619d52a27738c8 schema:name doi
132 schema:value 10.1177/1933719119831784
133 rdf:type schema:PropertyValue
134 Nc748752437b44f0a9b100860c711d35f rdf:first sg:person.0725244145.42
135 rdf:rest N36b299d11e674a9583a845eeebebda91
136 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
137 schema:name Medical and Health Sciences
138 rdf:type schema:DefinedTerm
139 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
140 schema:name Oncology and Carcinogenesis
141 rdf:type schema:DefinedTerm
142 anzsrc-for:1114 schema:inDefinedTermSet anzsrc-for:
143 schema:name Paediatrics and Reproductive Medicine
144 rdf:type schema:DefinedTerm
145 sg:journal.1112196 schema:issn 1933-7191
146 1933-7205
147 schema:name Reproductive Sciences
148 schema:publisher Springer Nature
149 rdf:type schema:Periodical
150 sg:person.010363147373.02 schema:affiliation grid-institutes:grid.5338.d
151 schema:familyName Marchante
152 schema:givenName Maria
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010363147373.02
154 rdf:type schema:Person
155 sg:person.01125174440.05 schema:affiliation grid-institutes:None
156 schema:familyName Pellicer
157 schema:givenName Antonio
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125174440.05
159 rdf:type schema:Person
160 sg:person.011724070757.34 schema:affiliation grid-institutes:grid.5338.d
161 schema:familyName Buigues
162 schema:givenName Anna
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011724070757.34
164 rdf:type schema:Person
165 sg:person.0725244145.42 schema:affiliation grid-institutes:grid.419275.c
166 schema:familyName Herraiz
167 schema:givenName Sonia
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725244145.42
169 rdf:type schema:Person
170 sg:pub.10.1007/s10815-015-0595-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1036792268
171 https://doi.org/10.1007/s10815-015-0595-y
172 rdf:type schema:CreativeWork
173 sg:pub.10.1038/nature02316 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031245923
174 https://doi.org/10.1038/nature02316
175 rdf:type schema:CreativeWork
176 sg:pub.10.1038/nature04929 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009505802
177 https://doi.org/10.1038/nature04929
178 rdf:type schema:CreativeWork
179 sg:pub.10.1038/sj.bmt.1702695 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048075300
180 https://doi.org/10.1038/sj.bmt.1702695
181 rdf:type schema:CreativeWork
182 sg:pub.10.1631/jzus.b1200181 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050882124
183 https://doi.org/10.1631/jzus.b1200181
184 rdf:type schema:CreativeWork
185 grid-institutes:None schema:alternateName 5 IVI-RMA Rome, Rome, Italy.
186 schema:name 1 IVI Foundation, Valencia Spain.
187 3 Reproductive Medicine Research Group, IIS La Fe, Valencia, Spain.
188 5 IVI-RMA Rome, Rome, Italy.
189 rdf:type schema:Organization
190 grid-institutes:grid.419275.c schema:alternateName 4 IVI-RMA Valencia, Valencia, Spain.
191 schema:name 1 IVI Foundation, Valencia Spain.
192 3 Reproductive Medicine Research Group, IIS La Fe, Valencia, Spain.
193 4 IVI-RMA Valencia, Valencia, Spain.
194 rdf:type schema:Organization
195 grid-institutes:grid.5338.d schema:alternateName 2 Department of Pediatrics, Obstetrics and Gynecology, School of Medicine, Valencia University, Valencia, Spain.
196 schema:name 1 IVI Foundation, Valencia Spain.
197 2 Department of Pediatrics, Obstetrics and Gynecology, School of Medicine, Valencia University, Valencia, Spain.
198 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...