Effect of the Mobile Phase Composition on Selectivity in Supercritical Fluid Chromatography in the Separation of Salbutamol Enantiomers View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-12

AUTHORS

M. O. Kostenko, K. B. Ustinovich, O. I. Pokrovskiy, O. O. Parenago, N. G. Bazarnova, V. V. Lunin

ABSTRACT

Three chiral chromatography stationary phases based on amylose and cellulose were tested for the supercritical fluid chromatography separation of antiasthmatic drug salbutamol enantiomers. A cellulose tris- 3-chloro-4-methylphenylcarbamate stationary phase provided the highest selectivity for this separation. The optimization of mobile phase composition allowed us to maximize the selectivity coefficient and to minimize the peak asymmetry factors. A successful salbutamol enantiomer separation is achieved at moderate modifier fraction (10–15 vol %) in the mobile phase on this column. Amine additives are necessary to suppress peak tailing. Among three tested amines isopropylamine gives the most symmetrical peaks along with the highest enantioselectivity. More... »

PAGES

1166-1175

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s1990793118070059

DOI

http://dx.doi.org/10.1134/s1990793118070059

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112462045


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