AFM-based protein fishing in the pulsed electric field View Full Text


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Article Info

DATE

2015-04

AUTHORS

Yu. D. Ivanov, T. O. Pleshakova, K. A. Malsagova, A. L. Kaysheva, A. T. Kopylov, A. A. Izotov, V. Yu. Tatur, S. G. Vesnin, N. D. Ivanova, V. S. Ziborov, A. I. Archakov

ABSTRACT

A combination of (atomic force microscopy)-based fishing (AFM-fishing) and mass spectrometry allows to capture protein molecules from solutions, concentrate and visualize them on an atomically flat surface of the AFM chip and identify by subsequent mass spectrometric analysis. In order to increase the AFM-fishing efficiency we have applied pulsed voltage with the rise time of the front of about 1 ns to the AFM chip. The AFM-chip was made using a conductive material, highly oriented pyrolytic graphite (HOPG). The increased efficiency of AFM-fishing has been demonstrated using detection of cytochrome b5 protein. Selection of the stimulating pulse with a rise time of 1 ns, corresponding to the GHz frequency range, by the effect of intrinsic emission from water observed in this frequency range during water injection into the cell. More... »

PAGES

121-129

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s1990750815020080

DOI

http://dx.doi.org/10.1134/s1990750815020080

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010520456


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