Methodological aspects of assessing chitin utilization by soil microorganisms View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-10

AUTHORS

N. A. Manucharova, A. N. Vlasenko, G. M. Zenova, T. G. Dobrovol’skaya, A. L. Stepanov

ABSTRACT

A computational method for estimating specific activity of chitin decomposition by microorganisms is proposed. Spectrophotometric and gas chromatographic methods have been used to determine the rates of chitinase production, biomass accumulation, and carbon dioxide emission by pure cultures of microorganisms grown on a chitin-containing medium. Among dominants of the chitinolytic community of chernozem (Trichoderma viride, Stretomyces albolongus, Alcaligenes, and Arthrobacter), the highest chitinolytic activity is characteristic of prokaryotes. In brown desert-steppe soil, the main destructor prokaryotes are actinomycetes (S. roseolilacinus). The biomass of the fungus T. viride growth on the chitin-containing medium markedly exceeds that of prokaryotes, but the specific activity of respiration and chitinase production in actinomycetes S. roseolilacinus and S. albolongus is an order of magnitude higher than in T. viride. More... »

PAGES

549-553

References to SciGraph publications

Journal

TITLE

Biology Bulletin

ISSUE

5

VOLUME

35

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s1062359008050191

DOI

http://dx.doi.org/10.1134/s1062359008050191

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018920227


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