Transformation of wheat with the HMW-GS 1Bx14 gene without markers View Full Text


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Article Info

DATE

2011-02

AUTHORS

X. Liu, W. Jin, J. Liu, H. Zhao, A. Guo

ABSTRACT

High molecular weight (HMW) glutenin polypeptides are critical contributors to the visco/elastic properties responsible for the processing characteristics and utilizations of wheat flour. In order to improve bread making quality of flour and produce transgenic plants free of selectable markers, a linear DNA construct consisting of a minimal expression cassette with the HMW-GS 1Bx14 gene was transformed into wheat cultivar Mianyang19 by microprojectile bombardment. The transform ants were selected by PCR instead of herbicidal markers. Seven transgenic plants were identified from a total of 1219 transformants, yielding a transformation frequency of 0.28%. An SDS-PAGE analysis confirmed that the 1Bx14 gene was expressed in three T1 seeds of the transgenic plants. Our results demonstrated that it is feasible to obtain marker-free trans-formants using the linear-expression-cassette-transformation approach coupled with PCR selection. More... »

PAGES

182-188

References to SciGraph publications

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  • 2003-05. Complete sequence analysis of transgene loci from plants transformed via microprojectile bombardment in PLANT MOLECULAR BIOLOGY
  • 2008-04. Agrobacterium-mediated transformation of maize (Zea mays) with Cre-lox site specific recombination cassettes in BIBAC vectors in PLANT MOLECULAR BIOLOGY
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  • 2000-01. Silencing of HMW glutenins in transgenic wheat expressing extra HMW subunits in THEORETICAL AND APPLIED GENETICS
  • 2007-03. Optimization of wheat co-transformation procedure with gene cassettes resulted in an improvement in transformation frequency in MOLECULAR BIOLOGY REPORTS
  • 1996-09. Integration and expression of the high-molecular-weight glutenin subunit 1Ax1 gene into wheat in NATURE BIOTECHNOLOGY
  • 2006-10. Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium-mediated transformation in MOLECULAR BREEDING
  • 1997-11. Transformation of wheat with high molecular weight subunit genes results in improved functional properties in NATURE BIOTECHNOLOGY
  • 2000-04. Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes in NATURE BIOTECHNOLOGY
  • 2000-02. Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns in TRANSGENIC RESEARCH
  • 1983-07. A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation in NATURE
  • 2006-11. Functional properties of a new low-molecular-weight glutenin subunit gene from a bread wheat cultivar in THEORETICAL AND APPLIED GENETICS
  • 2000-10. T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium-mediated transformation in MOLECULAR BREEDING
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