Isolation and functional analysis of cotton universal stress protein promoter in response to phytohormones and abiotic stresses View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-08-26

AUTHORS

Muzna Zahur, Asma Maqbool, Muhammad Irfan, Muhammad Younas Khan Barozai, Bushra Rashid, Shiekh Riazuddin, Tayyab Husnain

ABSTRACT

The 949 bp promoter fragment upstream from the translation initiation site of the GUSP gene encoding a universal stress protein was isolated from the genomic DNA of Gossypium arboreum. Some putative cis-acting elements involved in stress responses including E-box, ABRE, DPBF-box, and MYB-core elements were found in the promoter region. In an Agrobacterium-mediated transient expression assay, strong activation of the GUSP full promoter region occurred in tobacco leaves following dehydration, abscisic acid, salt, heavy metal, gibberellic acid and dark treatments. Deletion analysis of the promoter revealed that the dehydration, abscisic acid and salt responses were affected by the deletion between −208 and −949 bp and showed 2–4-fold induction. However, in response to dark, gibberellic acid and heavy metals the induction was only 2-fold. These findings further our understanding of the regulation of GUSP expression. This is an important study as no report of this universal stress protein promoter is available in literature. More... »

PAGES

578-585

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0026893309040086

DOI

http://dx.doi.org/10.1134/s0026893309040086

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014444461


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