Identification and expression of six drought-responsive transcripts through differential display in desi cotton (Gossypium arboreum) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-08-10

AUTHORS

A. Maqbool, M. Zahur, M. Irfan, M. Younas, K. Barozai, B. Rashid, T. Husnain, S. Riazuddin

ABSTRACT

There is not enough information available on drought-modulated gene(s) in Gossypium arboreum, which can be a valuable gene pool for improving modern cotton cultivars. In the present work, differential display reverse transcriptase PCR (DDRT) was used to compare overall differences in gene expression between water-stressed and control plants. By screening 93 primer-pair combinations, the DDRT technique resulted in upregulation of 30 cDNA transcripts. Through reamplification and quality control assay, ten cDNA transcripts appeared false positive. The remaining 20-cDNA transcripts were extracted from the gel, reamplified, cloned and sequenced. Homology search revealed that six transcripts showed significant homology with known genes. Real-time RT-PCR showed that, among six transcripts, five showed significant overexpression in water-stressed leaves as compared to control. This is an important finding since there are only few reports of universal stress protein, and transposable elements are available in plants but none in cotton under drought condition. More... »

PAGES

492-498

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s002689330804002x

DOI

http://dx.doi.org/10.1134/s002689330804002x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037143822


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Maqbool", 
        "givenName": "A.", 
        "id": "sg:person.01036177266.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036177266.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zahur", 
        "givenName": "M.", 
        "id": "sg:person.0770064066.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770064066.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Irfan", 
        "givenName": "M.", 
        "id": "sg:person.07554217746.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07554217746.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Younas", 
        "givenName": "M.", 
        "id": "sg:person.012302520434.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012302520434.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Barozai", 
        "givenName": "K.", 
        "id": "sg:person.0577234667.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577234667.94"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rashid", 
        "givenName": "B.", 
        "id": "sg:person.01266654266.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266654266.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Husnain", 
        "givenName": "T.", 
        "id": "sg:person.01002142717.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002142717.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Riazuddin", 
        "givenName": "S.", 
        "id": "sg:person.07501623474.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07501623474.29"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf02703703", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031068794", 
          "https://doi.org/10.1007/bf02703703"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/mb:19:2:201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006427540", 
          "https://doi.org/10.1385/mb:19:2:201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00425-005-0071-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029652038", 
          "https://doi.org/10.1007/s00425-005-0071-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00000-005-1084-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015651526", 
          "https://doi.org/10.1007/s00000-005-1084-3"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2008-08-10", 
    "datePublishedReg": "2008-08-10", 
    "description": "There is not enough information available on drought-modulated gene(s) in Gossypium arboreum, which can be a valuable gene pool for improving modern cotton cultivars. In the present work, differential display reverse transcriptase PCR (DDRT) was used to compare overall differences in gene expression between water-stressed and control plants. By screening 93 primer-pair combinations, the DDRT technique resulted in upregulation of 30 cDNA transcripts. Through reamplification and quality control assay, ten cDNA transcripts appeared false positive. The remaining 20-cDNA transcripts were extracted from the gel, reamplified, cloned and sequenced. Homology search revealed that six transcripts showed significant homology with known genes. Real-time RT-PCR showed that, among six transcripts, five showed significant overexpression in water-stressed leaves as compared to control. This is an important finding since there are only few reports of universal stress protein, and transposable elements are available in plants but none in cotton under drought condition.", 
    "genre": "article", 
    "id": "sg:pub.10.1134/s002689330804002x", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1371988", 
        "issn": [
          "0026-8933", 
          "1608-3245"
        ], 
        "name": "Molecular Biology", 
        "publisher": "Pleiades Publishing", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "42"
      }
    ], 
    "keywords": [
      "cDNA transcripts", 
      "drought-responsive transcripts", 
      "universal stress protein", 
      "differential display reverse", 
      "valuable gene pool", 
      "water-stressed leaves", 
      "primer pair combinations", 
      "modern cotton cultivars", 
      "Gossypium arboreum", 
      "transposable elements", 
      "gene pool", 
      "significant homology", 
      "control plants", 
      "homology search", 
      "differential display", 
      "gene expression", 
      "stress proteins", 
      "cotton cultivars", 
      "drought conditions", 
      "transcripts", 
      "desi cotton", 
      "real-time RT-PCR", 
      "plants", 
      "RT-PCR", 
      "significant overexpression", 
      "quality control assays", 
      "expression", 
      "cotton", 
      "arboreum", 
      "control assays", 
      "homology", 
      "genes", 
      "leaves", 
      "protein", 
      "overexpression", 
      "cultivars", 
      "upregulation", 
      "PCR", 
      "reamplification", 
      "assays", 
      "pool", 
      "identification", 
      "overall difference", 
      "gel", 
      "important finding", 
      "present work", 
      "elements", 
      "reverse", 
      "combination", 
      "findings", 
      "display", 
      "conditions", 
      "differences", 
      "enough information", 
      "information", 
      "report", 
      "search", 
      "work", 
      "technique", 
      "display reverse", 
      "DDRT technique"
    ], 
    "name": "Identification and expression of six drought-responsive transcripts through differential display in desi cotton (Gossypium arboreum)", 
    "pagination": "492-498", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037143822"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1134/s002689330804002x"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1134/s002689330804002x", 
      "https://app.dimensions.ai/details/publication/pub.1037143822"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:19", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_474.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1134/s002689330804002x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1134/s002689330804002x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1134/s002689330804002x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1134/s002689330804002x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1134/s002689330804002x'


 

This table displays all metadata directly associated to this object as RDF triples.

184 TRIPLES      22 PREDICATES      90 URIs      78 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1134/s002689330804002x schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N4f29d214eea24675858c5e2d508b2d24
4 schema:citation sg:pub.10.1007/bf02703703
5 sg:pub.10.1007/s00000-005-1084-3
6 sg:pub.10.1007/s00425-005-0071-5
7 sg:pub.10.1385/mb:19:2:201
8 schema:datePublished 2008-08-10
9 schema:datePublishedReg 2008-08-10
10 schema:description There is not enough information available on drought-modulated gene(s) in Gossypium arboreum, which can be a valuable gene pool for improving modern cotton cultivars. In the present work, differential display reverse transcriptase PCR (DDRT) was used to compare overall differences in gene expression between water-stressed and control plants. By screening 93 primer-pair combinations, the DDRT technique resulted in upregulation of 30 cDNA transcripts. Through reamplification and quality control assay, ten cDNA transcripts appeared false positive. The remaining 20-cDNA transcripts were extracted from the gel, reamplified, cloned and sequenced. Homology search revealed that six transcripts showed significant homology with known genes. Real-time RT-PCR showed that, among six transcripts, five showed significant overexpression in water-stressed leaves as compared to control. This is an important finding since there are only few reports of universal stress protein, and transposable elements are available in plants but none in cotton under drought condition.
11 schema:genre article
12 schema:inLanguage en
13 schema:isAccessibleForFree false
14 schema:isPartOf N0ce2ae3e4e0a4a52bc46abadd86c401c
15 N86660b1313254d4ab8cc207b9c5fd286
16 sg:journal.1371988
17 schema:keywords DDRT technique
18 Gossypium arboreum
19 PCR
20 RT-PCR
21 arboreum
22 assays
23 cDNA transcripts
24 combination
25 conditions
26 control assays
27 control plants
28 cotton
29 cotton cultivars
30 cultivars
31 desi cotton
32 differences
33 differential display
34 differential display reverse
35 display
36 display reverse
37 drought conditions
38 drought-responsive transcripts
39 elements
40 enough information
41 expression
42 findings
43 gel
44 gene expression
45 gene pool
46 genes
47 homology
48 homology search
49 identification
50 important finding
51 information
52 leaves
53 modern cotton cultivars
54 overall difference
55 overexpression
56 plants
57 pool
58 present work
59 primer pair combinations
60 protein
61 quality control assays
62 real-time RT-PCR
63 reamplification
64 report
65 reverse
66 search
67 significant homology
68 significant overexpression
69 stress proteins
70 technique
71 transcripts
72 transposable elements
73 universal stress protein
74 upregulation
75 valuable gene pool
76 water-stressed leaves
77 work
78 schema:name Identification and expression of six drought-responsive transcripts through differential display in desi cotton (Gossypium arboreum)
79 schema:pagination 492-498
80 schema:productId N8c85c808120541618b45b7a0b6eeabbe
81 Nd23af096a0444318b260bd4b41f18b87
82 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037143822
83 https://doi.org/10.1134/s002689330804002x
84 schema:sdDatePublished 2022-01-01T18:19
85 schema:sdLicense https://scigraph.springernature.com/explorer/license/
86 schema:sdPublisher N3209615a0dc24089b210284e0dace73d
87 schema:url https://doi.org/10.1134/s002689330804002x
88 sgo:license sg:explorer/license/
89 sgo:sdDataset articles
90 rdf:type schema:ScholarlyArticle
91 N0ce2ae3e4e0a4a52bc46abadd86c401c schema:issueNumber 4
92 rdf:type schema:PublicationIssue
93 N154d7550ad804f1699e2ec4b82ee372f rdf:first sg:person.01266654266.61
94 rdf:rest Ncbb22937a2694f6d9ea248c8493f4aa8
95 N2397d1fef86c43c592e124753f93f5ea rdf:first sg:person.07554217746.34
96 rdf:rest N693d58d1c58b405292f48b89d00e5a39
97 N3209615a0dc24089b210284e0dace73d schema:name Springer Nature - SN SciGraph project
98 rdf:type schema:Organization
99 N4319092427e44b9f915c059359db8f47 rdf:first sg:person.07501623474.29
100 rdf:rest rdf:nil
101 N4f29d214eea24675858c5e2d508b2d24 rdf:first sg:person.01036177266.76
102 rdf:rest Nd5cc5edf00774cba8d9bb71f17559f43
103 N6027d7e0c8c04413b2ad71970f5b2852 rdf:first sg:person.0577234667.94
104 rdf:rest N154d7550ad804f1699e2ec4b82ee372f
105 N693d58d1c58b405292f48b89d00e5a39 rdf:first sg:person.012302520434.10
106 rdf:rest N6027d7e0c8c04413b2ad71970f5b2852
107 N86660b1313254d4ab8cc207b9c5fd286 schema:volumeNumber 42
108 rdf:type schema:PublicationVolume
109 N8c85c808120541618b45b7a0b6eeabbe schema:name doi
110 schema:value 10.1134/s002689330804002x
111 rdf:type schema:PropertyValue
112 Ncbb22937a2694f6d9ea248c8493f4aa8 rdf:first sg:person.01002142717.98
113 rdf:rest N4319092427e44b9f915c059359db8f47
114 Nd23af096a0444318b260bd4b41f18b87 schema:name dimensions_id
115 schema:value pub.1037143822
116 rdf:type schema:PropertyValue
117 Nd5cc5edf00774cba8d9bb71f17559f43 rdf:first sg:person.0770064066.54
118 rdf:rest N2397d1fef86c43c592e124753f93f5ea
119 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
120 schema:name Biological Sciences
121 rdf:type schema:DefinedTerm
122 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
123 schema:name Genetics
124 rdf:type schema:DefinedTerm
125 sg:journal.1371988 schema:issn 0026-8933
126 1608-3245
127 schema:name Molecular Biology
128 schema:publisher Pleiades Publishing
129 rdf:type schema:Periodical
130 sg:person.01002142717.98 schema:affiliation grid-institutes:grid.11173.35
131 schema:familyName Husnain
132 schema:givenName T.
133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002142717.98
134 rdf:type schema:Person
135 sg:person.01036177266.76 schema:affiliation grid-institutes:grid.11173.35
136 schema:familyName Maqbool
137 schema:givenName A.
138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036177266.76
139 rdf:type schema:Person
140 sg:person.012302520434.10 schema:affiliation grid-institutes:grid.11173.35
141 schema:familyName Younas
142 schema:givenName M.
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012302520434.10
144 rdf:type schema:Person
145 sg:person.01266654266.61 schema:affiliation grid-institutes:grid.11173.35
146 schema:familyName Rashid
147 schema:givenName B.
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266654266.61
149 rdf:type schema:Person
150 sg:person.0577234667.94 schema:affiliation grid-institutes:grid.11173.35
151 schema:familyName Barozai
152 schema:givenName K.
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577234667.94
154 rdf:type schema:Person
155 sg:person.07501623474.29 schema:affiliation grid-institutes:grid.11173.35
156 schema:familyName Riazuddin
157 schema:givenName S.
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07501623474.29
159 rdf:type schema:Person
160 sg:person.07554217746.34 schema:affiliation grid-institutes:grid.11173.35
161 schema:familyName Irfan
162 schema:givenName M.
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07554217746.34
164 rdf:type schema:Person
165 sg:person.0770064066.54 schema:affiliation grid-institutes:grid.11173.35
166 schema:familyName Zahur
167 schema:givenName M.
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770064066.54
169 rdf:type schema:Person
170 sg:pub.10.1007/bf02703703 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031068794
171 https://doi.org/10.1007/bf02703703
172 rdf:type schema:CreativeWork
173 sg:pub.10.1007/s00000-005-1084-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015651526
174 https://doi.org/10.1007/s00000-005-1084-3
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/s00425-005-0071-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029652038
177 https://doi.org/10.1007/s00425-005-0071-5
178 rdf:type schema:CreativeWork
179 sg:pub.10.1385/mb:19:2:201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006427540
180 https://doi.org/10.1385/mb:19:2:201
181 rdf:type schema:CreativeWork
182 grid-institutes:grid.11173.35 schema:alternateName Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan
183 schema:name Center of Excellence in Molecular Biology, University of Punjab, 87-Canal Bank Road, 53700, Thokar Niaz Baig Lahore, Pakistan
184 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...